Restriction map for
pFN21AB5771
(Circular) (Six-base) MAPSORT of: pFN21AB5771.seq Check: 4501 from: 1 to: 6904
pFN21AB5771 6904 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2432 3199 2432
Size: 767 6137
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4714 278
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 2925 4247 1937
Size: 816 172 1322 4594
Fragments arranged by size:
4594 1322 816 172
Acc65I G'GTAC_C
Cuts at: 2942 4225 2942
Size: 1283 5621
AclI AA'CG_TT
Cuts at: 1563 5031 5404 1563
Size: 3468 373 3063
Fragments arranged by size:
3468 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 3896 4967 6351 6619 1632
Size: 495 1769 1071 1384 268 1917
Fragments arranged by size:
1917 1769 1384 1071 495 268
AflII C'TTAA_G
Cuts at: 820 1017 2719 820
Size: 197 1702 5005
Fragments arranged by size:
5005 1702 197
AflIII A'CryG_T
Cuts at: 1203 5803 6780 1203
Size: 4600 977 1327
Fragments arranged by size:
4600 1327 977
AgeI A'CCGG_T
Cuts at: 5735 5735
Size: 6904
AhdI GACnn_n'nnGTC
Cuts at: 3538 5633 3538
Size: 2095 4809
AleI CACnn'nnGTG
Cuts at: 1166 3777 1166
Size: 2611 4293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3020 1184
Size: 1836 5068
AlwNI CAG_nnn'CTG
Cuts at: 3026 3909 4647 6219 3026
Size: 883 738 1572 3711
Fragments arranged by size:
3711 1572 883 738
ApaLI G'TGCA_C
Cuts at: 2978 4961 6117 2978
Size: 1983 1156 3765
Fragments arranged by size:
3765 1983 1156
ApoI r'AATT_y
Cuts at: 1493 2324 4213 4439 4591 6700 1493
Size: 831 1889 226 152 2109 1697
Fragments arranged by size:
2109 1889 1697 831 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5458 160
Size: 872 4426 1606
Fragments arranged by size:
4426 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
AvaI C'yCGr_G
Cuts at: 1870 1943 3159 3561 3743 4142 4229 1870
Size: 73 1216 402 182 399 87 4545
Fragments arranged by size:
4545 1216 402 399 182 87 73
BamHI G'GATC_C
Cuts at: 4234 4601 4234
Size: 367 6537
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 2942 3739 4225 5680
Size: 325 217 627 611 544 797 486 1455
Cuts at: 5680 618
Size: 1842
Fragments arranged by size:
1842 1455 797 627 611 544 486 325
217
BanII G_rGCy'C
Cuts at: 1391 4094 4152 4223 1391
Size: 2703 58 71 4072
Fragments arranged by size:
4072 2703 71 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 3144 3999 4114 928
Size: 971 779 466 855 115 3718
Fragments arranged by size:
3718 971 855 779 466 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5095 5129 1903
Size: 34 3158 34 3678
Fragments arranged by size:
3678 3158 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4796 6006 4796
Size: 1210 5694
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6904
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3079 3475 5515
Size: 107 122 71 631 605 1407 396 2040
Cuts at: 5515 136
Size: 1525
Fragments arranged by size:
2040 1525 1407 631 605 396 122 107
71
BglII A'GATC_T
Cuts at: 2664 3813 6899 2664
Size: 1149 3086 2669
Fragments arranged by size:
3086 2669 1149
BlpI GC'TnA_GC
Cuts at: 4321 4321
Size: 6904
Bme1580I G_kGCm'C
Cuts at: 1790 2403 2982 3960 4965 6121 1790
Size: 613 579 978 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 978 613 579
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6904
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3897 4635 5593
Size: 416 53 83 399 24 2480 738 958
Cuts at: 5593 442
Size: 1753
Fragments arranged by size:
2480 1753 958 738 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3110 1056
Size: 2054 4850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3018 3445 3538 3018
Size: 427 93 6384
Fragments arranged by size:
6384 427 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 2700 2882 3019 3073 5564 1239
Size: 1156 305 182 137 54 2491 2579
Fragments arranged by size:
2579 2491 1156 305 182 137 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5029 5894 6192 5029
Size: 865 298 5741
Fragments arranged by size:
5741 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3121 3597 3961 5567 882
Size: 617 1622 476 364 1606 2219
Fragments arranged by size:
2219 1622 1606 617 476 364
BsaAI yAC'GTr
Cuts at: 493 1204 2757 4098 4695 493
Size: 711 1553 1341 597 2702
Fragments arranged by size:
2702 1553 1341 711 597
BsaBI GATnn'nnATC
Cuts at: 2870 6898 2870
Size: 4028 2876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4711 5093 5728 275
Size: 53 83 186 936 3178 382 635 1451
Fragments arranged by size:
3178 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3759 5337 5735 6009 6156 6567 3759
Size: 1578 398 274 147 411 4096
Fragments arranged by size:
4096 1578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 2549 2579 2741
Size: 30 165 30 1023 30 93 30 162
Cuts at: 2741 2771 4049 4079 1178
Size: 30 1278 30 4003
Fragments arranged by size:
4003 1278 1023 165 162 93 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2098 2128 2162 3076 3514 3527 4093
Size: 829 83 30 34 914 438 13 566
Cuts at: 4093 4160 1186
Size: 67 3930
Fragments arranged by size:
3930 914 829 566 438 83 67 34
30 13
BseYI C'CCAG_C
Cuts at: 3147 3379 3497 4070 4168 4173 6107 6603 3147
Size: 232 118 573 98 5 1934 496 3448
Fragments arranged by size:
3448 1934 573 496 232 118 98 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2044 3373 3895 1163
Size: 573 308 1329 522 4172
Fragments arranged by size:
4172 1329 573 522 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4354 5115 6143 1540
Size: 275 186 1717 636 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 636 275 186
BsiHKAI G_wGCw'C
Cuts at: 2982 3804 4094 4103 4965 5050 6121 2982
Size: 822 290 9 862 85 1071 3765
Fragments arranged by size:
3765 1071 862 822 290 85 9
BsmI GAATG_Cn'
Cuts at: 4415 4508 4415
Size: 93 6811
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2982 3804 3960 4094 4103 4152
Size: 399 613 579 822 156 134 9 49
Cuts at: 4152 4223 4965 5050 6121 1391
Size: 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 822 742 613 579 399 156
134 85 71 49 9
BspEI T'CCGG_A
Cuts at: 6567 6567
Size: 6904
BspHI T'CATG_A
Cuts at: 4129 4793 6523 4129
Size: 664 1730 4510
Fragments arranged by size:
4510 1730 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2658 3199 3384 3450 3462 4259 844
Size: 1588 226 541 185 66 12 797 3489
Fragments arranged by size:
3489 1588 797 541 226 185 66 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2485 4791 1394
Size: 429 662 2306 3507
Fragments arranged by size:
3507 2306 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5399 5573 65
Size: 1183 4151 174 1396
Fragments arranged by size:
4151 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2242 2496 2833 5548 5735 1700
Size: 110 432 254 337 2715 187 2869
Fragments arranged by size:
2869 2715 432 337 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 3924 96
Size: 2478 1350 3076
Fragments arranged by size:
3076 2478 1350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6904
BssSI C'ACGA_G
Cuts at: 4964 5976 4964
Size: 1012 5892
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 3955 794
Size: 1445 1716 3743
Fragments arranged by size:
3743 1716 1445
BstBI TT'CG_AA
Cuts at: 5708 5708
Size: 6904
BstEII G'GTnAC_C
Cuts at: 1184 3884 1184
Size: 2700 4204
BstXI CCAn_nnnn'nTGG
Cuts at: 2345 3535 4054 2345
Size: 1190 519 5195
Fragments arranged by size:
5195 1190 519
BstYI r'GATC_y
Cuts at: 1972 2664 3762 3813 4234 4601 4986 5003 6455
Size: 692 1098 51 421 367 385 17 1452
Cuts at: 6455 6541 6553 6899 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1098 692 421 385 367 346
86 51 17 12
BstZ17I GTA'TAC
Cuts at: 2754 2754
Size: 6904
Bsu36I CC'TnA_GG
Cuts at: 4105 4105
Size: 6904
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6165
Fragments arranged by size:
6165 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 2297 2844 3960 4420 5214 5234 2244
Size: 53 547 1116 460 794 20 3914
Fragments arranged by size:
3914 1116 794 547 460 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6904
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6869
DraI TTT'AAA
Cuts at: 4208 4208
Size: 6904
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3170 5911 809
Size: 507 1854 2741 1802
Fragments arranged by size:
2741 1854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2988 3071 3715
Size: 54 1425 50 275 18 1158 83 644
Cuts at: 3715 4351 5240 6869 8
Size: 636 889 1629 43
Fragments arranged by size:
1629 1425 1158 889 644 636 275 83
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 4351 1537
Size: 275 1903 636 4090
Fragments arranged by size:
4090 1903 636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2146 2820 2930 2945 4834 1353
Size: 680 113 674 110 15 1889 3423
Fragments arranged by size:
3423 1889 680 674 113 110 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4203 5491 5865 6011 387
Size: 1064 138 2614 1288 374 146 1280
Fragments arranged by size:
2614 1288 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4092 4092
Size: 6904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2395 2700 2882 3019 3073 3896
Size: 393 495 268 305 182 137 54 823
Cuts at: 3896 4967 5564 6351 6619 1239
Size: 1071 597 787 268 1524
Fragments arranged by size:
1524 1071 823 787 597 495 393 305
268 268 182 137 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3191
Size: 6904
EcoO109I rG'GnC_Cy
Cuts at: 2283 2886 3384 3468 3532 2283
Size: 603 498 84 64 5655
Fragments arranged by size:
5655 603 498 84 64
EcoRI G'AATT_C
Cuts at: 2324 4213 4591 2324
Size: 1889 378 4637
Fragments arranged by size:
4637 1889 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 6904
FspI TGC'GCA
Cuts at: 3008 5410 3008
Size: 2402 4502
HaeII r_GCGC'y
Cuts at: 1678 6051 1678
Size: 4373 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2123 2152 2155 3308
Size: 32 135 32 548 3 29 3 1153
Cuts at: 3308 3340 3524 3556 3773 3805 3837 4049 4081
Size: 32 184 32 217 32 32 212 32
Cuts at: 4081 5545 5577 5619 5651 1373
Size: 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 1153 548 217 212 184 135
42 32 32 32 32 32 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 4248 4494 669
Size: 988 281 867 1443 246 3079
Fragments arranged by size:
3079 1443 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6904
HpaI GTT'AAC
Cuts at: 4494 4494
Size: 6904
KpnI G_GTAC'C
Cuts at: 2946 4229 2946
Size: 1283 5621
MfeI C'AATT_G
Cuts at: 4503 4503
Size: 6904
MluI A'CGCG_T
Cuts at: 6780 6780
Size: 6904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3270 4047 6018 6202 1305
Size: 1965 777 1971 184 2007
Fragments arranged by size:
2007 1971 1965 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2990 10
Size: 54 1425 343 1158 3924
Fragments arranged by size:
3924 1425 1158 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2618 3777 3932 4862 5221 5380
Size: 648 1218 234 1159 155 930 359 159
Cuts at: 5380 518
Size: 2042
Fragments arranged by size:
2042 1218 1159 930 648 359 234 159
155
MspA1I CmG'CkG
Cuts at: 1606 2470 3985 4138 4320 4997 6145 6390 1606
Size: 864 1515 153 182 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 864 677 245 182 153
NaeI GCC'GGC
Cuts at: 2244 2835 2244
Size: 591 6313
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6352
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6904
NgoMIV G'CCGG_C
Cuts at: 2242 2833 2242
Size: 591 6313
NheI G'CTAG_C
Cuts at: 1052 3106 1052
Size: 2054 4850
NotI GC'GGCC_GC
Cuts at: 4351 4351
Size: 6904
NspI r_CATG'y
Cuts at: 1132 1229 2237 3953 4262 5807 1132
Size: 97 1008 1716 309 1545 2229
Fragments arranged by size:
2229 1716 1545 1008 309 97
PasI CC'CwG_GG
Cuts at: 1348 2542 3187 3473 1348
Size: 1194 645 286 4779
Fragments arranged by size:
4779 1194 645 286
PciI A'CATG_T
Cuts at: 5803 5803
Size: 6904
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6904
PmeI GTTT'AAAC
Cuts at: 4208 4208
Size: 6904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4973 5005 6486 6518 4973
Size: 32 1481 32 5359
Fragments arranged by size:
5359 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 3532 2283
Size: 1249 5655
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6904
PsiI TTA'TAA
Cuts at: 4474 4474
Size: 6904
PstI C_TGCA'G
Cuts at: 830 3459 4256 830
Size: 2629 797 3478
Fragments arranged by size:
3478 2629 797
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6904
PvuII CAG'CTG
Cuts at: 2470 3985 2470
Size: 1515 5389
SacI G_AGCT'C
Cuts at: 4094 4094
Size: 6904
SalI G'TCGA_C
Cuts at: 1936 4246 1936
Size: 2310 4594
SanDI GG'GwC_CC
Cuts at: 2283 2283
Size: 6904
SbfI CC_TGCA'GG
Cuts at: 3459 4256 3459
Size: 797 6107
ScaI AGT'ACT
Cuts at: 4064 5152 6864 4064
Size: 1088 1712 4104
Fragments arranged by size:
4104 1712 1088
SexAI A'CCwGG_T
Cuts at: 3534 3552 3534
Size: 18 6886
SfcI C'TryA_G
Cuts at: 826 1045 3455 4252 5387 6068 6259 826
Size: 219 2410 797 1135 681 191 1471
Fragments arranged by size:
2410 1471 1135 797 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6904
SmaI CCC'GGG
Cuts at: 1872 4231 1872
Size: 2359 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2719 5008 5909 6171 820
Size: 197 926 776 2289 901 262 1553
Fragments arranged by size:
2289 1553 926 901 776 262 197
SnaBI TAC'GTA
Cuts at: 493 4695 493
Size: 4202 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6904
SphI G_CATG'C
Cuts at: 1132 2237 3953 4262 1132
Size: 1105 1716 309 3774
Fragments arranged by size:
3774 1716 1105 309
SspI AAT'ATT
Cuts at: 5 52 4828 5
Size: 47 4776 2081
Fragments arranged by size:
4776 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6904
StyI C'CwwG_G
Cuts at: 513 1065 3432 3528 4343 513
Size: 552 2367 96 815 3074
Fragments arranged by size:
3074 2367 815 552 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3785 4182 4931 4948 5101 5286
Size: 1255 1325 880 397 749 17 153 185
Cuts at: 5286 5731 325
Size: 445 1498
Fragments arranged by size:
1498 1325 1255 880 749 445 397 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3924 4062
Size: 274 80 33 51 1443 597 1350 138
Cuts at: 4062 5150 6862 96
Size: 1088 1712 138
Fragments arranged by size:
1712 1443 1350 1088 597 274 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 2741 2773 6864 6896 2549
Size: 32 160 32 4091 32 2557
Fragments arranged by size:
4091 2557 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6904
XbaI T'CTAG_A
Cuts at: 4240 4240
Size: 6904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 3622 4181 2533
Size: 1089 559 5256
Fragments arranged by size:
5256 1089 559
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6904
XmaI C'CCGG_G
Cuts at: 1870 4229 1870
Size: 2359 4545
XmnI GAAnn'nnTTC
Cuts at: 5033 5033
Size: 6904
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4712 276
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI