Restriction map for
pFN21AB5840
(Circular) (Six-base) MAPSORT of: pFN21AB5840.seq Check: 2110 from: 1 to: 6151
pFN21AB5840 6151 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 3356 2192
Size: 1164 4987
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 3414 4214 5598 5866 1632
Size: 954 828 800 1384 268 1917
Fragments arranged by size:
1917 1384 954 828 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 2715 5050 6027 1203
Size: 1512 2335 977 1327
Fragments arranged by size:
2335 1512 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 2734 3392 3894 5466 2734
Size: 658 502 1572 3419
Fragments arranged by size:
3419 1572 658 502
ApaI G_GGCC'C
Cuts at: 3244 3244
Size: 6151
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2461 3460 3686 3838 5947 1493
Size: 968 999 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 968 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 2904 3481 3848 2904
Size: 577 367 5207
Fragments arranged by size:
5207 577 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2585 2811 3244 3470 1391
Size: 1194 226 433 226 4072
Fragments arranged by size:
4072 1194 433 226 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BfrBI ATG'CAT
Cuts at: 2200 2200
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 2964 3568 2964
Size: 604 5547
Bme1580I G_kGCm'C
Cuts at: 1790 2648 3244 4212 5368 1790
Size: 858 596 968 1156 2573
Fragments arranged by size:
2573 1156 968 858 596
BmgBI CAC'GTC
Cuts at: 1318 3134 1318
Size: 1816 4335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2509 2880 3052
Size: 416 53 83 399 24 1092 371 172
Cuts at: 3052 3882 4840 442
Size: 830 958 1753
Fragments arranged by size:
1753 1092 958 830 416 399 371 172
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4811 1239
Size: 3572 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2383 2921 4276 5141 5439 2383
Size: 538 1355 865 298 3095
Fragments arranged by size:
3095 1355 865 538 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2301 4814 882
Size: 617 802 2513 2219
Fragments arranged by size:
2513 2219 802 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5926
Fragments arranged by size:
5926 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2971 3065 3212 1186
Size: 1785 94 147 4125
Fragments arranged by size:
4125 1785 147 94
BseYI C'CCAG_C
Cuts at: 3334 5354 5850 3334
Size: 2020 496 3635
Fragments arranged by size:
3635 2020 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2545 2843 3133 1163
Size: 573 809 298 290 4181
Fragments arranged by size:
4181 809 573 298 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 4212 4297 5368 2811
Size: 1401 85 1071 3594
Fragments arranged by size:
3594 1401 1071 85
BsmI GAATG_Cn'
Cuts at: 2512 2943 3008 3662 3755 2512
Size: 431 65 654 93 4908
Fragments arranged by size:
4908 654 431 93 65
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2585 2648 2811 3244 3470 4212 4297
Size: 399 795 63 163 433 226 742 85
Cuts at: 4297 5368 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 795 742 433 399 226 163
85 63
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 3415 4040 5770 3415
Size: 625 1730 3796
Fragments arranged by size:
3796 1730 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2192 2477 3105 3356 3506 844
Size: 1334 14 285 628 251 150 3489
Fragments arranged by size:
3489 1334 628 285 251 150 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 4038 1394
Size: 429 234 1981 3507
Fragments arranged by size:
3507 1981 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 3102 4646 4820 65
Size: 1183 1854 1544 174 1396
Fragments arranged by size:
1854 1544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2734 794
Size: 1940 4211
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 2179 1184
Size: 995 5156
BstYI r'GATC_y
Cuts at: 1972 2850 2904 3445 3481 3848 4233 4250 5702
Size: 878 54 541 36 367 385 17 1452
Cuts at: 5702 5788 5800 6146 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 878 541 385 367 346 86
54 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2622 2698 513
Size: 552 939 618 76 3966
Fragments arranged by size:
3966 939 618 552 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3667 4461 4481 2664
Size: 1003 794 20 4334
Fragments arranged by size:
4334 1003 794 20
ClaI AT'CG_AT
Cuts at: 1554 2411 1554
Size: 857 5294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2542 3187 3598
Size: 54 1425 50 275 18 712 645 411
Cuts at: 3598 4487 6116 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 712 645 411 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3054 3309 4081 1353
Size: 1701 255 772 3423
Fragments arranged by size:
3423 1701 772 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2145 4738 5112 5258 387
Size: 1064 138 435 121 2593 374 146 1280
Fragments arranged by size:
2593 1280 1064 435 374 146 138 121
EcoICRI GAG'CTC
Cuts at: 2809 2809
Size: 6151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2586 3414 4214 4811 5598 5866 1239
Size: 393 954 828 800 597 787 268 1524
Fragments arranged by size:
1524 954 828 800 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2896 5298 1678
Size: 1218 2402 2531
Fragments arranged by size:
2531 2402 1218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4792 4824 4866 4898 1373
Size: 32 135 32 3220 32 42 32 2626
Fragments arranged by size:
3220 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2398 2937 5265 5449 1305
Size: 1093 539 2328 184 2007
Fragments arranged by size:
2328 2007 1093 539 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 4109 4468 4627 518
Size: 648 1695 1248 359 159 2042
Fragments arranged by size:
2042 1695 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2157 2313 2641 3567 4244 5392 5637
Size: 457 94 156 328 926 677 1148 245
Cuts at: 5637 1606
Size: 2120
Fragments arranged by size:
2120 1148 926 677 457 328 245 156
94
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NsiI A_TGCA'T
Cuts at: 2202 2202
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2719 3176 3509 5054 1132
Size: 97 1490 457 333 1545 2229
Fragments arranged by size:
2229 1545 1490 457 333 97
PasI CC'CwG_GG
Cuts at: 1348 2119 1348
Size: 771 5380
PciI A'CATG_T
Cuts at: 2715 5050 2715
Size: 2335 3816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PfoI T'CCnGG_A
Cuts at: 2023 2023
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2226 1613
Size: 613 5538
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PspOMI G'GGCC_C
Cuts at: 3240 3240
Size: 6151
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 6119
PstI C_TGCA'G
Cuts at: 830 2189 2318 2580 2659 2730 3367 3503 830
Size: 1359 129 262 79 71 637 136 3478
Fragments arranged by size:
3478 1359 637 262 136 129 79 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2641 2641
Size: 6151
SacI G_AGCT'C
Cuts at: 2811 2811
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 2189 2580 3503 2189
Size: 391 923 4837
Fragments arranged by size:
4837 923 391
ScaI AGT'ACT
Cuts at: 3041 4399 6111 3041
Size: 1358 1712 3081
Fragments arranged by size:
3081 1712 1358
SfcI C'TryA_G
Cuts at: 826 1045 2185 2314 2361 2478 2576 2655 2726
Size: 219 1140 129 47 117 98 79 71
Cuts at: 2726 3363 3499 4634 5315 5506 826
Size: 637 136 1135 681 191 1471
Fragments arranged by size:
1471 1140 1135 681 637 219 191 136
129 117 98 79 71 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2398 2936 4255 5156 5418 820
Size: 197 926 455 538 1319 901 262 1553
Fragments arranged by size:
1553 1319 926 901 538 455 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3176 3509 1132
Size: 2044 333 3774
Fragments arranged by size:
3774 2044 333
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3590 513
Size: 552 939 588 998 3074
Fragments arranged by size:
3074 998 939 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 3034 4178 4195 4348 4533 4978
Size: 1255 911 543 1144 17 153 185 445
Cuts at: 4978 325
Size: 1498
Fragments arranged by size:
1498 1255 1144 911 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3039 4397 6109
Size: 274 80 33 51 1443 1062 1358 1712
Cuts at: 6109 96
Size: 138
Fragments arranged by size:
1712 1443 1358 1062 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 6111 6143 2828
Size: 32 3251 32 2836
Fragments arranged by size:
3251 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3039 3039
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 3257 4280 3257
Size: 1023 5128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI