Restriction map for
pFN21AB5781
(Circular) (Six-base) MAPSORT of: pFN21AB5781.seq Check: 329 from: 1 to: 7183
pFN21AB5781 7183 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3595 3595
Size: 7183
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4993 278
Size: 53 83 186 936 3457 2468
Fragments arranged by size:
3457 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4526 1937
Size: 2589 4594
Acc65I G'GTAC_C
Cuts at: 4504 4504
Size: 7183
AclI AA'CG_TT
Cuts at: 1563 5310 5683 1563
Size: 3747 373 3063
Fragments arranged by size:
3747 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 3699 5246 6630 6898 1632
Size: 812 1255 1547 1384 268 1917
Fragments arranged by size:
1917 1547 1384 1255 812 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6986
AflIII A'CryG_T
Cuts at: 1203 2034 6082 7059 1203
Size: 831 4048 977 1327
Fragments arranged by size:
4048 1327 977 831
AgeI A'CCGG_T
Cuts at: 6014 6014
Size: 7183
AhdI GACnn_n'nnGTC
Cuts at: 5912 5912
Size: 7183
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3312 1184
Size: 2128 5055
AlwNI CAG_nnn'CTG
Cuts at: 2375 2414 4188 4926 6498 2375
Size: 39 1774 738 1572 3060
Fragments arranged by size:
3060 1774 1572 738 39
ApaI G_GGCC'C
Cuts at: 2088 2088
Size: 7183
ApaLI G'TGCA_C
Cuts at: 4004 4476 5240 6396 4004
Size: 472 764 1156 4791
Fragments arranged by size:
4791 1156 764 472
ApoI r'AATT_y
Cuts at: 1493 2562 3206 3409 3421 3829 4492 4718 4870
Size: 1069 644 203 12 408 663 226 152
Cuts at: 4870 6979 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1069 663 644 408 226 203
152 12
AseI AT'TA_AT
Cuts at: 160 1032 3345 5737 160
Size: 872 2313 2392 1606
Fragments arranged by size:
2392 2313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 4508 1870
Size: 73 238 2327 4545
Fragments arranged by size:
4545 2327 238 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2511 2544 3302 3335 2511
Size: 33 758 33 6359
Fragments arranged by size:
6359 758 33 33
BamHI G'GATC_C
Cuts at: 3193 4513 4880 3193
Size: 1320 367 5496
Fragments arranged by size:
5496 1320 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 4504 5959 618
Size: 325 217 627 224 2493 1455 1842
Fragments arranged by size:
2493 1842 1455 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2081 2088 2965 4502 1391
Size: 690 7 877 1537 4072
Fragments arranged by size:
4072 1537 877 690 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6212
BbvCI CC'TCA_GC
Cuts at: 3754 3754
Size: 7183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2172 2206 5374 5408 1903
Size: 34 235 34 3168 34 3678
Fragments arranged by size:
3678 3168 235 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3931 5075 6285 3931
Size: 1144 1210 4829
Fragments arranged by size:
4829 1210 1144
BclI T'GATC_A
Cuts at: 1548 2587 3069 3229 4276 1548
Size: 1039 482 160 1047 4455
Fragments arranged by size:
4455 1047 1039 482 160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5794 136
Size: 107 122 71 631 605 337 3785 1525
Fragments arranged by size:
3785 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2665 7178 2665
Size: 4513 2670
BlpI GC'TnA_GC
Cuts at: 4600 4600
Size: 7183
Bme1580I G_kGCm'C
Cuts at: 1790 2088 3702 4008 4480 5244 6400 1790
Size: 298 1614 306 472 764 1156 2573
Fragments arranged by size:
2573 1614 1156 764 472 306 298
BmgBI CAC'GTC
Cuts at: 1318 2112 4159 1318
Size: 794 2047 4342
Fragments arranged by size:
4342 2047 794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2281 2866 4071
Size: 416 53 83 399 24 864 585 1205
Cuts at: 4071 4187 4914 5872 442
Size: 116 727 958 1753
Fragments arranged by size:
1753 1205 958 864 727 585 416 399
116 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2843 1056
Size: 1787 5396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4120 4120
Size: 7183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2095 2265 2849 2949 3126 3387 5843
Size: 803 53 170 584 100 177 261 2456
Cuts at: 5843 1239
Size: 2579
Fragments arranged by size:
2579 2456 803 584 261 177 170 100
53
Bpu10I CC'TnA_GC
Cuts at: 3754 3754
Size: 7183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5308 6173 6471 5308
Size: 865 298 6020
Fragments arranged by size:
6020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5846 882
Size: 617 4347 2219
Fragments arranged by size:
4347 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 4219 4974 493
Size: 711 831 2184 755 2702
Fragments arranged by size:
2702 2184 831 755 711
BsaBI GATnn'nnATC
Cuts at: 2926 3477 7177 2926
Size: 551 3700 2932
Fragments arranged by size:
3700 2932 551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4990 5372 6007 275
Size: 53 83 186 936 3457 382 635 1451
Fragments arranged by size:
3457 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5616 6014 6288 6435 6846 5616
Size: 398 274 147 411 5953
Fragments arranged by size:
5953 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3483 3513 3622 3652 1178
Size: 30 165 30 2080 30 109 30 4709
Fragments arranged by size:
4709 2080 165 109 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2374 2652 3584 4135 1186
Size: 1188 278 932 551 4234
Fragments arranged by size:
4234 1188 932 551 278
BseYI C'CCAG_C
Cuts at: 6386 6882 6386
Size: 496 6687
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3384 3845 1163
Size: 573 1648 461 4501
Fragments arranged by size:
4501 1648 573 461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4633 5394 6422 1540
Size: 275 186 2632 761 1028 2301
Fragments arranged by size:
2632 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 2130 3462 4008 4128 4200 4480 5244 5329
Size: 49 1332 546 120 72 280 764 85
Cuts at: 5329 6400 2081
Size: 1071 2864
Fragments arranged by size:
2864 1332 1071 764 546 280 120 85
72 49
BsmI GAATG_Cn'
Cuts at: 4694 4787 4694
Size: 93 7090
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2088 2130 2965 3462 3702 4008
Size: 399 291 7 42 835 497 240 306
Cuts at: 4008 4128 4200 4480 4502 5244 5329 6400 1391
Size: 120 72 280 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 835 742 497 399 306 291
280 240 120 85 72 42 22 7
BspEI T'CCGG_A
Cuts at: 6846 6846
Size: 7183
BspHI T'CATG_A
Cuts at: 2848 5072 6802 2848
Size: 2224 1730 3229
Fragments arranged by size:
3229 2224 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3595 4538 844
Size: 2751 943 3489
Fragments arranged by size:
3489 2751 943
BsrBI CCG'CTC
Cuts at: 1394 1823 5070 1394
Size: 429 3247 3507
Fragments arranged by size:
3507 3247 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 3072 3345 5678 5852 65
Size: 1183 1306 518 273 2333 174 1396
Fragments arranged by size:
2333 1396 1306 1183 518 273 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2843 5827 6014 1700
Size: 110 1033 2984 187 2869
Fragments arranged by size:
2984 2869 1033 187 110
BsrGI T'GTAC_A
Cuts at: 96 2384 96
Size: 2288 4895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7183
BssSI C'ACGA_G
Cuts at: 5243 6255 5243
Size: 1012 6171
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2115 2414 4406 794
Size: 1321 299 1992 3571
Fragments arranged by size:
3571 1992 1321 299
BstBI TT'CG_AA
Cuts at: 5987 5987
Size: 7183
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7183
BstXI CCAn_nnnn'nTGG
Cuts at: 3135 3135
Size: 7183
BstYI r'GATC_y
Cuts at: 1972 2249 2371 2665 3193 4513 4880 5265 5282
Size: 277 122 294 528 1320 367 385 17
Cuts at: 5282 6734 6820 6832 7178 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1320 528 385 367 346 294
277 122 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2937 3088 3254 513
Size: 552 939 117 816 151 166 4442
Fragments arranged by size:
4442 939 816 552 166 151 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6444
Fragments arranged by size:
6444 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 4381 4699 5493 5513 2310
Size: 2071 318 794 20 3980
Fragments arranged by size:
3980 2071 794 318 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7148
DraI TTT'AAA
Cuts at: 2298 2469 4487 2298
Size: 171 2018 4994
Fragments arranged by size:
4994 2018 171
DraIII CAC_nnn'GTG
Cuts at: 4450 4450
Size: 7183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6190 809
Size: 507 4874 1802
Fragments arranged by size:
4874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4630 5519 7148
Size: 54 1425 50 275 18 2800 889 1629
Cuts at: 7148 8
Size: 43
Fragments arranged by size:
2800 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4630 1537
Size: 275 2818 4090
Fragments arranged by size:
4090 2818 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2967 3254 3561 5113 1353
Size: 1614 287 307 1552 3423
Fragments arranged by size:
3423 1614 1552 307 287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5770 6144 6290 387
Size: 1064 138 4181 374 146 1280
Fragments arranged by size:
4181 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2079 2079
Size: 7183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2095 2265 2444 2849 2949 3126
Size: 393 410 53 170 179 405 100 177
Cuts at: 3126 3387 3699 5246 5843 6630 6898 1239
Size: 261 312 1547 597 787 268 1524
Fragments arranged by size:
1547 1524 787 597 410 405 393 312
268 261 179 177 170 100 53
EcoO109I rG'GnC_Cy
Cuts at: 2327 2327
Size: 7183
EcoRI G'AATT_C
Cuts at: 2562 4492 4870 2562
Size: 1930 378 4875
Fragments arranged by size:
4875 1930 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4072 4270 4072
Size: 198 6985
FspI TGC'GCA
Cuts at: 5689 5689
Size: 7183
HaeII r_GCGC'y
Cuts at: 1678 6330 1678
Size: 4652 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 2960 2992 3061
Size: 32 135 32 784 32 572 32 69
Cuts at: 3061 3093 3483 3515 3929 3961 5824 5856 5898
Size: 32 390 32 414 32 1863 32 42
Cuts at: 5898 5930 1373
Size: 32 2626
Fragments arranged by size:
2626 1863 784 572 414 390 135 69
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 3124 3579 4527 4773 669
Size: 988 281 792 394 455 948 246 3079
Fragments arranged by size:
3079 988 948 792 455 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7183
HpaI GTT'AAC
Cuts at: 3124 3579 4773 3124
Size: 455 1194 5534
Fragments arranged by size:
5534 1194 455
KpnI G_GTAC'C
Cuts at: 4508 4508
Size: 7183
MfeI C'AATT_G
Cuts at: 2188 4782 2188
Size: 2594 4589
MluI A'CGCG_T
Cuts at: 7059 7059
Size: 7183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6297 6481 1305
Size: 4992 184 2007
Fragments arranged by size:
4992 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5361
Fragments arranged by size:
5361 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3168 3318 3705 3860 5141 5500 5659
Size: 648 2002 150 387 155 1281 359 159
Cuts at: 5659 518
Size: 2042
Fragments arranged by size:
2042 2002 1281 648 387 359 159 155
150
MspA1I CmG'CkG
Cuts at: 1606 2405 4178 4599 5276 6424 6669 1606
Size: 799 1773 421 677 1148 245 2120
Fragments arranged by size:
2120 1773 1148 799 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2937 3254 513
Size: 552 939 933 317 4442
Fragments arranged by size:
4442 939 933 552 317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7183
NheI G'CTAG_C
