Restriction map for
pFN21AB5767
(Circular) (Six-base) MAPSORT of: pFN21AB5767.seq Check: 7810 from: 1 to: 7141
pFN21AB5767 7141 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 2661 3958 4087 2391
Size: 270 1297 129 5445
Fragments arranged by size:
5445 1297 270 129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2094 2595 4951 278
Size: 53 83 186 936 558 501 2356 2468
Fragments arranged by size:
2468 2356 936 558 501 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 4484 1937
Size: 157 2390 4594
Fragments arranged by size:
4594 2390 157
Acc65I G'GTAC_C
Cuts at: 4462 4462
Size: 7141
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 3911 5204 6588 6856 1632
Size: 755 1524 1293 1384 268 1917
Fragments arranged by size:
1917 1524 1384 1293 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6944
AflIII A'CryG_T
Cuts at: 1203 2928 6040 7017 1203
Size: 1725 3112 977 1327
Fragments arranged by size:
3112 1725 1327 977
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7141
AlwNI CAG_nnn'CTG
Cuts at: 2495 2860 3178 3481 4884 6456 2495
Size: 365 318 303 1403 1572 3180
Fragments arranged by size:
3180 1572 1403 365 318 303
ApaI G_GGCC'C
Cuts at: 2325 2602 2671 2845 3220 3637 4002 4351 2325
Size: 277 69 174 375 417 365 349 5115
Fragments arranged by size:
5115 417 375 365 349 277 174 69
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 2737 3011 3167 4450 4676 4828 6937 1493
Size: 1244 274 156 1283 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 1244 274 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 2543 2838 2952 3468 4466 1870
Size: 73 600 295 114 516 998 4545
Fragments arranged by size:
4545 998 600 516 295 114 73
AvrII C'CTAG_G
Cuts at: 3226 3226
Size: 7141
BamHI G'GATC_C
Cuts at: 3337 3432 4077 4471 4838 3337
Size: 95 645 394 367 5640
Fragments arranged by size:
5640 645 394 367 95
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3017 3392 3809 4189 4462
Size: 325 217 627 1230 375 417 380 273
Cuts at: 4462 5917 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1230 627 417 380 375 325
273 217
BanII G_rGCy'C
Cuts at: 1391 2294 2325 2376 2602 2671 2845 3220 3366
Size: 903 31 51 226 69 174 375 146
Cuts at: 3366 3637 4002 4176 4351 4460 1391
Size: 271 365 174 175 109 4072
Fragments arranged by size:
4072 903 375 365 271 226 175 174
174 146 109 69 51 31
BbeI G_GCGC'C
Cuts at: 3021 3396 3021
Size: 375 6766
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3403 928
Size: 971 1504 4666
Fragments arranged by size:
4666 1504 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2534 2568 2819 2853 5332 5366 1903
Size: 34 597 34 251 34 2479 34 3678
Fragments arranged by size:
3678 2479 597 251 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3532 4282 5033 6243 3532
Size: 750 751 1210 4430
Fragments arranged by size:
4430 1210 751 750
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 4187 5752
Size: 107 122 71 631 605 2325 190 1565
Cuts at: 5752 136
Size: 1525
Fragments arranged by size:
2325 1565 1525 631 605 190 122 107
71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 4558 4558
Size: 7141
Bme1580I G_kGCm'C
Cuts at: 1790 2325 2602 2671 2845 3220 3637 4002 4351
Size: 535 277 69 174 375 417 365 349
Cuts at: 4351 5202 6358 1790
Size: 851 1156 2573
Fragments arranged by size:
2573 1156 851 535 417 375 365 349
277 174 69
BmgBI CAC'GTC
Cuts at: 1318 2118 1318
Size: 800 6341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 3089 4872
Size: 416 53 83 399 24 911 761 1783
Cuts at: 4872 5830 442
Size: 958 1753
Fragments arranged by size:
1783 1753 958 911 761 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 7141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2348 2426 2936 3152 3234 3566 3886
Size: 923 186 78 510 216 82 332 320
Cuts at: 3886 3953 4055 4279 4388 5801 1239
Size: 67 102 224 109 1413 2579
Fragments arranged by size:
2579 1413 923 510 332 320 224 216
186 109 102 82 78 67
Bpu10I CC'TnA_GC
Cuts at: 4194 4194
Size: 7141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5266 6131 6429 5266
Size: 865 298 5978
Fragments arranged by size:
5978 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 5804 882
Size: 617 1413 2892 2219
Fragments arranged by size:
2892 2219 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 4932 493
Size: 711 3728 2702
Fragments arranged by size:
3728 2702 711
BsaBI GATnn'nnATC
Cuts at: 7135 7135
Size: 7141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2592 3018 3393
Size: 53 83 186 936 558 501 426 375
Cuts at: 3393 4049 4948 5330 5965 275
Size: 656 899 382 635 1451
Fragments arranged by size:
1451 936 899 656 635 558 501 426
382 375 186 83 53
BsaWI w'CCGG_w
Cuts at: 5574 5972 6246 6393 6804 5574
Size: 398 274 147 411 5911
Fragments arranged by size:
5911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 2981 3011 3053
Size: 30 165 30 753 30 795 30 42
Cuts at: 3053 3083 3232 3251 3262 3281 1178
Size: 30 149 19 11 19 5038
Fragments arranged by size:
5038 795 753 165 149 42 30 30
30 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2423 2521 2837 3272 1186
Size: 1159 78 98 316 435 5055
Fragments arranged by size:
5055 1159 435 316 98 78
BseYI C'CCAG_C
Cuts at: 2623 2968 3714 3914 3919 3923 4092 6344 6840
Size: 345 746 200 5 4 169 2252 496
Cuts at: 6840 2623
Size: 2924
Fragments arranged by size:
2924 2252 746 496 345 200 169 5
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 5665
Fragments arranged by size:
5665 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4591 5352 6380 1540
Size: 275 186 2590 761 1028 2301
Fragments arranged by size:
2590 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4436 5202 5287 6358 4436
Size: 766 85 1071 5219
Fragments arranged by size:
5219 1071 766 85
BsmI GAATG_Cn'
Cuts at: 2386 4374 4652 4745 2386
Size: 1988 278 93 4782
Fragments arranged by size:
4782 1988 278 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2325 2376 2602 2671 2845 3220
Size: 399 504 31 51 226 69 174 375
Cuts at: 3220 3366 3637 4002 4176 4351 4436 4460 5202
Size: 146 271 365 174 175 85 24 742
Cuts at: 5202 5287 6358 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 375 365 271
226 175 174 174 146 85 85 69
51 31 24
BspEI T'CCGG_A
Cuts at: 6804 6804
Size: 7141
BspHI T'CATG_A
Cuts at: 5030 6760 5030
Size: 1730 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2661 3528 3958 4087 4496 844
Size: 1547 270 867 430 129 409 3489
Fragments arranged by size:
3489 1547 867 430 409 270 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2335 5028 1394
Size: 429 512 2693 3507
Fragments arranged by size:
3507 2693 512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5636 5810 65
Size: 1183 4388 174 1396
Fragments arranged by size:
4388 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5785 5972 1700
Size: 110 3975 187 2869
Fragments arranged by size:
3975 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 5201 6213 5201
Size: 1012 6129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3529 794
Size: 2735 4406
BstBI TT'CG_AA
Cuts at: 5945 5945
Size: 7141
BstEII G'GTnAC_C
Cuts at: 1184 4300 1184
Size: 3116 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2318 2623 2224
Size: 94 305 6742
Fragments arranged by size:
6742 305 94
BstYI r'GATC_y
Cuts at: 1972 2265 2400 2991 3337 3432 4077 4329 4471
Size: 293 135 591 346 95 645 252 142
Cuts at: 4471 4838 5223 5240 6692 6778 6790 7136 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 645 591 385 367 346 346
293 252 142 135 95 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2246 2901 3993 4071 2246
Size: 655 1092 78 5316
Fragments arranged by size:
5316 1092 655 78
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 3056 3198 3306 3358 4176
Size: 552 939 35 1017 142 108 52 818
Cuts at: 4176 4264 513
Size: 88 3390
Fragments arranged by size:
3390 1017 939 818 552 142 108 88
52 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3548 487
Size: 683 56 2322 4080
Fragments arranged by size:
4080 2322 683 56
BtsI GCAGTG_nn'
Cuts at: 2610 3296 3785 3863 4657 5451 5471 2610
Size: 686 489 78 794 794 20 4280
Fragments arranged by size:
4280 794 794 686 489 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 4445 4445
Size: 7141
DraIII CAC_nnn'GTG
Cuts at: 3007 3007
Size: 7141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3154 6148 809
Size: 507 1838 2994 1802
Fragments arranged by size:
2994 1838 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2499 3825
Size: 54 1425 50 275 18 297 372 1326
Cuts at: 3825 4179 4588 5477 7106 8
Size: 354 409 889 1629 43
Fragments arranged by size:
1629 1425 1326 889 409 372 354 297
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2681 2777 3060 3084 5071 1353
Size: 1328 96 283 24 1987 3423
Fragments arranged by size:
3423 1987 1328 283 96 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5728 6102 6248 387
Size: 1064 138 4139 374 146 1280
Fragments arranged by size:
4139 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2348 2387 2426 2936 3152 3234
Size: 393 530 186 39 39 510 216 82
Cuts at: 3234 3566 3886 3911 3953 4055 4279 4388 5204
Size: 332 320 25 42 102 224 109 816
Cuts at: 5204 5801 6588 6856 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 530 510 393 332
320 268 224 216 186 109 102 82
42 39 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2180 2180
Size: 7141
EcoO109I rG'GnC_Cy
Cuts at: 2035 2079 2322 2599 2667 2668 2842 2889 2979
Size: 44 243 277 68 1 174 47 90
Cuts at: 2979 3127 3217 3281 3989 3998 4347 2035
Size: 148 90 64 708 9 349 4829
Fragments arranged by size:
4829 708 349 277 243 174 148 90
90 68 64 47 44 9 1
EcoRI G'AATT_C
Cuts at: 3167 4450 4828 3167
Size: 1283 378 5480
Fragments arranged by size:
5480 1283 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 7141
FspI TGC'GCA
Cuts at: 5647 5647
Size: 7141
HaeII r_GCGC'y
Cuts at: 1678 2012 3021 3396 3736 6288 1678
Size: 334 1009 375 340 2552 2531
Fragments arranged by size:
2552 2531 1009 375 340 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2758 2790 3251
Size: 32 135 32 584 32 570 32 461
Cuts at: 3251 3283 3335 3367 3547 3579 4347 4379 5782
Size: 32 52 32 180 32 768 32 1403
Cuts at: 5782 5814 5856 5888 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1403 768 584 570 461 180 135
52 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4159 4485 4731 669
Size: 988 281 2221 326 246 3079
Fragments arranged by size:
3079 2221 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7141
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KasI G'GCGC_C
Cuts at: 3017 3392 3017
Size: 375 6766
KpnI G_GTAC'C
Cuts at: 4466 4466
Size: 7141
MfeI C'AATT_G
Cuts at: 4740 4740
Size: 7141
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6255 6439 1305
Size: 4950 184 2007
Fragments arranged by size:
4950 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 10
Size: 54 1425 343 669 4650
Fragments arranged by size:
4650 1425 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 5099 5458 5617 518
Size: 648 1051 2882 359 159 2042
Fragments arranged by size:
2882 2042 1051 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2857 3331 3664 3871 3923 3940 4557
Size: 435 816 474 333 207 52 17 617
Cuts at: 4557 5234 6382 6627 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 617 474 435 333
245 207 52 17
NarI GG'CG_CC
Cuts at: 3018 3393 3018
Size: 375 6766
NcoI C'CATG_G
Cuts at: 513 1065 2004 3198 4264 513
Size: 552 939 1194 1066 3390
Fragments arranged by size:
3390 1194 1066 939 552
NdeI CA'TA_TG
Cuts at: 387 3387 387
Size: 3000 4141
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7141
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 2932 4165 4499 6044 1132
Size: 97 1703 1233 334 1545 2229
Fragments arranged by size:
2229 1703 1545 1233 334 97
PasI CC'CwG_GG
Cuts at: 1348 2156 2602 1348
Size: 808 446 5887
Fragments arranged by size:
5887 808 446
PciI A'CATG_T
Cuts at: 2928 6040 2928
Size: 3112 4029
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3178 3312 3481 1108
Size: 2070 134 169 4768
Fragments arranged by size:
4768 2070 169 134
PfoI T'CCnGG_A
Cuts at: 3120 3171 4365 3120
Size: 51 1194 5896
Fragments arranged by size:
5896 1194 51
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2079 2889 2979 2035
Size: 44 810 90 6197
Fragments arranged by size:
6197 810 90 44
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7141
PsiI TTA'TAA
Cuts at: 4711 4711
Size: 7141
PspOMI G'GGCC_C
Cuts at: 2321 2598 2667 2841 3216 3633 3998 4347 2321
Size: 277 69 174 375 417 365 349 5115
Fragments arranged by size:
5115 417 375 365 349 277 174 69
PstI C_TGCA'G
Cuts at: 830 2034 2064 2301 2637 2811 3114 3858 3876
Size: 1204 30 237 336 174 303 744 18
Cuts at: 3876 4239 4493 830
Size: 363 254 3478
Fragments arranged by size:
3478 1204 744 363 336 303 254 237
174 30 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2857 3923 2857
Size: 1066 6075
SacII CC_GC'GG
Cuts at: 2042 2042
Size: 7141
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SanDI GG'GwC_CC
Cuts at: 2889 2889
Size: 7141
SapI GCTCTTCn'nnn_
Cuts at: 2681 2681
Size: 7141
SbfI CC_TGCA'GG
Cuts at: 2301 4239 4493 2301
Size: 1938 254 4949
Fragments arranged by size:
4949 1938 254
ScaI AGT'ACT
Cuts at: 5389 7101 5389
Size: 1712 5429
SexAI A'CCwGG_T
Cuts at: 3003 3003
Size: 7141
SfcI C'TryA_G
Cuts at: 826 1045 2030 2060 2297 2633 2807 3110 3671
Size: 219 985 30 237 336 174 303 561
Cuts at: 3671 3854 3872 4235 4489 5624 6305 6496 826
Size: 183 18 363 254 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 681 561 363 336 303
254 237 219 191 183 174 30 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3997 3997
Size: 7141
SfoI GGC'GCC
Cuts at: 3019 3394 3019
Size: 375 6766
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 2954 4468 1872
Size: 1082 1514 4545
Fragments arranged by size:
4545 1514 1082
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5245 6146 6408 820
Size: 197 926 3302 901 262 1553
Fragments arranged by size:
3302 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4932 493
Size: 4439 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 4499 1132
Size: 3367 3774
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 3651 1517
Size: 2134 5007
StyI C'CwwG_G
Cuts at: 513 1065 2004 3198 3226 3378 3405 4264 4580
Size: 552 939 1194 28 152 27 859 316
Cuts at: 4580 513
Size: 3074
Fragments arranged by size:
3074 1194 939 859 552 316 152 28
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3106 3144 3728 5168 5185 5338 5523
Size: 1255 1526 38 584 1440 17 153 185
Cuts at: 5523 5968 325
Size: 445 1498
Fragments arranged by size:
1526 1498 1440 1255 584 445 185 153
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5387 7099 96
Size: 274 80 33 51 1443 3410 1712 138
Fragments arranged by size:
3410 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 3232 3264 3739 3771 7101 7133 3053
Size: 32 147 32 475 32 3330 32 3061
Fragments arranged by size:
3330 3061 475 147 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 2491 1316
Size: 795 380 5966
Fragments arranged by size:
5966 795 380
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 3011 3385 3820 4183 4222 2323
Size: 688 374 435 363 39 5242
Fragments arranged by size:
5242 688 435 374 363 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 2952 4466 1870
Size: 1082 1514 4545
Fragments arranged by size:
4545 1514 1082
XmnI GAAnn'nnTTC
Cuts at: 3896 5270 3896
Size: 1374 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2092 2593 4949 276
Size: 53 83 186 936 558 501 2356 2468
Fragments arranged by size:
2468 2356 936 558 501 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SrfI
SwaI