Restriction map for
pFN21AB5859
(Circular) (Six-base) MAPSORT of: pFN21AB5859.seq Check: 1969 from: 1 to: 6085
pFN21AB5859 6085 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2694 3261 4148 5532 5800 1632
Size: 1062 567 887 1384 268 1917
Fragments arranged by size:
1917 1384 1062 887 567 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 2492 4984 5961 1203
Size: 1289 2492 977 1327
Fragments arranged by size:
2492 1327 1289 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 2630 1166
Size: 1464 4621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 2315 3364 3383 3828 5400 2315
Size: 1049 19 445 1572 3000
Fragments arranged by size:
3000 1572 1049 445 19
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2279 3394 3620 3772 5881 1493
Size: 786 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 786 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 3410 1870
Size: 73 1467 4545
Fragments arranged by size:
4545 1467 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 3070 3406 4861 618
Size: 325 217 627 441 842 336 1455 1842
Fragments arranged by size:
1842 1455 842 627 441 336 325 217
BanII G_rGCy'C
Cuts at: 1391 3404 1391
Size: 2013 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 4938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 2231 4146 5302 1790
Size: 441 1915 1156 2573
Fragments arranged by size:
2573 1915 1156 441
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 3816 4774
Size: 416 53 83 399 24 816 1583 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1753 1583 958 816 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2792
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4745 1239
Size: 3506 2579
Bpu10I CC'TnA_GC
Cuts at: 2880 2880
Size: 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2073 2440 2743 4210 5075 5373 2073
Size: 367 303 1467 865 298 2785
Fragments arranged by size:
2785 1467 865 367 303 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3328 4748 882
Size: 617 1829 1420 2219
Fragments arranged by size:
2219 1829 1420 617
BsaAI yAC'GTr
Cuts at: 493 1204 2617 3876 493
Size: 711 1413 1259 2702
Fragments arranged by size:
2702 1413 1259 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3892 4274 4909 275
Size: 53 83 186 936 2359 382 635 1451
Fragments arranged by size:
2359 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5860
Fragments arranged by size:
5860 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 1186
Size: 1257 4828
BseYI C'CCAG_C
Cuts at: 5288 5784 5288
Size: 496 5589
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2438 2907 1163
Size: 573 702 469 4341
Fragments arranged by size:
4341 702 573 469
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4146 4231 5302 4146
Size: 85 1071 4929
Fragments arranged by size:
4929 1071 85
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 3404 4146 4231 5302 1391
Size: 399 441 1173 742 85 1071 2174
Fragments arranged by size:
2174 1173 1071 742 441 399 85
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3033 3974 5704 3033
Size: 941 1730 3414
Fragments arranged by size:
3414 1730 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 2810 4580 4754 65
Size: 1183 1413 149 1770 174 1396
Fragments arranged by size:
1770 1413 1396 1183 174 149
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstXI CCAn_nnnn'nTGG
Cuts at: 2856 2856
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2260 3415 3782 4167 4184 5636 5722 5734
Size: 288 1155 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 288 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 3601 4395 4415 2643
Size: 958 794 20 4313
Fragments arranged by size:
4313 958 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3262 3297 529
Size: 35 2698 35 3317
Fragments arranged by size:
3317 2698 35 35
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050
Size: 54 1425 50 275 18 1702 889 1629
Cuts at: 6050 8
Size: 43
Fragments arranged by size:
1702 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4015 1353
Size: 2662 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2694 3261 4148 4745 5532 5800 1239
Size: 393 1062 567 887 597 787 268 1524
Fragments arranged by size:
1524 1062 887 787 597 567 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2091 2091
Size: 6085
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 2220 2198
Size: 22 6063
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2810 2842 4726 4758 4800
Size: 32 135 32 1238 32 1884 32 42
Cuts at: 4800 4832 1373
Size: 32 2626
Fragments arranged by size:
2626 1884 1238 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 2726 3429 3675 669
Size: 988 281 724 64 703 246 3079
Fragments arranged by size:
3079 988 724 703 281 246 64
HindIII A'AGCT_T
Cuts at: 748 2645 2967 3078 748
Size: 1897 322 111 3755
Fragments arranged by size:
3755 1897 322 111
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3231 3684 3231
Size: 453 5632
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2032 5199 5383 1305
Size: 727 3167 184 2007
Fragments arranged by size:
3167 2007 727 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 4043 4402 4561 518
Size: 648 1464 1413 359 159 2042
Fragments arranged by size:
2042 1464 1413 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3161 3361 3501 4178 5326 5571 1606
Size: 1555 200 140 677 1148 245 2120
Fragments arranged by size:
2120 1555 1148 677 245 200 140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5533
NdeI CA'TA_TG
Cuts at: 387 2032 387
Size: 1645 4440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2800 3443 4988 1132
Size: 97 1571 643 1545 2229
Fragments arranged by size:
2229 1571 1545 643 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PstI C_TGCA'G
Cuts at: 830 3437 830
Size: 2607 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 3161 3361 3161
Size: 200 5885
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2112 3433 4568 5249 5440 826
Size: 219 1067 1321 1135 681 191 1471
Fragments arranged by size:
1471 1321 1135 1067 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2088 2455 2758 4189 5090 5352
Size: 197 926 145 367 303 1431 901 262
Cuts at: 5352 820
Size: 1553
Fragments arranged by size:
1553 1431 926 901 367 303 262 197
145
SnaBI TAC'GTA
Cuts at: 493 2617 3876 493
Size: 2124 1259 2702
Fragments arranged by size:
2702 2124 1259
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 3524 513
Size: 552 2459 3074
Fragments arranged by size:
3074 2459 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3115 4112 4129 4282 4467 4912 325
Size: 1255 1535 997 17 153 185 445 1498
Fragments arranged by size:
1535 1498 1255 997 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3291 4331 6043
Size: 274 80 33 51 1443 1314 1040 1712
Cuts at: 6043 96
Size: 138
Fragments arranged by size:
1712 1443 1314 1040 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2853 2853
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI