Restriction map for
pFN21AB5836
(Circular) (Six-base) MAPSORT of: pFN21AB5836.seq Check: 6972 from: 1 to: 6148
pFN21AB5836 6148 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2024 3958 278
Size: 53 83 186 936 488 1934 2468
Fragments arranged by size:
2468 1934 936 488 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 4211 5595 5863 1632
Size: 738 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 738 268
AflII C'TTAA_G
Cuts at: 820 1017 2296 820
Size: 197 1279 4672
Fragments arranged by size:
4672 1279 197
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 3021 1166
Size: 1855 4293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2404 2530 2698 2925 3083 3457 3683 3835
Size: 911 126 168 227 158 374 226 152
Cuts at: 3835 5944 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 911 374 227 226 168 158
152 126
AseI AT'TA_AT
Cuts at: 160 1032 2286 4702 160
Size: 872 1254 2416 1606
Fragments arranged by size:
2416 1606 1254 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3473 1870
Size: 73 1530 4545
Fragments arranged by size:
4545 1530 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3064 3467 1391
Size: 1673 403 4072
Fragments arranged by size:
4072 1673 403
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BbvCI CC'TCA_GC
Cuts at: 2049 2049
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4040 5250 3209
Size: 831 1210 4107
Fragments arranged by size:
4107 1210 831
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2821 6143 2821
Size: 3322 2826
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3879 4837 442
Size: 416 53 83 399 24 2462 958 1753
Fragments arranged by size:
2462 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 4808 1239
Size: 921 2648 2579
Fragments arranged by size:
2648 2579 921
Bpu10I CC'TnA_GC
Cuts at: 2049 2049
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 4273 5138 5436 2230
Size: 2043 865 298 2942
Fragments arranged by size:
2942 2043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3955 4337 4972
Size: 53 83 186 936 488 1934 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1934 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6148
BseYI C'CCAG_C
Cuts at: 5351 5847 5351
Size: 496 5652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4209 4294 5365 4209
Size: 85 1071 4992
Fragments arranged by size:
4992 1071 85
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3064 3467 4209 4294 5365 1391
Size: 399 1274 403 742 85 1071 2174
Fragments arranged by size:
2174 1274 1071 742 403 399 85
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3503 844
Size: 2659 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2576 4035 1394
Size: 429 753 1459 3507
Fragments arranged by size:
3507 1459 753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 2656 1184
Size: 1472 4676
BstXI CCAn_nnnn'nTGG
Cuts at: 2973 2973
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2188 2821 3478 3845 4230 4247 5699 5785
Size: 216 633 657 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 657 633 385 367 346 216
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4516
Fragments arranged by size:
4516 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3084 3664 4458 4478 3084
Size: 580 794 20 4754
Fragments arranged by size:
4754 794 580 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 2535 3452 2535
Size: 917 5231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2937 3595
Size: 54 1425 50 275 18 276 831 658
Cuts at: 3595 4484 6113 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 831 658 276 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 3192 4078 1353
Size: 993 846 886 3423
Fragments arranged by size:
3423 993 886 846
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2370 4211 4808 5595 5863 1239
Size: 393 528 210 1841 597 787 268 1524
Fragments arranged by size:
1841 1524 787 597 528 393 268 210
EcoNI CCTnn'n_nnAGG
Cuts at: 2784 2784
Size: 6148
EcoRI G'AATT_C
Cuts at: 2404 3083 3457 3835 2404
Size: 679 374 378 4717
Fragments arranged by size:
4717 679 378 374
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 3240 5295 1678
Size: 1562 2055 2531
Fragments arranged by size:
2531 2055 1562
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3207 3239 4789 4821 4863
Size: 32 135 32 1635 32 1550 32 42
Cuts at: 4863 4895 1373
Size: 32 2626
Fragments arranged by size:
2626 1635 1550 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3492 3738 669
Size: 988 281 1467 87 246 3079
Fragments arranged by size:
3079 1467 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3405 3738 3405
Size: 333 5815
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 5262 5446 1305
Size: 1035 2922 184 2007
Fragments arranged by size:
2922 2007 1035 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 10
Size: 54 1425 343 1107 3219
Fragments arranged by size:
3219 1425 1107 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2805 2833 3021 3207 4106 4465
Size: 648 1479 160 28 188 186 899 359
Cuts at: 4465 4624 518
Size: 159 2042
Fragments arranged by size:
2042 1479 899 648 359 188 186 160
159 28
MspA1I CmG'CkG
Cuts at: 1606 2048 3564 4241 5389 5634 1606
Size: 442 1516 677 1148 245 2120
Fragments arranged by size:
2120 1516 1148 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4516
Fragments arranged by size:
4516 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2373 2373
Size: 6148
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3315 1613
Size: 1702 4446
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2096 3500 830
Size: 1266 1404 3478
Fragments arranged by size:
3478 1404 1266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2048 2048
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2346 3192 2346
Size: 846 5302
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 3365 4396 6108 3365
Size: 1031 1712 3405
Fragments arranged by size:
3405 1712 1031
SfcI C'TryA_G
Cuts at: 826 1045 2092 3496 4631 5312 5503 826
Size: 219 1047 1404 1135 681 191 1471
Fragments arranged by size:
1471 1404 1135 1047 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 2296 4252 5153 5415 820
Size: 197 926 302 51 1956 901 262 1553
Fragments arranged by size:
1956 1553 926 901 302 262 197 51
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 3053 4072 5
Size: 47 3001 1019 2081
Fragments arranged by size:
3001 2081 1019 47
StuI AGG'CCT
Cuts at: 1517 3069 1517
Size: 1552 4596
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2940 3070 3313 3587 513
Size: 552 939 141 795 130 243 274 3074
Fragments arranged by size:
3074 939 795 552 274 243 141 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4175 4192 4345 4530 4975 325
Size: 1255 2595 17 153 185 445 1498
Fragments arranged by size:
2595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3363 4394 6106
Size: 274 80 33 51 1443 1386 1031 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1712 1443 1386 1031 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2087 2631 2715 3344 4277 2087
Size: 544 84 629 933 3958
Fragments arranged by size:
3958 933 629 544 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2022 3956 276
Size: 53 83 186 936 488 1934 2468
Fragments arranged by size:
2468 1934 936 488 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI