Restriction map for
pFN21AB5865
(Circular) (Six-base) MAPSORT of: pFN21AB5865.seq Check: 8416 from: 1 to: 6088
pFN21AB5865 6088 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3233 3233
Size: 6088
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2225 2273 2525 2948 3395 4151 5535
Size: 497 96 48 252 423 447 756 1384
Cuts at: 5535 5803 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 756 497 447 423 268 252
96 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 2981 1166
Size: 1815 4273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2999 1184
Size: 1815 4273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 6056
AlwNI CAG_nnn'CTG
Cuts at: 2020 2107 2668 2821 2926 2950 3205 3298 3349
Size: 87 561 153 105 24 255 93 51
Cuts at: 3349 3831 5403 2020
Size: 482 1572 2705
Fragments arranged by size:
2705 1572 561 482 255 153 105 93
87 51 24
ApaLI G'TGCA_C
Cuts at: 2403 4145 5301 2403
Size: 1742 1156 3190
Fragments arranged by size:
3190 1742 1156
ApoI r'AATT_y
Cuts at: 1493 2414 3397 3623 3775 5884 1493
Size: 921 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 921 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 3170 3413 1870
Size: 73 990 237 243 4545
Fragments arranged by size:
4545 990 243 237 73
BamHI G'GATC_C
Cuts at: 2267 3418 3785 2267
Size: 1151 367 4570
Fragments arranged by size:
4570 1151 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2724 2812 3359 3407 1391
Size: 1333 88 547 48 4072
Fragments arranged by size:
4072 1333 547 88 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 2678 928
Size: 971 558 221 4338
Fragments arranged by size:
4338 971 558 221
BbvCI CC'TCA_GC
Cuts at: 2469 2469
Size: 6088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3077 3111 3161 3195 4279 4313 1903
Size: 34 1140 34 50 34 1084 34 3678
Fragments arranged by size:
3678 1140 1084 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 2568 2697 1548
Size: 1020 129 4939
Fragments arranged by size:
4939 1020 129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3054 3505 3054
Size: 451 5637
Bme1580I G_kGCm'C
Cuts at: 1790 2407 2978 4149 5305 1790
Size: 617 571 1171 1156 2573
Fragments arranged by size:
2573 1171 1156 617 571
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2304 3215 3819
Size: 416 53 83 399 24 887 911 604
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 958 911 887 604 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2578 3329 4748 1239
Size: 986 353 751 1419 2579
Fragments arranged by size:
2579 1419 986 751 353
Bpu10I CC'TnA_GC
Cuts at: 2469 2469
Size: 6088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 4213 5078 5376 2381
Size: 1832 865 298 3093
Fragments arranged by size:
3093 1832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2275 3879 493
Size: 711 1071 1604 2702
Fragments arranged by size:
2702 1604 1071 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3210 3895 4277 4912
Size: 53 83 186 936 1677 685 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1677 1451 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3020 3050 1178
Size: 30 165 30 1617 30 4216
Fragments arranged by size:
4216 1617 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 2195 2198 3059 3062 1186
Size: 845 164 3 861 3 4212
Fragments arranged by size:
4212 861 845 164 3 3
BseYI C'CCAG_C
Cuts at: 2752 2913 3252 3352 5291 5787 2752
Size: 161 339 100 1939 496 3053
Fragments arranged by size:
3053 1939 496 339 161 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2984 1163
Size: 573 1248 4267
Fragments arranged by size:
4267 1248 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2407 2888 2988 4149 4234 5305 2407
Size: 481 100 1161 85 1071 3190
Fragments arranged by size:
3190 1161 1071 481 100 85
BsiWI C'GTAC_G
Cuts at: 2275 2275
Size: 6088
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2407 2724 2812 2888 2978 2988 3359
Size: 399 617 317 88 76 90 10 371
Cuts at: 3359 3407 4149 4234 5305 1391
Size: 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 399 371 317 90
88 85 76 48 10
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 2935 3233 3443 844
Size: 1832 259 298 210 3489
Fragments arranged by size:
3489 1832 298 259 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2851 3975 1394
Size: 429 1028 1124 3507
Fragments arranged by size:
3507 1124 1028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2726 4148 5160 2726
Size: 1422 1012 3654
Fragments arranged by size:
3654 1422 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2668 2821 794
Size: 1874 153 4061
Fragments arranged by size:
4061 1874 153
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 3123 3123
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2267 2392 3418 3785 4170 4187 5639 5725
Size: 295 125 1026 367 385 17 1452 86
Cuts at: 5725 5737 6083 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 295 125
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2951 2951
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3763
Fragments arranged by size:
3763 939 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2853 3005 3604 4398 4418 2853
Size: 152 599 794 20 4523
Fragments arranged by size:
4523 794 599 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2835 3270
Size: 54 1425 50 275 18 240 765 435
Cuts at: 3270 3300 3535 4424 6053 8
Size: 30 235 889 1629 43
Fragments arranged by size:
1629 1425 889 765 435 275 240 235
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 2489 2598 4018 1353
Size: 779 357 109 1420 3423
Fragments arranged by size:
3423 1420 779 357 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2477 4675 5049 5195 387
Size: 1064 138 888 2198 374 146 1280
Fragments arranged by size:
2198 1280 1064 888 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2225 2273 2525 2578 2948 3329
Size: 393 497 96 48 252 53 370 381
Cuts at: 3329 3395 4151 4748 5535 5803 1239
Size: 66 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 497 393 381 370
268 252 96 66 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2729 2963 2729
Size: 234 5854
EcoO109I rG'GnC_Cy
Cuts at: 2236 2665 2869 2947 2955 3247 2236
Size: 429 204 78 8 292 5077
Fragments arranged by size:
5077 429 292 204 78 8
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
EcoRV GAT'ATC
Cuts at: 2365 2365
Size: 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2530 2500
Size: 30 6058
FspI TGC'GCA
Cuts at: 2661 4594 2661
Size: 1933 4155
HaeII r_GCGC'y
Cuts at: 1678 2760 3167 5235 1678
Size: 1082 407 2068 2531
Fragments arranged by size:
2531 2068 1082 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 4729 4761 4803
Size: 32 135 32 454 32 2671 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2671 2626 454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3364 3432 3678 669
Size: 988 281 1426 68 246 3079
Fragments arranged by size:
3079 1426 988 281 246 68
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 3272 3302 10
Size: 54 1425 343 1005 435 30 2796
Fragments arranged by size:
2796 1425 1005 435 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 4046 4405 4564 518
Size: 648 1815 1065 359 159 2042
Fragments arranged by size:
2042 1815 1065 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3504 4181 5329 5574 1606
Size: 1311 587 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 677 587 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2053 2551 3446 4991 1132
Size: 97 824 498 895 1545 2229
Fragments arranged by size:
2229 1545 895 824 498 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2650 3160 3298 1108
Size: 1542 510 138 3898
Fragments arranged by size:
3898 1542 510 138
PfoI T'CCnGG_A
Cuts at: 2202 3342 2202
Size: 1140 4948
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 3020 3052 4157 4189 5670 5702 2315
Size: 32 673 32 1105 32 1481 32 2701
Fragments arranged by size:
2701 1481 1105 673 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2665 2869 2947 2955 3247 2665
Size: 204 78 8 292 5506
Fragments arranged by size:
5506 292 204 78 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2266 2817 2946 3090 3201 3282 3318 3440
Size: 1436 551 129 144 111 81 36 122
Cuts at: 3440 830
Size: 3478
Fragments arranged by size:
3478 1436 551 144 129 122 111 81
36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2132 2489 2132
Size: 357 5731
SbfI CC_TGCA'GG
Cuts at: 2266 3282 3440 2266
Size: 1016 158 4914
Fragments arranged by size:
4914 1016 158
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 3018 3291 3018
Size: 273 5815
SfcI C'TryA_G
Cuts at: 826 1045 2262 2813 2942 3086 3197 3278 3314
Size: 219 1217 551 129 144 111 81 36
Cuts at: 3314 3436 4571 5252 5443 826
Size: 122 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 681 551 219 191 144
129 122 111 81 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 4192 5093 5355 820
Size: 197 926 453 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 2053 3446 1132
Size: 921 1393 3774
Fragments arranged by size:
3774 1393 921
SspI AAT'ATT
Cuts at: 5 52 2636 4012 5
Size: 47 2584 1376 2081
Fragments arranged by size:
2584 2081 1376 47
StuI AGG'CCT
Cuts at: 1517 3120 1517
Size: 1603 4485
StyI C'CwwG_G
Cuts at: 513 1065 2004 2778 3527 513
Size: 552 939 774 749 3074
Fragments arranged by size:
3074 939 774 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4115 4132 4285 4470 4915 325
Size: 1255 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3195 3227 6048 6080 3195
Size: 32 2821 32 3203
Fragments arranged by size:
3203 2821 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3020 1316
Size: 1704 4384
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2785 2596
Size: 189 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 3035 4217 3035
Size: 1182 4906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI