Restriction map for
pFN21AB5770
(Circular) (Six-base) MAPSORT of: pFN21AB5770.seq Check: 3222 from: 1 to: 6988
pFN21AB5770 6988 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4014 4014
Size: 6988
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4798 278
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2296 4331 1937
Size: 359 2035 4594
Fragments arranged by size:
4594 2035 359
Acc65I G'GTAC_C
Cuts at: 3826 4309 3826
Size: 483 6505
AclI AA'CG_TT
Cuts at: 1563 5115 5488 1563
Size: 3552 373 3063
Fragments arranged by size:
3552 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 3281 4067 5051 6435 6703 1632
Size: 421 1228 786 984 1384 268 1917
Fragments arranged by size:
1917 1384 1228 984 786 421 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6791
AflIII A'CryG_T
Cuts at: 1203 2682 3882 5887 6864 1203
Size: 1479 1200 2005 977 1327
Fragments arranged by size:
2005 1479 1327 1200 977
AgeI A'CCGG_T
Cuts at: 5819 5819
Size: 6988
AhdI GACnn_n'nnGTC
Cuts at: 3916 4150 5717 3916
Size: 234 1567 5187
Fragments arranged by size:
5187 1567 234
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6988
AlwNI CAG_nnn'CTG
Cuts at: 2614 3122 4731 6303 2614
Size: 508 1609 1572 3299
Fragments arranged by size:
3299 1609 1572 508
ApaLI G'TGCA_C
Cuts at: 5045 6201 5045
Size: 1156 5832
ApoI r'AATT_y
Cuts at: 1493 2632 2879 2916 2930 3309 3602 3625 3668
Size: 1139 247 37 14 379 293 23 43
Cuts at: 3668 3851 4180 4297 4523 4675 6784 1493
Size: 183 329 117 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 379 329 293 247 226
183 152 117 43 37 23 14
AseI AT'TA_AT
Cuts at: 160 1032 5542 160
Size: 872 4510 1606
Fragments arranged by size:
4510 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
AvaI C'yCGr_G
Cuts at: 1870 1943 4313 1870
Size: 73 2370 4545
Fragments arranged by size:
4545 2370 73
BamHI G'GATC_C
Cuts at: 4318 4685 4318
Size: 367 6621
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3826 4309 5764 618
Size: 325 217 627 2039 483 1455 1842
Fragments arranged by size:
2039 1842 1455 627 483 325 217
BanII G_rGCy'C
Cuts at: 1391 3682 4307 1391
Size: 2291 625 4072
Fragments arranged by size:
4072 2291 625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3517 928
Size: 971 1618 4399
Fragments arranged by size:
4399 1618 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5179 5213 1903
Size: 34 50 34 3158 34 3678
Fragments arranged by size:
3678 3158 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4880 6090 4880
Size: 1210 5778
BclI T'GATC_A
Cuts at: 1548 2149 1548
Size: 601 6387
BfrBI ATG'CAT
Cuts at: 4040 4040
Size: 6988
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5599 136
Size: 107 122 71 631 605 3927 1525
Fragments arranged by size:
3927 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2724 6983 2724
Size: 4259 2729
BlpI GC'TnA_GC
Cuts at: 3924 4405 3924
Size: 481 6507
Bme1580I G_kGCm'C
Cuts at: 1790 5049 6205 1790
Size: 3259 1156 2573
Fragments arranged by size:
3259 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4719 5677 442
Size: 416 53 83 399 24 3302 958 1753
Fragments arranged by size:
3302 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2580 2639 3488 5648 1239
Size: 1341 59 849 2160 2579
Fragments arranged by size:
2579 2160 1341 849 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5113 5978 6276 5113
Size: 865 298 5825
Fragments arranged by size:
5825 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 2591 5651 882
Size: 617 687 405 3060 2219
Fragments arranged by size:
3060 2219 687 617 405
BsaAI yAC'GTr
Cuts at: 493 1204 4779 493
Size: 711 3575 2702
Fragments arranged by size:
3575 2702 711
BsaBI GATnn'nnATC
Cuts at: 6982 6982
Size: 6988
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4795 5177 5812 275
Size: 53 83 186 936 3262 382 635 1451
Fragments arranged by size:
3262 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5421 5819 6093 6240 6651 5421
Size: 398 274 147 411 5758
Fragments arranged by size:
5758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3123 3153 1178
Size: 30 165 30 1720 30 5013
Fragments arranged by size:
5013 1720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6988
BseYI C'CCAG_C
Cuts at: 2423 6191 6687 2423
Size: 3768 496 2724
Fragments arranged by size:
3768 2724 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2017 3441 1163
Size: 573 281 1424 4710
Fragments arranged by size:
4710 1424 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3537 4438 5199 6227 1540
Size: 275 186 1536 901 761 1028 2301
Fragments arranged by size:
2301 1536 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2048 2829 3682 3759 5049 5134 6205 2048
Size: 781 853 77 1290 85 1071 2831
Fragments arranged by size:
2831 1290 1071 853 781 85 77
BsmI GAATG_Cn'
Cuts at: 3559 4499 4592 3559
Size: 940 93 5955
Fragments arranged by size:
5955 940 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2829 3682 3759 4307 5049 5134
Size: 399 258 781 853 77 548 742 85
Cuts at: 5134 6205 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 853 781 742 548 399 258
85 77
BspEI T'CCGG_A
Cuts at: 6651 6651
Size: 6988
BspHI T'CATG_A
Cuts at: 3598 4877 6607 3598
Size: 1279 1730 3979
Fragments arranged by size:
3979 1730 1279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4014 4343 844
Size: 3170 329 3489
Fragments arranged by size:
3489 3170 329
BsrBI CCG'CTC
Cuts at: 1394 1823 4090 4875 1394
Size: 429 2267 785 3507
Fragments arranged by size:
3507 2267 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2416 4040 5483 5657 65
Size: 1183 1168 1624 1443 174 1396
Fragments arranged by size:
1624 1443 1396 1183 1168 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3536 5632 5819 1700
Size: 110 1726 2096 187 2869
Fragments arranged by size:
2869 2096 1726 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6988
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6988
BssSI C'ACGA_G
Cuts at: 5048 6060 5048
Size: 1012 5976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6988
BstBI TT'CG_AA
Cuts at: 5792 5792
Size: 6988
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6988
BstXI CCAn_nnnn'nTGG
Cuts at: 3628 3628
Size: 6988
BstYI r'GATC_y
Cuts at: 1972 2724 4318 4685 5070 5087 6539 6625 6637
Size: 752 1594 367 385 17 1452 86 12
Cuts at: 6637 6983 1972
Size: 346 1977
Fragments arranged by size:
1977 1594 1452 752 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2078 513
Size: 552 1013 5423
Fragments arranged by size:
5423 1013 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6249
Fragments arranged by size:
6249 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 3424 3429 4504 5298 5318 2612
Size: 812 5 1075 794 20 4282
Fragments arranged by size:
4282 1075 812 794 20 5
ClaI AT'CG_AT
Cuts at: 1554 2170 3181 1554
Size: 616 1011 5361
Fragments arranged by size:
5361 1011 616
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6953
DraI TTT'AAA
Cuts at: 4292 4292
Size: 6988
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4110 5995 809
Size: 507 2794 1885 1802
Fragments arranged by size:
2794 1885 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2983 3397 3534
Size: 54 1425 50 275 18 1153 414 137
Cuts at: 3534 4435 5324 6953 8
Size: 901 889 1629 43
Fragments arranged by size:
1629 1425 1153 901 889 414 275 137
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3534 4435 1537
Size: 275 1722 901 4090
Fragments arranged by size:
4090 1722 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2427 2472 2637 2817 3088 4918 1353
Size: 683 391 45 165 180 271 1830 3423
Fragments arranged by size:
3423 1830 683 391 271 180 165 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5575 5949 6095 387
Size: 1064 138 3986 374 146 1280
Fragments arranged by size:
3986 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3680 3680
Size: 6988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2580 2639 3281 3488 4067 5051
Size: 393 421 527 59 642 207 579 984
Cuts at: 5051 5648 6435 6703 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 984 787 642 597 579 527 421
393 268 207 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2068 2873 2068
Size: 805 6183
EcoO109I rG'GnC_Cy
Cuts at: 2083 4051 2083
Size: 1968 5020
EcoRI G'AATT_C
Cuts at: 4297 4675 4297
Size: 378 6610
FspI TGC'GCA
Cuts at: 5494 5494
Size: 6988
HaeII r_GCGC'y
Cuts at: 1678 6135 1678
Size: 4457 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3770 3802 3902 3934 3965
Size: 32 135 32 2198 32 100 32 31
Cuts at: 3965 3997 4136 4168 5629 5661 5703 5735 1373
Size: 32 139 32 1461 32 42 32 2626
Fragments arranged by size:
2626 2198 1461 139 135 100 42 32
32 32 32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2680 4332 4578 669
Size: 988 281 742 1652 246 3079
Fragments arranged by size:
3079 1652 988 742 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6988
HpaI GTT'AAC
Cuts at: 2680 4578 2680
Size: 1898 5090
KpnI G_GTAC'C
Cuts at: 3830 4313 3830
Size: 483 6505
MfeI C'AATT_G
Cuts at: 3153 4587 3153
Size: 1434 5554
MluI A'CGCG_T
Cuts at: 6864 6864
Size: 6988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2606 3843 3917 6102 6286 1305
Size: 774 527 1237 74 2185 184 2007
Fragments arranged by size:
2185 2007 1237 774 527 184 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2985 10
Size: 54 1425 343 1153 4013
Fragments arranged by size:
4013 1425 1153 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4946 5305 5464 518
Size: 648 3780 359 159 2042
Fragments arranged by size:
3780 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 3923 4404 5081 6229 6474 1606
Size: 474 1843 481 677 1148 245 2120
Fragments arranged by size:
2120 1843 1148 677 481 474 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6436
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6988
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6988
NotI GC'GGCC_GC
Cuts at: 4435 4435
Size: 6988
NsiI A_TGCA'T
Cuts at: 4042 4042
Size: 6988
NspI r_CATG'y
Cuts at: 1132 1229 3886 4346 5891 1132
Size: 97 2657 460 1545 2229
Fragments arranged by size:
2657 2229 1545 460 97
PasI CC'CwG_GG
Cuts at: 1348 3662 1348
Size: 2314 4674
PciI A'CATG_T
Cuts at: 3882 5887 3882
Size: 2005 4983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6988
PmeI GTTT'AAAC
Cuts at: 4292 4292
Size: 6988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3783 3815 5057 5089 6570 6602 3783
Size: 32 1242 32 1481 32 4169
Fragments arranged by size:
4169 1481 1242 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 4051 2083
Size: 1968 5020
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6988
PsiI TTA'TAA
Cuts at: 4558 4558
Size: 6988
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3207 3239 3207
Size: 32 6956
PstI C_TGCA'G
Cuts at: 830 3419 3553 4340 830
Size: 2589 134 787 3478
Fragments arranged by size:
3478 2589 787 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6988
PvuII CAG'CTG
Cuts at: 3923 3923
Size: 6988
SacI G_AGCT'C
Cuts at: 3682 3682
Size: 6988
SacII CC_GC'GG
Cuts at: 2081 2081
Size: 6988
SalI G'TCGA_C
Cuts at: 1936 4330 1936
Size: 2394 4594
SanDI GG'GwC_CC
Cuts at: 2083 2083
Size: 6988
SapI GCTCTTCn'nnn_
Cuts at: 2036 2817 3088 2036
Size: 781 271 5936
Fragments arranged by size:
5936 781 271
SbfI CC_TGCA'GG
Cuts at: 4340 4340
Size: 6988
ScaI AGT'ACT
Cuts at: 3555 5236 6948 3555
Size: 1681 1712 3595
Fragments arranged by size:
3595 1712 1681
SfcI C'TryA_G
Cuts at: 826 1045 2116 2206 3415 3549 4257 4336 5471
Size: 219 1071 90 1209 134 708 79 1135
Cuts at: 5471 6152 6343 826
Size: 681 191 1471
Fragments arranged by size:
1471 1209 1135 1071 708 681 219 191
134 90 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6988
SmaI CCC'GGG
Cuts at: 1872 4315 1872
Size: 2443 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5092 5993 6255 820
Size: 197 926 3149 901 262 1553
Fragments arranged by size:
3149 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4779 493
Size: 4286 2702
SpeI A'CTAG_T
Cuts at: 152 2213 152
Size: 2061 4927
SphI G_CATG'C
Cuts at: 1132 4346 1132
Size: 3214 3774
SspI AAT'ATT
Cuts at: 5 52 2259 3373 3586 4912 5
Size: 47 2207 1114 213 1326 2081
Fragments arranged by size:
2207 2081 1326 1114 213 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6988
StyI C'CwwG_G
Cuts at: 513 1065 4427 513
Size: 552 3362 3074
Fragments arranged by size:
3362 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2095 5015 5032 5185 5370 5815 325
Size: 1255 515 2920 17 153 185 445 1498
Fragments arranged by size:
2920 1498 1255 515 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3409 3553 5234
Size: 274 80 33 51 1443 1432 144 1681
Cuts at: 5234 6946 96
Size: 1712 138
Fragments arranged by size:
1712 1681 1443 1432 274 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 6948 6980 2768
Size: 32 4148 32 2776
Fragments arranged by size:
4148 2776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6988
XbaI T'CTAG_A
Cuts at: 3038 4324 3038
Size: 1286 5702
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6988
XmaI C'CCGG_G
Cuts at: 1870 4313 1870
Size: 2443 4545
XmnI GAAnn'nnTTC
Cuts at: 2646 5117 2646
Size: 2471 4517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4796 276
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI RsrII SexAI SfiI
SfoI SrfI SwaI XcmI