Restriction map for
pFN21AB5810
(Circular) (Six-base) MAPSORT of: pFN21AB5810.seq Check: 6040 from: 1 to: 6049
pFN21AB5810 6049 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2413 2813 3392 1937
Size: 476 400 579 4594
Fragments arranged by size:
4594 579 476 400
Acc65I G'GTAC_C
Cuts at: 2274 3370 2274
Size: 1096 4953
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 2786 3255 4112 5496 5764 1632
Size: 466 688 469 857 1384 268 1917
Fragments arranged by size:
1917 1384 857 688 469 466 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 3099 4948 5925 1203
Size: 1896 1849 977 1327
Fragments arranged by size:
1896 1849 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 2295 2932 3792 5364 2295
Size: 637 860 1572 2980
Fragments arranged by size:
2980 1572 860 637
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 2614 2663 2799 3063 3186 3229 3358 3584
Size: 1121 49 136 264 123 43 129 226
Cuts at: 3584 3736 5845 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1121 264 226 152 136 129
123 49 43
AseI AT'TA_AT
Cuts at: 160 1032 2379 4603 160
Size: 872 1347 2224 1606
Fragments arranged by size:
2224 1606 1347 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 3374 1870
Size: 73 286 1145 4545
Fragments arranged by size:
4545 1145 286 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 3370 4825 618
Size: 325 217 627 487 1096 1455 1842
Fragments arranged by size:
1842 1455 1096 627 487 325 217
BanII G_rGCy'C
Cuts at: 1391 3368 1391
Size: 1977 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2067 928
Size: 971 168 4910
Fragments arranged by size:
4910 971 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3111 3130 6044 3111
Size: 19 2914 3116
Fragments arranged by size:
3116 2914 19
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 4110 5266 1790
Size: 2320 1156 2573
Fragments arranged by size:
2573 2320 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 4738 442
Size: 416 53 83 399 24 2363 958 1753
Fragments arranged by size:
2363 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2402 3245 4709 1239
Size: 833 330 843 1464 2579
Fragments arranged by size:
2579 1464 843 833 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 4174 5039 5337 3029
Size: 1145 865 298 3741
Fragments arranged by size:
3741 1145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2988 4712 882
Size: 617 1489 1724 2219
Fragments arranged by size:
2219 1724 1489 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3856 4238 4873 275
Size: 53 83 186 936 2323 382 635 1451
Fragments arranged by size:
2323 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5824
Fragments arranged by size:
5824 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6049
BseYI C'CCAG_C
Cuts at: 2596 3086 3302 5252 5748 2596
Size: 490 216 1950 496 2897
Fragments arranged by size:
2897 1950 496 490 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3177 1163
Size: 573 1441 4035
Fragments arranged by size:
4035 1441 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2180 3285 4110 4195 5266 2180
Size: 1105 825 85 1071 2963
Fragments arranged by size:
2963 1105 1071 825 85
BsmI GAATG_Cn'
Cuts at: 2507 3560 3653 2507
Size: 1053 93 4903
Fragments arranged by size:
4903 1053 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 3285 3368 4110 4195 5266 1391
Size: 399 390 1105 83 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 399 390 85 83
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3936 1394
Size: 429 2113 3507
Fragments arranged by size:
3507 2113 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3044 4544 4718 65
Size: 1183 1796 1500 174 1396
Fragments arranged by size:
1796 1500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 3910
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 3341 4109 5121 3341
Size: 768 1012 4269
Fragments arranged by size:
4269 1012 768
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 2492 4853 2492
Size: 2361 3688
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3310
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2553 3111 3130 3379 3746 4131 4148 5600
Size: 581 558 19 249 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 581 558 385 367 346 249
86 19 17 12
BstZ17I GTA'TAC
Cuts at: 2814 2814
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2728 487
Size: 683 56 1502 3808
Fragments arranged by size:
3808 1502 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2151 3565 4359 4379 2061
Size: 90 1414 794 20 3731
Fragments arranged by size:
3731 1414 794 90 20
ClaI AT'CG_AT
Cuts at: 1554 2398 1554
Size: 844 5205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2685 2685
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2633 5056 809
Size: 507 1317 2423 1802
Fragments arranged by size:
2423 1802 1317 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2676 3496 4385
Size: 54 1425 50 275 18 846 820 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 846 820 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3979 1353
Size: 2626 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2098 2402 2786 3245 3255 4112
Size: 393 440 26 304 384 459 10 857
Cuts at: 4112 4709 5496 5764 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 857 787 597 459 440 393 384
304 268 26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2765
Size: 6049
EcoO109I rG'GnC_Cy
Cuts at: 3004 3004
Size: 6049
EcoRI G'AATT_C
Cuts at: 2614 3229 3358 3736 2614
Size: 615 129 378 4927
Fragments arranged by size:
4927 615 378 129
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2295 5196 1678
Size: 617 2901 2531
Fragments arranged by size:
2901 2531 617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2252 2284 4690
Size: 32 135 32 440 32 208 32 2406
Cuts at: 4690 4722 4764 4796 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2406 440 208 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2414 3393 3639 669
Size: 988 281 476 979 246 3079
Fragments arranged by size:
3079 988 979 476 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 2278 3374 2278
Size: 1096 4953
MfeI C'AATT_G
Cuts at: 2163 3648 2163
Size: 1485 4564
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 2662 5163 5347 1305
Size: 1209 148 2501 184 2007
Fragments arranged by size:
2501 2007 1209 184 148
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2678 10
Size: 54 1425 343 846 3381
Fragments arranged by size:
3381 1425 846 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4007 4366 4525 518
Size: 648 2841 359 159 2042
Fragments arranged by size:
2841 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2528 2596 3465 4142 5290 5535 1606
Size: 922 68 869 677 1148 245 2120
Fragments arranged by size:
2120 1148 922 869 677 245 68
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5497
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2086 2112 3103 3407 4952 1132
Size: 97 857 26 991 304 1545 2229
Fragments arranged by size:
2229 1545 991 857 304 97 26
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 3099 4948 3099
Size: 1849 4200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6049
PfoI T'CCnGG_A
Cuts at: 2049 2088 3190 2049
Size: 39 1102 4908
Fragments arranged by size:
4908 1102 39
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3004
Size: 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspXI vC'TCGA_Gb
Cuts at: 2229 2229
Size: 6049
PstI C_TGCA'G
Cuts at: 830 3401 830
Size: 2571 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2528 2596 2528
Size: 68 5981
SalI G'TCGA_C
Cuts at: 1936 2412 3391 1936
Size: 476 979 4594
Fragments arranged by size:
4594 979 476
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 2807 2807
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 3397 4532 5213 5404 826
Size: 219 2352 1135 681 191 1471
Fragments arranged by size:
2352 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 3008 4153 5054 5316 820
Size: 197 926 286 779 1145 901 262 1553
Fragments arranged by size:
1553 1145 926 901 779 286 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 2086 2112 3407 1132
Size: 954 26 1295 3774
Fragments arranged by size:
3774 1295 954 26
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 2964 1517
Size: 1447 4602
StyI C'CwwG_G
Cuts at: 513 1065 3488 513
Size: 552 2423 3074
Fragments arranged by size:
3074 2423 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3316 4076 4093 4246 4431 4876 325
Size: 1255 1736 760 17 153 185 445 1498
Fragments arranged by size:
1736 1498 1255 760 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 4295 6007
Size: 274 80 33 51 1443 258 2060 1712
Cuts at: 6007 96
Size: 138
Fragments arranged by size:
2060 1712 1443 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 6009 6041 2053
Size: 32 3924 32 2061
Fragments arranged by size:
3924 2061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 2229 1943
Size: 286 5763
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI