Restriction map for
pFN21AB5801
(Circular) (Six-base) MAPSORT of: pFN21AB5801.seq Check: 4621 from: 1 to: 5818
pFN21AB5801 5818 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2619 4717 5694 1203
Size: 1416 2098 977 1327
Fragments arranged by size:
2098 1416 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2997 3561 5133 2997
Size: 564 1572 3682
Fragments arranged by size:
3682 1572 564
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2060 2293 2341 3101 3127 3353 3505 5614
Size: 567 233 48 760 26 226 152 2109
Cuts at: 5614 1493
Size: 1697
Fragments arranged by size:
2109 1697 760 567 233 226 152 48
26
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2497 2567 2775 3143 1870
Size: 73 554 70 208 368 4545
Fragments arranged by size:
4545 554 368 208 73 70
BamHI G'GATC_C
Cuts at: 2562 2947 3148 3515 2562
Size: 385 201 367 4865
Fragments arranged by size:
4865 385 367 201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3139 4594 618
Size: 325 217 627 1352 1455 1842
Fragments arranged by size:
1842 1455 1352 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2536 2807 3137 1391
Size: 1145 271 330 4072
Fragments arranged by size:
4072 1145 330 271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 2645 2837 928
Size: 971 547 199 192 3909
Fragments arranged by size:
3909 971 547 199 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2147 2181 2702 2736 2964 2998 4009
Size: 34 210 34 521 34 228 34 1011
Cuts at: 4009 4043 1903
Size: 34 3678
Fragments arranged by size:
3678 1011 521 228 210 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4429 136
Size: 107 122 71 631 605 2757 1525
Fragments arranged by size:
2757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2032 5813 2032
Size: 3781 2037
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 3879 5035 1790
Size: 2089 1156 2573
Fragments arranged by size:
2573 2089 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2720 2810 3036
Size: 416 53 83 399 24 1303 90 226
Cuts at: 3036 3549 4507 442
Size: 513 958 1753
Fragments arranged by size:
1753 1303 958 513 416 399 226 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2674 4478 1239
Size: 1435 1804 2579
Fragments arranged by size:
2579 1804 1435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2470 4481 882
Size: 617 971 2011 2219
Fragments arranged by size:
2219 2011 971 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3625 4007 4642 275
Size: 53 83 186 936 2092 382 635 1451
Fragments arranged by size:
2092 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 2521 2551 1178
Size: 30 165 30 1030 30 58 30 4445
Fragments arranged by size:
4445 1030 165 58 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2612 2615 2759 2762 1186
Size: 1423 3 3 144 3 4242
Fragments arranged by size:
4242 1423 144 3 3 3
BseYI C'CCAG_C
Cuts at: 2871 5021 5517 2871
Size: 2150 496 3172
Fragments arranged by size:
3172 2150 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 1163
Size: 573 479 4766
Fragments arranged by size:
4766 573 479
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2536 3879 3964 5035 2536
Size: 1343 85 1071 3319
Fragments arranged by size:
3319 1343 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2536 2807 3137 3879 3964 5035 1391
Size: 399 746 271 330 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 399 330 271 85
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2679 3173 844
Size: 1835 494 3489
Fragments arranged by size:
3489 1835 494
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 2959 4313 4487 65
Size: 1183 1143 568 1354 174 1396
Fragments arranged by size:
1396 1354 1183 1143 568 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3000 4462 4649 1700
Size: 110 1190 1462 187 2869
Fragments arranged by size:
2869 1462 1190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 2149 3878 4890 2149
Size: 1729 1012 3077
Fragments arranged by size:
3077 1729 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 2390 2997 794
Size: 1399 197 607 3615
Fragments arranged by size:
3615 1399 607 197
BstBI TT'CG_AA
Cuts at: 2908 4622 2908
Size: 1714 4104
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2871 2894 2871
Size: 23 5795
BstYI r'GATC_y
Cuts at: 1972 2032 2562 2947 3148 3515 3900 3917 5369
Size: 60 530 385 201 367 385 17 1452
Cuts at: 5369 5455 5467 5813 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 530 385 385 367 346 201
86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2434 2589 2643 513
Size: 552 939 81 349 155 54 3688
Fragments arranged by size:
3688 939 552 349 155 81 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2237 487
Size: 683 56 1011 4068
Fragments arranged by size:
4068 1011 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 2364 3122 2364
Size: 758 5060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3265 4154 5783
Size: 54 1425 50 275 18 1435 889 1629
Cuts at: 5783 8
Size: 43
Fragments arranged by size:
1629 1435 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 2919 3748 1353
Size: 1248 318 829 3423
Fragments arranged by size:
3423 1248 829 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2534 2534
Size: 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2674 3881 4478 5265 5533 1239
Size: 393 1042 1207 597 787 268 1524
Fragments arranged by size:
1524 1207 1042 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2973 3095 2973
Size: 122 5696
EcoO109I rG'GnC_Cy
Cuts at: 2181 2556 2571 3031 2181
Size: 375 15 460 4968
Fragments arranged by size:
4968 460 375 15
EcoRI G'AATT_C
Cuts at: 2293 3127 3505 2293
Size: 834 378 4606
Fragments arranged by size:
4606 834 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2405 2068
Size: 337 5481
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 4965 1678
Size: 3287 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2433 2453 2465
Size: 32 135 32 725 32 104 20 12
Cuts at: 2465 2485 2521 2553 4459 4491 4533 4565 1373
Size: 20 36 32 1906 32 42 32 2626
Fragments arranged by size:
2626 1906 725 135 104 42 36 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2416 748
Size: 1668 4150
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 2484 2733 4932 5116 1305
Size: 1029 150 249 2199 184 2007
Fragments arranged by size:
2199 2007 1029 249 184 150
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3996
Fragments arranged by size:
3996 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3776 4135 4294 518
Size: 648 1458 1152 359 159 2042
Fragments arranged by size:
2042 1458 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3234 3911 5059 5304 1606
Size: 1628 677 1148 245 2120
Fragments arranged by size:
2120 1628 1148 677 245
NaeI GCC'GGC
Cuts at: 3002 3002
Size: 5818
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 2589 513
Size: 552 939 81 504 3742
Fragments arranged by size:
3742 939 552 504 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 3000 3000
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 2623 3077 3176 4721 1132
Size: 97 1394 454 99 1545 2229
Fragments arranged by size:
2229 1545 1394 454 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 2619 4717 2619
Size: 2098 3720
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5818
PfoI T'CCnGG_A
Cuts at: 2510 2558 2510
Size: 48 5770
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2556 2571 3031 2181
Size: 375 15 460 4968
Fragments arranged by size:
4968 460 375 15
PshAI GACnn'nnGTC
Cuts at: 1613 3029 1613
Size: 1416 4402
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2160 2768 2858 2993 3170 830
Size: 1330 608 90 135 177 3478
Fragments arranged by size:
3478 1330 608 177 135 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
SacI G_AGCT'C
Cuts at: 2536 2536
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SanDI GG'GwC_CC
Cuts at: 2571 2571
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SexAI A'CCwGG_T
Cuts at: 2546 2546
Size: 5818
SfcI C'TryA_G
Cuts at: 826 1045 2156 2176 2764 2854 2989 3166 4301
Size: 219 1111 20 588 90 135 177 1135
Cuts at: 4301 4982 5173 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 588 219 191 177
135 90 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 2499 3145 1872
Size: 627 646 4545
Fragments arranged by size:
4545 646 627
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2775 3922 4823 5085 820
Size: 197 926 832 1147 901 262 1553
Fragments arranged by size:
1553 1147 926 901 832 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3077 3176 1132
Size: 1945 99 3774
Fragments arranged by size:
3774 1945 99
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2589 3257 513
Size: 552 939 81 504 668 3074
Fragments arranged by size:
3074 939 668 552 504 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 4064 5776
Size: 274 80 33 51 1443 639 1448 1712
Cuts at: 5776 96
Size: 138
Fragments arranged by size:
1712 1448 1443 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5778 5810 5778
Size: 32 5786
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 2775 1943
Size: 832 4986
XmaI C'CCGG_G
Cuts at: 1870 2497 3143 1870
Size: 627 646 4545
Fragments arranged by size:
4545 646 627
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SapI SfiI SfoI SrfI SwaI XcmI