Cuts at: 1052 2839 1052
Size: 1787 5396
NotI GC'GGCC_GC
Cuts at: 4630 4630
Size: 7183
NspI r_CATG'y
Cuts at: 1132 1229 2348 2544 3785 4541 6086 1132
Size: 97 1119 196 1241 756 1545 2229
Fragments arranged by size:
2229 1545 1241 1119 756 196 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7183
PciI A'CATG_T
Cuts at: 6082 6082
Size: 7183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7183
PfoI T'CCnGG_A
Cuts at: 4152 4152
Size: 7183
PmeI GTTT'AAAC
Cuts at: 4487 4487
Size: 7183
PmlI CAC'GTG
Cuts at: 2035 2035
Size: 7183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 5252 5284 6765 6797 2063
Size: 32 3157 32 1481 32 2449
Fragments arranged by size:
3157 2449 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 2327
Size: 7183
PshAI GACnn'nnGTC
Cuts at: 1613 2428 4178 1613
Size: 815 1750 4618
Fragments arranged by size:
4618 1750 815
PsiI TTA'TAA
Cuts at: 4753 4753
Size: 7183
PspOMI G'GGCC_C
Cuts at: 2084 2084
Size: 7183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2853 2885 2853
Size: 32 7151
PstI C_TGCA'G
Cuts at: 830 2404 2410 3663 4535 830
Size: 1574 6 1253 872 3478
Fragments arranged by size:
3478 1574 1253 872 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7183
PvuII CAG'CTG
Cuts at: 2405 4178 2405
Size: 1773 5410
SacI G_AGCT'C
Cuts at: 2081 2081
Size: 7183
SalI G'TCGA_C
Cuts at: 1936 4525 1936
Size: 2589 4594
SbfI CC_TGCA'GG
Cuts at: 4535 4535
Size: 7183
ScaI AGT'ACT
Cuts at: 3586 5431 7143 3586
Size: 1845 1712 3626
Fragments arranged by size:
3626 1845 1712
SexAI A'CCwGG_T
Cuts at: 3914 3914
Size: 7183
SfcI C'TryA_G
Cuts at: 826 1045 2400 2406 2906 3659 4531 5666 6347
Size: 219 1355 6 500 753 872 1135 681
Cuts at: 6347 6538 826
Size: 191 1471
Fragments arranged by size:
1471 1355 1135 872 753 681 500 219
191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7183
SmaI CCC'GGG
Cuts at: 1872 4510 1872
Size: 2638 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5287 6188 6450 820
Size: 197 926 3344 901 262 1553
Fragments arranged by size:
3344 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4974 493
Size: 4481 2702
SpeI A'CTAG_T
Cuts at: 152 3581 152
Size: 3429 3754
SphI G_CATG'C
Cuts at: 1132 2348 3785 4541 1132
Size: 1216 1437 756 3774
Fragments arranged by size:
3774 1437 1216 756
SspI AAT'ATT
Cuts at: 5 52 2535 3714 5107 5
Size: 47 2483 1179 1393 2081
Fragments arranged by size:
2483 2081 1393 1179 47
StuI AGG'CCT
Cuts at: 1517 3304 1517
Size: 1787 5396
StyI C'CwwG_G
Cuts at: 513 1065 2004 2937 3254 4622 513
Size: 552 939 933 317 1368 3074
Fragments arranged by size:
3074 1368 939 933 552 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4151 5210 5227 5380 5565 6010 325
Size: 1255 2571 1059 17 153 185 445 1498
Fragments arranged by size:
2571 1498 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2384 3584 5429
Size: 274 80 33 51 1443 407 1200 1845
Cuts at: 5429 7141 96
Size: 1712 138
Fragments arranged by size:
1845 1712 1443 1200 407 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4294 4326 7143 7175 4294
Size: 32 2817 32 4302
Fragments arranged by size:
4302 2817 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3185 1316
Size: 1869 5314
XbaI T'CTAG_A
Cuts at: 4519 4519
Size: 7183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2095 2095
Size: 7183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7183
XmaI C'CCGG_G
Cuts at: 1870 4508 1870
Size: 2638 4545
XmnI GAAnn'nnTTC
Cuts at: 2997 3381 5312 2997
Size: 384 1931 4868
Fragments arranged by size:
4868 1931 384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4991 276
Size: 53 83 186 936 3457 2468
Fragments arranged by size:
3457 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI