Restriction map for
pFN21AB5755
(Circular) (Six-base) MAPSORT of: pFN21AB5755.seq Check: 4074 from: 1 to: 7135
pFN21AB5755 7135 bp
With 182 enzymes: SgfI *
May 9, 2016 12:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2428 2192
Size: 236 6899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4945 278
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4478 1937
Size: 2541 4594
Acc65I G'GTAC_C
Cuts at: 4456 4456
Size: 7135
AclI AA'CG_TT
Cuts at: 1563 5262 5635 1563
Size: 3699 373 3063
Fragments arranged by size:
3699 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 2852 5198 6582 6850 1632
Size: 1079 141 2346 1384 268 1917
Fragments arranged by size:
2346 1917 1384 1079 268 141
AfeI AGC'GCT
Cuts at: 2762 2762
Size: 7135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6938
AflIII A'CryG_T
Cuts at: 1203 6034 7011 1203
Size: 4831 977 1327
Fragments arranged by size:
4831 1327 977
AgeI A'CCGG_T
Cuts at: 5966 5966
Size: 7135
AhdI GACnn_n'nnGTC
Cuts at: 5864 5864
Size: 7135
AleI CACnn'nnGTG
Cuts at: 1166 2901 3822 1166
Size: 1735 921 4479
Fragments arranged by size:
4479 1735 921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2919 1184
Size: 1735 5400
AlwNI CAG_nnn'CTG
Cuts at: 2830 4878 6450 2830
Size: 2048 1572 3515
Fragments arranged by size:
3515 2048 1572
ApaI G_GGCC'C
Cuts at: 2490 3001 2490
Size: 511 6624
ApaLI G'TGCA_C
Cuts at: 2904 3918 4356 5192 6348 2904
Size: 1014 438 836 1156 3691
Fragments arranged by size:
3691 1156 1014 836 438
ApoI r'AATT_y
Cuts at: 1493 2177 2592 2672 2956 4444 4670 4822 6931
Size: 684 415 80 284 1488 226 152 2109
Cuts at: 6931 1493
Size: 1697
Fragments arranged by size:
2109 1697 1488 684 415 284 226 152
80
AseI AT'TA_AT
Cuts at: 160 1032 5689 160
Size: 872 4657 1606
Fragments arranged by size:
4657 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
AvaI C'yCGr_G
Cuts at: 1870 1943 4460 1870
Size: 73 2517 4545
Fragments arranged by size:
4545 2517 73
BamHI G'GATC_C
Cuts at: 4465 4832 4465
Size: 367 6768
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3006 3228 3830 4456 5911
Size: 325 217 627 1219 222 602 626 1455
Cuts at: 5911 618
Size: 1842
Fragments arranged by size:
1842 1455 1219 627 626 602 325 222
217
BanII G_rGCy'C
Cuts at: 1391 2490 2645 3001 3534 3987 4454 1391
Size: 1099 155 356 533 453 467 4072
Fragments arranged by size:
4072 1099 533 467 453 356 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3242 3447 928
Size: 971 1343 205 4616
Fragments arranged by size:
4616 1343 971 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4220 4254 5326 5360 1903
Size: 34 2283 34 1072 34 3678
Fragments arranged by size:
3678 2283 1072 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2651 3085 3461 5027 6237 2651
Size: 434 376 1566 1210 3549
Fragments arranged by size:
3549 1566 1210 434 376
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7135
BfrBI ATG'CAT
Cuts at: 2563 2563
Size: 7135
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5746 136
Size: 107 122 71 631 605 4074 1525
Fragments arranged by size:
4074 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7130 7130
Size: 7135
BlpI GC'TnA_GC
Cuts at: 4016 4552 4016
Size: 536 6599
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2886 2908 3001 3922 4360 5196 6352
Size: 700 396 22 93 921 438 836 1156
Cuts at: 6352 1790
Size: 2573
Fragments arranged by size:
2573 1156 921 836 700 438 396 93
22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 3065 3507
Size: 416 53 83 399 24 911 737 442
Cuts at: 3507 3526 4866 5824 442
Size: 19 1340 958 1753
Fragments arranged by size:
1753 1340 958 911 737 442 416 399
83 53 24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2920 3540 5795 1239
Size: 1226 455 620 2255 2579
Fragments arranged by size:
2579 2255 1226 620 455
Bpu10I CC'TnA_GC
Cuts at: 3108 3367 3108
Size: 259 6876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 3699 4136 5260 6125 6423 2561
Size: 1138 437 1124 865 298 3273
Fragments arranged by size:
3273 1138 1124 865 437 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5798 882
Size: 617 4299 2219
Fragments arranged by size:
4299 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4926 493
Size: 711 3722 2702
Fragments arranged by size:
3722 2702 711
BsaBI GATnn'nnATC
Cuts at: 7129 7129
Size: 7135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3503 4942 5324 5959
Size: 53 83 186 936 1970 1439 382 635
Cuts at: 5959 275
Size: 1451
Fragments arranged by size:
1970 1451 1439 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4089 5568 5966 6240 6387 6798 4089
Size: 1479 398 274 147 411 4426
Fragments arranged by size:
4426 1479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2782 2812 1178
Size: 30 165 30 1379 30 5501
Fragments arranged by size:
5501 1379 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2462 3055 1186
Size: 994 282 593 5266
Fragments arranged by size:
5266 994 593 282
BseYI C'CCAG_C
Cuts at: 2125 2763 3329 4051 4113 4161 4241 6338 6834
Size: 638 566 722 62 48 80 2097 496
Cuts at: 6834 2125
Size: 2426
Fragments arranged by size:
2426 2097 722 638 566 496 80 62
48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2773 1163
Size: 573 436 601 5525
Fragments arranged by size:
5525 601 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4585 5346 6374 1540
Size: 275 186 2584 761 1028 2301
Fragments arranged by size:
2584 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2908 3922 4360 5196 5281 6352 2908
Size: 1014 438 836 85 1071 3691
Fragments arranged by size:
3691 1071 1014 836 438 85
BsmI GAATG_Cn'
Cuts at: 2192 2624 3819 4646 4739 2192
Size: 432 1195 827 93 4588
Fragments arranged by size:
4588 1195 827 432 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2645 2886 2908 3001 3534 3922
Size: 399 700 155 241 22 93 533 388
Cuts at: 3922 3987 4360 4454 5196 5281 6352 1391
Size: 65 373 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 700 533 399 388 373
241 155 94 93 85 65 22
BspEI T'CCGG_A
Cuts at: 6798 6798
Size: 7135
BspHI T'CATG_A
Cuts at: 5024 6754 5024
Size: 1730 5405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2428 4490 844
Size: 1348 236 2062 3489
Fragments arranged by size:
3489 2062 1348 236
BsrBI CCG'CTC
Cuts at: 1394 1823 5022 1394
Size: 429 3199 3507
Fragments arranged by size:
3507 3199 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 5630 5804 65
Size: 1183 1315 3067 174 1396
Fragments arranged by size:
3067 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3225 5779 5966 1700
Size: 110 1415 2554 187 2869
Fragments arranged by size:
2869 2554 1415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7135
BssSI C'ACGA_G
Cuts at: 5195 6207 5195
Size: 1012 6123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7135
BstBI TT'CG_AA
Cuts at: 5939 5939
Size: 7135
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7135
BstXI CCAn_nnnn'nTGG
Cuts at: 3146 3777 3146
Size: 631 6504
BstYI r'GATC_y
Cuts at: 1972 4465 4832 5217 5234 6686 6772 6784 7130
Size: 2493 367 385 17 1452 86 12 346
Cuts at: 7130 1972
Size: 1977
Fragments arranged by size:
2493 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3002 3078 3139 3569 513
Size: 552 939 998 76 61 430 4079
Fragments arranged by size:
4079 998 939 552 430 76 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6396
Fragments arranged by size:
6396 683 56
BtsI GCAGTG_nn'
Cuts at: 2575 3868 4651 5445 5465 2575
Size: 1293 783 794 20 4245
Fragments arranged by size:
4245 1293 794 783 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3014 3049 4059 4094 529
Size: 35 2450 35 1010 35 3570
Fragments arranged by size:
3570 2450 1010 35 35 35
DraI TTT'AAA
Cuts at: 4439 4439
Size: 7135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3332 6142 809
Size: 507 2016 2810 1802
Fragments arranged by size:
2810 2016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4251 4582 5471
Size: 54 1425 50 275 18 2421 331 889
Cuts at: 5471 7100 8
Size: 1629 43
Fragments arranged by size:
2421 1629 1425 889 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4582 1537
Size: 275 2770 4090
Fragments arranged by size:
4090 2770 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2544 4210 5065 1353
Size: 1191 1666 855 3423
Fragments arranged by size:
3423 1666 1191 855
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3387 5722 6096 6242 387
Size: 1064 138 1798 2335 374 146 1280
Fragments arranged by size:
2335 1798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2711 2852 2920 3540 5198 5795
Size: 393 833 246 141 68 620 1658 597
Cuts at: 5795 6582 6850 1239
Size: 787 268 1524
Fragments arranged by size:
1658 1524 833 787 620 597 393 268
246 141 68
EcoNI CCTnn'n_nnAGG
Cuts at: 4151 4151
Size: 7135
EcoO109I rG'GnC_Cy
Cuts at: 2486 2487 2997 2998 3305 4289 2486
Size: 1 510 1 307 984 5332
Fragments arranged by size:
5332 984 510 307 1 1
EcoRI G'AATT_C
Cuts at: 2177 4444 4822 2177
Size: 2267 378 4490
Fragments arranged by size:
4490 2267 378
EcoRV GAT'ATC
Cuts at: 3840 3840
Size: 7135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2042 3812 2042
Size: 1770 5365
FspI TGC'GCA
Cuts at: 5641 5641
Size: 7135
HaeII r_GCGC'y
Cuts at: 1678 2764 6282 1678
Size: 1086 3518 2531
Fragments arranged by size:
3518 2531 1086
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5776 5808 5850 5882 1373
Size: 32 135 32 4204 32 42 32 2626
Fragments arranged by size:
4204 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3858 4479 4725 669
Size: 988 281 1920 621 246 3079
Fragments arranged by size:
3079 1920 988 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7135
HpaI GTT'AAC
Cuts at: 4725 4725
Size: 7135
KpnI G_GTAC'C
Cuts at: 4460 4460
Size: 7135
MfeI C'AATT_G
Cuts at: 3086 4734 3086
Size: 1648 5487
MluI A'CGCG_T
Cuts at: 7011 7011
Size: 7135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6249 6433 1305
Size: 4944 184 2007
Fragments arranged by size:
4944 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5313
Fragments arranged by size:
5313 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2568 2652 2901 3144 3822 5093
Size: 648 1098 304 84 249 243 678 1271
Cuts at: 5093 5452 5611 518
Size: 359 159 2042
Fragments arranged by size:
2042 1271 1098 678 648 359 304 249
243 159 84
MspA1I CmG'CkG
Cuts at: 1606 2125 2787 4551 5228 6376 6621 1606
Size: 519 662 1764 677 1148 245 2120
Fragments arranged by size:
2120 1764 1148 677 662 519 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3002 3139 513
Size: 552 939 998 137 4509
Fragments arranged by size:
4509 998 939 552 137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7135
NotI GC'GGCC_GC
Cuts at: 4582 4582
Size: 7135
NsiI A_TGCA'T
Cuts at: 2565 2565
Size: 7135
NspI r_CATG'y
Cuts at: 1132 1229 3950 4493 6038 1132
Size: 97 2721 543 1545 2229
Fragments arranged by size:
2721 2229 1545 543 97
PasI CC'CwG_GG
Cuts at: 1348 2089 1348
Size: 741 6394
PciI A'CATG_T
Cuts at: 6034 6034
Size: 7135
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2810 3260 3874 1108
Size: 1702 450 614 4369
Fragments arranged by size:
4369 1702 614 450
PfoI T'CCnGG_A
Cuts at: 3126 3736 3126
Size: 610 6525
PmeI GTTT'AAAC
Cuts at: 4439 4439
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 6717 6749 5204
Size: 32 1481 32 5590
Fragments arranged by size:
5590 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4289 4289
Size: 7135
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7135
PsiI TTA'TAA
Cuts at: 4705 4705
Size: 7135
PspOMI G'GGCC_C
Cuts at: 2486 2997 2486
Size: 511 6624
PstI C_TGCA'G
Cuts at: 830 2189 2462 3284 4487 830
Size: 1359 273 822 1203 3478
Fragments arranged by size:
3478 1359 1203 822 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7135
PvuII CAG'CTG
Cuts at: 2125 2125
Size: 7135
RsrII CG'GwC_CG
Cuts at: 4092 4092
Size: 7135
SalI G'TCGA_C
Cuts at: 1936 4477 1936
Size: 2541 4594
SbfI CC_TGCA'GG
Cuts at: 2189 4487 2189
Size: 2298 4837
ScaI AGT'ACT
Cuts at: 3041 3347 5383 7095 3041
Size: 306 2036 1712 3081
Fragments arranged by size:
3081 2036 1712 306
SfcI C'TryA_G
Cuts at: 826 1045 2185 2335 2458 3280 4483 5618 6299
Size: 219 1140 150 123 822 1203 1135 681
Cuts at: 6299 6490 826
Size: 191 1471
Fragments arranged by size:
1471 1203 1140 1135 822 681 219 191
150 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7135
SmaI CCC'GGG
Cuts at: 1872 4462 1872
Size: 2590 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3714 4151 5239 6140 6402
Size: 197 926 597 1174 437 1088 901 262
Cuts at: 6402 820
Size: 1553
Fragments arranged by size:
1553 1174 1088 926 901 597 437 262
197
SnaBI TAC'GTA
Cuts at: 493 4926 493
Size: 4433 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7135
SphI G_CATG'C
Cuts at: 1132 4493 1132
Size: 3361 3774
SspI AAT'ATT
Cuts at: 5 52 5059 5
Size: 47 5007 2081
Fragments arranged by size:
5007 2081 47
StuI AGG'CCT
Cuts at: 1517 2627 3892 1517
Size: 1110 1265 4760
Fragments arranged by size:
4760 1265 1110
StyI C'CwwG_G
Cuts at: 513 1065 2004 2732 3002 3139 3887 4126 4574
Size: 552 939 728 270 137 748 239 448
Cuts at: 4574 513
Size: 3074
Fragments arranged by size:
3074 939 748 728 552 448 270 239
137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3783 3841 3905 4109 5162 5179 5332
Size: 1255 2203 58 64 204 1053 17 153
Cuts at: 5332 5517 5962 325
Size: 185 445 1498
Fragments arranged by size:
2203 1498 1255 1053 445 204 185 153
64 58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3039 3345 5381
Size: 274 80 33 51 1443 1062 306 2036
Cuts at: 5381 7093 96
Size: 1712 138
Fragments arranged by size:
2036 1712 1443 1062 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2782 2814 3628 3660 3758 3790 7095 7127 2782
Size: 32 814 32 98 32 3305 32 2790
Fragments arranged by size:
3305 2790 814 98 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2347 2605 1316
Size: 1031 258 5846
Fragments arranged by size:
5846 1031 258
XbaI T'CTAG_A
Cuts at: 4471 4471
Size: 7135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2611 3085 3513 4133 4280 2611
Size: 474 428 620 147 5466
Fragments arranged by size:
5466 620 474 428 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7135
XmaI C'CCGG_G
Cuts at: 1870 4460 1870
Size: 2590 4545
XmnI GAAnn'nnTTC
Cuts at: 2022 5264 2022
Size: 3242 3893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4943 276
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI