Restriction map for
pFN21AB5822
(Circular) (Six-base) MAPSORT of: pFN21AB5822.seq Check: 4832 from: 1 to: 6334
pFN21AB5822 6334 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3226 3226
Size: 6334
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3376 3592 4397 5781 6049 1632
Size: 1744 216 805 1384 268 1917
Fragments arranged by size:
1917 1744 1384 805 268 216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 2145 2590 5233 6210 1203
Size: 942 445 2643 977 1327
Fragments arranged by size:
2643 1327 977 942 445
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2240 1184
Size: 1056 5278
AlwNI CAG_nnn'CTG
Cuts at: 2128 2838 2879 3160 3253 4077 5649 2128
Size: 710 41 281 93 824 1572 2813
Fragments arranged by size:
2813 1572 824 710 281 93 41
ApaI G_GGCC'C
Cuts at: 2893 3272 2893
Size: 379 5955
ApaLI G'TGCA_C
Cuts at: 3179 4391 5547 3179
Size: 1212 1156 3966
Fragments arranged by size:
3966 1212 1156
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3174 3436 3572 3659 1870
Size: 73 1231 262 136 87 4545
Fragments arranged by size:
4545 1231 262 136 87 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3029 3457 3655 5110 618
Size: 325 217 627 1242 428 198 1455 1842
Fragments arranged by size:
1842 1455 1242 627 428 325 217 198
BanII G_rGCy'C
Cuts at: 1391 2067 2546 2871 2893 3272 3653 1391
Size: 676 479 325 22 379 381 4072
Fragments arranged by size:
4072 676 479 381 379 325 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 928
Size: 971 166 5197
Fragments arranged by size:
5197 971 166
BbvCI CC'TCA_GC
Cuts at: 3297 3297
Size: 6334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 4226 5436 3161
Size: 1065 1210 4059
Fragments arranged by size:
4059 1210 1065
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BfrBI ATG'CAT
Cuts at: 2299 2299
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 3584 4945
Size: 107 122 71 631 605 535 1377 1361
Cuts at: 4945 136
Size: 1525
Fragments arranged by size:
1525 1377 1361 631 605 535 122 107
71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3222 3751 3222
Size: 529 5805
Bme1580I G_kGCm'C
Cuts at: 1790 2893 3032 3183 3272 4395 5551 1790
Size: 1103 139 151 89 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 1103 151 139 89
BmgBI CAC'GTC
Cuts at: 1318 3013 1318
Size: 1695 4639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2093 4065 5023
Size: 416 53 83 399 24 676 1972 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1972 1753 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 3119 3140 3182 4994 1239
Size: 1214 666 21 42 1812 2579
Fragments arranged by size:
2579 1812 1214 666 42 21
Bpu10I CC'TnA_GC
Cuts at: 3297 3312 3404 3297
Size: 15 92 6227
Fragments arranged by size:
6227 92 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 4459 5324 5622 3023
Size: 1436 865 298 3735
Fragments arranged by size:
3735 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4997 882
Size: 617 993 2505 2219
Fragments arranged by size:
2505 2219 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 2148 2593 4125 493
Size: 711 944 445 1532 2702
Fragments arranged by size:
2702 1532 944 711 445
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2823 4141 4523
Size: 53 83 186 936 677 613 1318 382
Cuts at: 4523 5158 275
Size: 635 1451
Fragments arranged by size:
1451 1318 936 677 635 613 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3060 4767 5165 5439 5586 5997 3060
Size: 1707 398 274 147 411 3397
Fragments arranged by size:
3397 1707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 2477 2507 2639
Size: 30 165 30 729 30 315 30 132
Cuts at: 2639 2669 1178
Size: 30 4843
Fragments arranged by size:
4843 729 315 165 132 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2153 2276 2593 3632 1186
Size: 882 85 123 317 1039 3888
Fragments arranged by size:
3888 1039 882 317 123 85
BseYI C'CCAG_C
Cuts at: 2533 3231 3289 3360 3482 5537 6033 2533
Size: 698 58 71 122 2055 496 2834
Fragments arranged by size:
2834 2055 698 496 122 71 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 2630 1163
Size: 573 705 189 4867
Fragments arranged by size:
4867 705 573 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2623 2871 3183 4395 4480 5551 2623
Size: 248 312 1212 85 1071 3406
Fragments arranged by size:
3406 1212 1071 312 248 85
BsmI GAATG_Cn'
Cuts at: 2988 3845 3938 2988
Size: 857 93 5384
Fragments arranged by size:
5384 857 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2935 2935
Size: 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2546 2623 2871 2893 3032 3183
Size: 399 277 479 77 248 22 139 151
Cuts at: 3183 3272 3653 4395 4480 5551 1391
Size: 89 381 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 479 399 381 277 248
151 139 89 85 77 22
BspEI T'CCGG_A
Cuts at: 3060 5997 3060
Size: 2937 3397
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 3087 3226 3689 844
Size: 1325 918 139 463 3489
Fragments arranged by size:
3489 1325 918 463 139
BsrBI CCG'CTC
Cuts at: 1394 1823 2905 4221 1394
Size: 429 1082 1316 3507
Fragments arranged by size:
3507 1316 1082 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2194 4978 5165 1700
Size: 110 384 2784 187 2869
Fragments arranged by size:
2869 2784 384 187 110
BsrGI T'GTAC_A
Cuts at: 96 2784 96
Size: 2688 3646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 2305 2838 3253 794
Size: 1445 66 533 415 3875
Fragments arranged by size:
3875 1445 533 415 66
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 3007 3097 3007
Size: 90 6244
BstYI r'GATC_y
Cuts at: 1972 3499 3664 4031 4416 4433 5885 5971 5983
Size: 1527 165 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1527 1452 385 367 346 165 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2205 2634 513
Size: 552 1140 429 4213
Fragments arranged by size:
4213 1140 552 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3639 487
Size: 683 56 2413 3182
Fragments arranged by size:
3182 2413 683 56
BtsI GCAGTG_nn'
Cuts at: 3317 3527 3850 4644 4664 3317
Size: 210 323 794 20 4987
Fragments arranged by size:
4987 794 323 210 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 5341 809
Size: 507 1485 2540 1802
Fragments arranged by size:
2540 1802 1485 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2637 2895
Size: 54 1425 50 275 18 528 279 258
Cuts at: 2895 3781 4670 6299 8
Size: 886 889 1629 43
Fragments arranged by size:
1629 1425 889 886 528 279 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 3258 4264 1353
Size: 702 1203 1006 3423
Fragments arranged by size:
3423 1203 1006 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 3119 3140 3182 3376 3592 4397
Size: 393 821 666 21 42 194 216 805
Cuts at: 4397 4994 5781 6049 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 821 805 787 666 597 393 268
216 194 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2897 2897
Size: 6334
EcoO109I rG'GnC_Cy
Cuts at: 2889 2890 3076 3268 3280 3301 3431 2889
Size: 1 186 192 12 21 130 5792
Fragments arranged by size:
5792 192 186 130 21 12 1
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 2228 4840 2228
Size: 2612 3722
HaeII r_GCGC'y
Cuts at: 1678 2195 5481 1678
Size: 517 3286 2531
Fragments arranged by size:
3286 2531 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 3157 3189 4975
Size: 32 135 32 905 32 648 32 1786
Cuts at: 4975 5007 5049 5081 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1786 905 648 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 3924 669
Size: 988 281 1740 246 3079
Fragments arranged by size:
3079 1740 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 2682 5448 5632 1305
Size: 1350 27 2766 184 2007
Fragments arranged by size:
2766 2007 1350 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2639 2897 10
Size: 54 1425 343 807 258 3447
Fragments arranged by size:
3447 1425 807 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2778 3215 4292 4651 4810 518
Size: 648 1473 139 437 1077 359 159 2042
Fragments arranged by size:
2042 1473 1077 648 437 359 159 139
MspA1I CmG'CkG
Cuts at: 1606 2014 2269 2609 3346 3538 3750 4427 5575
Size: 408 255 340 737 192 212 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 737 677 408 340 255 245
212 192
NaeI GCC'GGC
Cuts at: 2196 2196
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 2205 2634 513
Size: 552 1140 429 4213
Fragments arranged by size:
4213 1140 552 429
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 2194 2194
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NsiI A_TGCA'T
Cuts at: 2301 2301
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2233 2281 2308 3692 5237 1132
Size: 97 1004 48 27 1384 1545 2229
Fragments arranged by size:
2229 1545 1384 1004 97 48 27
PasI CC'CwG_GG
Cuts at: 1348 2539 2573 3272 1348
Size: 1191 34 699 4410
Fragments arranged by size:
4410 1191 699 34
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3160 3238 3385 1108
Size: 2052 78 147 4057
Fragments arranged by size:
4057 2052 147 78
PfoI T'CCnGG_A
Cuts at: 3153 3516 3153
Size: 363 5971
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2148 2593 2148
Size: 445 5889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3076 3301 3431 3076
Size: 225 130 5979
Fragments arranged by size:
5979 225 130
PshAI GACnn'nnGTC
Cuts at: 1613 3429 1613
Size: 1816 4518
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspOMI G'GGCC_C
Cuts at: 2889 3268 2889
Size: 379 5955
PspXI vC'TCGA_Gb
Cuts at: 3174 3174
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2460 3686 830
Size: 1630 1226 3478
Fragments arranged by size:
3478 1630 1226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2014 2014
Size: 6334
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SanDI GG'GwC_CC
Cuts at: 3431 3431
Size: 6334
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 2456 3682 4817 5498 5689 826
Size: 219 1411 1226 1135 681 191 1471
Fragments arranged by size:
1471 1411 1226 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3584 3584
Size: 6334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3438 3661 1872
Size: 1566 223 4545
Fragments arranged by size:
4545 1566 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 3174 4438 5339 5601 820
Size: 197 926 1095 136 1264 901 262 1553
Fragments arranged by size:
1553 1264 1095 926 901 262 197 136
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 2233 2281 3692 1132
Size: 1101 48 1411 3774
Fragments arranged by size:
3774 1411 1101 48
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 3044 3578 1517
Size: 1527 534 4273
Fragments arranged by size:
4273 1527 534
StyI C'CwwG_G
Cuts at: 513 1065 2037 2205 2634 2716 2898 2994 3582
Size: 552 972 168 429 82 182 96 588
Cuts at: 3582 3773 513
Size: 191 3074
Fragments arranged by size:
3074 972 588 552 429 191 182 168
96 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 4361 4378 4531 4716 5161 325
Size: 1255 1794 987 17 153 185 445 1498
Fragments arranged by size:
1794 1498 1255 987 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4580 6292
Size: 274 80 33 51 1443 807 1796 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1796 1712 1443 807 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 2582 2614 2639 2671 6294 6326 2132
Size: 32 418 32 25 32 3623 32 2140
Fragments arranged by size:
3623 2140 418 32 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 1316 2711 1316
Size: 1395 4939
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 3499 2632
Size: 867 5467
XhoI C'TCGA_G
Cuts at: 1943 3174 1943
Size: 1231 5103
XmaI C'CCGG_G
Cuts at: 1870 3436 3659 1870
Size: 1566 223 4545
Fragments arranged by size:
4545 1566 223
XmnI GAAnn'nnTTC
Cuts at: 4463 4463
Size: 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI PacI PsrI RsrII SacII SapI SexAI SfoI
SrfI SwaI