Restriction map for
pFN21AB5868
(Circular) (Six-base) MAPSORT of: pFN21AB5868.seq Check: 5137 from: 1 to: 6184
pFN21AB5868 6184 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2643 3186 3527 1937
Size: 706 543 341 4594
Fragments arranged by size:
4594 706 543 341
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4247 5631 5899 1632
Size: 2615 1384 268 1917
Fragments arranged by size:
2615 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 2511 2996 5083 6060 1203
Size: 1308 485 2087 977 1327
Fragments arranged by size:
2087 1327 1308 977 485
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2620 1184
Size: 1436 4748
AlwNI CAG_nnn'CTG
Cuts at: 2387 3927 5499 2387
Size: 1540 1572 3072
Fragments arranged by size:
3072 1572 1540
ApaLI G'TGCA_C
Cuts at: 2533 4241 5397 2533
Size: 1708 1156 3320
Fragments arranged by size:
3320 1708 1156
ApoI r'AATT_y
Cuts at: 1493 2948 3493 3719 3871 5980 1493
Size: 1455 545 226 152 2109 1697
Fragments arranged by size:
2109 1697 1455 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2345 3509 1870
Size: 73 402 1164 4545
Fragments arranged by size:
4545 1164 402 73
BamHI G'GATC_C
Cuts at: 2110 2588 3514 3881 2110
Size: 478 926 367 4413
Fragments arranged by size:
4413 926 478 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3505 4960 618
Size: 325 217 627 1718 1455 1842
Fragments arranged by size:
1842 1718 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3503 1391
Size: 2112 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 928
Size: 971 990 4223
Fragments arranged by size:
4223 990 971
BbvCI CC'TCA_GC
Cuts at: 2256 2442 2256
Size: 186 5998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2519 2553 3120 3154 4375 4409 1903
Size: 34 582 34 567 34 1221 34 3678
Fragments arranged by size:
3678 1221 582 567 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2198 2483 2966 4076 5286 2198
Size: 285 483 1110 1210 3096
Fragments arranged by size:
3096 1210 1110 483 285
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2799 2858 3601 2799
Size: 59 743 5382
Fragments arranged by size:
5382 743 59
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2609 2966 4245 5401 1790
Size: 747 72 357 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 747 357 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3137 3169 3915
Size: 416 53 83 399 24 1720 32 746
Cuts at: 3915 4873 442
Size: 958 1753
Fragments arranged by size:
1753 1720 958 746 416 399 83 53
32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2225
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3419 4844 1239
Size: 2180 1425 2579
Fragments arranged by size:
2579 2180 1425
Bpu10I CC'TnA_GC
Cuts at: 2256 2442 2256
Size: 186 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2890 4309 5174 5472 2890
Size: 1419 865 298 3602
Fragments arranged by size:
3602 1419 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3014 4847 882
Size: 617 1515 1833 2219
Fragments arranged by size:
2219 1833 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 2079 3975 493
Size: 711 875 1896 2702
Fragments arranged by size:
2702 1896 875 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2650 2680 1178
Size: 30 165 30 1247 30 4682
Fragments arranged by size:
4682 1247 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6184
BseYI C'CCAG_C
Cuts at: 2431 5387 5883 2431
Size: 2956 496 2732
Fragments arranged by size:
2956 2732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2502 2740 1163
Size: 573 766 238 4607
Fragments arranged by size:
4607 766 573 238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 2800 3357 4245 4330 5401 2537
Size: 263 557 888 85 1071 3320
Fragments arranged by size:
3320 1071 888 557 263 85
BsmI GAATG_Cn'
Cuts at: 2448 2900 3038 3695 3788 2448
Size: 452 138 657 93 4844
Fragments arranged by size:
4844 657 452 138 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2609 2800 2966 3357 3503 4245
Size: 399 747 72 191 166 391 146 742
Cuts at: 4245 4330 5401 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 391 191 166
146 85 72
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 4073 5803 4073
Size: 1730 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2765 3539 844
Size: 1921 774 3489
Fragments arranged by size:
3489 1921 774
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2093 2132 3351 4679 4853 65
Size: 1183 845 39 1219 1328 174 1396
Fragments arranged by size:
1396 1328 1219 1183 845 174 39
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 3356 4244 5256 3356
Size: 888 1012 4284
Fragments arranged by size:
4284 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 2110 2588 3103 3514 3881 4266 4283 5735
Size: 138 478 515 411 367 385 17 1452
Cuts at: 5735 5821 5833 6179 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 515 478 411 385 367 346
138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2287 2287
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 2535 2642 2810 2869 3700 4494 4514 2535
Size: 107 168 59 831 794 20 4205
Fragments arranged by size:
4205 831 794 168 107 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2287 2322 3447 3482 529
Size: 35 1723 35 1125 35 3231
Fragments arranged by size:
3231 1723 1125 35 35 35
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3330 5191 809
Size: 507 2014 1861 1802
Fragments arranged by size:
2014 1861 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2401 3631
Size: 54 1425 50 275 18 531 40 1230
Cuts at: 3631 4520 6149 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1230 889 531 275 54 50
43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4114 1353
Size: 2761 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2440 4771 5145 5291 387
Size: 1064 138 851 2331 374 146 1280
Fragments arranged by size:
2331 1280 1064 851 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3419 4247 4844 5631 5899 1239
Size: 393 1787 828 597 787 268 1524
Fragments arranged by size:
1787 1524 828 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2384 3426 2384
Size: 1042 5142
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
EcoRV GAT'ATC
Cuts at: 2618 2618
Size: 6184
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2045 5331 1678
Size: 367 3286 2531
Fragments arranged by size:
3286 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3372 3404 4825 4857 4899
Size: 32 135 32 1800 32 1421 32 42
Cuts at: 4899 4931 1373
Size: 32 2626
Fragments arranged by size:
2626 1800 1421 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2191 3528 3774 669
Size: 988 281 253 1337 246 3079
Fragments arranged by size:
3079 1337 988 281 253 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 2735 3783 2735
Size: 1048 5136
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 3035 5298 5482 1305
Size: 1176 554 2263 184 2007
Fragments arranged by size:
2263 2007 1176 554 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3831
Fragments arranged by size:
3831 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2602 2959 4142 4501 4660 518
Size: 648 1436 357 1183 359 159 2042
Fragments arranged by size:
2042 1436 1183 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 2435 3600 4277 5425 5670 1606
Size: 829 1165 677 1148 245 2120
Fragments arranged by size:
2120 1165 1148 829 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 2601 3000 3542 5087 1132
Size: 97 1372 399 542 1545 2229
Fragments arranged by size:
2229 1545 1372 542 399 97
PasI CC'CwG_GG
Cuts at: 1348 2968 1348
Size: 1620 4564
PciI A'CATG_T
Cuts at: 2996 5083 2996
Size: 2087 4097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PfoI T'CCnGG_A
Cuts at: 2611 2611
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3426 3426
Size: 6184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 2377 2542 3536 830
Size: 1547 165 994 3478
Fragments arranged by size:
3478 1547 994 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2435 2435
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2373 2538 2554 3532 4667 5348 5539
Size: 219 1328 165 16 978 1135 681 191
Cuts at: 5539 826
Size: 1471
Fragments arranged by size:
1471 1328 1135 978 681 219 191 165
16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2347 3511 1872
Size: 475 1164 4545
Fragments arranged by size:
4545 1164 475
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2905 4288 5189 5451 820
Size: 197 926 962 1383 901 262 1553
Fragments arranged by size:
1553 1383 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6184
StyI C'CwwG_G
Cuts at: 513 1065 3623 513
Size: 552 2558 3074
Fragments arranged by size:
3074 2558 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2964 2996 3035 3067 6144 6176 2964
Size: 32 39 32 3077 32 2972
Fragments arranged by size:
3077 2972 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2153 1316
Size: 837 5347
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2723 2723
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2345 3509 1870
Size: 475 1164 4545
Fragments arranged by size:
4545 1164 475
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI