Restriction map for
pFN21AB5866
(Circular) (Six-base) MAPSORT of: pFN21AB5866.seq Check: 6777 from: 1 to: 6178
pFN21AB5866 6178 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 3521 1937
Size: 871 713 4594
Fragments arranged by size:
4594 871 713
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 3202 4241 5625 5893 1632
Size: 478 1092 1039 1384 268 1917
Fragments arranged by size:
1917 1384 1092 1039 478 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 2445 5077 6054 1203
Size: 1242 2632 977 1327
Fragments arranged by size:
2632 1327 1242 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AlwNI CAG_nnn'CTG
Cuts at: 3921 5493 3921
Size: 1572 4606
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 3487 3713 3865 5974 1493
Size: 1994 226 152 2109 1697
Fragments arranged by size:
2109 1994 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 1870
Size: 73 1560 4545
Fragments arranged by size:
4545 1560 73
BamHI G'GATC_C
Cuts at: 2893 3508 3875 2893
Size: 615 367 5196
Fragments arranged by size:
5196 615 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3499 4954 618
Size: 325 217 627 1712 1455 1842
Fragments arranged by size:
1842 1712 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2224 3497 1391
Size: 833 1273 4072
Fragments arranged by size:
4072 1273 833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 3291 928
Size: 971 383 1009 3815
Fragments arranged by size:
3815 1009 971 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4789 136
Size: 107 122 71 631 605 3117 1525
Fragments arranged by size:
3117 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3111 6173 3111
Size: 3062 3116
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 2134 4239 5395 1790
Size: 344 2105 1156 2573
Fragments arranged by size:
2573 2105 1156 344
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3355 3909 4867
Size: 416 53 83 399 24 1938 554 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1938 1753 958 554 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 4838 1239
Size: 1062 2537 2579
Fragments arranged by size:
2579 2537 1062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4841 882
Size: 617 3342 2219
Fragments arranged by size:
3342 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 3969 493
Size: 711 2021 744 2702
Fragments arranged by size:
2702 2021 744 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3042 3072 1178
Size: 30 165 30 1639 30 4284
Fragments arranged by size:
4284 1639 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2416 2512 1186
Size: 943 287 96 4852
Fragments arranged by size:
4852 943 287 96
BseYI C'CCAG_C
Cuts at: 2174 5381 5877 2174
Size: 3207 496 2475
Fragments arranged by size:
3207 2475 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5605
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4239 4324 5395 4239
Size: 85 1071 5022
Fragments arranged by size:
5022 1071 85
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2224 3497 4239 4324 5395 1391
Size: 399 344 90 1273 742 85 1071 2174
Fragments arranged by size:
2174 1273 1071 742 399 344 90 85
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 844
Size: 2689 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3140 4822 5009 1700
Size: 110 1330 1682 187 2869
Fragments arranged by size:
2869 1682 1330 187 110
BsrGI T'GTAC_A
Cuts at: 96 3085 96
Size: 2989 3189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2682 794
Size: 1888 4290
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2893 3111 3259 3508 3875 4260 4277 5729
Size: 921 218 148 249 367 385 17 1452
Cuts at: 5729 5815 5827 6173 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 921 385 367 346 249 218
148 86 17 12
BstZ17I GTA'TAC
Cuts at: 2809 2809
Size: 6178
Bsu36I CC'TnA_GG
Cuts at: 2180 2180
Size: 6178
BtgI C'CryG_G
Cuts at: 513 1065 2134 513
Size: 552 1069 4557
Fragments arranged by size:
4557 1069 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4488 4508 3694
Size: 794 20 5364
Fragments arranged by size:
5364 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2789 5185 809
Size: 507 1473 2396 1802
Fragments arranged by size:
2396 1802 1473 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3625 4514 6143
Size: 54 1425 50 275 18 1795 889 1629
Cuts at: 6143 8
Size: 43
Fragments arranged by size:
1795 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2160 2434 4108 1353
Size: 800 7 274 1674 3423
Fragments arranged by size:
3423 1674 800 274 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2301 3202 4241 4838 5625 5893
Size: 393 478 191 901 1039 597 787 268
Cuts at: 5893 1239
Size: 1524
Fragments arranged by size:
1524 1039 901 787 597 478 393 268
191
EcoO109I rG'GnC_Cy
Cuts at: 3011 3459 3011
Size: 448 5730
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 5325 1678
Size: 3647 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3149 3181 4819 4851 4893
Size: 32 135 32 1577 32 1638 32 42
Cuts at: 4893 4925 1373
Size: 32 2626
Fragments arranged by size:
2626 1638 1577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 2875 3252 3522 3768 669
Size: 988 281 166 771 377 270 246 3079
Fragments arranged by size:
3079 988 771 377 281 270 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 3252 3768 3252
Size: 516 5662
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5292 5476 1305
Size: 3987 184 2007
Fragments arranged by size:
3987 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4356
Fragments arranged by size:
4356 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4136 4495 4654 518
Size: 648 2970 359 159 2042
Fragments arranged by size:
2970 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2240 3172 3594 4271 5419 5664 1606
Size: 634 932 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 677 634 422 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5626
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2449 3214 3536 5081 1132
Size: 97 1220 765 322 1545 2229
Fragments arranged by size:
2229 1545 1220 765 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 2445 5077 2445
Size: 2632 3546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6178
PfoI T'CCnGG_A
Cuts at: 2607 2607
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3011 3011
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PstI C_TGCA'G
Cuts at: 830 3530 830
Size: 2700 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 2240 3172 2240
Size: 932 5246
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SanDI GG'GwC_CC
Cuts at: 3011 3011
Size: 6178
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 2519 4426 6138 2519
Size: 1907 1712 2559
Fragments arranged by size:
2559 1907 1712
SexAI A'CCwGG_T
Cuts at: 2959 2959
Size: 6178
SfcI C'TryA_G
Cuts at: 826 1045 2325 3526 4661 5342 5533 826
Size: 219 1280 1201 1135 681 191 1471
Fragments arranged by size:
1471 1280 1201 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4282 5183 5445 820
Size: 197 926 2339 901 262 1553
Fragments arranged by size:
2339 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 2669 2701 2969 4102 5
Size: 47 2617 32 268 1133 2081
Fragments arranged by size:
2617 2081 1133 268 47 32
StuI AGG'CCT
Cuts at: 1517 2185 1517
Size: 668 5510
StyI C'CwwG_G
Cuts at: 513 1065 2095 3617 513
Size: 552 1030 1522 3074
Fragments arranged by size:
3074 1522 1030 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4205 4222 4375 4560 5005 325
Size: 1255 2625 17 153 185 445 1498
Fragments arranged by size:
2625 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 2517 3085
Size: 274 80 33 51 1443 465 75 568
Cuts at: 3085 3357 4424 6136 96
Size: 272 1067 1712 138
Fragments arranged by size:
1712 1443 1067 568 465 274 272 138
80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2734 2766 2829 2861 6138 6170 2734
Size: 32 63 32 3277 32 2742
Fragments arranged by size:
3277 2742 63 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2967 2011
Size: 956 5222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI