Restriction map for
pFN21AB5778
(Circular) (Six-base) MAPSORT of: pFN21AB5778.seq Check: 8906 from: 1 to: 7003
pFN21AB5778 7003 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 4346 1937
Size: 2271 138 4594
Fragments arranged by size:
4594 2271 138
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 4009 4134 5130 5503 1563
Size: 2446 125 996 373 3063
Fragments arranged by size:
3063 2446 996 373 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2611 3066 3664 5066 6450 6718 1632
Size: 557 422 455 598 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 598 557 455 422 268
AflII C'TTAA_G
Cuts at: 820 1017 2182 820
Size: 197 1165 5641
Fragments arranged by size:
5641 1165 197
AflIII A'CryG_T
Cuts at: 1203 5902 6879 1203
Size: 4699 977 1327
Fragments arranged by size:
4699 1327 977
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7003
AlwNI CAG_nnn'CTG
Cuts at: 2786 3674 4746 6318 2786
Size: 888 1072 1572 3471
Fragments arranged by size:
3471 1572 1072 888
ApaLI G'TGCA_C
Cuts at: 5060 6216 5060
Size: 1156 5847
ApoI r'AATT_y
Cuts at: 1493 2539 2934 3572 4057 4084 4312 4538 4690
Size: 1046 395 638 485 27 228 226 152
Cuts at: 4690 6799 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1046 638 485 395 228 226
152 27
AseI AT'TA_AT
Cuts at: 160 1032 5557 160
Size: 872 4525 1606
Fragments arranged by size:
4525 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 4328 1870
Size: 73 2385 4545
Fragments arranged by size:
4545 2385 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2729 2762 2729
Size: 33 6970
BamHI G'GATC_C
Cuts at: 4333 4700 4333
Size: 367 6636
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3203 3964 4324 5779 618
Size: 325 217 627 1416 761 360 1455 1842
Fragments arranged by size:
1842 1455 1416 761 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 4322 1391
Size: 2931 4072
BbeI G_GCGC'C
Cuts at: 3968 3968
Size: 7003
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2617 3495 3670 3799 4041 4224
Size: 971 116 602 878 175 129 242 183
Cuts at: 4224 928
Size: 3707
Fragments arranged by size:
3707 971 878 602 242 183 175 129
116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5194 5228 1903
Size: 34 3257 34 3678
Fragments arranged by size:
3678 3257 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3982 4895 6105 3982
Size: 913 1210 4880
Fragments arranged by size:
4880 1210 913
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7003
BfrBI ATG'CAT
Cuts at: 3778 3778
Size: 7003
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5614 136
Size: 107 122 71 631 605 3942 1525
Fragments arranged by size:
3942 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4158 6998 4158
Size: 2840 4163
BlpI GC'TnA_GC
Cuts at: 4420 4420
Size: 7003
Bme1580I G_kGCm'C
Cuts at: 1790 2076 5064 6220 1790
Size: 286 2988 1156 2573
Fragments arranged by size:
2988 2573 1156 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7003
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2614 3700 3806
Size: 416 53 83 399 24 1197 1086 106
Cuts at: 3806 4734 5692 442
Size: 928 958 1753
Fragments arranged by size:
1753 1197 1086 958 928 416 399 106
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3735 4236 1056
Size: 2679 501 3823
Fragments arranged by size:
3823 2679 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4014 5663 1239
Size: 2775 1649 2579
Fragments arranged by size:
2775 2579 1649
Bpu10I CC'TnA_GC
Cuts at: 2951 2951
Size: 7003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5128 5993 6291 5128
Size: 865 298 5840
Fragments arranged by size:
5840 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 4025 5666 882
Size: 617 1159 1367 1641 2219
Fragments arranged by size:
2219 1641 1367 1159 617
BsaAI yAC'GTr
Cuts at: 493 1204 2365 3907 4794 493
Size: 711 1161 1542 887 2702
Fragments arranged by size:
2702 1542 1161 887 711
BsaBI GATnn'nnATC
Cuts at: 6997 6997
Size: 7003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3965 4810 5192 5827
Size: 53 83 186 936 2432 845 382 635
Cuts at: 5827 275
Size: 1451
Fragments arranged by size:
2432 1451 936 845 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3355 3422 5436 5834 6108 6255 6666 3355
Size: 67 2014 398 274 147 411 3692
Fragments arranged by size:
3692 2014 411 398 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3891 3921 1178
Size: 30 165 30 2488 30 4260
Fragments arranged by size:
4260 2488 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3588 1186
Size: 2402 4601
BseYI C'CCAG_C
Cuts at: 2038 3060 6206 6702 2038
Size: 1022 3146 496 2339
Fragments arranged by size:
3146 2339 1022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3169 1163
Size: 573 1433 4997
Fragments arranged by size:
4997 1433 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4453 5214 6242 1540
Size: 275 186 2452 761 1028 2301
Fragments arranged by size:
2452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5064 5149 6220 5064
Size: 85 1071 5847
Fragments arranged by size:
5847 1071 85
BsmI GAATG_Cn'
Cuts at: 2380 3282 4514 4607 2380
Size: 902 1232 93 4776
Fragments arranged by size:
4776 1232 902 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 4322 5064 5149 6220 1391
Size: 399 286 2246 742 85 1071 2174
Fragments arranged by size:
2246 2174 1071 742 399 286 85
BspEI T'CCGG_A
Cuts at: 3355 6666 3355
Size: 3311 3692
BspHI T'CATG_A
Cuts at: 2200 4068 4892 6622 2200
Size: 1868 824 1730 2581
Fragments arranged by size:
2581 1868 1730 824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4358 844
Size: 3514 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4890 1394
Size: 429 3067 3507
Fragments arranged by size:
3507 3067 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2378 3139 4206 5498 5672 65
Size: 1183 1130 761 1067 1292 174 1396
Fragments arranged by size:
1396 1292 1183 1130 1067 761 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5647 5834 1700
Size: 110 3837 187 2869
Fragments arranged by size:
3837 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3999 96
Size: 3903 3100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 5063 6075 5063
Size: 1012 5991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7003
BstBI TT'CG_AA
Cuts at: 5807 5807
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1184 2475 3755 1184
Size: 1291 1280 4432
Fragments arranged by size:
4432 1291 1280
BstXI CCAn_nnnn'nTGG
Cuts at: 3544 4171 3544
Size: 627 6376
BstYI r'GATC_y
Cuts at: 1972 4158 4333 4700 5085 5102 6554 6640 6652
Size: 2186 175 367 385 17 1452 86 12
Cuts at: 6652 6998 1972
Size: 346 1977
Fragments arranged by size:
2186 1977 1452 385 367 346 175 86
17 12
BstZ17I GTA'TAC
Cuts at: 4209 4209
Size: 7003
BtgI C'CryG_G
Cuts at: 513 1065 2015 2076 3960 4222 513
Size: 552 950 61 1884 262 3294
Fragments arranged by size:
3294 1884 950 552 262 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6264
Fragments arranged by size:
6264 683 56
BtsI GCAGTG_nn'
Cuts at: 2738 2865 2951 4519 5313 5333 2738
Size: 127 86 1568 794 20 4408
Fragments arranged by size:
4408 1568 794 127 86 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3685 3720 529
Size: 35 3121 35 3812
Fragments arranged by size:
3812 3121 35 35
DraI TTT'AAA
Cuts at: 2415 4090 4307 2415
Size: 1675 217 5111
Fragments arranged by size:
5111 1675 217
DraIII CAC_nnn'GTG
Cuts at: 2463 2463
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2699 6010 809
Size: 507 1383 3311 1802
Fragments arranged by size:
3311 1802 1383 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4450 5339 6968
Size: 54 1425 50 275 18 2620 889 1629
Cuts at: 6968 8
Size: 43
Fragments arranged by size:
2620 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4450 1537
Size: 275 2638 4090
Fragments arranged by size:
4090 2638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 2610 4933 1353
Size: 1080 177 2323 3423
Fragments arranged by size:
3423 2323 1080 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5590 5964 6110 387
Size: 1064 138 4001 374 146 1280
Fragments arranged by size:
4001 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2611 3066 3664 4014 5066 5663
Size: 393 557 422 455 598 350 1052 597
Cuts at: 5663 6450 6718 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1052 787 598 597 557 455 422
393 350 268
EcoRI G'AATT_C
Cuts at: 4312 4690 4312
Size: 378 6625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2723 2955 4058 2723
Size: 232 1103 5668
Fragments arranged by size:
5668 1103 232
FspI TGC'GCA
Cuts at: 5509 5509
Size: 7003
HaeII r_GCGC'y
Cuts at: 1678 3968 6150 1678
Size: 2290 2182 2531
Fragments arranged by size:
2531 2290 2182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2292 2324 2878
Size: 32 135 32 610 32 78 32 554
Cuts at: 2878 2910 3214 3246 3580 3612 3756 3788 5644
Size: 32 304 32 334 32 144 32 1856
Cuts at: 5644 5676 5718 5750 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1856 610 554 334 304 144 135
78 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4347 4593 669
Size: 988 281 2409 246 3079
Fragments arranged by size:
3079 2409 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7003
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KasI G'GCGC_C
Cuts at: 3964 3964
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 4602 4602
Size: 7003
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2819 3046 3331 4162 6117 6301 1305
Size: 1514 227 285 831 1955 184 2007
Fragments arranged by size:
2007 1955 1514 831 285 227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5181
Fragments arranged by size:
5181 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3044 4961 5320 5479 518
Size: 648 903 975 1917 359 159 2042
Fragments arranged by size:
2042 1917 975 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2428 3412 4419 5096 6244 6489 1606
Size: 508 314 984 1007 677 1148 245 2120
Fragments arranged by size:
2120 1148 1007 984 677 508 314 245
NarI GG'CG_CC
Cuts at: 3965 3965
Size: 7003
NcoI C'CATG_G
Cuts at: 513 1065 4222 513
Size: 552 3157 3294
Fragments arranged by size:
3294 3157 552
NdeI CA'TA_TG
Cuts at: 387 2398 2500 2677 3895 387
Size: 2011 102 177 1218 3495
Fragments arranged by size:
3495 2011 1218 177 102
NheI G'CTAG_C
Cuts at: 1052 3731 4232 1052
Size: 2679 501 3823
Fragments arranged by size:
3823 2679 501
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NsiI A_TGCA'T
Cuts at: 3780 3780
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 3336 3782 4361 5906 1132
Size: 97 2107 446 579 1545 2229
Fragments arranged by size:
2229 2107 1545 579 446 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7003
PciI A'CATG_T
Cuts at: 5902 5902
Size: 7003
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7003
PfoI T'CCnGG_A
Cuts at: 3483 3483
Size: 7003
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5072 5104 6585 6617 5072
Size: 32 1481 32 5458
Fragments arranged by size:
5458 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7003
PsiI TTA'TAA
Cuts at: 3300 4573 3300
Size: 1273 5730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2526 2558 2526
Size: 32 6971
PstI C_TGCA'G
Cuts at: 830 2745 3519 4355 830
Size: 1915 774 836 3478
Fragments arranged by size:
3478 1915 836 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2114 2428 3412 2114
Size: 314 984 5705
Fragments arranged by size:
5705 984 314
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 3052 3371 3986 5251 6963 3052
Size: 319 615 1265 1712 3092
Fragments arranged by size:
3092 1712 1265 615 319
SfcI C'TryA_G
Cuts at: 826 1045 2109 2741 3515 4351 5486 6167 6358
Size: 219 1064 632 774 836 1135 681 191
Cuts at: 6358 826
Size: 1471
Fragments arranged by size:
1471 1135 1064 836 774 681 632 219
191
SfoI GGC'GCC
Cuts at: 3966 3966
Size: 7003
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 5107 6008 6270 820
Size: 197 926 239 2925 901 262 1553
Fragments arranged by size:
2925 1553 926 901 262 239 197
SnaBI TAC'GTA
Cuts at: 493 3907 4794 493
Size: 3414 887 2702
Fragments arranged by size:
3414 2702 887
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 3782 4361 1132
Size: 2650 579 3774
Fragments arranged by size:
3774 2650 579
SspI AAT'ATT
Cuts at: 5 52 4927 5
Size: 47 4875 2081
Fragments arranged by size:
4875 2081 47
StuI AGG'CCT
Cuts at: 1517 3079 1517
Size: 1562 5441
StyI C'CwwG_G
Cuts at: 513 1065 3760 4222 4442 513
Size: 552 2695 462 220 3074
Fragments arranged by size:
3074 2695 552 462 220
SwaI ATTT'AAAT
Cuts at: 2415 2415
Size: 7003
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2962 5030 5047 5200 5385 5830
Size: 1255 450 932 2068 17 153 185 445
Cuts at: 5830 325
Size: 1498
Fragments arranged by size:
2068 1498 1255 932 450 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2282 3050 3369
Size: 274 80 33 51 1443 305 768 319
Cuts at: 3369 3984 3999 5249 6961 96
Size: 615 15 1250 1712 138
Fragments arranged by size:
1712 1443 1250 768 615 319 305 274
138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3924 3945 3956 3977 6963 6995 3924
Size: 21 11 21 2986 32 3932
Fragments arranged by size:
3932 2986 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 3674 1316
Size: 2358 4645
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7003
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 5132 5132
Size: 7003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BplI
BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI XcmI