Restriction map for
pFN21AB5816
(Circular) (Six-base) MAPSORT of: pFN21AB5816.seq Check: 6191 from: 1 to: 7330
pFN21AB5816 7330 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2104 2364 5140 278
Size: 53 83 186 936 568 260 2776 2468
Fragments arranged by size:
2776 2468 936 568 260 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4673 1937
Size: 2736 4594
Acc65I G'GTAC_C
Cuts at: 3330 4651 3330
Size: 1321 6009
AclI AA'CG_TT
Cuts at: 1563 5457 5830 1563
Size: 3894 373 3063
Fragments arranged by size:
3894 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5393 6777 7045 1632
Size: 3761 1384 268 1917
Fragments arranged by size:
3761 1917 1384 268
AfeI AGC'GCT
Cuts at: 2198 2516 3168 2198
Size: 318 652 6360
Fragments arranged by size:
6360 652 318
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7133
AflIII A'CryG_T
Cuts at: 1203 6229 7206 1203
Size: 5026 977 1327
Fragments arranged by size:
5026 1327 977
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 2669 6059 2669
Size: 3390 3940
AleI CACnn'nnGTG
Cuts at: 1166 3075 1166
Size: 1909 5421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7330
AlwNI CAG_nnn'CTG
Cuts at: 3113 4123 4384 5073 6645 3113
Size: 1010 261 689 1572 3798
Fragments arranged by size:
3798 1572 1010 689 261
ApaI G_GGCC'C
Cuts at: 2324 2653 3945 2324
Size: 329 1292 5709
Fragments arranged by size:
5709 1292 329
ApaLI G'TGCA_C
Cuts at: 5387 6543 5387
Size: 1156 6174
ApoI r'AATT_y
Cuts at: 1493 4163 4639 4865 5017 7126 1493
Size: 2670 476 226 152 2109 1697
Fragments arranged by size:
2670 2109 1697 476 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5884 160
Size: 872 4852 1606
Fragments arranged by size:
4852 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3300 4655 1870
Size: 73 700 657 1355 4545
Fragments arranged by size:
4545 1355 700 657 73
AvrII C'CTAG_G
Cuts at: 2887 2887
Size: 7330
BamHI G'GATC_C
Cuts at: 4660 5027 4660
Size: 367 6963
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3330 4651 6106 618
Size: 325 217 627 1543 1321 1455 1842
Fragments arranged by size:
1842 1543 1455 1321 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2324 2653 2853 3366 3482 3945 4649 1391
Size: 933 329 200 513 116 463 704 4072
Fragments arranged by size:
4072 933 704 513 463 329 200 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 2665 2940 3027 3998 928
Size: 971 560 206 275 87 971 4260
Fragments arranged by size:
4260 971 971 560 275 206 87
BbvCI CC'TCA_GC
Cuts at: 2340 3141 2340
Size: 801 6529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5521 5555 1903
Size: 34 3584 34 3678
Fragments arranged by size:
3678 3584 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5222 6432 5222
Size: 1210 6120
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 5941 136
Size: 107 122 71 631 605 976 3293 1525
Fragments arranged by size:
3293 1525 976 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3432 7325 3432
Size: 3893 3437
BlpI GC'TnA_GC
Cuts at: 3476 4272 4747 3476
Size: 796 475 6059
Fragments arranged by size:
6059 796 475
Bme1580I G_kGCm'C
Cuts at: 1790 2324 2653 3945 5391 6547 1790
Size: 534 329 1292 1446 1156 2573
Fragments arranged by size:
2573 1446 1292 1156 534 329
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2263 4133 5061
Size: 416 53 83 399 24 846 1870 928
Cuts at: 5061 6019 442
Size: 958 1753
Fragments arranged by size:
1870 1753 958 928 846 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2284 3331 4057 2218
Size: 66 1047 726 5491
Fragments arranged by size:
5491 1047 726 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2828 2898 3251 4001 5990 1239
Size: 1589 70 353 750 1989 2579
Fragments arranged by size:
2579 1989 1589 750 353 70
Bpu10I CC'TnA_GC
Cuts at: 2340 3141 2340
Size: 801 6529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3155 5455 6320 6618 3155
Size: 2300 865 298 3867
Fragments arranged by size:
3867 2300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4563 5993 882
Size: 617 3064 1430 2219
Fragments arranged by size:
3064 2219 1430 617
BsaAI yAC'GTr
Cuts at: 493 1204 5121 493
Size: 711 3917 2702
Fragments arranged by size:
3917 2702 711
BsaBI GATnn'nnATC
Cuts at: 7324 7324
Size: 7330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2361 3219 3632
Size: 53 83 186 936 568 260 858 413
Cuts at: 3632 5137 5519 6154 275
Size: 1505 382 635 1451
Fragments arranged by size:
1505 1451 936 858 635 568 413 382
260 186 83 53
BsaWI w'CCGG_w
Cuts at: 2181 2541 3063 5763 6161 6435 6582 6993 2181
Size: 360 522 2700 398 274 147 411 2518
Fragments arranged by size:
2700 2518 522 411 398 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7105
Fragments arranged by size:
7105 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2462 2600 2831 2834 2837 2840 3158
Size: 1074 202 138 231 3 3 3 318
Cuts at: 3158 3161 3170 3391 3731 3734 3737 3741 3773
Size: 3 9 221 340 3 3 4 32
Cuts at: 3773 3827 4016 1186
Size: 54 189 4500
Fragments arranged by size:
4500 1074 340 318 231 221 202 189
138 54 32 9 4 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2306 2404 2852 3366 3396 3534 3730 4279 4305
Size: 98 448 514 30 138 196 549 26
Cuts at: 4305 6533 7029 2306
Size: 2228 496 2607
Fragments arranged by size:
2607 2228 549 514 496 448 196 138
98 30 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6757
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 2077 3248 3412 4780 5541
Size: 275 186 31 45 1171 164 1368 761
Cuts at: 5541 6569 1540
Size: 1028 2301
Fragments arranged by size:
2301 1368 1171 1028 761 275 186 164
45 31
BsiHKAI G_wGCw'C
Cuts at: 2265 5391 5476 6547 2265
Size: 3126 85 1071 3048
Fragments arranged by size:
3126 3048 1071 85
BsmI GAATG_Cn'
Cuts at: 3859 4841 4934 3859
Size: 982 93 6255
Fragments arranged by size:
6255 982 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2492 3464 2492
Size: 972 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2324 2653 2853 3366 3482 3945
Size: 399 475 59 329 200 513 116 463
Cuts at: 3945 4649 5391 5476 6547 1391
Size: 704 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 513 475 463 399
329 200 116 85 59
BspEI T'CCGG_A
Cuts at: 2541 6993 2541
Size: 4452 2878
BspHI T'CATG_A
Cuts at: 5219 6949 5219
Size: 1730 5600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 3453 3855 4685 844
Size: 2425 184 402 830 3489
Fragments arranged by size:
3489 2425 830 402 184
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 3802 5217 1394
Size: 429 1309 670 1415 3507
Fragments arranged by size:
3507 1415 1309 670 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 5825 5999 65
Size: 1183 1164 3413 174 1396
Fragments arranged by size:
3413 1396 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5974 6161 1700
Size: 110 4164 187 2869
Fragments arranged by size:
4164 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7330
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 3646 5390 6402 3646
Size: 1744 1012 4574
Fragments arranged by size:
4574 1744 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2554 3550 794
Size: 1760 996 4574
Fragments arranged by size:
4574 1760 996
BstBI TT'CG_AA
Cuts at: 6134 6134
Size: 7330
BstEII G'GTnAC_C
Cuts at: 1184 3400 1184
Size: 2216 5114
BstXI CCAn_nnnn'nTGG
Cuts at: 4313 4313
Size: 7330
BstYI r'GATC_y
Cuts at: 1972 3432 4660 5027 5412 5429 6881 6967 6979
Size: 1460 1228 367 385 17 1452 86 12
Cuts at: 6979 7325 1972
Size: 346 1977
Fragments arranged by size:
1977 1460 1452 1228 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3756 3768 3756
Size: 12 7318
BtgI C'CryG_G
Cuts at: 513 1065 2004 2137 3222 3242 513
Size: 552 939 133 1085 20 4601
Fragments arranged by size:
4601 1085 939 552 133 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3268 487
Size: 683 56 2042 4549
Fragments arranged by size:
4549 2042 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4846 5640 5660 3421
Size: 1425 794 20 5091
Fragments arranged by size:
5091 1425 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3901 3936 529
Size: 35 3337 35 3923
Fragments arranged by size:
3923 3337 35 35
DraI TTT'AAA
Cuts at: 4634 4634
Size: 7330
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6337 809
Size: 507 5021 1802
Fragments arranged by size:
5021 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 3133 3245
Size: 54 1425 50 275 18 310 993 112
Cuts at: 3245 3573 4777 5666 7295 8
Size: 328 1204 889 1629 43
Fragments arranged by size:
1629 1425 1204 993 889 328 310 275
112 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3245 4777 1537
Size: 275 1433 1532 4090
Fragments arranged by size:
4090 1532 1433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2050 3003 3275 4044 5260 1353
Size: 697 953 272 769 1216 3423
Fragments arranged by size:
3423 1216 953 769 697 272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4430 5917 6291 6437 387
Size: 1064 138 2841 1487 374 146 1280
Fragments arranged by size:
2841 1487 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2828 2898 3251 4001 5393 5990 6777
Size: 393 1196 70 353 750 1392 597 787
Cuts at: 6777 7045 1239
Size: 268 1524
Fragments arranged by size:
1524 1392 1196 787 750 597 393 353
268 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2810 3698 3771 2810
Size: 888 73 6369
Fragments arranged by size:
6369 888 73
EcoO109I rG'GnC_Cy
Cuts at: 2320 2649 2650 3496 3941 4172 4381 4463 4597
Size: 329 1 846 445 231 209 82 134
Cuts at: 4597 2320
Size: 5053
Fragments arranged by size:
5053 846 445 329 231 209 134 82
1
EcoRI G'AATT_C
Cuts at: 4639 5017 4639
Size: 378 6952
FspI TGC'GCA
Cuts at: 3278 3554 3851 4187 5836 3278
Size: 276 297 336 1649 4772
Fragments arranged by size:
4772 1649 336 297 276
HaeII r_GCGC'y
Cuts at: 1678 2152 2200 2518 3170 3356 3714 3750 4279
Size: 474 48 318 652 186 358 36 529
Cuts at: 4279 6477 1678
Size: 2198 2531
Fragments arranged by size:
2531 2198 652 529 474 358 318 186
48 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2655 2687 3101 3107 3133
Size: 32 135 32 1083 32 414 6 26
Cuts at: 3133 3139 3898 3930 5971 6003 6045 6077 1373
Size: 6 759 32 2041 32 42 32 2626
Fragments arranged by size:
2626 2041 1083 759 414 135 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3421 4674 4920 669
Size: 988 281 1483 1253 246 3079
Fragments arranged by size:
3079 1483 1253 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7330
HpaI GTT'AAC
Cuts at: 3421 4920 3421
Size: 1499 5831
KpnI G_GTAC'C
Cuts at: 3334 4655 3334
Size: 1321 6009
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 7206 7206
Size: 7330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 3099 3419 4134 6444 6628 1305
Size: 1662 132 320 715 2310 184 2007
Fragments arranged by size:
2310 2007 1662 715 320 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5508
Fragments arranged by size:
5508 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2613 3075 5288 5647 5806 518
Size: 648 1414 33 462 2213 359 159 2042
Fragments arranged by size:
2213 2042 1414 648 462 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 3224 3244 3250 3741 4746 5423 6571 6816
Size: 1618 20 6 491 1005 677 1148 245
Cuts at: 6816 1606
Size: 2120
Fragments arranged by size:
2120 1618 1148 1005 677 491 245 20
6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5839
Fragments arranged by size:
5839 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7330
NotI GC'GGCC_GC
Cuts at: 3245 4777 3245
Size: 1532 5798
NspI r_CATG'y
Cuts at: 1132 1229 4211 4688 6233 1132
Size: 97 2982 477 1545 2229
Fragments arranged by size:
2982 2229 1545 477 97
PasI CC'CwG_GG
Cuts at: 1348 2346 4378 1348
Size: 998 2032 4300
Fragments arranged by size:
4300 2032 998
PciI A'CATG_T
Cuts at: 6229 6229
Size: 7330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 3592 4063 1108
Size: 1222 1262 471 4375
Fragments arranged by size:
4375 1262 1222 471
PfoI T'CCnGG_A
Cuts at: 2715 2805 3462 2715
Size: 90 657 6583
Fragments arranged by size:
6583 657 90
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5399 5431 6912 6944 5399
Size: 32 1481 32 5785
Fragments arranged by size:
5785 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3496 4172 4597 3496
Size: 676 425 6229
Fragments arranged by size:
6229 676 425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7330
PsiI TTA'TAA
Cuts at: 4900 4900
Size: 7330
PspOMI G'GGCC_C
Cuts at: 2320 2649 3941 2320
Size: 329 1292 5709
Fragments arranged by size:
5709 1292 329
PstI C_TGCA'G
Cuts at: 830 3450 4450 4682 830
Size: 2620 1000 232 3478
Fragments arranged by size:
3478 2620 1000 232
PvuI CG_AT'CG
Cuts at: 2001 2032 2001
Size: 31 7299
SacII CC_GC'GG
Cuts at: 3225 3245 3225
Size: 20 7310
SalI G'TCGA_C
Cuts at: 1936 4672 1936
Size: 2736 4594
SbfI CC_TGCA'GG
Cuts at: 4682 4682
Size: 7330
ScaI AGT'ACT
Cuts at: 5578 7290 5578
Size: 1712 5618
SfcI C'TryA_G
Cuts at: 826 1045 3446 4446 4678 5813 6494 6685 826
Size: 219 2401 1000 232 1135 681 191 1471
Fragments arranged by size:
2401 1471 1135 1000 681 232 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2648 2648
Size: 7330
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 2645 3302 4657 1872
Size: 773 657 1355 4545
Fragments arranged by size:
4545 1355 773 657
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3170 5434 6335 6597 820
Size: 197 926 1227 2264 901 262 1553
Fragments arranged by size:
2264 1553 1227 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4211 4688 1132
Size: 3079 477 3774
Fragments arranged by size:
3774 3079 477
SspI AAT'ATT
Cuts at: 5 52 5254 5
Size: 47 5202 2081
Fragments arranged by size:
5202 2081 47
StuI AGG'CCT
Cuts at: 1517 2886 1517
Size: 1369 5961
StyI C'CwwG_G
Cuts at: 513 1065 2004 2887 3189 3213 3864 4574 4769
Size: 552 939 883 302 24 651 710 195
Cuts at: 4769 513
Size: 3074
Fragments arranged by size:
3074 939 883 710 651 552 302 195
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2420 2586 2625 3388 3548 3967
Size: 1255 417 423 166 39 763 160 419
Cuts at: 3967 5357 5374 5527 5712 6157 325
Size: 1390 17 153 185 445 1498
Fragments arranged by size:
1498 1390 1255 763 445 423 419 417
185 166 160 153 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5576 7288 96
Size: 274 80 33 51 1443 3599 1712 138
Fragments arranged by size:
3599 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3058 3090 4549 4581 7290 7322 3058
Size: 32 1459 32 2709 32 3066
Fragments arranged by size:
3066 2709 1459 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7330
XbaI T'CTAG_A
Cuts at: 4666 4666
Size: 7330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4484 4484
Size: 7330
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7330
XmaI C'CCGG_G
Cuts at: 1870 2643 3300 4655 1870
Size: 773 657 1355 4545
Fragments arranged by size:
4545 1355 773 657
XmnI GAAnn'nnTTC
Cuts at: 2456 5459 2456
Size: 3003 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2102 2362 5138 276
Size: 53 83 186 936 568 260 2776 2468
Fragments arranged by size:
2776 2468 936 568 260 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI
SwaI