Restriction map for
pFN21AB5798
(Circular) (Six-base) MAPSORT of: pFN21AB5798.seq Check: 7664 from: 1 to: 7048
pFN21AB5798 7048 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3397 3397
Size: 7048
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4391 1937
Size: 2454 4594
Acc65I G'GTAC_C
Cuts at: 2978 4369 2978
Size: 1391 5657
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2897 3161 3314 3390 3398 3875 4255
Size: 746 519 264 153 76 8 477 380
Cuts at: 4255 5111 6495 6763 1632
Size: 856 1384 268 1917
Fragments arranged by size:
1917 1384 856 746 519 477 380 268
264 153 76 8
AfeI AGC'GCT
Cuts at: 3375 3375
Size: 7048
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6851
AflIII A'CryG_T
Cuts at: 1203 4283 5947 6924 1203
Size: 3080 1664 977 1327
Fragments arranged by size:
3080 1664 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 4320 5777 4320
Size: 1457 5591
AleI CACnn'nnGTG
Cuts at: 1166 2099 1166
Size: 933 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 2224 2245 2650 3020 3118 3629 4791 6363 2224
Size: 21 405 370 98 511 1162 1572 2909
Fragments arranged by size:
2909 1572 1162 511 405 370 98 21
ApaI G_GGCC'C
Cuts at: 4257 4257
Size: 7048
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 2778 3270 4357 4583 4735 6844 1493
Size: 1285 492 1087 226 152 2109 1697
Fragments arranged by size:
2109 1697 1285 1087 492 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 2444 2665 2707 2835 3008 3094
Size: 73 432 69 221 42 128 173 86
Cuts at: 3094 4373 1870
Size: 1279 4545
Fragments arranged by size:
4545 1279 432 221 173 128 86 73
69 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2970 3003 3492 3525 3904 3937 2970
Size: 33 489 33 379 33 6081
Fragments arranged by size:
6081 489 379 33 33 33
BamHI G'GATC_C
Cuts at: 2121 3999 4378 4745 2121
Size: 1878 379 367 4424
Fragments arranged by size:
4424 1878 379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2660 2761 2978 3024
Size: 325 217 627 266 607 101 217 46
Cuts at: 3024 3066 4369 5824 618
Size: 42 1303 1455 1842
Fragments arranged by size:
1842 1455 1303 627 607 325 266 217
217 101 46 42
BanII G_rGCy'C
Cuts at: 1391 2262 2543 3095 3183 3938 4257 4367 1391
Size: 871 281 552 88 755 319 110 4072
Fragments arranged by size:
4072 871 755 552 319 281 110 88
BbeI G_GCGC'C
Cuts at: 2664 2765 2664
Size: 101 6947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2366 928
Size: 971 467 5610
Fragments arranged by size:
5610 971 467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2671 2705 4111 4145 5239 5273 1903
Size: 34 734 34 1406 34 1094 34 3678
Fragments arranged by size:
3678 1406 1094 734 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3520 4940 6150 3520
Size: 1420 1210 4418
Fragments arranged by size:
4418 1420 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5659 136
Size: 107 122 71 631 605 3987 1525
Fragments arranged by size:
3987 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7043 7043
Size: 7048
BlpI GC'TnA_GC
Cuts at: 4465 4465
Size: 7048
Bme1580I G_kGCm'C
Cuts at: 1790 2058 3027 4257 5109 6265 1790
Size: 268 969 1230 852 1156 2573
Fragments arranged by size:
2573 1230 1156 969 852 268
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 4903
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2963 3976 4078
Size: 416 53 83 399 24 1546 1013 102
Cuts at: 4078 4779 5737 442
Size: 701 958 1753
Fragments arranged by size:
1753 1546 1013 958 701 416 399 102
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2101 2349 3305 3422 3443 3476 3572
Size: 824 38 248 956 117 21 33 96
Cuts at: 3572 5708 1239
Size: 2136 2579
Fragments arranged by size:
2579 2136 956 824 248 117 96 38
33 21
Bpu10I CC'TnA_GC
Cuts at: 2477 3104 3783 2477
Size: 627 679 5742
Fragments arranged by size:
5742 679 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3639 5173 6038 6336 2753
Size: 886 1534 865 298 3465
Fragments arranged by size:
3465 1534 886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2958 5711 882
Size: 617 1459 2753 2219
Fragments arranged by size:
2753 2219 1459 617
BsaAI yAC'GTr
Cuts at: 493 1204 4286 4839 493
Size: 711 3082 553 2702
Fragments arranged by size:
3082 2702 711 553
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2661 2762 4106 4855
Size: 53 83 186 936 1128 101 1344 749
Cuts at: 4855 5237 5872 275
Size: 382 635 1451
Fragments arranged by size:
1451 1344 1128 936 749 635 382 186
101 83 53
BsaWI w'CCGG_w
Cuts at: 2851 2912 5481 5879 6153 6300 6711 2851
Size: 61 2569 398 274 147 411 3188
Fragments arranged by size:
3188 2569 411 398 274 147 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2612 2642 2955 2985 3395
Size: 30 165 30 1209 30 313 30 410
Cuts at: 3395 3425 3554 3584 1178
Size: 30 129 30 4642
Fragments arranged by size:
4642 1209 410 313 165 129 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2270 3257 3332 3353 3642 3671 4299
Size: 874 210 987 75 21 289 29 628
Cuts at: 4299 1186
Size: 3935
Fragments arranged by size:
3935 987 874 628 289 210 75 29
21
BseYI C'CCAG_C
Cuts at: 3111 6251 6747 3111
Size: 3140 496 3412
Fragments arranged by size:
3412 3140 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2225 2455 2701 3410 1163
Size: 573 387 102 230 246 709 4801
Fragments arranged by size:
4801 709 573 387 246 230 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2405 4498 5259 6287 1540
Size: 275 186 404 2093 761 1028 2301
Fragments arranged by size:
2301 2093 1028 761 404 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2262 3216 3716 5109 5194 6265 2115
Size: 147 954 500 1393 85 1071 2898
Fragments arranged by size:
2898 1393 1071 954 500 147 85
BsiWI C'GTAC_G
Cuts at: 2406 2406
Size: 7048
BsmI GAATG_Cn'
Cuts at: 4559 4652 4559
Size: 93 6955
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2115 2262 2543 3027 3095 3183
Size: 399 268 57 147 281 484 68 88
Cuts at: 3183 3216 3716 3938 4257 4367 5109 5194 6265
Size: 33 500 222 319 110 742 85 1071
Cuts at: 6265 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 500 484 399 319 281
268 222 147 110 88 85 68 57
33
BspEI T'CCGG_A
Cuts at: 2851 6711 2851
Size: 3860 3188
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 2709 3136 3397 4403 844
Size: 1365 500 427 261 1006 3489
Fragments arranged by size:
3489 1365 1006 500 427 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 4935 1394
Size: 429 1225 1887 3507
Fragments arranged by size:
3507 1887 1225 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5543 5717 65
Size: 1183 4295 174 1396
Fragments arranged by size:
4295 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2152 5692 5879 1700
Size: 110 342 3540 187 2869
Fragments arranged by size:
3540 2869 342 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7048
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 5108 6120 5108
Size: 1012 6036
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2224 3388 794
Size: 1430 1164 4454
Fragments arranged by size:
4454 1430 1164
BstBI TT'CG_AA
Cuts at: 2691 5852 2691
Size: 3161 3887
BstEII G'GTnAC_C
Cuts at: 1184 2176 2737 2908 1184
Size: 992 561 171 5324
Fragments arranged by size:
5324 992 561 171
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2332 3476 4156 2173
Size: 159 1144 680 5065
Fragments arranged by size:
5065 1144 680 159
BstYI r'GATC_y
Cuts at: 1972 2121 2136 3672 3999 4214 4378 4745 5130
Size: 149 15 1536 327 215 164 367 385
Cuts at: 5130 5147 6599 6685 6697 7043 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1536 1452 385 367 346 327 215
164 149 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 3037 3037
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 4930
Fragments arranged by size:
4930 939 627 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3505 487
Size: 683 56 2279 4030
Fragments arranged by size:
4030 2279 683 56
BtsI GCAGTG_nn'
Cuts at: 3354 4564 5358 5378 3354
Size: 1210 794 20 5024
Fragments arranged by size:
5024 1210 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 4352 4352
Size: 7048
DraIII CAC_nnn'GTG
Cuts at: 3229 3229
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2250 2402
Size: 54 1425 50 275 18 333 87 152
Cuts at: 2402 3955 4495 5384 7013 8
Size: 1553 540 889 1629 43
Fragments arranged by size:
1629 1553 1425 889 540 333 275 152
87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2402 4495 1537
Size: 275 590 2093 4090
Fragments arranged by size:
4090 2093 590 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 3083 3164 3704 3851 4978 1353
Size: 834 896 81 540 147 1127 3423
Fragments arranged by size:
3423 1127 896 834 540 147 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2430 5635 6009 6155 387
Size: 1064 138 841 3205 374 146 1280
Fragments arranged by size:
3205 1280 1064 841 374 146 138
EcoICRI GAG'CTC
Cuts at: 2260 2260
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2101 2349 2378 2897 3161 3305
Size: 393 431 38 248 29 519 264 144
Cuts at: 3305 3314 3390 3398 3422 3443 3476 3572 3875
Size: 9 76 8 24 21 33 96 303
Cuts at: 3875 4255 5111 5708 6495 6763 1239
Size: 380 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 519 431 393 380
303 268 264 248 144 96 76 38
33 29 24 21 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3143 3143
Size: 7048
EcoO109I rG'GnC_Cy
Cuts at: 2221 2506 2685 2885 4254 4340 2221
Size: 285 179 200 1369 86 4929
Fragments arranged by size:
4929 1369 285 200 179 86
EcoRI G'AATT_C
Cuts at: 4357 4735 4357
Size: 378 6670
FspI TGC'GCA
Cuts at: 2615 5554 2615
Size: 2939 4109
HaeII r_GCGC'y
Cuts at: 1678 2245 2585 2664 2765 3136 3377 3783 6195
Size: 567 340 79 101 371 241 406 2412
Cuts at: 6195 1678
Size: 2531
Fragments arranged by size:
2531 2412 567 406 371 340 241 101
79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 3311 3343 3554
Size: 32 135 32 580 32 1127 32 211
Cuts at: 3554 3586 3637 3669 3766 3798 4206 4238 4306
Size: 32 51 32 97 32 408 32 68
Cuts at: 4306 4338 5689 5721 5763 5795 1373
Size: 32 1351 32 42 32 2626
Fragments arranged by size:
2626 1351 1127 580 408 211 135 97
68 51 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2974 4392 4638 669
Size: 988 281 1036 1418 246 3079
Fragments arranged by size:
3079 1418 1036 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7048
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KasI G'GCGC_C
Cuts at: 2660 2761 2660
Size: 101 6947
KpnI G_GTAC'C
Cuts at: 2982 4373 2982
Size: 1391 5657
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3700 3961 4162 6162 6346 1305
Size: 2395 261 201 2000 184 2007
Fragments arranged by size:
2395 2007 2000 261 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 10
Size: 54 1425 343 333 4893
Fragments arranged by size:
4893 1425 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 4166 5006 5365 5524 518
Size: 648 933 2067 840 359 159 2042
Fragments arranged by size:
2067 2042 933 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2633 2807 2946 3154 3283 3385 3918 4239
Size: 1027 174 139 208 129 102 533 321
Cuts at: 4239 4464 5141 6289 6534 1606
Size: 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 1027 677 533 321 245 225
208 174 139 129 102
NarI GG'CG_CC
Cuts at: 2661 2762 2661
Size: 101 6947
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5557
Fragments arranged by size:
5557 939 552
NdeI CA'TA_TG
Cuts at: 387 3496 387
Size: 3109 3939
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 3794 4406 5951 1132
Size: 97 2565 612 1545 2229
Fragments arranged by size:
2565 2229 1545 612 97
PasI CC'CwG_GG
Cuts at: 1348 2332 4277 1348
Size: 984 1945 4119
Fragments arranged by size:
4119 1945 984
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4061 4331 1108
Size: 2953 270 3825
Fragments arranged by size:
3825 2953 270
PfoI T'CCnGG_A
Cuts at: 2117 3420 2117
Size: 1303 5745
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PmlI CAC'GTG
Cuts at: 4286 4286
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 2221
Size: 7048
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 4618 4618
Size: 7048
PspOMI G'GGCC_C
Cuts at: 4253 4253
Size: 7048
PstI C_TGCA'G
Cuts at: 830 2091 2220 2579 2646 3002 3200 3591 4400
Size: 1261 129 359 67 356 198 391 809
Cuts at: 4400 830
Size: 3478
Fragments arranged by size:
3478 1261 809 391 359 356 198 129
67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 2807 3154 3283 3918 4239 2807
Size: 347 129 635 321 5616
Fragments arranged by size:
5616 635 347 321 129
SacI G_AGCT'C
Cuts at: 2262 2262
Size: 7048
SacII CC_GC'GG
Cuts at: 2634 2634
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 3083 3164 3704 3083
Size: 81 540 6427
Fragments arranged by size:
6427 540 81
SbfI CC_TGCA'GG
Cuts at: 2220 2579 4400 2220
Size: 359 1821 4868
Fragments arranged by size:
4868 1821 359
ScaI AGT'ACT
Cuts at: 3722 5296 7008 3722
Size: 1574 1712 3762
Fragments arranged by size:
3762 1712 1574
SfcI C'TryA_G
Cuts at: 826 1045 2087 2216 2575 2642 2748 2998 3196
Size: 219 1042 129 359 67 106 250 198
Cuts at: 3196 3587 4174 4396 5531 6212 6403 826
Size: 391 587 222 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 587 391 359 250
222 219 198 191 129 106 67
SfoI GGC'GCC
Cuts at: 2662 2763 2662
Size: 101 6947
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 2667 2837 3096 4375 1872
Size: 795 170 259 1279 4545
Fragments arranged by size:
4545 1279 795 259 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2732 3654 5152 6053 6315 820
Size: 197 926 789 922 1498 901 262 1553
Fragments arranged by size:
1553 1498 926 922 901 789 262 197
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SspI AAT'ATT
Cuts at: 5 52 2285 4972 5
Size: 47 2233 2687 2081
Fragments arranged by size:
2687 2233 2081 47
StuI AGG'CCT
Cuts at: 1517 2498 3035 1517
Size: 981 537 5530
Fragments arranged by size:
5530 981 537
StyI C'CwwG_G
Cuts at: 513 1065 2004 3217 3558 4487 513
Size: 552 939 1213 341 929 3074
Fragments arranged by size:
3074 1213 939 929 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2896 4060 4319 5075 5092 5245 5430
Size: 1255 1316 1164 259 756 17 153 185
Cuts at: 5430 5875 325
Size: 445 1498
Fragments arranged by size:
1498 1316 1255 1164 756 445 259 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3411 3720 5294
Size: 274 80 33 51 1443 1434 309 1574
Cuts at: 5294 7006 96
Size: 1712 138
Fragments arranged by size:
1712 1574 1443 1434 309 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7008 7040 7008
Size: 32 7016
Tth111I GACn'n_nGTC
Cuts at: 1316 4048 1316
Size: 2732 4316
XbaI T'CTAG_A
Cuts at: 2791 4384 2791
Size: 1593 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 2665 2835 3094 4373 1870
Size: 795 170 259 1279 4545
Fragments arranged by size:
4545 1279 795 259 170
XmnI GAAnn'nnTTC
Cuts at: 5177 5177
Size: 7048
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BsmBI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI
XcmI