Restriction map for
pFN21AB5832
(Circular) (Six-base) MAPSORT of: pFN21AB5832.seq Check: 7865 from: 1 to: 6148
pFN21AB5832 6148 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2219 2978 2219
Size: 759 5389
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 4211 5595 5863 1632
Size: 675 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 675 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 2174 2495 1166
Size: 1008 321 4819
Fragments arranged by size:
4819 1008 321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 2570 3891 5463 2570
Size: 1321 1572 3255
Fragments arranged by size:
3255 1572 1321
ApaI G_GGCC'C
Cuts at: 2030 2036 2466 3180 2030
Size: 6 430 714 4998
Fragments arranged by size:
4998 714 430 6
ApaLI G'TGCA_C
Cuts at: 2203 4205 5361 2203
Size: 2002 1156 2990
Fragments arranged by size:
2990 2002 1156
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2035 2581 2972 3473 1870
Size: 73 86 6 546 391 501 4545
Fragments arranged by size:
4545 546 501 391 86 73 6
BamHI G'GATC_C
Cuts at: 2383 3478 3845 2383
Size: 1095 367 4686
Fragments arranged by size:
4686 1095 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2722 2906 2920 2977
Size: 325 217 627 304 631 184 14 57
Cuts at: 2977 3169 3469 4924 618
Size: 192 300 1455 1842
Fragments arranged by size:
1842 1455 631 627 325 304 300 217
192 184 57 14
BanII G_rGCy'C
Cuts at: 1391 2030 2036 2466 2507 3180 3467 1391
Size: 639 6 430 41 673 287 4072
Fragments arranged by size:
4072 673 639 430 287 41 6
BbeI G_GCGC'C
Cuts at: 3173 3173
Size: 6148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2989 3023 4339 4373 1903
Size: 34 1052 34 1316 34 3678
Fragments arranged by size:
3678 1316 1052 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 4759 136
Size: 107 122 71 631 605 368 2719 1525
Fragments arranged by size:
2719 1525 631 605 368 122 107 71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2036 2207 2466 2982 3180 4209 5365
Size: 240 6 171 259 516 198 1029 1156
Cuts at: 5365 1790
Size: 2573
Fragments arranged by size:
2573 1156 1029 516 259 240 198 171
6
BmgBI CAC'GTC
Cuts at: 1318 2953 1318
Size: 1635 4513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3118 3243 3879
Size: 416 53 83 399 24 1701 125 636
Cuts at: 3879 4837 442
Size: 958 1753
Fragments arranged by size:
1753 1701 958 636 416 399 125 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2823 3020 4808 1239
Size: 1154 430 197 1788 2579
Fragments arranged by size:
2579 1788 1154 430 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2691 4811 882
Size: 617 1192 2120 2219
Fragments arranged by size:
2219 2120 1192 617
BsaAI yAC'GTr
Cuts at: 493 1204 2176 3939 493
Size: 711 972 1763 2702
Fragments arranged by size:
2702 1763 972 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3170 3955 4337 4972
Size: 53 83 186 936 1637 785 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1637 1451 936 785 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 5221
Fragments arranged by size:
5221 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 1186
Size: 1455 4693
BseYI C'CCAG_C
Cuts at: 2110 2157 2810 2865 3200 3256 3365 5351 5847
Size: 47 653 55 335 56 109 1986 496
Cuts at: 5847 2110
Size: 2411
Fragments arranged by size:
2411 1986 653 496 335 109 56 55
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2535 2694 1163
Size: 573 783 16 159 4617
Fragments arranged by size:
4617 783 573 159 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2207 2633 4209 4294 5365 2207
Size: 426 1576 85 1071 2990
Fragments arranged by size:
2990 1576 1071 426 85
BsmI GAATG_Cn'
Cuts at: 2738 3659 3752 2738
Size: 921 93 5134
Fragments arranged by size:
5134 921 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2562 2562
Size: 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2036 2207 2466 2507 2633 2982
Size: 399 240 6 171 259 41 126 349
Cuts at: 2982 3180 3467 4209 4294 5365 1391
Size: 198 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 287 259 240
198 171 126 85 41 6
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 3387 4037 5767 3387
Size: 650 1730 3768
Fragments arranged by size:
3768 1730 650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 2685 2978 3503 844
Size: 1375 466 293 525 3489
Fragments arranged by size:
3489 1375 525 466 293
BsrBI CCG'CTC
Cuts at: 1394 1823 3034 4035 1394
Size: 429 1211 1001 3507
Fragments arranged by size:
3507 1211 1001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2211 2744 794
Size: 1417 533 4198
Fragments arranged by size:
4198 1417 533
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 2167 1184
Size: 983 5165
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2917 3161 3166 3365 2011
Size: 906 244 5 199 4794
Fragments arranged by size:
4794 906 244 199 5
BstYI r'GATC_y
Cuts at: 1972 2383 3369 3478 3845 4230 4247 5699 5785
Size: 411 986 109 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 986 411 385 367 346 109
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2308 513
Size: 552 939 183 121 4353
Fragments arranged by size:
4353 939 552 183 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 2140 2488 2809 3202 3228 3664 4458 4478 2140
Size: 348 321 393 26 436 794 20 3810
Fragments arranged by size:
3810 794 436 393 348 321 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 3595 4484
Size: 54 1425 50 275 18 1189 576 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1189 889 576 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2821 3011 3313 4078 1353
Size: 1070 398 190 302 765 3423
Fragments arranged by size:
3423 1070 765 398 302 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 2084 4735 5109 5255 387
Size: 1064 138 489 6 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 489 374 146 138 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2393 2823 3020 4211 4808 5595
Size: 393 675 86 430 197 1191 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1524 1191 787 675 597 430 393 268
197 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2769 2769
Size: 6148
EcoO109I rG'GnC_Cy
Cuts at: 2026 2462 2969 3025 3176 3237 2026
Size: 436 507 56 151 61 4937
Fragments arranged by size:
4937 507 436 151 61 56
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
EcoRV GAT'ATC
Cuts at: 2182 2182
Size: 6148
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 3173 5295 1678
Size: 1495 2122 2531
Fragments arranged by size:
2531 2122 1495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2424 2456 3311 3343 4789
Size: 32 135 32 852 32 855 32 1446
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 855 852 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KasI G'GCGC_C
Cuts at: 3169 3169
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3452 5262 5446 1305
Size: 2147 1810 184 2007
Fragments arranged by size:
2147 2007 1810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3021 10
Size: 54 1425 343 1189 3137
Fragments arranged by size:
3137 1425 1189 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2174 2495 4106 4465 4624 518
Size: 648 843 165 321 1611 359 159 2042
Fragments arranged by size:
2042 1611 843 648 359 321 165 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2843 2984 3133 3265 3365 3564 4241
Size: 446 791 141 149 132 100 199 677
Cuts at: 4241 5389 5634 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 446 245 199 149
141 132 100
NarI GG'CG_CC
Cuts at: 3170 3170
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3233 1108
Size: 2125 4023
PfoI T'CCnGG_A
Cuts at: 2409 2409
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2969 3025 2969
Size: 56 6092
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2026 2032 2462 3176 2026
Size: 6 430 714 4998
Fragments arranged by size:
4998 714 430 6
PspXI vC'TCGA_Gb
Cuts at: 2581 2581
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2123 2881 2989 3500 830
Size: 1293 758 108 511 3478
Fragments arranged by size:
3478 1293 758 511 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2843 3365 2843
Size: 522 5626
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SanDI GG'GwC_CC
Cuts at: 2969 2969
Size: 6148
SapI GCTCTTCn'nnn_
Cuts at: 2821 3313 2821
Size: 492 5656
SbfI CC_TGCA'GG
Cuts at: 2881 3500 2881
Size: 619 5529
ScaI AGT'ACT
Cuts at: 2671 4396 6108 2671
Size: 1725 1712 2711
Fragments arranged by size:
2711 1725 1712
SfcI C'TryA_G
Cuts at: 826 1045 2119 2877 2985 3496 4631 5312 5503
Size: 219 1074 758 108 511 1135 681 191
Cuts at: 5503 826
Size: 1471
Fragments arranged by size:
1471 1135 1074 758 681 511 219 191
108
SfoI GGC'GCC
Cuts at: 3171 3171
Size: 6148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 2031 2037 2974 3475 1872
Size: 159 6 937 501 4545
Fragments arranged by size:
4545 937 501 159 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2581 4252 5153 5415 820
Size: 197 926 638 1671 901 262 1553
Fragments arranged by size:
1671 1553 926 901 638 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SrfI GCCC'GGGC
Cuts at: 2031 2037 2031
Size: 6 6142
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 2770 3587 513
Size: 552 939 766 817 3074
Fragments arranged by size:
3074 939 817 766 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2531 3244 4175 4192 4345 4530
Size: 1255 522 429 713 931 17 153 185
Cuts at: 4530 4975 325
Size: 445 1498
Fragments arranged by size:
1498 1255 931 713 522 445 429 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2669 4394 6106
Size: 274 80 33 51 1443 692 1725 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1725 1712 1443 692 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2961 2993 3181 3213 3252 3284 6108
Size: 32 854 32 188 32 39 32 2824
Cuts at: 6108 6140 2075
Size: 32 2083
Fragments arranged by size:
2824 2083 854 188 39 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 1316
Size: 1137 5011
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3442 3442
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 2581 1943
Size: 638 5510
XmaI C'CCGG_G
Cuts at: 1870 2029 2035 2972 3473 1870
Size: 159 6 937 501 4545
Fragments arranged by size:
4545 937 501 159 6
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacI SacII SexAI SfiI SwaI