Restriction map for
pFN21AB5846
(Circular) (Six-base) MAPSORT of: pFN21AB5846.seq Check: 1436 from: 1 to: 6160
pFN21AB5846 6160 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 2888 3481 2888
Size: 593 5567
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 3382 4223 5607 5875 1632
Size: 626 1124 841 1384 268 1917
Fragments arranged by size:
1917 1384 1124 841 626 268
AflII C'TTAA_G
Cuts at: 820 1017 2132 820
Size: 197 1115 4848
Fragments arranged by size:
4848 1115 197
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 2523
Size: 32 6128
AlwNI CAG_nnn'CTG
Cuts at: 2236 2269 2296 3903 5475 2236
Size: 33 27 1607 1572 2921
Fragments arranged by size:
2921 1607 1572 33 27
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 2087 2595 2858 3180 3264 3348 3432 3469
Size: 594 508 263 322 84 84 84 37
Cuts at: 3469 3695 3847 5956 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 594 508 322 263 226 152
84 84 84 37
AseI AT'TA_AT
Cuts at: 160 1032 3455 4714 160
Size: 872 2423 1259 1606
Fragments arranged by size:
2423 1606 1259 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 3485 1870
Size: 73 1542 4545
Fragments arranged by size:
4545 1542 73
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2888 3481 4936 618
Size: 325 217 627 1101 593 1455 1842
Fragments arranged by size:
1842 1455 1101 627 593 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2223 2906 2990 3158 3326 3410 3479
Size: 754 78 683 84 168 168 84 69
Cuts at: 3479 1391
Size: 4072
Fragments arranged by size:
4072 754 683 168 168 84 84 78
69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2639 4052 5262 2639
Size: 1413 1210 3537
Fragments arranged by size:
3537 1413 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4771 136
Size: 107 122 71 631 605 3099 1525
Fragments arranged by size:
3099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3160 6155 3160
Size: 2995 3165
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 4221 5377 1790
Size: 2431 1156 2573
Fragments arranged by size:
2573 2431 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 3891 4849
Size: 416 53 83 399 24 1781 693 958
Cuts at: 4849 442
Size: 1753
Fragments arranged by size:
1781 1753 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2028 2396 2970 3126 3378 4820 1239
Size: 789 368 574 156 252 1442 2579
Fragments arranged by size:
2579 1442 789 574 368 252 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2658 4285 5150 5448 2658
Size: 1627 865 298 3370
Fragments arranged by size:
3370 1627 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2498 4823 882
Size: 617 999 2325 2219
Fragments arranged by size:
2325 2219 999 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 2650 6154 2650
Size: 3504 2656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3967 4349 4984 275
Size: 53 83 186 936 2434 382 635 1451
Fragments arranged by size:
2434 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2523 2553 2888 2918 1178
Size: 30 165 30 1120 30 335 30 4420
Fragments arranged by size:
4420 1120 335 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2848 1186
Size: 1186 476 4498
Fragments arranged by size:
4498 1186 476
BseYI C'CCAG_C
Cuts at: 2535 5363 5859 2535
Size: 2828 496 2836
Fragments arranged by size:
2836 2828 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5587
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2223 2906 3158 3326 3410 4221 4306 5377
Size: 78 683 252 168 84 811 85 1071
Cuts at: 5377 2145
Size: 2928
Fragments arranged by size:
2928 1071 811 683 252 168 85 84
78
BsmI GAATG_Cn'
Cuts at: 3227 3380 3671 3764 3227
Size: 153 291 93 5623
Fragments arranged by size:
5623 291 153 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2223 2906 2990 3158 3326 3410
Size: 399 355 78 683 84 168 168 84
Cuts at: 3410 3479 4221 4306 5377 1391
Size: 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 399 355 168 168
85 84 84 78 69
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3515 844
Size: 2671 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4804 4991 1700
Size: 110 2994 187 2869
Fragments arranged by size:
2994 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstXI CCAn_nnnn'nTGG
Cuts at: 2432 2432
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2247 2576 3160 3490 3857 4242 4259 5711
Size: 275 329 584 330 367 385 17 1452
Cuts at: 5711 5797 5809 6155 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 584 385 367 346 330 329
275 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2095 513
Size: 552 939 91 4578
Fragments arranged by size:
4578 939 552 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 3385 3676 4470 4490 2370
Size: 1015 291 794 20 4040
Fragments arranged by size:
4040 1015 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3199 3234 529
Size: 35 2635 35 3455
Fragments arranged by size:
3455 2635 35 35
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3607 4496 6125
Size: 54 1425 50 275 18 1777 889 1629
Cuts at: 6125 8
Size: 43
Fragments arranged by size:
1777 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3385 4090 1353
Size: 2032 705 3423
Fragments arranged by size:
3423 2032 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 2221 2904 3156 3324 3408 2143
Size: 78 683 252 168 84 4895
Fragments arranged by size:
4895 683 252 168 84 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2258 2396 2970 3126 3378 3382
Size: 393 396 230 138 574 156 252 4
Cuts at: 3382 4223 4820 5607 5875 1239
Size: 841 597 787 268 1524
Fragments arranged by size:
1524 841 787 597 574 396 393 268
252 230 156 138 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 6160
EcoRI G'AATT_C
Cuts at: 2858 3180 3264 3348 3432 3469 3847 2858
Size: 322 84 84 84 37 378 5171
Fragments arranged by size:
5171 378 322 84 84 84 37
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 5307 1678
Size: 3629 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2827 2859 2888 2920 3145
Size: 32 135 32 1255 32 29 32 225
Cuts at: 3145 3177 3313 3345 4801 4833 4875 4907 1373
Size: 32 136 32 1456 32 42 32 2626
Fragments arranged by size:
2626 1456 1255 225 136 135 42 32
32 32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3422 3504 3750 669
Size: 988 281 1484 82 246 3079
Fragments arranged by size:
3079 1484 988 281 246 82
HindIII A'AGCT_T
Cuts at: 748 3057 3142 748
Size: 2309 85 3766
Fragments arranged by size:
3766 2309 85
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 2892 3485 2892
Size: 593 5567
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 2967 4118 4477 4636 518
Size: 648 1264 537 1151 359 159 2042
Fragments arranged by size:
2042 1264 1151 648 537 359 159
MspA1I CmG'CkG
Cuts at: 1606 3576 4253 5401 5646 1606
Size: 1970 677 1148 245 2120
Fragments arranged by size:
2120 1970 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2095 513
Size: 552 939 91 4578
Fragments arranged by size:
4578 939 552 91
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NruI TCG'CGA
Cuts at: 2196 2196
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 3008 3518 5063 1132
Size: 97 1779 510 1545 2229
Fragments arranged by size:
2229 1779 1545 510 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2351 1108
Size: 1243 4917
PfoI T'CCnGG_A
Cuts at: 2185 2214 2467 2185
Size: 29 253 5878
Fragments arranged by size:
5878 253 29
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2555 4229 4261 5742 5774 2523
Size: 32 1674 32 1481 32 2909
Fragments arranged by size:
2909 1674 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2746 1613
Size: 1133 5027
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2400 2432 2400
Size: 32 6128
PstI C_TGCA'G
Cuts at: 830 2032 2150 2695 3512 830
Size: 1202 118 545 817 3478
Fragments arranged by size:
3478 1202 817 545 118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
SacI G_AGCT'C
Cuts at: 2145 2223 2906 3158 3326 3410 2145
Size: 78 683 252 168 84 4895
Fragments arranged by size:
4895 683 252 168 84 78
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SapI GCTCTTCn'nnn_
Cuts at: 3385 3385
Size: 6160
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 2409 4408 6120 2409
Size: 1999 1712 2449
Fragments arranged by size:
2449 1999 1712
SfcI C'TryA_G
Cuts at: 826 1045 2010 2028 2146 2691 3508 4643 5324
Size: 219 965 18 118 545 817 1135 681
Cuts at: 5324 5515 826
Size: 191 1471
Fragments arranged by size:
1471 1135 965 817 681 545 219 191
118 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2673 4264 5165 5427 820
Size: 197 926 189 541 1591 901 262 1553
Fragments arranged by size:
1591 1553 926 901 541 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 2445 4084 5
Size: 47 2393 1639 2081
Fragments arranged by size:
2393 2081 1639 47
StuI AGG'CCT
Cuts at: 1517 2324 1517
Size: 807 5353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2256 2508 3599 513
Size: 552 939 91 161 252 1091 3074
Fragments arranged by size:
3074 1091 939 552 252 161 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2798 4187 4204 4357 4542 4987 325
Size: 1255 1218 1389 17 153 185 445 1498
Fragments arranged by size:
1498 1389 1255 1218 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2407 4406 6118
Size: 274 80 33 51 1443 430 1999 1712
Cuts at: 6118 96
Size: 138
Fragments arranged by size:
1999 1712 1443 430 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2587 2183
Size: 404 5756
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2058 2265 3059 3311 4289 2058
Size: 207 794 252 978 3929
Fragments arranged by size:
3929 978 794 252 207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI