Restriction map for
pFN21AB5813
(Circular) (Six-base) MAPSORT of: pFN21AB5813.seq Check: 142 from: 1 to: 6127
pFN21AB5813 6127 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2272 2555 2615 3117 3277 2272
Size: 283 60 502 160 5122
Fragments arranged by size:
5122 502 283 160 60
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2643 3937 278
Size: 53 83 186 936 1107 1294 2468
Fragments arranged by size:
2468 1294 1107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 3470 1937
Size: 166 1367 4594
Fragments arranged by size:
4594 1367 166
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 4190 5574 5842 1632
Size: 739 1819 1384 268 1917
Fragments arranged by size:
1917 1819 1384 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2445 2619 3000 5026 6003 1203
Size: 1242 174 381 2026 977 1327
Fragments arranged by size:
2026 1327 1242 977 381 174
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2873 1184
Size: 1689 4438
AlwNI CAG_nnn'CTG
Cuts at: 2695 3123 3870 5442 2695
Size: 428 747 1572 3380
Fragments arranged by size:
3380 1572 747 428
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2304 2423 3452 1870
Size: 73 270 91 119 1029 4545
Fragments arranged by size:
4545 1029 270 119 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2432 2465 2432
Size: 33 6094
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 3105 3289 3448 4903
Size: 325 217 627 756 562 184 159 1455
Cuts at: 4903 618
Size: 1842
Fragments arranged by size:
1842 1455 756 627 562 325 217 184
159
BanII G_rGCy'C
Cuts at: 1391 2334 2348 2503 2843 2970 3054 3446 1391
Size: 943 14 155 340 127 84 392 4072
Fragments arranged by size:
4072 943 392 340 155 127 84 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2635 928
Size: 971 736 4420
Fragments arranged by size:
4420 971 736
BbvCI CC'TCA_GC
Cuts at: 3234 3234
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2150 2184 4318 4352 1903
Size: 34 213 34 2134 34 3678
Fragments arranged by size:
3678 2134 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 3103 3319
Size: 107 122 71 631 605 703 728 216
Cuts at: 3319 4738 136
Size: 1419 1525
Fragments arranged by size:
1525 1419 728 703 631 605 216 122
107 71
BglII A'GATC_T
Cuts at: 3060 3325 6122 3060
Size: 265 2797 3065
Fragments arranged by size:
3065 2797 265
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2546 2619 3108 3294 4188 5344 1790
Size: 756 73 489 186 894 1156 2573
Fragments arranged by size:
2573 1156 894 756 489 186 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3037 3858 4816
Size: 416 53 83 399 24 1620 821 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1753 1620 958 821 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2332 2503 2921 3375 4787 1239
Size: 1046 47 171 418 454 1412 2579
Fragments arranged by size:
2579 1412 1046 454 418 171 47
Bpu10I CC'TnA_GC
Cuts at: 3234 3234
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 4252 5117 5415 2663
Size: 1589 865 298 3375
Fragments arranged by size:
3375 1589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2620 3001 3918 493
Size: 711 1416 381 917 2702
Fragments arranged by size:
2702 1416 917 711 381
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2640 3934 4316 4951
Size: 53 83 186 936 1107 1294 382 635
Cuts at: 4951 275
Size: 1451
Fragments arranged by size:
1451 1294 1107 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2476 2506 1178
Size: 30 165 30 1073 30 4799
Fragments arranged by size:
4799 1073 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 3023 3294 1186
Size: 1480 357 271 4019
Fragments arranged by size:
4019 1480 357 271
BseYI C'CCAG_C
Cuts at: 2798 2985 3189 3280 3354 3400 5330 5826 2798
Size: 187 204 91 74 46 1930 496 3099
Fragments arranged by size:
3099 1930 496 204 187 91 74 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2554 1163
Size: 573 657 161 4736
Fragments arranged by size:
4736 657 573 161
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 3426 3577 4338 5366 1540
Size: 275 186 198 1227 151 761 1028 2301
Fragments arranged by size:
2301 1227 1028 761 275 198 186 151
BsiHKAI G_wGCw'C
Cuts at: 2334 2843 2898 2970 3054 4188 4273 5344 2334
Size: 509 55 72 84 1134 85 1071 3117
Fragments arranged by size:
3117 1134 1071 509 85 84 72 55
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2348 2503 2546 2619 2843 2898
Size: 399 544 14 155 43 73 224 55
Cuts at: 2898 2970 3054 3108 3294 3446 4188 4273 5344
Size: 72 84 54 186 152 742 85 1071
Cuts at: 5344 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 544 399 224 186 155
152 85 84 73 72 55 54 43
14
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2272 2555 2615 3117 3167 3277 3482
Size: 1307 121 283 60 502 50 110 205
Cuts at: 3482 844
Size: 3489
Fragments arranged by size:
3489 1307 502 283 205 121 110 60
50
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2102 3308 4622 4796 65
Size: 1183 854 1206 1314 174 1396
Fragments arranged by size:
1396 1314 1206 1183 854 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 4771 4958 1700
Size: 110 388 2573 187 2869
Fragments arranged by size:
2869 2573 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3123 794
Size: 2329 3798
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 3060 3325 3457 3824 4209 4226 5678 5764
Size: 1088 265 132 367 385 17 1452 86
Cuts at: 5764 5776 6122 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1088 385 367 346 265 132
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 2663 3033 3217 3317 513
Size: 552 939 20 639 370 184 100 3323
Fragments arranged by size:
3323 939 639 552 370 184 100 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 3259 3643 4437 4457 2825
Size: 434 384 794 20 4495
Fragments arranged by size:
4495 794 434 384 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DraIII CAC_nnn'GTG
Cuts at: 2557 2557
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2256 2286
Size: 54 1425 50 275 18 366 60 30
Cuts at: 2286 2367 2376 2666 2971 3320 3423 3574 4463
Size: 81 9 290 305 349 103 151 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 366 349 305 290 275
151 103 81 60 54 50 43 30
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2196 3423 3574 1537
Size: 275 384 1227 151 4090
Fragments arranged by size:
4090 1227 384 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2085 2339 2423 4057 1353
Size: 684 48 254 84 1634 3423
Fragments arranged by size:
3423 1634 684 254 84 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2217 4714 5088 5234 387
Size: 1064 138 628 2497 374 146 1280
Fragments arranged by size:
2497 1280 1064 628 374 146 138
EcoICRI GAG'CTC
Cuts at: 2332 2841 2968 3052 2332
Size: 509 127 84 5407
Fragments arranged by size:
5407 509 127 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2332 2371 2503 2921 3375 4190
Size: 393 653 47 39 132 418 454 815
Cuts at: 4190 4787 5574 5842 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 815 787 653 597 454 418 393
268 132 47 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2482 2888 2482
Size: 406 5721
EcoO109I rG'GnC_Cy
Cuts at: 2140 2219 2464 3404 3412 2140
Size: 79 245 940 8 4855
Fragments arranged by size:
4855 940 245 79 8
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 6127
FspI TGC'GCA
Cuts at: 2588 4633 2588
Size: 2045 4082
HaeII r_GCGC'y
Cuts at: 1678 2086 5274 1678
Size: 408 3188 2531
Fragments arranged by size:
3188 2531 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 4768 4800 4842
Size: 32 135 32 944 32 2220 32 42
Cuts at: 4842 4874 1373
Size: 32 2626
Fragments arranged by size:
2626 2220 944 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 2704 3471 3717 669
Size: 988 281 166 600 767 246 3079
Fragments arranged by size:
3079 988 767 600 281 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3255 5241 5425 1305
Size: 1950 1986 184 2007
Fragments arranged by size:
2007 1986 1950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 2369 2378 2668 10
Size: 54 1425 343 426 111 9 290 3469
Fragments arranged by size:
3469 1425 426 343 290 111 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 4085 4444 4603 518
Size: 648 1833 1086 359 159 2042
Fragments arranged by size:
2042 1833 1086 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2365 2613 2802 3016 3120 3174 3395
Size: 558 201 248 189 214 104 54 221
Cuts at: 3395 3543 4220 5368 5613 1606
Size: 148 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 248 245 221 214
201 189 148 104 54
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 2004 2663 3033 513
Size: 552 939 659 370 3607
Fragments arranged by size:
3607 939 659 552 370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2398 2449 3485 5030 1132
Size: 97 1169 51 1036 1545 2229
Fragments arranged by size:
2229 1545 1169 1036 97 51
PasI CC'CwG_GG
Cuts at: 1348 2056 2517 1348
Size: 708 461 4958
Fragments arranged by size:
4958 708 461
PciI A'CATG_T
Cuts at: 2445 5026 2445
Size: 2581 3546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 1108
Size: 1449 4678
PfoI T'CCnGG_A
Cuts at: 2898 2898
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PmlI CAC'GTG
Cuts at: 2620 3001 2620
Size: 381 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2219 3404 3412 2140
Size: 79 1185 8 4855
Fragments arranged by size:
4855 1185 79 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PstI C_TGCA'G
Cuts at: 830 2082 2612 2760 2768 2862 3021 3119 3479
Size: 1252 530 148 8 94 159 98 360
Cuts at: 3479 830
Size: 3478
Fragments arranged by size:
3478 1252 530 360 159 148 98 94
8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2164 2365 2802 3016 3120 3395 2164
Size: 201 437 214 104 275 4896
Fragments arranged by size:
4896 437 275 214 201 104
SacI G_AGCT'C
Cuts at: 2334 2843 2970 3054 2334
Size: 509 127 84 5407
Fragments arranged by size:
5407 509 127 84
SalI G'TCGA_C
Cuts at: 1936 2102 3469 1936
Size: 166 1367 4594
Fragments arranged by size:
4594 1367 166
SanDI GG'GwC_CC
Cuts at: 2219 3404 2219
Size: 1185 4942
SapI GCTCTTCn'nnn_
Cuts at: 2085 2339 2085
Size: 254 5873
SbfI CC_TGCA'GG
Cuts at: 2768 3479 2768
Size: 711 5416
ScaI AGT'ACT
Cuts at: 2435 4375 6087 2435
Size: 1940 1712 2475
Fragments arranged by size:
2475 1940 1712
SfcI C'TryA_G
Cuts at: 826 1045 2078 2608 2756 2764 2858 3017 3115
Size: 219 1033 530 148 8 94 159 98
Cuts at: 3115 3475 4610 5291 5482 826
Size: 360 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 530 360 219 191
159 148 98 94 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 2375 2375
Size: 6127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 2215 2306 2425 3454 1872
Size: 343 91 119 1029 4545
Fragments arranged by size:
4545 1029 343 119 91
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 4231 5132 5394 820
Size: 197 926 735 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4882
StyI C'CwwG_G
Cuts at: 513 1065 2004 2460 2663 3033 3566 513
Size: 552 939 456 203 370 533 3074
Fragments arranged by size:
3074 939 552 533 456 370 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2724 4154 4171 4324 4509 4954 325
Size: 1255 1144 1430 17 153 185 445 1498
Fragments arranged by size:
1498 1430 1255 1144 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 2804 4373
Size: 274 80 33 51 1443 456 371 1569
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1712 1569 1443 456 371 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3238 3270 6087 6119 3238
Size: 32 2817 32 3246
Fragments arranged by size:
3246 2817 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 2213 2304 2423 3452 1870
Size: 343 91 119 1029 4545
Fragments arranged by size:
4545 1029 343 119 91
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2641 3935 276
Size: 53 83 186 936 1107 1294 2468
Fragments arranged by size:
2468 1294 1107 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SexAI SfoI SrfI SwaI XcmI