Restriction map for
pFN21AB5861
(Circular) (Six-base) MAPSORT of: pFN21AB5861.seq Check: 1222 from: 1 to: 6088
pFN21AB5861 6088 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 2163 3409 2163
Size: 1246 4842
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3318 4151 5535 5803 1632
Size: 1686 833 1384 268 1917
Fragments arranged by size:
1917 1686 1384 833 268
AfeI AGC'GCT
Cuts at: 2537 2537
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 2549 820
Size: 197 1532 4359
Fragments arranged by size:
4359 1532 197
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 2160 4919 2160
Size: 2759 3329
AhdI GACnn_n'nnGTC
Cuts at: 2133 4817 2133
Size: 2684 3404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2797 3385 3831 5403 2797
Size: 588 446 1572 3482
Fragments arranged by size:
3482 1572 588 446
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 6088
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2490 3072 3350 3413 1870
Size: 73 79 468 582 278 63 4545
Fragments arranged by size:
4545 582 468 278 79 73 63
BamHI G'GATC_C
Cuts at: 2374 3418 3785 2374
Size: 1044 367 4677
Fragments arranged by size:
4677 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2163 2640 3373 3409
Size: 325 217 627 314 62 477 733 36
Cuts at: 3409 4864 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 733 627 477 325 314 217
62 36
BanII G_rGCy'C
Cuts at: 1391 2022 2313 3407 1391
Size: 631 291 1094 4072
Fragments arranged by size:
4072 1094 631 291
BbeI G_GCGC'C
Cuts at: 2105 2105
Size: 6088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5117
BbvCI CC'TCA_GC
Cuts at: 3044 3107 3044
Size: 63 6025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4279 4313 1903
Size: 34 50 34 2258 34 3678
Fragments arranged by size:
3678 2258 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3077 4699
Size: 107 122 71 631 605 406 999 1622
Cuts at: 4699 136
Size: 1525
Fragments arranged by size:
1622 1525 999 631 605 406 122 107
71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 2258 3505 2258
Size: 1247 4841
Bme1580I G_kGCm'C
Cuts at: 1790 2022 3378 4149 5305 1790
Size: 232 1356 771 1156 2573
Fragments arranged by size:
2573 1356 1156 771 232
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 5116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 2594 2677
Size: 416 53 83 399 24 1080 97 83
Cuts at: 2677 3819 4777 442
Size: 1142 958 1753
Fragments arranged by size:
1753 1142 1080 958 416 399 97 83
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2048 2048
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2153 2210 2486 2597 2819 2843 2966
Size: 872 42 57 276 111 222 24 123
Cuts at: 2966 3170 3288 4748 1239
Size: 204 118 1460 2579
Fragments arranged by size:
2579 1460 872 276 222 204 123 118
111 57 42 24
Bpu10I CC'TnA_GC
Cuts at: 3044 3107 3044
Size: 63 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 2333 3248 4213 5078 5376 2297
Size: 36 915 965 865 298 3009
Fragments arranged by size:
3009 965 915 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 4751 882
Size: 617 612 2640 2219
Fragments arranged by size:
2640 2219 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2364 3895 4277
Size: 53 83 186 936 569 262 1531 382
Cuts at: 4277 4912 275
Size: 635 1451
Fragments arranged by size:
1531 1451 936 635 569 382 262 186
83 53
BsaWI w'CCGG_w
Cuts at: 2160 4521 4919 5193 5340 5751 2160
Size: 2361 398 274 147 411 2497
Fragments arranged by size:
2497 2361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2816 2846 1178
Size: 30 165 30 1413 30 4420
Fragments arranged by size:
4420 1413 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2606 2717 2864 2888 2891 2963 3074
Size: 943 477 111 147 24 3 72 111
Cuts at: 3074 1186
Size: 4200
Fragments arranged by size:
4200 943 477 147 111 111 72 24
3
BseYI C'CCAG_C
Cuts at: 3000 3099 3298 3378 5291 5787 3000
Size: 99 199 80 1913 496 3301
Fragments arranged by size:
3301 1913 496 199 99 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3170 1163
Size: 573 1434 4081
Fragments arranged by size:
4081 1434 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 2652 4149 4234 5305 2313
Size: 339 1497 85 1071 3096
Fragments arranged by size:
3096 1497 1071 339 85
BsmI GAATG_Cn'
Cuts at: 3094 3599 3692 3094
Size: 505 93 5490
Fragments arranged by size:
5490 505 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2557 2186
Size: 371 5717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2313 2652 3378 3407 4149 4234
Size: 399 232 291 339 726 29 742 85
Cuts at: 4234 5305 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 726 399 339 291 232
85 29
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2521 3977 5707 2521
Size: 1456 1730 2902
Fragments arranged by size:
2902 1730 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2250 3355 3975 1394
Size: 429 427 1105 620 3507
Fragments arranged by size:
3507 1105 620 429 427
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 2637 3308 4732 4919 1700
Size: 110 350 477 671 1424 187 2869
Fragments arranged by size:
2869 1424 671 477 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 2383 96
Size: 2287 3801
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2558 2936 4148 5160 2558
Size: 378 1212 1012 3486
Fragments arranged by size:
3486 1212 1012 378
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3181 794
Size: 2387 3701
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 3311 1184
Size: 2127 3961
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 2821
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2374 3418 3785 4170 4187 5639 5725 5737
Size: 402 1044 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1044 402 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3252 3363 3252
Size: 111 5977
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 3604 4398 4418 3604
Size: 794 20 5274
Fragments arranged by size:
5274 794 20
ClaI AT'CG_AT
Cuts at: 1554 2181 1554
Size: 627 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 3535 4424
Size: 54 1425 50 275 18 654 1051 889
Cuts at: 4424 6053 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1051 889 654 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2619 2813 3033 3051 3155 4018 1353
Size: 1167 99 194 220 18 104 863 3423
Fragments arranged by size:
3423 1167 863 220 194 104 99 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2624 4675 5049 5195 387
Size: 1064 138 1035 2051 374 146 1280
Fragments arranged by size:
2051 1280 1064 1035 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2153 2210 2486 2597 2819 2843
Size: 393 479 42 57 276 111 222 24
Cuts at: 2843 2966 3170 3288 3318 4151 4748 5535 5803
Size: 123 204 118 30 833 597 787 268
Cuts at: 5803 1239
Size: 1524
Fragments arranged by size:
1524 833 787 597 479 393 276 268
222 204 123 118 111 57 42 30
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 3110 2171
Size: 939 5149
EcoO109I rG'GnC_Cy
Cuts at: 2019 2494 3235 2019
Size: 475 741 4872
Fragments arranged by size:
4872 741 475
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
EcoRV GAT'ATC
Cuts at: 2764 2764
Size: 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 6088
FspI TGC'GCA
Cuts at: 2888 4594 2888
Size: 1706 4382
HaeII r_GCGC'y
Cuts at: 1678 2105 2539 2638 3052 3106 5235 1678
Size: 427 434 99 414 54 2129 2531
Fragments arranged by size:
2531 2129 434 427 414 99 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 3109 3141 4729
Size: 32 135 32 547 32 958 32 1588
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1588 958 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3432 3678 669
Size: 988 281 724 770 246 3079
Fragments arranged by size:
3079 988 770 724 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KasI G'GCGC_C
Cuts at: 2101 2101
Size: 6088
KpnI G_GTAC'C
Cuts at: 2167 3413 2167
Size: 1246 4842
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 3199 3504 4181 5329 5574 1606
Size: 441 1152 305 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 677 441 305 245
NaeI GCC'GGC
Cuts at: 2639 2639
Size: 6088
NarI GG'CG_CC
Cuts at: 2102 2102
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2637 2637
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2863 2893 2903 3446 4991 1132
Size: 97 1634 30 10 543 1545 2229
Fragments arranged by size:
2229 1634 1545 543 97 30 10
PasI CC'CwG_GG
Cuts at: 1348 3139 1348
Size: 1791 4297
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3385 1108
Size: 2277 3811
PfoI T'CCnGG_A
Cuts at: 2169 2781 2169
Size: 612 5476
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2494
Size: 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2223 3174 3440 830
Size: 1393 951 266 3478
Fragments arranged by size:
3478 1393 951 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2619 3033 2619
Size: 414 5674
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2219 3170 3436 4571 5252 5443 826
Size: 219 1174 951 266 1135 681 191 1471
Fragments arranged by size:
1471 1174 1135 951 681 266 219 191
SfoI GGC'GCC
Cuts at: 2103 2103
Size: 6088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 2492 3074 3415 1872
Size: 620 582 341 4545
Fragments arranged by size:
4545 620 582 341
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2348 2549 3263 3350 4192
Size: 197 926 369 36 201 714 87 842
Cuts at: 4192 5093 5355 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 842 714 369 262 201
197 87 36
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 2893 3446 1132
Size: 1761 553 3774
Fragments arranged by size:
3774 1761 553
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 2148 1517
Size: 631 5457
StyI C'CwwG_G
Cuts at: 513 1065 2814 3093 3527 513
Size: 552 1749 279 434 3074
Fragments arranged by size:
3074 1749 552 434 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3288 4115 4132 4285 4470 4915 325
Size: 1255 1708 827 17 153 185 445 1498
Fragments arranged by size:
1708 1498 1255 827 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 2185 2383
Size: 274 80 33 51 1443 174 34 198
Cuts at: 2383 4334 6046 96
Size: 1951 1712 138
Fragments arranged by size:
1951 1712 1443 274 198 174 138 80
51 34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 1316
Size: 960 5128
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2386
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 3350 1943
Size: 1407 4681
XmaI C'CCGG_G
Cuts at: 1870 2490 3072 3413 1870
Size: 620 582 341 4545
Fragments arranged by size:
4545 620 582 341
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI
SwaI