Restriction map for
pFN21AB5791
(Circular) (Six-base) MAPSORT of: pFN21AB5791.seq Check: 9258 from: 1 to: 7195
pFN21AB5791 7195 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2558 3443 3803 2558
Size: 885 360 5950
Fragments arranged by size:
5950 885 360
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5005 278
Size: 53 83 186 936 3469 2468
Fragments arranged by size:
3469 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4538 1937
Size: 2601 4594
Acc65I G'GTAC_C
Cuts at: 2703 3411 4516 2703
Size: 708 1105 5382
Fragments arranged by size:
5382 1105 708
AclI AA'CG_TT
Cuts at: 1563 5322 5695 1563
Size: 3759 373 3063
Fragments arranged by size:
3759 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2819 2857 3276 4053 4159 5258 6642
Size: 433 754 38 419 777 106 1099 1384
Cuts at: 6642 6910 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1099 777 754 433 419 268
106 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6998
AflIII A'CryG_T
Cuts at: 1203 2499 2626 3608 4126 6094 7071 1203
Size: 1296 127 982 518 1968 977 1327
Fragments arranged by size:
1968 1327 1296 982 977 518 127
AgeI A'CCGG_T
Cuts at: 6026 6026
Size: 7195
AhdI GACnn_n'nnGTC
Cuts at: 5924 5924
Size: 7195
AleI CACnn'nnGTG
Cuts at: 1166 3700 1166
Size: 2534 4661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3557 1184
Size: 2373 4822
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2601 2633 2601
Size: 32 7163
AlwNI CAG_nnn'CTG
Cuts at: 2429 2983 4938 6510 2429
Size: 554 1955 1572 3114
Fragments arranged by size:
3114 1955 1572 554
ApaI G_GGCC'C
Cuts at: 2721 3837 2721
Size: 1116 6079
ApaLI G'TGCA_C
Cuts at: 5252 6408 5252
Size: 1156 6039
ApoI r'AATT_y
Cuts at: 1493 2061 3056 3499 3776 4504 4730 4882 6991
Size: 568 995 443 277 728 226 152 2109
Cuts at: 6991 1493
Size: 1697
Fragments arranged by size:
2109 1697 995 728 568 443 277 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5749 160
Size: 872 4717 1606
Fragments arranged by size:
4717 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7195
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 2929 3375 4486 4520 1870
Size: 73 585 401 446 1111 34 4545
Fragments arranged by size:
4545 1111 585 446 401 73 34
AvrII C'CTAG_G
Cuts at: 4447 4447
Size: 7195
BamHI G'GATC_C
Cuts at: 2283 4525 4892 2283
Size: 2242 367 4586
Fragments arranged by size:
4586 2242 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2703 3401 3411 4516 5971
Size: 325 217 627 916 698 10 1105 1455
Cuts at: 5971 618
Size: 1842
Fragments arranged by size:
1842 1455 1105 916 698 627 325 217
10
BanII G_rGCy'C
Cuts at: 1391 2619 2721 3837 4514 1391
Size: 1228 102 1116 677 4072
Fragments arranged by size:
4072 1228 1116 677 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5386 5420 1903
Size: 34 3449 34 3678
Fragments arranged by size:
3678 3449 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5087 6297 5087
Size: 1210 5985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7195
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3072 5806 136
Size: 107 122 71 631 605 1400 2734 1525
Fragments arranged by size:
2734 1525 1400 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7190 7190
Size: 7195
BlpI GC'TnA_GC
Cuts at: 3545 4612 3545
Size: 1067 6128
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2721 2792 3722 3837 5256 6412 1790
Size: 299 632 71 930 115 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 930 632 299 115 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2362 2779 2948
Size: 416 53 83 399 24 945 417 169
Cuts at: 2948 3320 3353 4926 5884 442
Size: 372 33 1573 958 1753
Fragments arranged by size:
1753 1573 958 945 417 416 399 372
169 83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2543
Size: 7195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3031 3191 3495 3848 3900 4077 5855 1239
Size: 1792 160 304 353 52 177 1778 2579
Fragments arranged by size:
2579 1792 1778 353 304 177 160 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 3232 5320 6185 6483 2633
Size: 599 2088 865 298 3345
Fragments arranged by size:
3345 2088 865 599 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 5858 882
Size: 617 1026 3333 2219
Fragments arranged by size:
3333 2219 1026 617
BsaAI yAC'GTr
Cuts at: 493 1204 2593 2629 4986 493
Size: 711 1389 36 2357 2702
Fragments arranged by size:
2702 2357 1389 711 36
BsaBI GATnn'nnATC
Cuts at: 2282 2408 7189 2282
Size: 126 4781 2288
Fragments arranged by size:
4781 2288 126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5002 5384 6019 275
Size: 53 83 186 936 3469 382 635 1451
Fragments arranged by size:
3469 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2905 5628 6026 6300 6447 6858 2905
Size: 2723 398 274 147 411 3242
Fragments arranged by size:
3242 2723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2471 2501 2537
Size: 30 165 30 846 30 192 30 36
Cuts at: 2537 2567 2601 2631 3453 3483 1178
Size: 30 34 30 822 30 4890
Fragments arranged by size:
4890 846 822 192 165 36 34 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3288 1186
Size: 2102 5093
BseYI C'CCAG_C
Cuts at: 2720 2947 6398 6894 2720
Size: 227 3451 496 3021
Fragments arranged by size:
3451 3021 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2677 2701 2990 3114 3517 4218 1163
Size: 573 941 24 289 124 403 701 4140
Fragments arranged by size:
4140 941 701 573 403 289 124 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3674 4645 5406 6434 1540
Size: 275 186 1673 971 761 1028 2301
Fragments arranged by size:
2301 1673 1028 971 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 2619 2634 5256 5341 6412 2451
Size: 168 15 2622 85 1071 3234
Fragments arranged by size:
3234 2622 1071 168 85 15
BsmI GAATG_Cn'
Cuts at: 2738 2846 3521 3854 4706 4799 2738
Size: 108 675 333 852 93 5134
Fragments arranged by size:
5134 852 675 333 108 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2451 2619 2634 2721 2792 3722
Size: 399 299 362 168 15 87 71 930
Cuts at: 3722 3837 4514 5256 5341 6412 1391
Size: 115 677 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 677 399 362 299
168 115 87 85 71 15
BspEI T'CCGG_A
Cuts at: 6858 6858
Size: 7195
BspHI T'CATG_A
Cuts at: 2634 5084 6814 2634
Size: 2450 1730 3015
Fragments arranged by size:
3015 2450 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 2834 3443 3803 3896 4292 4550 844
Size: 1714 276 609 360 93 396 258 3489
Fragments arranged by size:
3489 1714 609 396 360 276 258 93
BsrBI CCG'CTC
Cuts at: 1394 1823 3025 4337 5082 1394
Size: 429 1202 1312 745 3507
Fragments arranged by size:
3507 1312 1202 745 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2924 3445 3932 5690 5864 65
Size: 1183 1676 521 487 1758 174 1396
Fragments arranged by size:
1758 1676 1396 1183 521 487 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5839 6026 1700
Size: 110 4029 187 2869
Fragments arranged by size:
4029 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7195
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7195
BssSI C'ACGA_G
Cuts at: 3768 5255 6267 3768
Size: 1487 1012 4696
Fragments arranged by size:
4696 1487 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3198 794
Size: 2404 4791
BstBI TT'CG_AA
Cuts at: 5999 5999
Size: 7195
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7195
BstXI CCAn_nnnn'nTGG
Cuts at: 2714 2728 3844 4256 2714
Size: 14 1116 412 5653
Fragments arranged by size:
5653 1116 412 14
BstYI r'GATC_y
Cuts at: 1972 2283 3352 4525 4892 5277 5294 6746 6832
Size: 311 1069 1173 367 385 17 1452 86
Cuts at: 6832 6844 7190 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1173 1069 385 367 346 311
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3006 3184 513
Size: 552 939 1002 178 4524
Fragments arranged by size:
4524 1002 939 552 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4160 487
Size: 683 56 2934 3522
Fragments arranged by size:
3522 2934 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 2684 2689 2978 3027 3350 4711 5505 5525
Size: 235 5 289 49 323 1361 794 20
Cuts at: 5525 2449
Size: 4119
Fragments arranged by size:
4119 1361 794 323 289 235 49 20
5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7160
DraI TTT'AAA
Cuts at: 4499 4499
Size: 7195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6202 809
Size: 507 4886 1802
Fragments arranged by size:
4886 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2208 4258
Size: 54 1425 50 275 18 177 201 2050
Cuts at: 4258 4642 5531 7160 8
Size: 384 889 1629 43
Fragments arranged by size:
2050 1629 1425 889 384 275 201 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4642 1537
Size: 275 2830 4090
Fragments arranged by size:
4090 2830 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2877 5125 1353
Size: 1524 2248 3423
Fragments arranged by size:
3423 2248 1524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5782 6156 6302 387
Size: 1064 138 4193 374 146 1280
Fragments arranged by size:
4193 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2617 2617
Size: 7195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2819 2857 3031 3191 3276 3495
Size: 393 433 754 38 174 160 85 219
Cuts at: 3495 3848 3900 4053 4077 4159 5258 5855 6642
Size: 353 52 153 24 82 1099 597 787
Cuts at: 6642 6910 1239
Size: 268 1524
Fragments arranged by size:
1524 1099 787 754 597 433 393 353
268 219 174 160 153 85 82 52
38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 2811 3915 2243
Size: 568 1104 5523
Fragments arranged by size:
5523 1104 568
EcoO109I rG'GnC_Cy
Cuts at: 2248 3833 2248
Size: 1585 5610
EcoRI G'AATT_C
Cuts at: 3499 3776 4504 4882 3499
Size: 277 728 378 5812
Fragments arranged by size:
5812 728 378 277
FspI TGC'GCA
Cuts at: 5701 5701
Size: 7195
HaeII r_GCGC'y
Cuts at: 1678 6342 1678
Size: 4664 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4020 4052 5836 5868 5910
Size: 32 135 32 2448 32 1784 32 42
Cuts at: 5910 5942 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 1784 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4269 4539 4785 669
Size: 988 281 2331 270 246 3079
Fragments arranged by size:
3079 2331 988 281 270 246
HindIII A'AGCT_T
Cuts at: 748 3336 748
Size: 2588 4607
HpaI GTT'AAC
Cuts at: 4785 4785
Size: 7195
KpnI G_GTAC'C
Cuts at: 2707 3415 4520 2707
Size: 708 1105 5382
Fragments arranged by size:
5382 1105 708
MfeI C'AATT_G
Cuts at: 4794 4794
Size: 7195
MluI A'CGCG_T
Cuts at: 7071 7071
Size: 7195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2881 4182 6309 6493 1305
Size: 1576 1301 2127 184 2007
Fragments arranged by size:
2127 2007 1576 1301 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2210 4260 10
Size: 54 1425 343 177 201 2050 2945
Fragments arranged by size:
2945 2050 1425 343 201 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2158 2308 3700 5153 5512 5671 518
Size: 648 992 150 1392 1453 359 159 2042
Fragments arranged by size:
2042 1453 1392 992 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 3051 3105 3169 3249 4059 4611 5288 6436
Size: 1445 54 64 80 810 552 677 1148
Cuts at: 6436 6681 1606
Size: 245 2120
Fragments arranged by size:
2120 1445 1148 810 677 552 245 80
64 54
NcoI C'CATG_G
Cuts at: 513 1065 2004 3006 513
Size: 552 939 1002 4702
Fragments arranged by size:
4702 1002 939 552
NdeI CA'TA_TG
Cuts at: 387 4456 387
Size: 4069 3126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7195
NotI GC'GGCC_GC
Cuts at: 4642 4642
Size: 7195
NspI r_CATG'y
Cuts at: 1132 1229 2503 3100 3612 4101 4130 4248 4553
Size: 97 1274 597 512 489 29 118 305
Cuts at: 4553 6098 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1274 597 512 489 305 118
97 29
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7195
PciI A'CATG_T
Cuts at: 2499 3608 4126 6094 2499
Size: 1109 518 1968 3600
Fragments arranged by size:
3600 1968 1109 518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2653 4164 1108
Size: 942 603 1511 4139
Fragments arranged by size:
4139 1511 942 603
PfoI T'CCnGG_A
Cuts at: 3979 4308 3979
Size: 329 6866
PmeI GTTT'AAAC
Cuts at: 4499 4499
Size: 7195
PmlI CAC'GTG
Cuts at: 2629 2629
Size: 7195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2601 2633 5264 5296 6777 6809 2601
Size: 32 2631 32 1481 32 2987
Fragments arranged by size:
2987 2631 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2248
Size: 7195
PshAI GACnn'nnGTC
Cuts at: 1613 3124 4488 1613
Size: 1511 1364 4320
Fragments arranged by size:
4320 1511 1364
PsiI TTA'TAA
Cuts at: 3600 4765 3600
Size: 1165 6030
PspOMI G'GGCC_C
Cuts at: 2717 3833 2717
Size: 1116 6079
PstI C_TGCA'G
Cuts at: 830 2510 2645 2870 3428 4064 4547 830
Size: 1680 135 225 558 636 483 3478
Fragments arranged by size:
3478 1680 636 558 483 225 135
PvuI CG_AT'CG
Cuts at: 2001 3674 2001
Size: 1673 5522
PvuII CAG'CTG
Cuts at: 3051 4059 3051
Size: 1008 6187
SacI G_AGCT'C
Cuts at: 2619 2619
Size: 7195
SalI G'TCGA_C
Cuts at: 1936 4537 1936
Size: 2601 4594
SbfI CC_TGCA'GG
Cuts at: 4547 4547
Size: 7195
ScaI AGT'ACT
Cuts at: 5443 7155 5443
Size: 1712 5483
SfcI C'TryA_G
Cuts at: 826 1045 2260 2506 2641 2866 3076 3238 3424
Size: 219 1215 246 135 225 210 162 186
Cuts at: 3424 3473 3967 4060 4543 5678 6359 6550 826
Size: 49 494 93 483 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 681 494 483 246 225
219 210 191 186 162 135 93 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7195
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7195
SmaI CCC'GGG
Cuts at: 1872 3377 4522 1872
Size: 1505 1145 4545
Fragments arranged by size:
4545 1505 1145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 2612 3211 5299 6200 6462
Size: 197 926 585 84 599 2088 901 262
Cuts at: 6462 820
Size: 1553
Fragments arranged by size:
2088 1553 926 901 599 585 262 197
84
SnaBI TAC'GTA
Cuts at: 493 4986 493
Size: 4493 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7195
SphI G_CATG'C
Cuts at: 1132 4101 4248 4553 1132
Size: 2969 147 305 3774
Fragments arranged by size:
3774 2969 305 147
SspI AAT'ATT
Cuts at: 5 52 2167 5119 5
Size: 47 2115 2952 2081
Fragments arranged by size:
2952 2115 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7195
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 4447 4634 513
Size: 552 939 1002 1441 187 3074
Fragments arranged by size:
3074 1441 1002 939 552 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 4355 5222 5239 5392 5577 6022
Size: 1255 722 2053 867 17 153 185 445
Cuts at: 6022 325
Size: 1498
Fragments arranged by size:
2053 1498 1255 867 722 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 3963 5441
Size: 274 80 33 51 1443 1688 298 1478
Cuts at: 5441 7153 96
Size: 1712 138
Fragments arranged by size:
1712 1688 1478 1443 298 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2895 2927 3453 3485 7155 7187 2895
Size: 32 526 32 3670 32 2903
Fragments arranged by size:
3670 2903 526 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7195
XbaI T'CTAG_A
Cuts at: 4207 4531 4207
Size: 324 6871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4223 4223
Size: 7195
XhoI C'TCGA_G
Cuts at: 1943 2528 1943
Size: 585 6610
XmaI C'CCGG_G
Cuts at: 1870 3375 4520 1870
Size: 1505 1145 4545
Fragments arranged by size:
4545 1505 1145
XmnI GAAnn'nnTTC
Cuts at: 5324 5324
Size: 7195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5003 276
Size: 53 83 186 936 3469 2468
Fragments arranged by size:
3469 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI