Restriction map for
pFN21AB5820
(Circular) (Six-base) MAPSORT of: pFN21AB5820.seq Check: 728 from: 1 to: 6298
pFN21AB5820 6298 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2257 2257
Size: 6298
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3189 3641 1937
Size: 1252 452 4594
Fragments arranged by size:
4594 1252 452
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3076 3272 3374 4361 5745 6013 1632
Size: 1444 196 102 987 1384 268 1917
Fragments arranged by size:
1917 1444 1384 987 268 196 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 3455 1166
Size: 2289 4009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 3555 1184
Size: 1737 634 3927
Fragments arranged by size:
3927 1737 634
AlwNI CAG_nnn'CTG
Cuts at: 2645 3007 3568 3580 4041 5613 2645
Size: 362 561 12 461 1572 3330
Fragments arranged by size:
3330 1572 561 461 362 12
ApaI G_GGCC'C
Cuts at: 2165 2195 2204 3514 2165
Size: 30 9 1310 4949
Fragments arranged by size:
4949 1310 30 9
ApaLI G'TGCA_C
Cuts at: 2896 2972 4355 5511 2896
Size: 76 1383 1156 3683
Fragments arranged by size:
3683 1383 1156 76
ApoI r'AATT_y
Cuts at: 1493 3585 3607 3833 3985 6094 1493
Size: 2092 22 226 152 2109 1697
Fragments arranged by size:
2109 2092 1697 226 152 22
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 3623 1870
Size: 73 222 1458 4545
Fragments arranged by size:
4545 1458 222 73
BamHI G'GATC_C
Cuts at: 2341 3628 3995 2341
Size: 1287 367 4644
Fragments arranged by size:
4644 1287 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2826 3619 5074 618
Size: 325 217 627 273 766 793 1455 1842
Fragments arranged by size:
1842 1455 793 766 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2165 2195 2204 3514 3617 1391
Size: 774 30 9 1310 103 4072
Fragments arranged by size:
4072 1310 774 103 30 9
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3215 3362 928
Size: 971 1316 147 3864
Fragments arranged by size:
3864 1316 971 147
BbvCI CC'TCA_GC
Cuts at: 2442 2442
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 2190 2199
Size: 107 122 71 631 605 383 135 9
Cuts at: 2199 4909 136
Size: 2710 1525
Fragments arranged by size:
2710 1525 631 605 383 135 122 107
71 9
BglII A'GATC_T
Cuts at: 3087 6293 3087
Size: 3206 3092
BlpI GC'TnA_GC
Cuts at: 2045 3715 2045
Size: 1670 4628
Bme1580I G_kGCm'C
Cuts at: 1790 2165 2195 2204 2370 2900 2976 3048 3514
Size: 375 30 9 166 530 76 72 466
Cuts at: 3514 4359 5515 1790
Size: 845 1156 2573
Fragments arranged by size:
2573 1156 845 530 466 375 166 76
72 30 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 4029 4987
Size: 416 53 83 399 24 1509 1103 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
1753 1509 1103 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2898 4958 1239
Size: 1659 2060 2579
Fragments arranged by size:
2579 2060 1659
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 4423 5288 5586 3425
Size: 998 865 298 4137
Fragments arranged by size:
4137 998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2874 3323 3429 4961 882
Size: 617 1375 449 106 1532 2219
Fragments arranged by size:
2219 1532 1375 617 449 106
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 2540 6292 2540
Size: 3752 2546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 4105 4487 5122
Size: 53 83 186 936 528 2044 382 635
Cuts at: 5122 275
Size: 1451
Fragments arranged by size:
2044 1451 936 635 528 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2244 2829 4731 5129 5403 5550 5961 2244
Size: 585 1902 398 274 147 411 2581
Fragments arranged by size:
2581 1902 585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 1178
Size: 30 165 30 709 30 5334
Fragments arranged by size:
5334 709 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2428 3488 3491 1186
Size: 1242 1060 3 3993
Fragments arranged by size:
3993 1242 1060 3
BseYI C'CCAG_C
Cuts at: 2394 2615 2668 2965 3426 3580 5501 5997 2394
Size: 221 53 297 461 154 1921 496 2695
Fragments arranged by size:
2695 1921 496 461 297 221 154 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 2658 3569 1163
Size: 573 583 339 911 3892
Fragments arranged by size:
3892 911 583 573 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 2976 4359 4444 5515 2900
Size: 76 1383 85 1071 3683
Fragments arranged by size:
3683 1383 1071 85 76
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 3475 3475
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2195 2204 2370 2900 2976 3048
Size: 399 375 30 9 166 530 76 72
Cuts at: 3048 3514 3617 4359 4444 5515 1391
Size: 466 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 466 399 375 166
103 85 76 72 30 9
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 3653 844
Size: 1413 1396 3489
Fragments arranged by size:
3489 1413 1396
BsrBI CCG'CTC
Cuts at: 1394 1823 2503 3052 4185 1394
Size: 429 680 549 1133 3507
Fragments arranged by size:
3507 1133 680 549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2520 4793 4967 65
Size: 1183 1272 2273 174 1396
Fragments arranged by size:
2273 1396 1272 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 4942 5129 1700
Size: 110 312 2820 187 2869
Fragments arranged by size:
2869 2820 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 3047 4358 5370 3047
Size: 1311 1012 3975
Fragments arranged by size:
3975 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2645 2833 794
Size: 1851 188 4259
Fragments arranged by size:
4259 1851 188
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstXI CCAn_nnnn'nTGG
Cuts at: 2225 2338 3133 2225
Size: 113 795 5390
Fragments arranged by size:
5390 795 113
BstYI r'GATC_y
Cuts at: 1972 2341 2485 2541 2589 3087 3321 3628 3995
Size: 369 144 56 48 498 234 307 367
Cuts at: 3995 4380 4397 5849 5935 5947 6293 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 498 385 369 367 346 307
234 144 86 56 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3471 3471
Size: 6298
BtgI C'CryG_G
Cuts at: 513 1065 2064 2097 2187 2196 2205 2253 513
Size: 552 999 33 90 9 9 48 4558
Fragments arranged by size:
4558 999 552 90 48 33 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 3814 4608 4628 3814
Size: 794 20 5484
Fragments arranged by size:
5484 794 20
ClaI AT'CG_AT
Cuts at: 1554 3228 1554
Size: 1674 4624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DraIII CAC_nnn'GTG
Cuts at: 2818 2818
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2888 3164 3337 5305 809
Size: 507 1572 276 173 1968 1802
Fragments arranged by size:
1968 1802 1572 507 276 173
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3745 4634
Size: 54 1425 50 275 18 1270 645 889
Cuts at: 4634 6263 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1270 889 645 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 4228 1353
Size: 1382 1493 3423
Fragments arranged by size:
3423 1493 1382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 2639 3071 4885 5259 5405
Size: 1064 138 582 468 432 1814 374 146
Cuts at: 5405 387
Size: 1280
Fragments arranged by size:
1814 1280 1064 582 468 432 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2898 3076 3272 3374 4361 4958 5745
Size: 393 1266 178 196 102 987 597 787
Cuts at: 5745 6013 1239
Size: 268 1524
Fragments arranged by size:
1524 1266 987 787 597 393 268 196
178 102
EcoO109I rG'GnC_Cy
Cuts at: 2102 2161 2162 2192 2201 2273 2652 3004 2102
Size: 59 1 30 9 72 379 352 5396
Fragments arranged by size:
5396 379 352 72 59 30 9 1
EcoRI G'AATT_C
Cuts at: 3585 3607 3985 3585
Size: 22 378 5898
Fragments arranged by size:
5898 378 22
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 2064 2087 2129 2401 3433 5445 1678
Size: 386 23 42 272 1032 2012 2531
Fragments arranged by size:
2531 2012 1032 386 272 42 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 2509 2541 3451
Size: 32 135 32 540 32 365 32 910
Cuts at: 3451 3483 4939 4971 5013 5045 1373
Size: 32 1456 32 42 32 2626
Fragments arranged by size:
2626 1456 910 540 365 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3190 3642 3888 669
Size: 988 281 1252 452 246 3079
Fragments arranged by size:
3079 1252 988 452 281 246
HindIII A'AGCT_T
Cuts at: 748 2582 748
Size: 1834 4464
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5412 5596 1305
Size: 4107 184 2007
Fragments arranged by size:
4107 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4476
Fragments arranged by size:
4476 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 3230 3455 4256 4615 4774 518
Size: 648 1170 894 225 801 359 159 2042
Fragments arranged by size:
2042 1170 894 801 648 359 225 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2099 2198 2207 3034 3352 3714 4391
Size: 429 64 99 9 827 318 362 677
Cuts at: 4391 5539 5784 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 827 677 429 362 318 245
99 64 9
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2187 2253 513
Size: 552 1122 66 4558
Fragments arranged by size:
4558 1122 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 2113 2233 2326 3656 5201 1132
Size: 97 884 120 93 1330 1545 2229
Fragments arranged by size:
2229 1545 1330 884 120 97 93
PasI CC'CwG_GG
Cuts at: 1348 2077 2657 3507 1348
Size: 729 580 850 4139
Fragments arranged by size:
4139 850 729 580
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6298
PfoI T'CCnGG_A
Cuts at: 2451 2544 3408 2451
Size: 93 864 5341
Fragments arranged by size:
5341 864 93
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2273 2652 2102
Size: 171 379 5748
Fragments arranged by size:
5748 379 171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PspOMI G'GGCC_C
Cuts at: 2161 2191 2200 3510 2161
Size: 30 9 1310 4949
Fragments arranged by size:
4949 1310 30 9
PstI C_TGCA'G
Cuts at: 830 2650 3039 3150 3650 830
Size: 1820 389 111 500 3478
Fragments arranged by size:
3478 1820 500 389 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 3034 3352 3034
Size: 318 5980
RsrII CG'GwC_CG
Cuts at: 2120 2120
Size: 6298
SacII CC_GC'GG
Cuts at: 2100 2199 2208 2100
Size: 99 9 6190
Fragments arranged by size:
6190 99 9
SalI G'TCGA_C
Cuts at: 1936 3188 3640 1936
Size: 1252 452 4594
Fragments arranged by size:
4594 1252 452
SanDI GG'GwC_CC
Cuts at: 2102 2273 2652 2102
Size: 171 379 5748
Fragments arranged by size:
5748 379 171
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SexAI A'CCwGG_T
Cuts at: 2814 2814
Size: 6298
SfcI C'TryA_G
Cuts at: 826 1045 2646 3035 3146 3646 4781 5462 5653
Size: 219 1601 389 111 500 1135 681 191
Cuts at: 5653 826
Size: 1471
Fragments arranged by size:
1601 1471 1135 681 500 389 219 191
111
SfiI GGCCn_nnn'nGGCC
Cuts at: 2199 2199
Size: 6298
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 2167 3625 1872
Size: 295 1458 4545
Fragments arranged by size:
4545 1458 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3440 4402 5303 5565 820
Size: 197 926 1497 962 901 262 1553
Fragments arranged by size:
1553 1497 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 2113 2326 3656 1132
Size: 981 213 1330 3774
Fragments arranged by size:
3774 1330 981 213
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2187 2253 3543 3737 513
Size: 552 1122 66 1290 194 3074
Fragments arranged by size:
3074 1290 1122 552 194 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2858 4325 4342 4495 4680 5125
Size: 1255 764 514 1467 17 153 185 445
Cuts at: 5125 325
Size: 1498
Fragments arranged by size:
1498 1467 1255 764 514 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2934 3057 4544
Size: 274 80 33 51 1443 957 123 1487
Cuts at: 4544 6256 96
Size: 1712 138
Fragments arranged by size:
1712 1487 1443 957 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6258 6290 6258
Size: 32 6266
Tth111I GACn'n_nGTC
Cuts at: 1316 2139 1316
Size: 823 5475
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2852 3038 2852
Size: 186 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 2165 3623 1870
Size: 295 1458 4545
Fragments arranged by size:
4545 1458 295
XmnI GAAnn'nnTTC
Cuts at: 4427 4427
Size: 6298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI SacI SapI
SrfI SwaI