Restriction map for
pFN21AB5838
(Circular) (Six-base) MAPSORT of: pFN21AB5838.seq Check: 901 from: 1 to: 6148
pFN21AB5838 6148 bp
With 182 enzymes: SgfI *
March 28, 2012 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3200 3200
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 3141 3491 1937
Size: 861 343 350 4594
Fragments arranged by size:
4594 861 350 343
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 2641 4275 4648 1563
Size: 1078 1634 373 3063
Fragments arranged by size:
3063 1634 1078 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3447 4211 5595 5863 1632
Size: 1815 764 1384 268 1917
Fragments arranged by size:
1917 1815 1384 764 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 2624 1166
Size: 1458 4690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2460 1184
Size: 1276 4872
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3300 3457 3683 3835 5944 1493
Size: 1807 157 226 152 2109 1697
Fragments arranged by size:
2109 1807 1697 226 157 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3473 1870
Size: 73 1530 4545
Fragments arranged by size:
4545 1530 73
AvrII C'CTAG_G
Cuts at: 2980 2980
Size: 6148
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3467 1391
Size: 2076 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3360 928
Size: 971 1461 3716
Fragments arranged by size:
3716 1461 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BfrBI ATG'CAT
Cuts at: 2437 3173 2437
Size: 736 5412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3246 6143 3246
Size: 2897 3251
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3879 4837
Size: 416 53 83 399 24 762 1700 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1700 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 3110 3223 4808 1239
Size: 1028 843 113 1585 2579
Fragments arranged by size:
2579 1585 1028 843 113
Bpu10I CC'TnA_GC
Cuts at: 2049 2049
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2836 3255 4581 4979 5253 5400 5811 2836
Size: 419 1326 398 274 147 411 3173
Fragments arranged by size:
3173 1326 419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3389 1186
Size: 2203 3945
BseYI C'CCAG_C
Cuts at: 5351 5847 5351
Size: 496 5652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2963 1163
Size: 573 414 813 4348
Fragments arranged by size:
4348 813 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 2069 4209 4294 5365 2055
Size: 14 2140 85 1071 2838
Fragments arranged by size:
2838 2140 1071 85 14
BsmI GAATG_Cn'
Cuts at: 2519 3659 3752 2519
Size: 1140 93 4915
Fragments arranged by size:
4915 1140 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2069 3467 4209 4294 5365 1391
Size: 399 265 14 1398 742 85 1071 2174
Fragments arranged by size:
2174 1398 1071 742 399 265 85 14
BspEI T'CCGG_A
Cuts at: 3255 5811 3255
Size: 2556 3592
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3200 3503 844
Size: 2356 303 3489
Fragments arranged by size:
3489 2356 303
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2559 2856 4643 4817 65
Size: 1183 1311 297 1787 174 1396
Fragments arranged by size:
1787 1396 1311 1183 297 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 794
Size: 1265 4883
BstBI TT'CG_AA
Cuts at: 3304 4952 3304
Size: 1648 4500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2455
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 3246 3478 3845 4230 4247 5699 5785 5797
Size: 1274 232 367 385 17 1452 86 12
Cuts at: 5797 6143 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1274 385 367 346 232 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3121 3121
Size: 6148
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3664 4458 4478 3664
Size: 794 20 5334
Fragments arranged by size:
5334 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 3595 4484
Size: 54 1425 50 275 18 1348 417 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1348 889 417 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2184 3155 3423 4078 1353
Size: 739 92 971 268 655 3423
Fragments arranged by size:
3423 971 739 655 268 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 3110 3223 3447 4211 4808 5595
Size: 393 635 843 113 224 764 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1524 843 787 764 635 597 393 268
224 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 2580
Size: 6148
EcoO109I rG'GnC_Cy
Cuts at: 2977 2977
Size: 6148
EcoRI G'AATT_C
Cuts at: 3300 3457 3835 3300
Size: 157 378 5613
Fragments arranged by size:
5613 378 157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 3268 2767
Size: 501 5647
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4789 4821 4863 4895 1373
Size: 32 135 32 3217 32 42 32 2626
Fragments arranged by size:
3217 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3492 3738 669
Size: 988 281 852 702 246 3079
Fragments arranged by size:
3079 988 852 702 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 2208 2331 2925 3747 2208
Size: 123 594 822 4609
Fragments arranged by size:
4609 822 594 123
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 2979 5262 5446 1305
Size: 988 686 2283 184 2007
Fragments arranged by size:
2283 2007 988 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 2453 2624 4106 4465 4624 518
Size: 648 1276 11 171 1482 359 159 2042
Fragments arranged by size:
2042 1482 1276 648 359 171 159 11
MspA1I CmG'CkG
Cuts at: 1606 3564 4241 5389 5634 1606
Size: 1958 677 1148 245 2120
Fragments arranged by size:
2120 1958 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2439 3175 2439
Size: 736 5412
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PfoI T'CCnGG_A
Cuts at: 3087 3087
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2977 2977
Size: 6148
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2262 3500 830
Size: 1432 1238 3478
Fragments arranged by size:
3478 1432 1238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2184 3423 2184
Size: 1239 4909
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2258 2514 2799 3496 4631 5312 5503
Size: 219 1213 256 285 697 1135 681 191
Cuts at: 5503 826
Size: 1471
Fragments arranged by size:
1471 1213 1135 697 681 285 256 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2980 3587 513
Size: 552 939 285 691 607 3074
Fragments arranged by size:
3074 939 691 607 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4175 4192 4345 4530 4975 325
Size: 1255 2595 17 153 185 445 1498
Fragments arranged by size:
2595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 6108 6140 2307
Size: 32 3769 32 2315
Fragments arranged by size:
3769 2315 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2794 1316
Size: 1478 4670
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI