Restriction map for
pFN21AB5754
(Circular) (Six-base) MAPSORT of: pFN21AB5754.seq Check: 839 from: 1 to: 6952
pFN21AB5754 6952 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3875 3928 5015 6399 6667 1632
Size: 2243 53 1087 1384 268 1917
Fragments arranged by size:
2243 1917 1384 1087 268 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 2867 5681 2867
Size: 2814 4138
AleI CACnn'nnGTG
Cuts at: 1166 2090 1166
Size: 924 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6952
AlwNI CAG_nnn'CTG
Cuts at: 3587 4695 6267 3587
Size: 1108 1572 4272
Fragments arranged by size:
4272 1572 1108
ApaLI G'TGCA_C
Cuts at: 5009 6165 5009
Size: 1156 5796
ApoI r'AATT_y
Cuts at: 1493 2231 2652 3047 3116 3593 4261 4487 4639
Size: 738 421 395 69 477 668 226 152
Cuts at: 4639 6748 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 738 668 477 421 395 226
152 69
AseI AT'TA_AT
Cuts at: 160 1032 2757 5506 160
Size: 872 1725 2749 1606
Fragments arranged by size:
2749 1725 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 4277 1870
Size: 73 2334 4545
Fragments arranged by size:
4545 2334 73
BamHI G'GATC_C
Cuts at: 2242 4282 4649 2242
Size: 2040 367 4545
Fragments arranged by size:
4545 2040 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4273 5728 618
Size: 325 217 627 2486 1455 1842
Fragments arranged by size:
2486 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3660 4271 1391
Size: 2269 611 4072
Fragments arranged by size:
4072 2269 611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 2990 4168 928
Size: 971 575 516 1178 3712
Fragments arranged by size:
3712 1178 971 575 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5143 5177 1903
Size: 34 3206 34 3678
Fragments arranged by size:
3678 3206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3687 4844 6054 3687
Size: 1157 1210 4585
Fragments arranged by size:
4585 1210 1157
BclI T'GATC_A
Cuts at: 1548 2458 1548
Size: 910 6042
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5563 136
Size: 107 122 71 631 605 3891 1525
Fragments arranged by size:
3891 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 4369 4369
Size: 6952
Bme1580I G_kGCm'C
Cuts at: 1790 5013 6169 1790
Size: 3223 1156 2573
Fragments arranged by size:
3223 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3458 1318
Size: 2140 4812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4683 5641 442
Size: 416 53 83 399 24 3266 958 1753
Fragments arranged by size:
3266 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 3945 5612 1239
Size: 873 1833 1667 2579
Fragments arranged by size:
2579 1833 1667 873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5077 5942 6240 5077
Size: 865 298 5789
Fragments arranged by size:
5789 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 3685 5615 882
Size: 617 717 1469 1930 2219
Fragments arranged by size:
2219 1930 1469 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 2081 2558 4743 493
Size: 711 877 477 2185 2702
Fragments arranged by size:
2702 2185 877 711 477
BsaBI GATnn'nnATC
Cuts at: 2247 6946 2247
Size: 4699 2253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4759 5141 5776 275
Size: 53 83 186 936 3226 382 635 1451
Fragments arranged by size:
3226 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5385 5783 6057 6204 6615 5385
Size: 398 274 147 411 5722
Fragments arranged by size:
5722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2258 2288 1178
Size: 30 165 30 630 30 195 30 5842
Fragments arranged by size:
5842 630 195 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2765 1186
Size: 1579 5373
BseYI C'CCAG_C
Cuts at: 3461 3484 6155 6651 3461
Size: 23 2671 496 3762
Fragments arranged by size:
3762 2671 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 2952 1163
Size: 573 802 414 5163
Fragments arranged by size:
5163 802 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 5013 5098 6169 2046
Size: 2967 85 1071 2829
Fragments arranged by size:
2967 2829 1071 85
BsmI GAATG_Cn'
Cuts at: 2548 4070 4463 4556 2548
Size: 1522 393 93 4944
Fragments arranged by size:
4944 1522 393 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 3660 4271 5013 5098 6169 1391
Size: 399 256 1614 611 742 85 1071 2174
Fragments arranged by size:
2174 1614 1071 742 611 399 256 85
BspEI T'CCGG_A
Cuts at: 6615 6615
Size: 6952
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3678 4307 844
Size: 2834 629 3489
Fragments arranged by size:
3489 2834 629
BsrBI CCG'CTC
Cuts at: 1394 1823 4839 1394
Size: 429 3016 3507
Fragments arranged by size:
3507 3016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5447 5621 65
Size: 1183 4199 174 1396
Fragments arranged by size:
4199 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5596 5783 1700
Size: 110 3786 187 2869
Fragments arranged by size:
3786 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6952
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 5012 6024 5012
Size: 1012 5940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6952
BstBI TT'CG_AA
Cuts at: 5756 5756
Size: 6952
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstYI r'GATC_y
Cuts at: 1972 2017 2131 2242 3583 4282 4649 5034 5051
Size: 45 114 111 1341 699 367 385 17
Cuts at: 5051 6503 6589 6601 6947 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1341 699 385 367 346 114
111 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2607 4137 513
Size: 552 1542 1530 3328
Fragments arranged by size:
3328 1542 1530 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 4468 5262 5282 3193
Size: 1275 794 20 4863
Fragments arranged by size:
4863 1275 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 3874 4256 3874
Size: 382 6570
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3930 4399 5288
Size: 54 1425 50 275 18 2100 469 889
Cuts at: 5288 6917 8
Size: 1629 43
Fragments arranged by size:
2100 1629 1425 889 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3981 4882 1353
Size: 818 1810 901 3423
Fragments arranged by size:
3423 1810 901 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5539 5913 6059 387
Size: 1064 138 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 3875 3928 3945 5015 5612 6399
Size: 393 480 1763 53 17 1070 597 787
Cuts at: 6399 6667 1239
Size: 268 1524
Fragments arranged by size:
1763 1524 1070 787 597 480 393 268
53 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3699 3699
Size: 6952
EcoO109I rG'GnC_Cy
Cuts at: 2103 2103
Size: 6952
EcoRI G'AATT_C
Cuts at: 2652 4261 4639 2652
Size: 1609 378 4965
Fragments arranged by size:
4965 1609 378
EcoRV GAT'ATC
Cuts at: 2884 2884
Size: 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2536 3829 2122
Size: 414 1293 5245
Fragments arranged by size:
5245 1293 414
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 6099 1678
Size: 4421 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2853 2885 3308 3340 4013
Size: 32 135 32 1281 32 423 32 673
Cuts at: 4013 4045 5593 5625 5667 5699 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 1281 673 423 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 4296 4542 669
Size: 988 281 771 1587 246 3079
Fragments arranged by size:
3079 1587 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3374 6066 6250 1305
Size: 2069 2692 184 2007
Fragments arranged by size:
2692 2069 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3932 10
Size: 54 1425 343 2100 3030
Fragments arranged by size:
3030 2100 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2090 2390 4121 4910 5269 5428
Size: 648 870 54 300 1731 789 359 159
Cuts at: 5428 518
Size: 2042
Fragments arranged by size:
2042 1731 870 789 648 359 300 159
54
MspA1I CmG'CkG
Cuts at: 1606 3575 4368 5045 6193 6438 1606
Size: 1969 793 677 1148 245 2120
Fragments arranged by size:
2120 1969 1148 793 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2607 4137 513
Size: 552 1542 1530 3328
Fragments arranged by size:
3328 1542 1530 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2119 4310 5855 1132
Size: 97 890 2191 1545 2229
Fragments arranged by size:
2229 2191 1545 890 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6952
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6952
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PmlI CAC'GTG
Cuts at: 2558 2558
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 6534 6566 5021
Size: 32 1481 32 5407
Fragments arranged by size:
5407 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3753 1613
Size: 2140 4812
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PstI C_TGCA'G
Cuts at: 830 4304 830
Size: 3474 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 3575 3575
Size: 6952
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SexAI A'CCwGG_T
Cuts at: 2696 2696
Size: 6952
SfcI C'TryA_G
Cuts at: 826 1045 3576 4300 5435 6116 6307 826
Size: 219 2531 724 1135 681 191 1471
Fragments arranged by size:
2531 1471 1135 724 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 4279 1872
Size: 2407 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5056 5957 6219 820
Size: 197 926 3113 901 262 1553
Fragments arranged by size:
3113 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4310 1132
Size: 3178 3774
SspI AAT'ATT
Cuts at: 5 52 3274 4876 5
Size: 47 3222 1602 2081
Fragments arranged by size:
3222 2081 1602 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6952
StyI C'CwwG_G
Cuts at: 513 1065 2607 3417 3971 4137 4391 513
Size: 552 1542 810 554 166 254 3074
Fragments arranged by size:
3074 1542 810 554 552 254 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3164 4979 4996 5149 5334 5779 325
Size: 1255 1584 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1584 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3951 5198 6910
Size: 274 80 33 51 1443 1974 1247 1712
Cuts at: 6910 96
Size: 138
Fragments arranged by size:
1974 1712 1443 1247 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 6912 6944 2036
Size: 32 4844 32 2044
Fragments arranged by size:
4844 2044 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6952
XbaI T'CTAG_A
Cuts at: 2398 4288 2398
Size: 1890 5062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2293
Size: 6952
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 4277 1870
Size: 2407 4545
XmnI GAAnn'nnTTC
Cuts at: 2394 2471 5081 2394
Size: 77 2610 4265
Fragments arranged by size:
4265 2610 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI