Restriction map for
pFN21AB5757
(Circular) (Six-base) MAPSORT of: pFN21AB5757.seq Check: 4294 from: 1 to: 7147
pFN21AB5757 7147 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2686 2686
Size: 7147
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4957 278
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4490 1937
Size: 2553 4594
Acc65I G'GTAC_C
Cuts at: 4468 4468
Size: 7147
AclI AA'CG_TT
Cuts at: 1563 5274 5647 1563
Size: 3711 373 3063
Fragments arranged by size:
3711 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2341 2593 5210 6594 6862 1632
Size: 709 252 2617 1384 268 1917
Fragments arranged by size:
2617 1917 1384 709 268 252
AfeI AGC'GCT
Cuts at: 2568 3786 4035 2568
Size: 1218 249 5680
Fragments arranged by size:
5680 1218 249
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6950
AflIII A'CryG_T
Cuts at: 1203 2706 4338 6046 7023 1203
Size: 1503 1632 1708 977 1327
Fragments arranged by size:
1708 1632 1503 1327 977
AgeI A'CCGG_T
Cuts at: 5978 5978
Size: 7147
AhdI GACnn_n'nnGTC
Cuts at: 3650 5876 3650
Size: 2226 4921
AleI CACnn'nnGTG
Cuts at: 1166 2513 3182 1166
Size: 1347 669 5131
Fragments arranged by size:
5131 1347 669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3072 3935 1184
Size: 1888 863 4396
Fragments arranged by size:
4396 1888 863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 7115
AlwNI CAG_nnn'CTG
Cuts at: 2227 2732 3287 3727 4308 4890 6462 2227
Size: 505 555 440 581 582 1572 2912
Fragments arranged by size:
2912 1572 582 581 555 505 440
ApaI G_GGCC'C
Cuts at: 3550 4317 3550
Size: 767 6380
ApaLI G'TGCA_C
Cuts at: 5204 6360 5204
Size: 1156 5991
ApoI r'AATT_y
Cuts at: 1493 4456 4682 4834 6943 1493
Size: 2963 226 152 2109 1697
Fragments arranged by size:
2963 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5701 160
Size: 872 4669 1606
Fragments arranged by size:
4669 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2277 2549 2600 2777 3201 3694
Size: 73 87 247 272 51 177 424 493
Cuts at: 3694 3906 4107 4472 1870
Size: 212 201 365 4545
Fragments arranged by size:
4545 493 424 365 272 247 212 201
177 87 73 51
BamHI G'GATC_C
Cuts at: 2390 3294 4477 4844 2390
Size: 904 1183 367 4693
Fragments arranged by size:
4693 1183 904 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2113 2507 3166 3404
Size: 325 217 627 319 7 394 659 238
Cuts at: 3404 3515 4347 4468 5923 618
Size: 111 832 121 1455 1842
Fragments arranged by size:
1842 1455 832 659 627 394 325 319
238 217 121 111 7
BanII G_rGCy'C
Cuts at: 1391 2277 2607 2625 3120 3550 3572 3588 4256
Size: 886 330 18 495 430 22 16 668
Cuts at: 4256 4317 4466 1391
Size: 61 149 4072
Fragments arranged by size:
4072 886 668 495 430 330 149 61
22 18 16
BbeI G_GCGC'C
Cuts at: 2110 2117 3519 2110
Size: 7 1402 5738
Fragments arranged by size:
5738 1402 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2278 2308 2351 3267 928
Size: 971 379 30 43 916 4808
Fragments arranged by size:
4808 971 916 379 43 30
BbvCI CC'TCA_GC
Cuts at: 2737 3605 3674 3809 2737
Size: 868 69 135 6075
Fragments arranged by size:
6075 868 135 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5338 5372 1903
Size: 34 3401 34 3678
Fragments arranged by size:
3678 3401 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2176 5039 6249 2176
Size: 2863 1210 3074
Fragments arranged by size:
3074 2863 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7147
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 3065 3750
Size: 107 122 71 631 605 1144 249 685
Cuts at: 3750 4331 5758 136
Size: 581 1427 1525
Fragments arranged by size:
1525 1427 1144 685 631 605 581 249
122 107 71
BglII A'GATC_T
Cuts at: 7142 7142
Size: 7147
BlpI GC'TnA_GC
Cuts at: 3069 3233 3314 3566 4037 4250 4564 3069
Size: 164 81 252 471 213 314 5652
Fragments arranged by size:
5652 471 314 252 213 164 81
Bme1580I G_kGCm'C
Cuts at: 1790 3169 3550 3803 4317 4350 4417 5208 6364
Size: 1379 381 253 514 33 67 791 1156
Cuts at: 6364 1790
Size: 2573
Fragments arranged by size:
2573 1379 1156 791 514 381 253 67
33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2893 4176 4318
Size: 416 53 83 399 24 1476 1283 142
Cuts at: 4318 4878 5836 442
Size: 560 958 1753
Fragments arranged by size:
1753 1476 1283 958 560 416 399 142
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 4062 2738
Size: 1324 5823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2438 2510 2733 3446 3619 5807 1239
Size: 1181 18 72 223 713 173 2188 2579
Fragments arranged by size:
2579 2188 1181 713 223 173 72 18
Bpu10I CC'TnA_GC
Cuts at: 2123 2737 3605 3674 3809 2123
Size: 614 868 69 135 5461
Fragments arranged by size:
5461 868 614 135 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3320 5272 6137 6435 3320
Size: 1952 865 298 4032
Fragments arranged by size:
4032 1952 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5810 882
Size: 617 4311 2219
Fragments arranged by size:
4311 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 4938 493
Size: 711 2106 1628 2702
Fragments arranged by size:
2702 2106 1628 711
BsaBI GATnn'nnATC
Cuts at: 7141 7141
Size: 7147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2107 2114 2283
Size: 53 83 186 936 477 97 7 169
Cuts at: 2283 2842 3323 3378 3516 3762 3999 4954 5336
Size: 559 481 55 138 246 237 955 382
Cuts at: 5336 5971 275
Size: 635 1451
Fragments arranged by size:
1451 955 936 635 559 481 477 382
246 237 186 169 138 97 83 55
53 7
BsaWI w'CCGG_w
Cuts at: 5580 5978 6252 6399 6810 5580
Size: 398 274 147 411 5917
Fragments arranged by size:
5917 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2820 2850 1178
Size: 30 165 30 1417 30 5475
Fragments arranged by size:
5475 1417 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2095 2285 3497 4001 1186
Size: 826 83 190 1212 504 4332
Fragments arranged by size:
4332 1212 826 504 190 83
BseYI C'CCAG_C
Cuts at: 2227 3303 3384 3708 4098 6350 6846 2227
Size: 1076 81 324 390 2252 496 2528
Fragments arranged by size:
2528 2252 1076 496 390 324 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2517 3050 3122 3499 3650 3908 4076
Size: 573 781 533 72 377 151 258 168
Cuts at: 4076 1163
Size: 4234
Fragments arranged by size:
4234 781 573 533 377 258 168 151
72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 3641 4597 5358 6386 1540
Size: 275 186 1591 49 956 761 1028 2301
Fragments arranged by size:
2301 1591 1028 956 761 275 186 49
BsiHKAI G_wGCw'C
Cuts at: 2607 2625 3315 3588 4256 5208 5293 6364 2607
Size: 18 690 273 668 952 85 1071 3390
Fragments arranged by size:
3390 1071 952 690 668 273 85 18
BsmI GAATG_Cn'
Cuts at: 4658 4751 4658
Size: 93 7054
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2546 2709 2189
Size: 357 163 6627
Fragments arranged by size:
6627 357 163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2607 2625 3120 3169 3315 3550
Size: 399 487 330 18 495 49 146 235
Cuts at: 3550 3572 3588 3803 4256 4317 4350 4417 4466
Size: 22 16 215 453 61 33 67 49
Cuts at: 4466 5208 5293 6364 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 487 453 399 330
235 215 146 85 67 61 49 49
33 22 18 16
BspEI T'CCGG_A
Cuts at: 6810 6810
Size: 7147
BspHI T'CATG_A
Cuts at: 5036 6766 5036
Size: 1730 5417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2686 2874 3540 4502 844
Size: 1842 188 666 962 3489
Fragments arranged by size:
3489 1842 962 666 188
BsrBI CCG'CTC
Cuts at: 1394 1823 3073 5034 1394
Size: 429 1250 1961 3507
Fragments arranged by size:
3507 1961 1250 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5642 5816 65
Size: 1183 4394 174 1396
Fragments arranged by size:
4394 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 3401 5791 5978 1700
Size: 110 638 953 2390 187 2869
Fragments arranged by size:
2869 2390 953 638 187 110
BsrGI T'GTAC_A
Cuts at: 96 2679 96
Size: 2583 4564
BssHII G'CGCG_C
Cuts at: 1925 3629 1925
Size: 1704 5443
BssSI C'ACGA_G
Cuts at: 3872 5207 6219 3872
Size: 1335 1012 4800
Fragments arranged by size:
4800 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3477 794
Size: 2683 4464
BstBI TT'CG_AA
Cuts at: 5951 5951
Size: 7147
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7147
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 3364 4129 2416
Size: 948 765 5434
Fragments arranged by size:
5434 948 765
BstYI r'GATC_y
Cuts at: 1972 2390 2634 3294 3420 4477 4844 5229 5246
Size: 418 244 660 126 1057 367 385 17
Cuts at: 5246 6698 6784 6796 7142 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1057 660 418 385 367 346
244 126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3062 3062
Size: 7147
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 3519 3541 513
Size: 552 939 405 1110 22 4119
Fragments arranged by size:
4119 1110 939 552 405 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4120 4290 487
Size: 683 56 2894 170 3344
Fragments arranged by size:
3344 2894 683 170 56
BtsI GCAGTG_nn'
Cuts at: 2730 4415 4663 5457 5477 2730
Size: 1685 248 794 20 4400
Fragments arranged by size:
4400 1685 794 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2868 2903 529
Size: 35 2304 35 4773
Fragments arranged by size:
4773 2304 35 35
DraI TTT'AAA
Cuts at: 4451 4451
Size: 7147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6154 809
Size: 507 4838 1802
Fragments arranged by size:
4838 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2450 2479 2889
Size: 54 1425 50 275 18 620 29 410
Cuts at: 2889 3186 3289 3589 3638 3837 4594 5483 7112
Size: 297 103 300 49 199 757 889 1629
Cuts at: 7112 8
Size: 43
Fragments arranged by size:
1629 1425 889 757 620 410 300 297
275 199 103 54 50 49 43 29
18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 3638 4594 1537
Size: 275 1777 49 956 4090
Fragments arranged by size:
4090 1777 956 275 49
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 5077 1353
Size: 1259 2465 3423
Fragments arranged by size:
3423 2465 1259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2717 5734 6108 6254 387
Size: 1064 138 1128 3017 374 146 1280
Fragments arranged by size:
3017 1280 1128 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2623 3586 4254 2605
Size: 18 963 668 5498
Fragments arranged by size:
5498 963 668 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2341 2420 2438 2510 2593 2733 3446
Size: 393 709 79 18 72 83 140 713
Cuts at: 3446 3619 5210 5807 6594 6862 1239
Size: 173 1591 597 787 268 1524
Fragments arranged by size:
1591 1524 787 713 709 597 393 268
173 140 83 79 72 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2588 3158 3368 3608 2465
Size: 123 570 210 240 6004
Fragments arranged by size:
6004 570 240 210 123
EcoO109I rG'GnC_Cy
Cuts at: 2139 2255 2404 2863 2983 3040 3205 3529 3546
Size: 116 149 459 120 57 165 324 17
Cuts at: 3546 3547 3652 4313 4314 2139
Size: 1 105 661 1 4972
Fragments arranged by size:
4972 661 459 324 165 149 120 116
105 57 17 1 1
EcoRI G'AATT_C
Cuts at: 4456 4834 4456
Size: 378 6769
FspI TGC'GCA
Cuts at: 5653 5653
Size: 7147
HaeII r_GCGC'y
Cuts at: 1678 2110 2117 2570 3519 3788 3846 4037 6294
Size: 432 7 453 949 269 58 191 2257
Cuts at: 6294 1678
Size: 2531
Fragments arranged by size:
2531 2257 949 453 432 269 191 58
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3345 3365 3377 3397 3636
Size: 32 135 32 1773 20 12 20 239
Cuts at: 3636 3668 3939 3971 5788 5820 5862 5894 1373
Size: 32 271 32 1817 32 42 32 2626
Fragments arranged by size:
2626 1817 1773 271 239 135 42 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3616 4491 4737 669
Size: 988 281 1678 875 246 3079
Fragments arranged by size:
3079 1678 988 875 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7147
HpaI GTT'AAC
Cuts at: 4737 4737
Size: 7147
KasI G'GCGC_C
Cuts at: 2106 2113 3515 2106
Size: 7 1402 5738
Fragments arranged by size:
5738 1402 7
KpnI G_GTAC'C
Cuts at: 4472 4472
Size: 7147
MfeI C'AATT_G
Cuts at: 4746 4746
Size: 7147
MluI A'CGCG_T
Cuts at: 7023 7023
Size: 7147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 4341 6261 6445 1305
Size: 888 2148 1920 184 2007
Fragments arranged by size:
2148 2007 1920 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2891 3188 3839 10
Size: 54 1425 343 1059 297 651 3318
Fragments arranged by size:
3318 1425 1059 651 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3182 5105 5464 5623 518
Size: 648 1347 669 1923 359 159 2042
Fragments arranged by size:
2042 1923 1347 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2826 3046 3223 3565 3747 4018 4563
Size: 621 599 220 177 342 182 271 545
Cuts at: 4563 5240 6388 6633 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 599 545 342 271
245 220 182 177
NarI GG'CG_CC
Cuts at: 2107 2114 3516 2107
Size: 7 1402 5738
Fragments arranged by size:
5738 1402 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5656
Fragments arranged by size:
5656 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7147
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7147
NotI GC'GGCC_GC
Cuts at: 3638 4594 3638
Size: 956 6191
NspI r_CATG'y
Cuts at: 1132 1229 2710 3101 4342 4505 6050 1132
Size: 97 1481 391 1241 163 1545 2229
Fragments arranged by size:
2229 1545 1481 1241 391 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7147
PciI A'CATG_T
Cuts at: 2706 4338 6046 2706
Size: 1632 1708 3807
Fragments arranged by size:
3807 1708 1632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7147
PfoI T'CCnGG_A
Cuts at: 2071 2904 3129 3423 3687 2071
Size: 833 225 294 264 5531
Fragments arranged by size:
5531 833 294 264 225
PmeI GTTT'AAAC
Cuts at: 4451 4451
Size: 7147
PmlI CAC'GTG
Cuts at: 3310 3310
Size: 7147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5216 5248 6729 6761 5216
Size: 32 1481 32 5602
Fragments arranged by size:
5602 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2255 2404 2863 3205 3529 3652 2255
Size: 149 459 342 324 123 5750
Fragments arranged by size:
5750 459 342 324 149 123
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7147
PsiI TTA'TAA
Cuts at: 4717 4717
Size: 7147
PspOMI G'GGCC_C
Cuts at: 3546 4313 3546
Size: 767 6380
PstI C_TGCA'G
Cuts at: 830 2466 2589 2697 3051 3228 4171 4499 830
Size: 1636 123 108 354 177 943 328 3478
Fragments arranged by size:
3478 1636 943 354 328 177 123 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7147
PvuII CAG'CTG
Cuts at: 2227 3223 3565 4018 2227
Size: 996 342 453 5356
Fragments arranged by size:
5356 996 453 342
SacI G_AGCT'C
Cuts at: 2607 2625 3588 4256 2607
Size: 18 963 668 5498
Fragments arranged by size:
5498 963 668 18
SalI G'TCGA_C
Cuts at: 1936 4489 1936
Size: 2553 4594
SanDI GG'GwC_CC
Cuts at: 2255 3205 2255
Size: 950 6197
SapI GCTCTTCn'nnn_
Cuts at: 2612 2612
Size: 7147
SbfI CC_TGCA'GG
Cuts at: 2466 2697 4499 2466
Size: 231 1802 5114
Fragments arranged by size:
5114 1802 231
ScaI AGT'ACT
Cuts at: 5395 7107 5395
Size: 1712 5435
SexAI A'CCwGG_T
Cuts at: 3648 3648
Size: 7147
SfcI C'TryA_G
Cuts at: 826 1045 2242 2462 2585 2693 3047 3224 4167
Size: 219 1197 220 123 108 354 177 943
Cuts at: 4167 4495 5630 6311 6502 826
Size: 328 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 943 681 354 328 220
219 191 177 123 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 2816 2816
Size: 7147
SfoI GGC'GCC
Cuts at: 2108 2115 3517 2108
Size: 7 1402 5738
Fragments arranged by size:
5738 1402 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7147
SmaI CCC'GGG
Cuts at: 1872 2279 3203 4474 1872
Size: 407 924 1271 4545
Fragments arranged by size:
4545 1271 924 407
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3335 5251 6152 6414 820
Size: 197 926 1392 1916 901 262 1553
Fragments arranged by size:
1916 1553 1392 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4938 493
Size: 4445 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7147
SphI G_CATG'C
Cuts at: 1132 4505 1132
Size: 3373 3774
SspI AAT'ATT
Cuts at: 5 52 5071 5
Size: 47 5019 2081
Fragments arranged by size:
5019 2081 47
StuI AGG'CCT
Cuts at: 1517 3504 3663 3807 1517
Size: 1987 159 144 4857
Fragments arranged by size:
4857 1987 159 144
StyI C'CwwG_G
Cuts at: 513 1065 2004 3084 3243 3493 4586 513
Size: 552 939 1080 159 250 1093 3074
Fragments arranged by size:
3074 1093 1080 939 552 250 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2875 5174 5191 5344 5529 5974 325
Size: 1255 1295 2299 17 153 185 445 1498
Fragments arranged by size:
2299 1498 1295 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 5393 7105
Size: 274 80 33 51 1443 702 2714 1712
Cuts at: 7105 96
Size: 138
Fragments arranged by size:
2714 1712 1443 702 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2820 2852 7107 7139 2247
Size: 32 541 32 4255 32 2255
Fragments arranged by size:
4255 2255 541 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3761 3953 1316
Size: 2445 192 4510
Fragments arranged by size:
4510 2445 192
XbaI T'CTAG_A
Cuts at: 4483 4483
Size: 7147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3421 4126 3421
Size: 705 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7147
XmaI C'CCGG_G
Cuts at: 1870 2277 3201 4472 1870
Size: 407 924 1271 4545
Fragments arranged by size:
4545 1271 924 407
XmnI GAAnn'nnTTC
Cuts at: 2315 5276 2315
Size: 2961 4186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4955 276
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SrfI SwaI