Restriction map for
pFN21AB5790
(Circular) (Six-base) MAPSORT of: pFN21AB5790.seq Check: 6178 from: 1 to: 7036
pFN21AB5790 7036 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4085 4085
Size: 7036
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3922 4379 1937
Size: 1985 457 4594
Fragments arranged by size:
4594 1985 457
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 5163 5536 1563
Size: 3600 373 3063
Fragments arranged by size:
3600 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 2853 3446 3723 3987 5099 6483 6751
Size: 840 381 593 277 264 1112 1384 268
Cuts at: 6751 1632
Size: 1917
Fragments arranged by size:
1917 1384 1112 840 593 381 277 268
264
AfeI AGC'GCT
Cuts at: 4196 4196
Size: 7036
AflII C'TTAA_G
Cuts at: 820 1017 3779 820
Size: 197 2762 4077
Fragments arranged by size:
4077 2762 197
AflIII A'CryG_T
Cuts at: 1203 3150 5935 6912 1203
Size: 1947 2785 977 1327
Fragments arranged by size:
2785 1947 1327 977
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7036
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 2236
Size: 32 7004
AlwNI CAG_nnn'CTG
Cuts at: 2810 4779 6351 2810
Size: 1969 1572 3495
Fragments arranged by size:
3495 1969 1572
ApaI G_GGCC'C
Cuts at: 2110 2110
Size: 7036
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 2341 2425 2546 2659 2687 3161 3899 3960
Size: 848 84 121 113 28 474 738 61
Cuts at: 3960 4345 4571 4723 6832 1493
Size: 385 226 152 2109 1697
Fragments arranged by size:
2109 1697 848 738 474 385 226 152
121 113 84 61 28
AseI AT'TA_AT
Cuts at: 160 1032 5590 160
Size: 872 4558 1606
Fragments arranged by size:
4558 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 4361 1870
Size: 73 2418 4545
Fragments arranged by size:
4545 2418 73
AvrII C'CTAG_G
Cuts at: 2387 2387
Size: 7036
BamHI G'GATC_C
Cuts at: 2043 4366 4733 2043
Size: 2323 367 4346
Fragments arranged by size:
4346 2323 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 3058 3420 4357 5812
Size: 325 217 627 312 959 362 937 1455
Cuts at: 5812 618
Size: 1842
Fragments arranged by size:
1842 1455 959 937 627 362 325 312
217
BanII G_rGCy'C
Cuts at: 1391 2110 4355 1391
Size: 719 2245 4072
Fragments arranged by size:
4072 2245 719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3729 928
Size: 971 1830 4235
Fragments arranged by size:
4235 1830 971
BbvCI CC'TCA_GC
Cuts at: 3238 3661 3238
Size: 423 6613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5227 5261 1903
Size: 34 50 34 3206 34 3678
Fragments arranged by size:
3678 3206 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2271 2734 4928 6138 2271
Size: 463 2194 1210 3169
Fragments arranged by size:
3169 2194 1210 463
BclI T'GATC_A
Cuts at: 1548 4189 1548
Size: 2641 4395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5647 136
Size: 107 122 71 631 605 3975 1525
Fragments arranged by size:
3975 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2606 7031 2606
Size: 4425 2611
BlpI GC'TnA_GC
Cuts at: 2529 2853 4453 2529
Size: 324 1600 5112
Fragments arranged by size:
5112 1600 324
Bme1580I G_kGCm'C
Cuts at: 1790 2110 3063 3423 5097 6253 1790
Size: 320 953 360 1674 1156 2573
Fragments arranged by size:
2573 1674 1156 953 360 320
BmgBI CAC'GTC
Cuts at: 1318 4141 1318
Size: 2823 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3699 4767 5725
Size: 416 53 83 399 24 2282 1068 958
Cuts at: 5725 442
Size: 1753
Fragments arranged by size:
2282 1753 1068 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3669 1056
Size: 2613 4423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2737 2853 3431 3544 5696 1239
Size: 917 581 116 578 113 2152 2579
Fragments arranged by size:
2579 2152 917 581 578 116 113
Bpu10I CC'TnA_GC
Cuts at: 3238 3661 3238
Size: 423 6613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5161 6026 6324 5161
Size: 865 298 5873
Fragments arranged by size:
5873 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4171 5699 882
Size: 617 2672 1528 2219
Fragments arranged by size:
2672 2219 1528 617
BsaAI yAC'GTr
Cuts at: 493 1204 4827 493
Size: 711 3623 2702
Fragments arranged by size:
3623 2702 711
BsaBI GATnn'nnATC
Cuts at: 4188 7030 4188
Size: 2842 4194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4843 5225 5860 275
Size: 53 83 186 936 3310 382 635 1451
Fragments arranged by size:
3310 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2086 5469 5867 6141 6288 6699 2086
Size: 3383 398 274 147 411 2423
Fragments arranged by size:
3383 2423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3030 3060 1178
Size: 30 165 30 1627 30 5154
Fragments arranged by size:
5154 1627 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3300 1186
Size: 2114 4922
BseYI C'CCAG_C
Cuts at: 2499 6239 6735 2499
Size: 3740 496 2800
Fragments arranged by size:
3740 2800 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4186 1163
Size: 573 2450 4013
Fragments arranged by size:
4013 2450 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 4486 5247 6275 1540
Size: 275 186 130 2355 761 1028 2301
Fragments arranged by size:
2355 2301 1028 761 275 186 130
BsiHKAI G_wGCw'C
Cuts at: 2208 2530 3032 5097 5182 6253 2208
Size: 322 502 2065 85 1071 2991
Fragments arranged by size:
2991 2065 1071 502 322 85
BsmI GAATG_Cn'
Cuts at: 3021 4547 4640 3021
Size: 1526 93 5417
Fragments arranged by size:
5417 1526 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2208 2530 3032 3063 3423 4355
Size: 399 320 98 322 502 31 360 932
Cuts at: 4355 5097 5182 6253 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 932 742 502 399 360 322
320 98 85 31
BspEI T'CCGG_A
Cuts at: 6699 6699
Size: 7036
BspHI T'CATG_A
Cuts at: 4925 6655 4925
Size: 1730 5306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4085 4391 844
Size: 3241 306 3489
Fragments arranged by size:
3489 3241 306
BsrBI CCG'CTC
Cuts at: 1394 1823 2049 4923 1394
Size: 429 226 2874 3507
Fragments arranged by size:
3507 2874 429 226
BsrDI GCAATG_nn'
Cuts at: 65 1248 3077 3249 3395 5531 5705 65
Size: 1183 1829 172 146 2136 174 1396
Fragments arranged by size:
2136 1829 1396 1183 174 172 146
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 5680 5867 1700
Size: 110 221 3649 187 2869
Fragments arranged by size:
3649 2869 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7036
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7036
BssSI C'ACGA_G
Cuts at: 5096 6108 5096
Size: 1012 6024
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3058 4172 794
Size: 2264 1114 3658
Fragments arranged by size:
3658 2264 1114
BstBI TT'CG_AA
Cuts at: 2338 5840 2338
Size: 3502 3534
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7036
BstYI r'GATC_y
Cuts at: 1972 2043 2606 4107 4366 4733 5118 5135 6587
Size: 71 563 1501 259 367 385 17 1452
Cuts at: 6587 6673 6685 7031 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1501 1452 563 385 367 346 259
86 71 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 513
Size: 552 1014 5470
Fragments arranged by size:
5470 1014 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6297
Fragments arranged by size:
6297 683 56
BtsI GCAGTG_nn'
Cuts at: 3670 3733 4089 4552 5346 5366 3670
Size: 63 356 463 794 20 5340
Fragments arranged by size:
5340 794 463 356 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7036
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 4340 4340
Size: 7036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2991 6043 809
Size: 507 1675 3052 1802
Fragments arranged by size:
3052 1802 1675 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2971 4483 5372
Size: 54 1425 50 275 18 1141 1512 889
Cuts at: 5372 7001 8
Size: 1629 43
Fragments arranged by size:
1629 1512 1425 1141 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4483 1537
Size: 275 2671 4090
Fragments arranged by size:
4090 2671 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4966 1353
Size: 3613 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 5623 5997 6143 387
Size: 1064 138 476 3558 374 146 1280
Fragments arranged by size:
3558 1280 1064 476 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2472 2737 2853 3431 3446 3544
Size: 393 524 316 265 116 578 15 98
Cuts at: 3544 3723 3987 5099 5696 6483 6751 1239
Size: 179 264 1112 597 787 268 1524
Fragments arranged by size:
1524 1112 787 597 578 524 393 316
268 265 264 179 116 98 15
EcoRI G'AATT_C
Cuts at: 2425 3161 4345 4723 2425
Size: 736 1184 378 4738
Fragments arranged by size:
4738 1184 736 378
FspI TGC'GCA
Cuts at: 5542 5542
Size: 7036
HaeII r_GCGC'y
Cuts at: 1678 4198 6183 1678
Size: 2520 1985 2531
Fragments arranged by size:
2531 2520 1985
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3217 3249 5677 5709 5751
Size: 32 135 32 1645 32 2428 32 42
Cuts at: 5751 5783 1373
Size: 32 2626
Fragments arranged by size:
2626 2428 1645 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4380 4626 669
Size: 988 281 2442 246 3079
Fragments arranged by size:
3079 2442 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2333 2484 3405 748
Size: 1585 151 921 4379
Fragments arranged by size:
4379 1585 921 151
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 4635 4635
Size: 7036
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6150 6334 1305
Size: 4845 184 2007
Fragments arranged by size:
4845 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2973 10
Size: 54 1425 343 1141 4073
Fragments arranged by size:
4073 1425 1141 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 3103 4994 5353 5512 518
Size: 648 1189 748 1891 359 159 2042
Fragments arranged by size:
2042 1891 1189 748 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4003 4452 5129 6277 6522 1606
Size: 2397 449 677 1148 245 2120
Fragments arranged by size:
2397 2120 1148 677 449 245
NcoI C'CATG_G
Cuts at: 513 1065 2079 513
Size: 552 1014 5470
Fragments arranged by size:
5470 1014 552
NdeI CA'TA_TG
Cuts at: 387 2542 387
Size: 2155 4881
NheI G'CTAG_C
Cuts at: 1052 3665 1052
Size: 2613 4423
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 3154 3524 3921 4394 5939 1132
Size: 97 1925 370 397 473 1545 2229
Fragments arranged by size:
2229 1925 1545 473 397 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7036
PciI A'CATG_T
Cuts at: 3150 5935 3150
Size: 2785 4251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7036
PfoI T'CCnGG_A
Cuts at: 2051 2051
Size: 7036
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5105 5137 6618 6650 5105
Size: 32 1481 32 5491
Fragments arranged by size:
5491 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7036
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PspOMI G'GGCC_C
Cuts at: 2106 2106
Size: 7036
PstI C_TGCA'G
Cuts at: 830 2572 4096 4388 830
Size: 1742 1524 292 3478
Fragments arranged by size:
3478 1742 1524 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7036
PvuII CAG'CTG
Cuts at: 4003 4003
Size: 7036
SalI G'TCGA_C
Cuts at: 1936 4378 1936
Size: 2442 4594
SbfI CC_TGCA'GG
Cuts at: 4388 4388
Size: 7036
ScaI AGT'ACT
Cuts at: 5284 6996 5284
Size: 1712 5324
SexAI A'CCwGG_T
Cuts at: 3178 3178
Size: 7036
SfcI C'TryA_G
Cuts at: 826 1045 2215 2568 2731 4092 4384 5519 6200
Size: 219 1170 353 163 1361 292 1135 681
Cuts at: 6200 6391 826
Size: 191 1471
Fragments arranged by size:
1471 1361 1170 1135 681 353 292 219
191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7036
SmaI CCC'GGG
Cuts at: 1872 4363 1872
Size: 2491 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3779 5140 6041 6303 820
Size: 197 926 1836 1361 901 262 1553
Fragments arranged by size:
1836 1553 1361 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4827 493
Size: 4334 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 4394 1132
Size: 3262 3774
SspI AAT'ATT
Cuts at: 5 52 4960 5
Size: 47 4908 2081
Fragments arranged by size:
4908 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7036
StyI C'CwwG_G
Cuts at: 513 1065 2079 2387 4475 513
Size: 552 1014 308 2088 3074
Fragments arranged by size:
3074 2088 1014 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4257 5063 5080 5233 5418 5863 325
Size: 1255 2677 806 17 153 185 445 1498
Fragments arranged by size:
2677 1498 1255 806 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2162 3715 3809
Size: 274 80 33 51 1443 185 1553 94
Cuts at: 3809 5282 6994 96
Size: 1473 1712 138
Fragments arranged by size:
1712 1553 1473 1443 274 185 138 94
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2241 2273 6996 7028 2241
Size: 32 4723 32 2249
Fragments arranged by size:
4723 2249 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 4372 4372
Size: 7036
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7036
XmaI C'CCGG_G
Cuts at: 1870 4361 1870
Size: 2491 4545
XmnI GAAnn'nnTTC
Cuts at: 5165 5165
Size: 7036
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI