Restriction map for
pFN21AB5799
(Circular) (Six-base) MAPSORT of: pFN21AB5799.seq Check: 5902 from: 1 to: 7207
pFN21AB5799 7207 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2874 3597 5017 278
Size: 53 83 186 936 1338 723 1420 2468
Fragments arranged by size:
2468 1420 1338 936 723 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3888 4550 1937
Size: 1951 662 4594
Fragments arranged by size:
4594 1951 662
Acc65I G'GTAC_C
Cuts at: 4528 4528
Size: 7207
AclI AA'CG_TT
Cuts at: 1563 4461 5334 5707 1563
Size: 2898 873 373 3063
Fragments arranged by size:
3063 2898 873 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2768 5270 6654 6922 1632
Size: 953 183 2502 1384 268 1917
Fragments arranged by size:
2502 1917 1384 953 268 183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7010
AflIII A'CryG_T
Cuts at: 1203 2828 6106 7083 1203
Size: 1625 3278 977 1327
Fragments arranged by size:
3278 1625 1327 977
AgeI A'CCGG_T
Cuts at: 6038 6038
Size: 7207
AhdI GACnn_n'nnGTC
Cuts at: 5936 5936
Size: 7207
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3872 1184
Size: 2688 4519
AlwNI CAG_nnn'CTG
Cuts at: 2902 2920 3000 3541 4003 4950 6522 2902
Size: 18 80 541 462 947 1572 3587
Fragments arranged by size:
3587 1572 947 541 462 80 18
ApaI G_GGCC'C
Cuts at: 2102 4170 2102
Size: 2068 5139
ApaLI G'TGCA_C
Cuts at: 5264 6420 5264
Size: 1156 6051
ApoI r'AATT_y
Cuts at: 1493 2173 2642 3906 4019 4516 4742 4894 7003
Size: 680 469 1264 113 497 226 152 2109
Cuts at: 7003 1493
Size: 1697
Fragments arranged by size:
2109 1697 1264 680 497 469 226 152
113
AseI AT'TA_AT
Cuts at: 160 1032 3393 5761 160
Size: 872 2361 2368 1606
Fragments arranged by size:
2368 2361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
AvaI C'yCGr_G
Cuts at: 1870 1943 3564 3621 4532 1870
Size: 73 1621 57 911 4545
Fragments arranged by size:
4545 1621 911 73 57
BamHI G'GATC_C
Cuts at: 4537 4904 4537
Size: 367 6840
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4528 5983 618
Size: 325 217 627 2741 1455 1842
Fragments arranged by size:
2741 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2760 2856 4053 4170 4526 1391
Size: 711 658 96 1197 117 356 4072
Fragments arranged by size:
4072 1197 711 658 356 117 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 3235 4213 928
Size: 971 711 625 978 3922
Fragments arranged by size:
3922 978 971 711 625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4229 4263 5398 5432 1903
Size: 34 2292 34 1135 34 3678
Fragments arranged by size:
3678 2292 1135 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2174 5099 6309 2174
Size: 2925 1210 3072
Fragments arranged by size:
3072 2925 1210
BclI T'GATC_A
Cuts at: 1548 3825 1548
Size: 2277 4930
BfrBI ATG'CAT
Cuts at: 3467 3467
Size: 7207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 5818 136
Size: 107 122 71 631 605 2493 1653 1525
Fragments arranged by size:
2493 1653 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7202 7202
Size: 7207
BlpI GC'TnA_GC
Cuts at: 2759 4624 2759
Size: 1865 5342
Bme1580I G_kGCm'C
Cuts at: 1790 2102 3034 3448 3715 3729 4170 5268 6424
Size: 312 932 414 267 14 441 1098 1156
Cuts at: 6424 1790
Size: 2573
Fragments arranged by size:
2573 1156 1098 932 441 414 312 267
14
BmgBI CAC'GTC
Cuts at: 1318 2831 1318
Size: 1513 5694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 2290 3240
Size: 416 53 83 399 24 667 206 950
Cuts at: 3240 4938 5896 442
Size: 1698 958 1753
Fragments arranged by size:
1753 1698 958 950 667 416 399 206
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2730 2730
Size: 7207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2444 3299 5867 1239
Size: 1013 192 855 2568 2579
Fragments arranged by size:
2579 2568 1013 855 192
Bpu10I CC'TnA_GC
Cuts at: 2813 2813
Size: 7207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 3622 5332 6197 6495 2243
Size: 1379 1710 865 298 2955
Fragments arranged by size:
2955 1710 1379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2044 4187 4330 5870 882
Size: 617 545 2143 143 1540 2219
Fragments arranged by size:
2219 2143 1540 617 545 143
BsaAI yAC'GTr
Cuts at: 493 1204 4091 4998 493
Size: 711 2887 907 2702
Fragments arranged by size:
2887 2702 907 711
BsaBI GATnn'nnATC
Cuts at: 7201 7201
Size: 7207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2475 2871 3594 3891
Size: 53 83 186 936 942 396 723 297
Cuts at: 3891 5014 5396 6031 275
Size: 1123 382 635 1451
Fragments arranged by size:
1451 1123 942 936 723 635 396 382
297 186 83 53
BsaWI w'CCGG_w
Cuts at: 5640 6038 6312 6459 6870 5640
Size: 398 274 147 411 5977
Fragments arranged by size:
5977 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2427 2457 3566 3596 1178
Size: 30 165 30 1024 30 1109 30 4789
Fragments arranged by size:
4789 1109 1024 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2129 2132 2144 2147 2150 2153 2525
Size: 870 73 3 12 3 3 3 372
Cuts at: 2525 3437 3881 1186
Size: 912 444 4512
Fragments arranged by size:
4512 912 870 444 372 73 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2078 3472 4286 6410 6906 2078
Size: 1394 814 2124 496 2379
Fragments arranged by size:
2379 2124 1394 814 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3101 3416 3467 3978 4196 1163
Size: 573 1365 315 51 511 218 4174
Fragments arranged by size:
4174 1365 573 511 315 218 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4657 5418 6446 1540
Size: 275 186 2656 761 1028 2301
Fragments arranged by size:
2656 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2551 2856 4074 5268 5353 6424 2370
Size: 181 305 1218 1194 85 1071 3153
Fragments arranged by size:
3153 1218 1194 1071 305 181 85
BsmI GAATG_Cn'
Cuts at: 4718 4811 4718
Size: 93 7114
BsmBI CGTCTCn'nnnn_
Cuts at: 3500 3500
Size: 7207
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2370 2551 2760 2856 3034 3448
Size: 399 312 268 181 209 96 178 414
Cuts at: 3448 3715 3729 4053 4074 4170 4526 5268 5353
Size: 267 14 324 21 96 356 742 85
Cuts at: 5353 6424 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 356 324 312
268 267 209 181 178 96 96 85
21 14
BspEI T'CCGG_A
Cuts at: 6870 6870
Size: 7207
BspHI T'CATG_A
Cuts at: 2839 3948 4467 5096 6826 2839
Size: 1109 519 629 1730 3220
Fragments arranged by size:
3220 1730 1109 629 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2995 3123 3489 4562 844
Size: 2151 128 366 1073 3489
Fragments arranged by size:
3489 2151 1073 366 128
BsrBI CCG'CTC
Cuts at: 1394 1823 3057 5094 1394
Size: 429 1234 2037 3507
Fragments arranged by size:
3507 2037 1234 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 3090 3467 5702 5876 65
Size: 1183 942 900 377 2235 174 1396
Fragments arranged by size:
2235 1396 1183 942 900 377 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5851 6038 1700
Size: 110 4041 187 2869
Fragments arranged by size:
4041 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2727 3024 96
Size: 2631 297 4279
Fragments arranged by size:
4279 2631 297
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7207
BssSI C'ACGA_G
Cuts at: 2249 3546 4311 5267 6279 2249
Size: 1297 765 956 1012 3177
Fragments arranged by size:
3177 1297 1012 956 765
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7207
BstBI TT'CG_AA
Cuts at: 6011 6011
Size: 7207
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7207
BstXI CCAn_nnnn'nTGG
Cuts at: 3460 4042 3460
Size: 582 6625
BstYI r'GATC_y
Cuts at: 1972 2151 3202 3492 4441 4537 4904 5289 5306
Size: 179 1051 290 949 96 367 385 17
Cuts at: 5306 6758 6844 6856 7202 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1051 949 385 367 346 290
179 96 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2014 3041 2014
Size: 1027 6180
BtgI C'CryG_G
Cuts at: 513 1065 2004 3366 4188 513
Size: 552 939 1362 822 3532
Fragments arranged by size:
3532 1362 939 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6468
Fragments arranged by size:
6468 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 3484 3984 3990 4348 4723 5517 5537 3316
Size: 168 500 6 358 375 794 20 4986
Fragments arranged by size:
4986 794 500 375 358 168 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7172
DraI TTT'AAA
Cuts at: 4511 4511
Size: 7207
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2612 6214 809
Size: 507 1296 3602 1802
Fragments arranged by size:
3602 1802 1296 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2736 4062
Size: 54 1425 50 275 18 315 591 1326
Cuts at: 4062 4119 4654 5543 7172 8
Size: 57 535 889 1629 43
Fragments arranged by size:
1629 1425 1326 889 591 535 315 275
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4654 1537
Size: 275 2842 4090
Fragments arranged by size:
4090 2842 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2507 2515 3791 5137 1353
Size: 1154 8 1276 1346 3423
Fragments arranged by size:
3423 1346 1276 1154 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5794 6168 6314 387
Size: 1064 138 4205 374 146 1280
Fragments arranged by size:
4205 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 7207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2444 2585 2768 3299 5270 5867
Size: 393 620 192 141 183 531 1971 597
Cuts at: 5867 6654 6922 1239
Size: 787 268 1524
Fragments arranged by size:
1971 1524 787 620 597 531 393 268
192 183 141
EcoNI CCTnn'n_nnAGG
Cuts at: 3257 3812 3962 3257
Size: 555 150 6502
Fragments arranged by size:
6502 555 150
EcoO109I rG'GnC_Cy
Cuts at: 2099 3478 4166 4167 2099
Size: 1379 688 1 5139
Fragments arranged by size:
5139 1379 688 1
EcoRI G'AATT_C
Cuts at: 2173 3906 4516 4894 2173
Size: 1733 610 378 4486
Fragments arranged by size:
4486 1733 610 378
FspI TGC'GCA
Cuts at: 5713 5713
Size: 7207
HaeII r_GCGC'y
Cuts at: 1678 6354 1678
Size: 4676 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 2908 2940 3540
Size: 32 135 32 804 32 500 32 600
Cuts at: 3540 3566 3572 3598 5848 5880 5922 5954 1373
Size: 26 6 26 2250 32 42 32 2626
Fragments arranged by size:
2626 2250 804 600 500 135 42 32
32 32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 4551 4797 669
Size: 988 281 714 1899 246 3079
Fragments arranged by size:
3079 1899 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 2261 2351 4301 748
Size: 1513 90 1950 3654
Fragments arranged by size:
3654 1950 1513 90
HpaI GTT'AAC
Cuts at: 4797 4797
Size: 7207
KpnI G_GTAC'C
Cuts at: 4532 4532
Size: 7207
MfeI C'AATT_G
Cuts at: 4806 4806
Size: 7207
MluI A'CGCG_T
Cuts at: 7083 7083
Size: 7207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2858 4099 6321 6505 1305
Size: 1553 1241 2222 184 2007
Fragments arranged by size:
2222 2007 1553 1241 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 4121 10
Size: 54 1425 343 906 1383 3096
Fragments arranged by size:
3096 1425 1383 906 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 3833 3947 4115 5165 5524 5683
Size: 648 994 1673 114 168 1050 359 159
Cuts at: 5683 518
Size: 2042
Fragments arranged by size:
2042 1673 1050 994 648 359 168 159
114
MspA1I CmG'CkG
Cuts at: 1606 3554 4067 4277 4623 5300 6448 6693 1606
Size: 1948 513 210 346 677 1148 245 2120
Fragments arranged by size:
2120 1948 1148 677 513 346 245 210
NcoI C'CATG_G
Cuts at: 513 1065 2004 3366 4188 513
Size: 552 939 1362 822 3532
Fragments arranged by size:
3532 1362 939 822 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7207
NotI GC'GGCC_GC
Cuts at: 4654 4654
Size: 7207
NsiI A_TGCA'T
Cuts at: 3469 3469
Size: 7207
NspI r_CATG'y
Cuts at: 1132 1229 4104 4565 6110 1132
Size: 97 2875 461 1545 2229
Fragments arranged by size:
2875 2229 1545 461 97
PasI CC'CwG_GG
Cuts at: 1348 2103 3034 1348
Size: 755 931 5521
Fragments arranged by size:
5521 931 755
PciI A'CATG_T
Cuts at: 6106 6106
Size: 7207
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3541 1108
Size: 1794 639 4774
Fragments arranged by size:
4774 1794 639
PfoI T'CCnGG_A
Cuts at: 3495 3495
Size: 7207
PmeI GTTT'AAAC
Cuts at: 4511 4511
Size: 7207
PmlI CAC'GTG
Cuts at: 4091 4091
Size: 7207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5276 5308 6789 6821 5276
Size: 32 1481 32 5662
Fragments arranged by size:
5662 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3478 3478
Size: 7207
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7207
PsiI TTA'TAA
Cuts at: 4777 4777
Size: 7207
PspOMI G'GGCC_C
Cuts at: 2098 4166 2098
Size: 2068 5139
PstI C_TGCA'G
Cuts at: 830 4559 830
Size: 3729 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7207
PvuII CAG'CTG
Cuts at: 3554 4277 3554
Size: 723 6484
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 7207
SalI G'TCGA_C
Cuts at: 1936 4549 1936
Size: 2613 4594
SapI GCTCTTCn'nnn_
Cuts at: 3791 3791
Size: 7207
SbfI CC_TGCA'GG
Cuts at: 4559 4559
Size: 7207
ScaI AGT'ACT
Cuts at: 5455 7167 5455
Size: 1712 5495
SexAI A'CCwGG_T
Cuts at: 3963 3963
Size: 7207
SfcI C'TryA_G
Cuts at: 826 1045 2693 3527 4555 5690 6371 6562 826
Size: 219 1648 834 1028 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 1028 834 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7207
SmaI CCC'GGG
Cuts at: 1872 3623 4534 1872
Size: 1751 911 4545
Fragments arranged by size:
4545 1751 911
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 3637 5311 6212 6474 820
Size: 197 926 315 1379 1674 901 262 1553
Fragments arranged by size:
1674 1553 1379 926 901 315 262 197
SnaBI TAC'GTA
Cuts at: 493 4998 493
Size: 4505 2702
SpeI A'CTAG_T
Cuts at: 152 2207 152
Size: 2055 5152
SphI G_CATG'C
Cuts at: 1132 4565 1132
Size: 3433 3774
SspI AAT'ATT
Cuts at: 5 52 5131 5
Size: 47 5079 2081
Fragments arranged by size:
5079 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7207
StyI C'CwwG_G
Cuts at: 513 1065 2004 2027 2988 3366 3453 3664 3931
Size: 552 939 23 961 378 87 211 267
Cuts at: 3931 4188 4646 513
Size: 257 458 3074
Fragments arranged by size:
3074 961 939 552 458 378 267 257
211 87 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 5234 5251 5404 5589 6034 325
Size: 1255 482 3172 17 153 185 445 1498
Fragments arranged by size:
3172 1498 1255 482 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2727 3024 5453
Size: 274 80 33 51 1443 750 297 2429
Cuts at: 5453 7165 96
Size: 1712 138
Fragments arranged by size:
2429 1712 1443 750 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7167 7199 7167
Size: 32 7175
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7207
XbaI T'CTAG_A
Cuts at: 4543 4543
Size: 7207
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7207
XmaI C'CCGG_G
Cuts at: 1870 3621 4532 1870
Size: 1751 911 4545
Fragments arranged by size:
4545 1751 911
XmnI GAAnn'nnTTC
Cuts at: 4463 5336 4463
Size: 873 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2872 3595 5015 276
Size: 53 83 186 936 1338 723 1420 2468
Fragments arranged by size:
2468 1420 1338 936 723 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI