Restriction map for
pFN21AB5823
(Circular) (Six-base) MAPSORT of: pFN21AB5823.seq Check: 7778 from: 1 to: 5149
pFN21AB5823 5149 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2959 278
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 1937
Size: 555 4594
Acc65I G'GTAC_C
Cuts at: 2470 2470
Size: 5149
AclI AA'CG_TT
Cuts at: 1563 3276 3649 1563
Size: 1713 373 3063
Fragments arranged by size:
3063 1713 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2379 3212 4596 4864 1632
Size: 578 169 833 1384 268 1917
Fragments arranged by size:
1917 1384 833 578 268 169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4952
AflIII A'CryG_T
Cuts at: 1203 4048 5025 1203
Size: 2845 977 1327
Fragments arranged by size:
2845 1327 977
AgeI A'CCGG_T
Cuts at: 3980 3980
Size: 5149
AhdI GACnn_n'nnGTC
Cuts at: 3878 3878
Size: 5149
AleI CACnn'nnGTG
Cuts at: 1166 2172 1166
Size: 1006 4143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 2892 4464 2892
Size: 1572 3577
ApaLI G'TGCA_C
Cuts at: 2165 3206 4362 2165
Size: 1041 1156 2952
Fragments arranged by size:
2952 1156 1041
ApoI r'AATT_y
Cuts at: 1493 2084 2458 2684 2836 4945 1493
Size: 591 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 591 374 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3703 160
Size: 872 2671 1606
Fragments arranged by size:
2671 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
AvaI C'yCGr_G
Cuts at: 1870 1943 2474 1870
Size: 73 531 4545
Fragments arranged by size:
4545 531 73
BamHI G'GATC_C
Cuts at: 2353 2479 2846 2353
Size: 126 367 4656
Fragments arranged by size:
4656 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 3925 618
Size: 325 217 627 683 1455 1842
Fragments arranged by size:
1842 1455 683 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2468 1391
Size: 1077 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3340 3374 1903
Size: 34 1403 34 3678
Fragments arranged by size:
3678 1403 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3041 4251 3041
Size: 1210 3939
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 4552
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3760 136
Size: 107 122 71 631 605 2088 1525
Fragments arranged by size:
2088 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5144 5144
Size: 5149
BlpI GC'TnA_GC
Cuts at: 2566 2566
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2067 2169 3210 4366 1790
Size: 259 18 102 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 259 102 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 3838 442
Size: 416 53 83 399 24 1463 958 1753
Fragments arranged by size:
1753 1463 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2019 1056
Size: 963 4186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 3809 1239
Size: 857 1713 2579
Fragments arranged by size:
2579 1713 857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3274 4139 4437 3274
Size: 865 298 3986
Fragments arranged by size:
3986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3812 882
Size: 617 2313 2219
Fragments arranged by size:
2313 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 2940 493
Size: 711 837 899 2702
Fragments arranged by size:
2702 899 837 711
BsaBI GATnn'nnATC
Cuts at: 2340 5143 2340
Size: 2803 2346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2956 3338 3973 275
Size: 53 83 186 936 1423 382 635 1451
Fragments arranged by size:
1451 1423 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3582 3980 4254 4401 4812 3582
Size: 398 274 147 411 3919
Fragments arranged by size:
3919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4924
Fragments arranged by size:
4924 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5149
BseYI C'CCAG_C
Cuts at: 4352 4848 4352
Size: 496 4653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4576
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2093 2599 3360 4388 1540
Size: 275 186 92 506 761 1028 2301
Fragments arranged by size:
2301 1028 761 506 275 186 92
BsiHKAI G_wGCw'C
Cuts at: 2169 2298 3210 3295 4366 2169
Size: 129 912 85 1071 2952
Fragments arranged by size:
2952 1071 912 129 85
BsmI GAATG_Cn'
Cuts at: 2660 2753 2660
Size: 93 5056
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2067 2169 2298 2468 3210 3295
Size: 399 259 18 102 129 170 742 85
Cuts at: 3295 4366 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 170 129 102
85 18
BspEI T'CCGG_A
Cuts at: 4812 4812
Size: 5149
BspHI T'CATG_A
Cuts at: 2142 3038 4768 2142
Size: 896 1730 2523
Fragments arranged by size:
2523 1730 896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2504 844
Size: 1193 467 3489
Fragments arranged by size:
3489 1193 467
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 1394
Size: 429 1213 3507
Fragments arranged by size:
3507 1213 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3644 3818 65
Size: 1183 2396 174 1396
Fragments arranged by size:
2396 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3793 3980 1700
Size: 110 1983 187 2869
Fragments arranged by size:
2869 1983 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5149
BssSI C'ACGA_G
Cuts at: 2066 3209 4221 2066
Size: 1143 1012 2994
Fragments arranged by size:
2994 1143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5149
BstBI TT'CG_AA
Cuts at: 2082 3953 2082
Size: 1871 3278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5149
BstYI r'GATC_y
Cuts at: 1972 2353 2479 2846 3231 3248 4700 4786 4798
Size: 381 126 367 385 17 1452 86 12
Cuts at: 4798 5144 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 381 367 346 126 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3658
Fragments arranged by size:
3658 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4410
Fragments arranged by size:
4410 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3459 3479 2665
Size: 794 20 4335
Fragments arranged by size:
4335 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5114
DraI TTT'AAA
Cuts at: 2453 2453
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4156 809
Size: 507 2840 1802
Fragments arranged by size:
2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2260 2596
Size: 54 1425 50 275 18 260 170 336
Cuts at: 2596 3485 5114 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 336 275 260 170 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2090 2596 1537
Size: 275 278 506 4090
Fragments arranged by size:
4090 506 278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 3079 1353
Size: 833 893 3423
Fragments arranged by size:
3423 893 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3736 4110 4256 387
Size: 1064 138 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2210 2379 3212 3809 4596 4864
Size: 393 464 114 169 833 597 787 268
Cuts at: 4864 1239
Size: 1524
Fragments arranged by size:
1524 833 787 597 464 393 268 169
114
EcoRI G'AATT_C
Cuts at: 2458 2836 2458
Size: 378 4771
FspI TGC'GCA
Cuts at: 3655 3655
Size: 5149
HaeII r_GCGC'y
Cuts at: 1678 4296 1678
Size: 2618 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 3790 3822 3864
Size: 32 135 32 713 32 1473 32 42
Cuts at: 3864 3896 1373
Size: 32 2626
Fragments arranged by size:
2626 1473 713 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2493 2739 669
Size: 988 281 555 246 3079
Fragments arranged by size:
3079 988 555 281 246
HindIII A'AGCT_T
Cuts at: 748 2102 748
Size: 1354 3795
HpaI GTT'AAC
Cuts at: 2739 2739
Size: 5149
KpnI G_GTAC'C
Cuts at: 2474 2474
Size: 5149
MfeI C'AATT_G
Cuts at: 2445 2748 2445
Size: 303 4846
MluI A'CGCG_T
Cuts at: 5025 5025
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4263 4447 1305
Size: 2958 184 2007
Fragments arranged by size:
2958 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3327
Fragments arranged by size:
3327 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2172 3107 3466 3625 518
Size: 648 843 163 935 359 159 2042
Fragments arranged by size:
2042 935 843 648 359 163 159
MspA1I CmG'CkG
Cuts at: 1606 2565 3242 4390 4635 1606
Size: 959 677 1148 245 2120
Fragments arranged by size:
2120 1148 959 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3658
Fragments arranged by size:
3658 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5149
NheI G'CTAG_C
Cuts at: 1052 2015 1052
Size: 963 4186
NotI GC'GGCC_GC
Cuts at: 2596 2596
Size: 5149
NspI r_CATG'y
Cuts at: 1132 1229 2507 4052 1132
Size: 97 1278 1545 2229
Fragments arranged by size:
2229 1545 1278 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5149
PciI A'CATG_T
Cuts at: 4048 4048
Size: 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2453 2453
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 4731 4763 3218
Size: 32 1481 32 3604
Fragments arranged by size:
3604 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5149
PsiI TTA'TAA
Cuts at: 2719 2719
Size: 5149
PstI C_TGCA'G
Cuts at: 830 2501 830
Size: 1671 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5149
SalI G'TCGA_C
Cuts at: 1936 2491 1936
Size: 555 4594
SbfI CC_TGCA'GG
Cuts at: 2501 2501
Size: 5149
ScaI AGT'ACT
Cuts at: 3397 5109 3397
Size: 1712 3437
SfcI C'TryA_G
Cuts at: 826 1045 2497 3632 4313 4504 826
Size: 219 1452 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5149
SmaI CCC'GGG
Cuts at: 1872 2476 1872
Size: 604 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3253 4154 4416 820
Size: 197 926 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2041 2940 493
Size: 1548 899 2702
Fragments arranged by size:
2702 1548 899
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5149
SphI G_CATG'C
Cuts at: 1132 2507 1132
Size: 1375 3774
SspI AAT'ATT
Cuts at: 5 52 3073 5
Size: 47 3021 2081
Fragments arranged by size:
3021 2081 47
StuI AGG'CCT
Cuts at: 1517 2364 1517
Size: 847 4302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2588 513
Size: 552 939 584 3074
Fragments arranged by size:
3074 939 584 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3176 3193 3346 3531 3976 325
Size: 1255 1596 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3395 5107 96
Size: 274 80 33 51 1443 1418 1712 138
Fragments arranged by size:
1712 1443 1418 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5109 5141 5109
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5149
XbaI T'CTAG_A
Cuts at: 2485 2485
Size: 5149
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5149
XmaI C'CCGG_G
Cuts at: 1870 2474 1870
Size: 604 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 3278 2364
Size: 914 4235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2957 276
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI