Restriction map for
pFN21AB5802
(Circular) (Six-base) MAPSORT of: pFN21AB5802.seq Check: 2102 from: 1 to: 6118
pFN21AB5802 6118 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3141 3461 1937
Size: 208 996 320 4594
Fragments arranged by size:
4594 996 320 208
Acc65I G'GTAC_C
Cuts at: 2418 3439 2418
Size: 1021 5097
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2859 3105 3384 4181 5565 5833 1632
Size: 1227 246 279 797 1384 268 1917
Fragments arranged by size:
1917 1384 1227 797 279 268 246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 2421 4949 2421
Size: 2528 3590
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 2194 1166
Size: 1028 5090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 3861 5433 3861
Size: 1572 4546
ApaI G_GGCC'C
Cuts at: 3085 3085
Size: 6118
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 3295 3427 3653 3805 5914 1493
Size: 1802 132 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152 132
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2418 3012 3439 4894
Size: 325 217 627 394 237 594 427 1455
Cuts at: 4894 618
Size: 1842
Fragments arranged by size:
1842 1455 627 594 427 394 325 237
217
BanII G_rGCy'C
Cuts at: 1391 2042 3085 3437 1391
Size: 651 1043 352 4072
Fragments arranged by size:
4072 1043 651 352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2865 2980 928
Size: 971 966 115 4066
Fragments arranged by size:
4066 971 966 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 2757 1548
Size: 1209 4909
BfrBI ATG'CAT
Cuts at: 2129 2129
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 3017 3085 4179 5335 1790
Size: 1227 68 1094 1156 2573
Fragments arranged by size:
2573 1227 1156 1094 68
BmgBI CAC'GTC
Cuts at: 1318 2470 1318
Size: 1152 4966
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2201 2551 2771 4778 1239
Size: 888 74 350 220 2007 2579
Fragments arranged by size:
2579 2007 888 350 220 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4243 5108 5406 4243
Size: 865 298 4955
Fragments arranged by size:
4955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2421 4551 4949 5223 5370 5781 2421
Size: 2130 398 274 147 411 2758
Fragments arranged by size:
2758 2130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2199 2229 2900 2930 1178
Size: 30 165 30 796 30 671 30 4366
Fragments arranged by size:
4366 796 671 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6118
BseYI C'CCAG_C
Cuts at: 2371 2977 5321 5817 2371
Size: 606 2344 496 2672
Fragments arranged by size:
2672 2344 606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2469 1163
Size: 573 733 4812
Fragments arranged by size:
4812 733 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2897 3568 4329 5357 1540
Size: 275 186 896 671 761 1028 2301
Fragments arranged by size:
2301 1028 896 761 671 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2475 4179 4264 5335 2042
Size: 433 1704 85 1071 2825
Fragments arranged by size:
2825 1704 1071 433 85
BsmI GAATG_Cn'
Cuts at: 2329 3358 3629 3722 2329
Size: 1029 271 93 4725
Fragments arranged by size:
4725 1029 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2475 3017 3085 3437 4179 4264
Size: 399 252 433 542 68 352 742 85
Cuts at: 4264 5335 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 542 433 399 352 252
85 68
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2592 2959 3070 3218 4613 4787 65
Size: 1183 1344 367 111 148 1395 174 1396
Fragments arranged by size:
1396 1395 1344 1183 367 174 148 111
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 3247 4762 4949 1700
Size: 110 611 826 1515 187 2869
Fragments arranged by size:
2869 1515 826 611 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 2660 4922 2660
Size: 2262 3856
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113
Size: 1476 367 385 17 1452 86 12 346
Cuts at: 6113 1972
Size: 1977
Fragments arranged by size:
1977 1476 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 4150
Fragments arranged by size:
4150 939 552 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 3634 4428 4448 2304
Size: 1330 794 20 3974
Fragments arranged by size:
3974 1330 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 2941 3166 5125 809
Size: 507 1209 416 225 1959 1802
Fragments arranged by size:
1959 1802 1209 507 416 225
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3039 3565 4454
Size: 54 1425 50 275 18 1209 526 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1209 889 526 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 2891 3124 3360 4048 1353
Size: 1127 411 233 236 688 3423
Fragments arranged by size:
3423 1127 688 411 236 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2040 2040
Size: 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2201 2551 2771 2859 3105 3384
Size: 393 495 74 350 220 88 246 279
Cuts at: 3384 4181 4778 5565 5833 1239
Size: 797 597 787 268 1524
Fragments arranged by size:
1524 797 787 597 495 393 350 279
268 246 220 88 74
EcoO109I rG'GnC_Cy
Cuts at: 2916 3081 3082 2916
Size: 165 1 5952
Fragments arranged by size:
5952 165 1
EcoRI G'AATT_C
Cuts at: 3295 3427 3805 3295
Size: 132 378 5608
Fragments arranged by size:
5608 378 132
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2900 2932 3097
Size: 32 135 32 515 32 781 32 165
Cuts at: 3097 3129 4759 4791 4833 4865 1373
Size: 32 1630 32 42 32 2626
Fragments arranged by size:
2626 1630 781 515 165 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2403 3130 3462 3708 669
Size: 988 281 208 257 727 332 246 3079
Fragments arranged by size:
3079 988 727 332 281 257 246 208
HindIII A'AGCT_T
Cuts at: 748 2441 748
Size: 1693 4425
HpaI GTT'AAC
Cuts at: 2403 3708 2403
Size: 1305 4813
KpnI G_GTAC'C
Cuts at: 2422 3443 2422
Size: 1021 5097
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2194 2297 2390 2753 4076 4435 4594
Size: 648 1028 103 93 363 1323 359 159
Cuts at: 4594 518
Size: 2042
Fragments arranged by size:
2042 1323 1028 648 363 359 159 103
93
MspA1I CmG'CkG
Cuts at: 1606 2893 3534 4211 5359 5604 1606
Size: 1287 641 677 1148 245 2120
Fragments arranged by size:
2120 1287 1148 677 641 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NsiI A_TGCA'T
Cuts at: 2131 2131
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2970 3476 5021 1132
Size: 97 1741 506 1545 2229
Fragments arranged by size:
2229 1741 1545 506 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2642 1108
Size: 1534 4584
PfoI T'CCnGG_A
Cuts at: 2217 2217
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2916 2916
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 3081 3081
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2322 3470 830
Size: 1492 1148 3478
Fragments arranged by size:
3478 1492 1148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
SacI G_AGCT'C
Cuts at: 2042 2042
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 2144 3460 1936
Size: 208 1316 4594
Fragments arranged by size:
4594 1316 208
SapI GCTCTTCn'nnn_
Cuts at: 2480 2891 2480
Size: 411 5707
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2086 2318 2648 3163 3466 4601 5282
Size: 219 1041 232 330 515 303 1135 681
Cuts at: 5282 5473 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1041 681 515 330 303 232
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4222 5123 5385 820
Size: 197 926 2279 901 262 1553
Fragments arranged by size:
2279 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 3136 152
Size: 2984 3134
SphI G_CATG'C
Cuts at: 1132 2970 3476 1132
Size: 1838 506 3774
Fragments arranged by size:
3774 1838 506
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2004 3557 513
Size: 552 939 1553 3074
Fragments arranged by size:
3074 1553 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 4162 4315 4500 4945 325
Size: 1255 2565 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3198 4364 6076
Size: 274 80 33 51 1443 1221 1166 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
1712 1443 1221 1166 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI