Restriction map for
pFN21AB5763
(Circular) (Six-base) MAPSORT of: pFN21AB5763.seq Check: 1467 from: 1 to: 7153
pFN21AB5763 7153 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 7153
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4963 278
Size: 53 83 186 936 3427 2468
Fragments arranged by size:
3427 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 2720 4496 1937
Size: 466 317 1776 4594
Fragments arranged by size:
4594 1776 466 317
Acc65I G'GTAC_C
Cuts at: 2682 2840 4474 2682
Size: 158 1634 5361
Fragments arranged by size:
5361 1634 158
AclI AA'CG_TT
Cuts at: 1563 5280 5653 1563
Size: 3717 373 3063
Fragments arranged by size:
3717 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2333 2534 2573 5216 6600 6868 1632
Size: 407 294 201 39 2643 1384 268 1917
Fragments arranged by size:
2643 1917 1384 407 294 268 201 39
AfeI AGC'GCT
Cuts at: 3004 3004
Size: 7153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6956
AflIII A'CryG_T
Cuts at: 1203 2476 6052 7029 1203
Size: 1273 3576 977 1327
Fragments arranged by size:
3576 1327 1273 977
AgeI A'CCGG_T
Cuts at: 5984 5984
Size: 7153
AhdI GACnn_n'nnGTC
Cuts at: 2740 5882 2740
Size: 3142 4011
AleI CACnn'nnGTG
Cuts at: 1166 3758 1166
Size: 2592 4561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7153
AlwNI CAG_nnn'CTG
Cuts at: 3020 3023 3270 4896 6468 3020
Size: 3 247 1626 1572 3705
Fragments arranged by size:
3705 1626 1572 247 3
ApaI G_GGCC'C
Cuts at: 2064 3656 3948 2064
Size: 1592 292 5269
Fragments arranged by size:
5269 1592 292
ApaLI G'TGCA_C
Cuts at: 2626 5210 6366 2626
Size: 2584 1156 3413
Fragments arranged by size:
3413 2584 1156
ApoI r'AATT_y
Cuts at: 1493 2390 4250 4462 4688 4840 6949 1493
Size: 897 1860 212 226 152 2109 1697
Fragments arranged by size:
2109 1860 1697 897 226 212 152
AseI AT'TA_AT
Cuts at: 160 1032 5707 160
Size: 872 4675 1606
Fragments arranged by size:
4675 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7153
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2693 3123 4478 1870
Size: 73 114 636 430 1355 4545
Fragments arranged by size:
4545 1355 636 430 114 73
BamHI G'GATC_C
Cuts at: 4483 4850 4483
Size: 367 6786
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2682 2778 2840 2948 3410
Size: 325 217 627 895 96 62 108 462
Cuts at: 3410 4474 5929 618
Size: 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 895 627 462 325 217
108 96 62
BanII G_rGCy'C
Cuts at: 1391 2055 2064 2592 2700 3000 3277 3656 3872
Size: 664 9 528 108 300 277 379 216
Cuts at: 3872 3948 4102 4472 1391
Size: 76 154 370 4072
Fragments arranged by size:
4072 664 528 379 370 300 277 216
154 108 76 9
BbeI G_GCGC'C
Cuts at: 2782 2782
Size: 7153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 4101 928
Size: 971 135 2067 3980
Fragments arranged by size:
3980 2067 971 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5344 5378 1903
Size: 34 3407 34 3678
Fragments arranged by size:
3678 3407 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5045 6255 5045
Size: 1210 5943
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7153
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2059 2235 3149
Size: 107 122 71 631 605 387 176 914
Cuts at: 3149 5764 136
Size: 2615 1525
Fragments arranged by size:
2615 1525 914 631 605 387 176 122
107 71
BglII A'GATC_T
Cuts at: 7148 7148
Size: 7153
BlpI GC'TnA_GC
Cuts at: 4570 4570
Size: 7153
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2382 2630 2928 3085 3656 3948 5214
Size: 274 318 248 298 157 571 292 1266
Cuts at: 5214 6370 1790
Size: 1156 2573
Fragments arranged by size:
2573 1266 1156 571 318 298 292 274
248 157
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 6316
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 3273 3794
Size: 416 53 83 399 24 1841 15 521
Cuts at: 3794 3997 4884 5842 442
Size: 203 887 958 1753
Fragments arranged by size:
1841 1753 958 887 521 416 399 203
83 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 7153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3521 4021 4265 4427 5813 1239
Size: 2282 500 244 162 1386 2579
Fragments arranged by size:
2579 2282 1386 500 244 162
Bpu10I CC'TnA_GC
Cuts at: 2532 3510 2532
Size: 978 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2576 3440 5278 6143 6441 2306
Size: 270 864 1838 865 298 3018
Fragments arranged by size:
3018 1838 865 864 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 5816 882
Size: 617 675 3642 2219
Fragments arranged by size:
3642 2219 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 2479 4944 493
Size: 711 1203 72 2465 2702
Fragments arranged by size:
2702 2465 1203 711 72
BsaBI GATnn'nnATC
Cuts at: 7147 7147
Size: 7153
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2779 4089 4960 5342
Size: 53 83 186 936 1246 1310 871 382
Cuts at: 5342 5977 275
Size: 635 1451
Fragments arranged by size:
1451 1310 1246 936 871 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5586 5984 6258 6405 6816 5586
Size: 398 274 147 411 5923
Fragments arranged by size:
5923 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2924 2954 3802 3832 3922
Size: 30 165 30 1521 30 848 30 90
Cuts at: 3922 3952 1178
Size: 30 4379
Fragments arranged by size:
4379 1521 848 165 90 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 3481 3572 3810 1186
Size: 1257 1038 91 238 4529
Fragments arranged by size:
4529 1257 1038 238 91
BseYI C'CCAG_C
Cuts at: 2836 2974 2999 3488 3638 3745 3775 3837 3891
Size: 138 25 489 150 107 30 62 54
Cuts at: 3891 4146 6356 6852 2836
Size: 255 2210 496 3137
Fragments arranged by size:
3137 2210 496 489 255 150 138 107
62 54 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 3321 3903 1163
Size: 573 684 901 582 4413
Fragments arranged by size:
4413 901 684 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 4603 5364 6392 1540
Size: 275 186 955 1647 761 1028 2301
Fragments arranged by size:
2301 1647 1028 955 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 2630 2700 3687 5214 5299 6370 2592
Size: 38 70 987 1527 85 1071 3375
Fragments arranged by size:
3375 1527 1071 987 85 70 38
BsmI GAATG_Cn'
Cuts at: 4664 4757 4664
Size: 93 7060
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2064 2382 2592 2630 2700 2928
Size: 399 265 9 318 210 38 70 228
Cuts at: 2928 3000 3085 3277 3656 3687 3872 3948 4102
Size: 72 85 192 379 31 185 76 154
Cuts at: 4102 4472 5214 5299 6370 1391
Size: 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 370 318 265
228 210 192 185 154 85 85 76
72 70 38 31 9
BspEI T'CCGG_A
Cuts at: 6816 6816
Size: 7153
BspHI T'CATG_A
Cuts at: 2655 2826 5042 6772 2655
Size: 171 2216 1730 3036
Fragments arranged by size:
3036 2216 1730 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2442 4440 4508 844
Size: 1389 209 1998 68 3489
Fragments arranged by size:
3489 1998 1389 209 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2133 3553 3595 3616 5040 1394
Size: 429 310 1420 42 21 1424 3507
Fragments arranged by size:
3507 1424 1420 429 310 42 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 5648 5822 65
Size: 1183 4400 174 1396
Fragments arranged by size:
4400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2234 3471 3523 3763 5797 5984 1700
Size: 110 424 1237 52 240 2034 187 2869
Fragments arranged by size:
2869 2034 1237 424 240 187 110 52
BsrGI T'GTAC_A
Cuts at: 96 2806 96
Size: 2710 4443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7153
BssSI C'ACGA_G
Cuts at: 2616 5213 6225 2616
Size: 2597 1012 3544
Fragments arranged by size:
3544 2597 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3023 794
Size: 2229 4924
BstBI TT'CG_AA
Cuts at: 5957 5957
Size: 7153
BstEII G'GTnAC_C
Cuts at: 1184 2281 1184
Size: 1097 6056
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2569 3845 3932 3994 2113
Size: 456 1276 87 62 5272
Fragments arranged by size:
5272 1276 456 87 62
BstYI r'GATC_y
Cuts at: 1972 2259 2316 3198 4398 4483 4850 5235 5252
Size: 287 57 882 1200 85 367 385 17
Cuts at: 5252 6704 6790 6802 7148 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1200 882 385 367 346 287
86 85 57 17 12
BstZ17I GTA'TAC
Cuts at: 2721 2721
Size: 7153
Bsu36I CC'TnA_GG
Cuts at: 2303 2366 2303
Size: 63 7090
BtgI C'CryG_G
Cuts at: 513 1065 2004 3294 4357 513
Size: 552 939 1290 1063 3309
Fragments arranged by size:
3309 1290 1063 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 487
Size: 683 56 1106 5308
Fragments arranged by size:
5308 1106 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 4219 4669 5463 5483 2969
Size: 1250 450 794 20 4639
Fragments arranged by size:
4639 1250 794 450 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7118
DraI TTT'AAA
Cuts at: 2332 4457 2332
Size: 2125 5028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6160 809
Size: 507 4844 1802
Fragments arranged by size:
4844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2103 2236
Size: 54 1425 50 275 18 177 96 133
Cuts at: 2236 2247 2670 3045 4300 4600 5489 7118 8
Size: 11 423 375 1255 300 889 1629 43
Fragments arranged by size:
1629 1425 1255 889 423 375 300 275
177 133 96 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 4600 1537
Size: 275 2788 4090
Fragments arranged by size:
4090 2788 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 5083 1353
Size: 1439 2291 3423
Fragments arranged by size:
3423 2291 1439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2206 3179 3583 5740 6114 6260
Size: 1064 138 617 973 404 2157 374 146
Cuts at: 6260 387
Size: 1280
Fragments arranged by size:
2157 1280 1064 973 617 404 374 146
138
EcoICRI GAG'CTC
Cuts at: 2590 2698 2590
Size: 108 7045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2333 2534 2573 3521 4021 4265
Size: 393 407 294 201 39 948 500 244
Cuts at: 4265 4427 5216 5813 6600 6868 1239
Size: 162 789 597 787 268 1524
Fragments arranged by size:
1524 948 789 787 597 500 407 393
294 268 244 201 162 39
EcoO109I rG'GnC_Cy
Cuts at: 2196 2509 2962 3642 3652 3653 4174 4447 2196
Size: 313 453 680 10 1 521 273 4902
Fragments arranged by size:
4902 680 521 453 313 273 10 1
EcoRI G'AATT_C
Cuts at: 2390 4250 4462 4840 2390
Size: 1860 212 378 4703
Fragments arranged by size:
4703 1860 378 212
FspI TGC'GCA
Cuts at: 5659 5659
Size: 7153
HaeII r_GCGC'y
Cuts at: 1678 2782 3006 3544 3782 6300 1678
Size: 1104 224 538 238 2518 2531
Fragments arranged by size:
2531 2518 1104 538 238 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2726 2758 3669 3701 4301
Size: 32 135 32 1154 32 911 32 600
Cuts at: 4301 4333 4367 4399 5794 5826 5868 5900 1373
Size: 32 34 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1395 1154 911 600 135 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4135 4497 4743 669
Size: 988 281 2197 362 246 3079
Fragments arranged by size:
3079 2197 988 362 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7153
HpaI GTT'AAC
Cuts at: 4743 4743
Size: 7153
KasI G'GCGC_C
Cuts at: 2778 2778
Size: 7153
KpnI G_GTAC'C
Cuts at: 2686 2844 4478 2686
Size: 158 1634 5361
Fragments arranged by size:
5361 1634 158
MfeI C'AATT_G
Cuts at: 4752 4752
Size: 7153
MluI A'CGCG_T
Cuts at: 7029 7029
Size: 7153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 3690 3829 6267 6451 1305
Size: 1281 1104 139 2438 184 2007
Fragments arranged by size:
2438 2007 1281 1104 184 139
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 2672 3047 4302 10
Size: 54 1425 343 417 423 375 1255 2861
Fragments arranged by size:
2861 1425 1255 423 417 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 2831 3758 4277 5111 5470 5629
Size: 648 1662 3 927 519 834 359 159
Cuts at: 5629 518
Size: 2042
Fragments arranged by size:
2042 1662 927 834 648 519 359 159
3
MspA1I CmG'CkG
Cuts at: 1606 2090 2639 3020 3547 4569 5246 6394 6639
Size: 484 549 381 527 1022 677 1148 245
Cuts at: 6639 1606
Size: 2120
Fragments arranged by size:
2120 1148 1022 677 549 527 484 381
245
NaeI GCC'GGC
Cuts at: 3473 3765 3473
Size: 292 6861
NarI GG'CG_CC
Cuts at: 2779 2779
Size: 7153
NcoI C'CATG_G
Cuts at: 513 1065 2004 3294 4357 513
Size: 552 939 1290 1063 3309
Fragments arranged by size:
3309 1290 1063 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7153
NgoMIV G'CCGG_C
Cuts at: 3471 3763 3471
Size: 292 6861
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7153
NotI GC'GGCC_GC
Cuts at: 4600 4600
Size: 7153
NspI r_CATG'y
Cuts at: 1132 1229 2524 2986 4511 6056 1132
Size: 97 1295 462 1525 1545 2229
Fragments arranged by size:
2229 1545 1525 1295 462 97
PasI CC'CwG_GG
Cuts at: 1348 2928 3270 4022 1348
Size: 1580 342 752 4479
Fragments arranged by size:
4479 1580 752 342
PciI A'CATG_T
Cuts at: 6052 6052
Size: 7153
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 2679 3270 3911 4022 4298 1108
Size: 1137 434 591 641 111 276 3963
Fragments arranged by size:
3963 1137 641 591 434 276 111
PfoI T'CCnGG_A
Cuts at: 2412 4443 2412
Size: 2031 5122
PmeI GTTT'AAAC
Cuts at: 4457 4457
Size: 7153
PmlI CAC'GTG
Cuts at: 2479 2479
Size: 7153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5222 5254 6735 6767 5222
Size: 32 1481 32 5608
Fragments arranged by size:
5608 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4174 4447 4174
Size: 273 6880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7153
PsiI TTA'TAA
Cuts at: 4723 4723
Size: 7153
PspOMI G'GGCC_C
Cuts at: 2060 3652 3944 2060
Size: 1592 292 5269
Fragments arranged by size:
5269 1592 292
PstI C_TGCA'G
Cuts at: 830 3366 4392 4505 830
Size: 2536 1026 113 3478
Fragments arranged by size:
3478 2536 1026 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7153
PvuII CAG'CTG
Cuts at: 3020 3020
Size: 7153
SacI G_AGCT'C
Cuts at: 2592 2700 2592
Size: 108 7045
SalI G'TCGA_C
Cuts at: 1936 4495 1936
Size: 2559 4594
SapI GCTCTTCn'nnn_
Cuts at: 2792 2792
Size: 7153
SbfI CC_TGCA'GG
Cuts at: 4505 4505
Size: 7153
ScaI AGT'ACT
Cuts at: 2651 5401 7113 2651
Size: 2750 1712 2691
Fragments arranged by size:
2750 2691 1712
SfcI C'TryA_G
Cuts at: 826 1045 2097 2816 3218 3362 3831 4388 4501
Size: 219 1052 719 402 144 469 557 113
Cuts at: 4501 5636 6317 6508 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1052 719 681 557 469 402
219 191 144 113
SfoI GGC'GCC
Cuts at: 2780 2780
Size: 7153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7153
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7153
SmaI CCC'GGG
Cuts at: 1872 2059 4480 1872
Size: 187 2421 4545
Fragments arranged by size:
4545 2421 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 2591 3455 5257 6158 6420
Size: 197 926 378 270 864 1802 901 262
Cuts at: 6420 820
Size: 1553
Fragments arranged by size:
1802 1553 926 901 864 378 270 262
197
SnaBI TAC'GTA
Cuts at: 493 4944 493
Size: 4451 2702
SpeI A'CTAG_T
Cuts at: 152 3679 152
Size: 3527 3626
SphI G_CATG'C
Cuts at: 1132 2986 4511 1132
Size: 1854 1525 3774
Fragments arranged by size:
3774 1854 1525
SspI AAT'ATT
Cuts at: 5 52 5077 5
Size: 47 5025 2081
Fragments arranged by size:
5025 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7153
StyI C'CwwG_G
Cuts at: 513 1065 2004 3294 3588 3669 4357 4592 513
Size: 552 939 1290 294 81 688 235 3074
Fragments arranged by size:
3074 1290 939 688 552 294 235 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5180 5197 5350 5535 5980 325
Size: 1255 3600 17 153 185 445 1498
Fragments arranged by size:
3600 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 2649 2806
Size: 274 80 33 51 1443 540 132 157
Cuts at: 2806 5399 7111 96
Size: 2593 1712 138
Fragments arranged by size:
2593 1712 1443 540 274 157 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3922 3954 7113 7145 3922
Size: 32 3159 32 3930
Fragments arranged by size:
3930 3159 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7153
XbaI T'CTAG_A
Cuts at: 4489 4489
Size: 7153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3496 3667 3991 3496
Size: 171 324 6658
Fragments arranged by size:
6658 324 171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7153
XmaI C'CCGG_G
Cuts at: 1870 2057 4478 1870
Size: 187 2421 4545
Fragments arranged by size:
4545 2421 187
XmnI GAAnn'nnTTC
Cuts at: 5282 5282
Size: 7153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4961 276
Size: 53 83 186 936 3427 2468
Fragments arranged by size:
3427 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI