Restriction map for
pFN21AB5741
(Circular) (Six-base) MAPSORT of: pFN21AB5741.seq Check: 6910 from: 1 to: 7102
pFN21AB5741 7102 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3902 3902
Size: 7102
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2322 4912 278
Size: 53 83 186 936 786 2590 2468
Fragments arranged by size:
2590 2468 936 786 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4445 1937
Size: 2508 4594
Acc65I G'GTAC_C
Cuts at: 2592 4423 2592
Size: 1831 5271
AclI AA'CG_TT
Cuts at: 1563 5229 5602 1563
Size: 3666 373 3063
Fragments arranged by size:
3666 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 5165 6549 6817 1632
Size: 1640 1893 1384 268 1917
Fragments arranged by size:
1917 1893 1640 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6905
AflIII A'CryG_T
Cuts at: 1203 6001 6978 1203
Size: 4798 977 1327
Fragments arranged by size:
4798 1327 977
AgeI A'CCGG_T
Cuts at: 5933 5933
Size: 7102
AhdI GACnn_n'nnGTC
Cuts at: 5831 5831
Size: 7102
AleI CACnn'nnGTG
Cuts at: 1166 4007 4019 4091 1166
Size: 2841 12 72 4177
Fragments arranged by size:
4177 2841 72 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2794 3345 1184
Size: 1610 551 4941
Fragments arranged by size:
4941 1610 551
AlwNI CAG_nnn'CTG
Cuts at: 3538 4845 6417 3538
Size: 1307 1572 4223
Fragments arranged by size:
4223 1572 1307
ApaI G_GGCC'C
Cuts at: 2017 4297 2017
Size: 2280 4822
ApaLI G'TGCA_C
Cuts at: 5159 6315 5159
Size: 1156 5946
ApoI r'AATT_y
Cuts at: 1493 3043 4411 4637 4789 6898 1493
Size: 1550 1368 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 1368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3120 5656 160
Size: 872 2088 2536 1606
Fragments arranged by size:
2536 2088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
AvaI C'yCGr_G
Cuts at: 1870 1943 4427 1870
Size: 73 2484 4545
Fragments arranged by size:
4545 2484 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2576 2609 3118 3151 2576
Size: 33 509 33 6527
Fragments arranged by size:
6527 509 33 33
BamHI G'GATC_C
Cuts at: 4432 4799 4432
Size: 367 6735
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2486 2584 2592 3488
Size: 325 217 627 233 466 98 8 896
Cuts at: 3488 4423 5878 618
Size: 935 1455 1842
Fragments arranged by size:
1842 1455 935 896 627 466 325 233
217 98 8
BanII G_rGCy'C
Cuts at: 1391 2017 2049 2249 2306 4101 4297 4421 1391
Size: 626 32 200 57 1795 196 124 4072
Fragments arranged by size:
4072 1795 626 200 196 124 57 32
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 7102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6131
BbvCI CC'TCA_GC
Cuts at: 2285 2285
Size: 7102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2476 2510 2912 2946 5293
Size: 34 50 34 455 34 402 34 2347
Cuts at: 5293 5327 1903
Size: 34 3678
Fragments arranged by size:
3678 2347 455 402 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3146 3229 4994 6204 3146
Size: 83 1765 1210 4044
Fragments arranged by size:
4044 1765 1210 83
BclI T'GATC_A
Cuts at: 1548 3999 1548
Size: 2451 4651
BfrBI ATG'CAT
Cuts at: 3125 3905 3125
Size: 780 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5713 136
Size: 107 122 71 631 605 4041 1525
Fragments arranged by size:
4041 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3630 3804 7097 3630
Size: 174 3293 3635
Fragments arranged by size:
3635 3293 174
BlpI GC'TnA_GC
Cuts at: 4519 4519
Size: 7102
Bme1580I G_kGCm'C
Cuts at: 1790 2017 2489 2847 3491 3825 3890 4297 5163
Size: 227 472 358 644 334 65 407 866
Cuts at: 5163 6319 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 866 644 472 407 358 334
227 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4098 4833 5791
Size: 416 53 83 399 24 2681 735 958
Cuts at: 5791 442
Size: 1753
Fragments arranged by size:
2681 1753 958 735 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2750 3383 5762 1239
Size: 1511 633 2379 2579
Fragments arranged by size:
2579 2379 1511 633
Bpu10I CC'TnA_GC
Cuts at: 2285 3694 2285
Size: 1409 5693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5227 6092 6390 5227
Size: 865 298 5939
Fragments arranged by size:
5939 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 5765 882
Size: 617 582 3684 2219
Fragments arranged by size:
3684 2219 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 3390 4893 493
Size: 711 2186 1503 2702
Fragments arranged by size:
2702 2186 1503 711
BsaBI GATnn'nnATC
Cuts at: 3183 7096 3183
Size: 3913 3189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2319 4909 5291
Size: 53 83 186 936 488 298 2590 382
Cuts at: 5291 5926 275
Size: 635 1451
Fragments arranged by size:
2590 1451 936 635 488 382 298 186
83 53
BsaWI w'CCGG_w
Cuts at: 2475 2529 3138 4254 5535 5933 6207 6354 6765
Size: 54 609 1116 1281 398 274 147 411
Cuts at: 6765 2475
Size: 2812
Fragments arranged by size:
2812 1281 1116 609 411 398 274 147
54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2865 2895 3311 3341 3587
Size: 30 165 30 1462 30 416 30 246
Cuts at: 3587 3617 3647 3677 4127 4157 1178
Size: 30 30 30 450 30 4123
Fragments arranged by size:
4123 1462 450 416 246 165 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2287 2516 1186
Size: 1101 229 5772
Fragments arranged by size:
5772 1101 229
BseYI C'CCAG_C
Cuts at: 2248 2313 3305 6305 6801 2248
Size: 65 992 3000 496 2549
Fragments arranged by size:
3000 2549 992 496 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 3370 3490 3589 1163
Size: 573 1167 467 120 99 4676
Fragments arranged by size:
4676 1167 573 467 120 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3341 4255 4552 5313 6341 1540
Size: 275 186 1340 914 297 761 1028 2301
Fragments arranged by size:
2301 1340 1028 914 761 297 275 186
BsiHKAI G_wGCw'C
Cuts at: 2153 3605 4101 5163 5248 6319 2153
Size: 1452 496 1062 85 1071 2936
Fragments arranged by size:
2936 1452 1071 1062 496 85
BsmI GAATG_Cn'
Cuts at: 3728 4613 4706 3728
Size: 885 93 6124
Fragments arranged by size:
6124 885 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2049 2153 2249 2306 2489 2847
Size: 399 227 32 104 96 57 183 358
Cuts at: 2847 3491 3605 3825 3890 4101 4297 4421 5163
Size: 644 114 220 65 211 196 124 742
Cuts at: 5163 5248 6319 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 358 227 220
211 196 183 124 114 104 96 85
65 57 32
BspEI T'CCGG_A
Cuts at: 2475 6765 2475
Size: 4290 2812
BspHI T'CATG_A
Cuts at: 4077 4991 6721 4077
Size: 914 1730 4458
Fragments arranged by size:
4458 1730 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2063 3212 3728 3902 3980 4457 844
Size: 1219 1149 516 174 78 477 3489
Fragments arranged by size:
3489 1219 1149 516 477 174 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 4989 1394
Size: 429 1618 1548 3507
Fragments arranged by size:
3507 1618 1548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5597 5771 65
Size: 1183 4349 174 1396
Fragments arranged by size:
4349 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3340 5746 5933 1700
Size: 110 1530 2406 187 2869
Fragments arranged by size:
2869 2406 1530 187 110
BsrGI T'GTAC_A
Cuts at: 96 3036 96
Size: 2940 4162
BssHII G'CGCG_C
Cuts at: 1925 2052 1925
Size: 127 6975
BssSI C'ACGA_G
Cuts at: 5162 6174 5162
Size: 1012 6090
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3495 3600 794
Size: 2701 105 4296
Fragments arranged by size:
4296 2701 105
BstBI TT'CG_AA
Cuts at: 5906 5906
Size: 7102
BstEII G'GTnAC_C
Cuts at: 1184 3373 1184
Size: 2189 4913
BstXI CCAn_nnnn'nTGG
Cuts at: 2944 4177 2944
Size: 1233 5869
BstYI r'GATC_y
Cuts at: 1972 3178 3630 3804 4432 4799 5184 5201 6653
Size: 1206 452 174 628 367 385 17 1452
Cuts at: 6653 6739 6751 7097 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1206 628 452 385 367 346
174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2270 2742 3174 4130 2270
Size: 472 432 956 5242
Fragments arranged by size:
5242 956 472 432
BtgI C'CryG_G
Cuts at: 513 1065 2009 2174 3700 513
Size: 552 944 165 1526 3915
Fragments arranged by size:
3915 1526 944 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2836 487
Size: 683 56 1610 4753
Fragments arranged by size:
4753 1610 683 56
BtsI GCAGTG_nn'
Cuts at: 2810 2928 3018 3712 3845 4618 5412 5432 2810
Size: 118 90 694 133 773 794 20 4480
Fragments arranged by size:
4480 794 773 694 133 118 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7067
DraI TTT'AAA
Cuts at: 4406 4406
Size: 7102
DraIII CAC_nnn'GTG
Cuts at: 3495 4351 3495
Size: 856 6246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6109 809
Size: 507 4793 1802
Fragments arranged by size:
4793 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3338 3799
Size: 54 1425 50 275 18 1066 442 461
Cuts at: 3799 4549 5438 7067 8
Size: 750 889 1629 43
Fragments arranged by size:
1629 1425 1066 889 750 461 442 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3338 4549 1537
Size: 275 1526 1211 4090
Fragments arranged by size:
4090 1526 1211 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3194 5032 1353
Size: 1841 1838 3423
Fragments arranged by size:
3423 1841 1838
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2645 5689 6063 6209 387
Size: 1064 138 1056 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 1056 374 146 138
EcoICRI GAG'CTC
Cuts at: 4099 4099
Size: 7102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2750 3272 3383 5165 5762 6549 6817
Size: 393 1118 522 111 1782 597 787 268
Cuts at: 6817 1239
Size: 1524
Fragments arranged by size:
1782 1524 1118 787 597 522 393 268
111
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 3670 2912
Size: 758 6344
EcoO109I rG'GnC_Cy
Cuts at: 2014 3444 3861 4293 2014
Size: 1430 417 432 4823
Fragments arranged by size:
4823 1430 432 417
EcoRI G'AATT_C
Cuts at: 4411 4789 4411
Size: 378 6724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2479 2395
Size: 84 7018
FspI TGC'GCA
Cuts at: 5608 5608
Size: 7102
HaeII r_GCGC'y
Cuts at: 1678 2024 3426 6249 1678
Size: 346 1402 2823 2531
Fragments arranged by size:
2823 2531 1402 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2935 2939 2967 2971 3077
Size: 32 135 32 1363 4 28 4 106
Cuts at: 3077 3109 3203 3235 3254 3286 3647 3679 3912
Size: 32 94 32 19 32 361 32 233
Cuts at: 3912 3944 5743 5775 5817 5849 1373
Size: 32 1799 32 42 32 2626
Fragments arranged by size:
2626 1799 1363 361 233 135 106 94
42 32 32 32 32 32 32 32
32 32 28 19 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4446 4692 669
Size: 988 281 2508 246 3079
Fragments arranged by size:
3079 2508 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7102
HpaI GTT'AAC
Cuts at: 4692 4692
Size: 7102
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 7102
KpnI G_GTAC'C
Cuts at: 2596 4427 2596
Size: 1831 5271
MfeI C'AATT_G
Cuts at: 4701 4701
Size: 7102
MluI A'CGCG_T
Cuts at: 6978 6978
Size: 7102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2961 4140 6216 6400 1305
Size: 1656 1179 2076 184 2007
Fragments arranged by size:
2076 2007 1656 1179 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2898 3801 10
Size: 54 1425 343 1066 903 3311
Fragments arranged by size:
3311 1425 1066 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 4007 4019 4091 5060 5419 5578
Size: 648 1161 1680 12 72 969 359 159
Cuts at: 5578 518
Size: 2042
Fragments arranged by size:
2042 1680 1161 969 648 359 159 72
12
MspA1I CmG'CkG
Cuts at: 1606 2011 2026 3573 3591 4518 5195 6343 6588
Size: 405 15 1547 18 927 677 1148 245
Cuts at: 6588 1606
Size: 2120
Fragments arranged by size:
2120 1547 1148 927 677 405 245 18
15
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 7102
NcoI C'CATG_G
Cuts at: 513 1065 3700 513
Size: 552 2635 3915
Fragments arranged by size:
3915 2635 552
NdeI CA'TA_TG
Cuts at: 387 2084 387
Size: 1697 5405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7102
NotI GC'GGCC_GC
Cuts at: 4549 4549
Size: 7102
NsiI A_TGCA'T
Cuts at: 3127 3907 3127
Size: 780 6322
NspI r_CATG'y
Cuts at: 1132 1229 3326 3786 3909 4460 6005 1132
Size: 97 2097 460 123 551 1545 2229
Fragments arranged by size:
2229 2097 1545 551 460 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7102
PciI A'CATG_T
Cuts at: 6001 6001
Size: 7102
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2894 4145 1108
Size: 1786 1251 4065
Fragments arranged by size:
4065 1786 1251
PfoI T'CCnGG_A
Cuts at: 2723 3351 2723
Size: 628 6474
PmeI GTTT'AAAC
Cuts at: 4406 4406
Size: 7102
PmlI CAC'GTG
Cuts at: 3390 3390
Size: 7102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5171 5203 6684 6716 5171
Size: 32 1481 32 5557
Fragments arranged by size:
5557 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3444 3444
Size: 7102
PshAI GACnn'nnGTC
Cuts at: 1613 3449 1613
Size: 1836 5266
PsiI TTA'TAA
Cuts at: 4672 4672
Size: 7102
PspOMI G'GGCC_C
Cuts at: 2013 4293 2013
Size: 2280 4822
PstI C_TGCA'G
Cuts at: 830 2311 3401 3590 3812 4454 830
Size: 1481 1090 189 222 642 3478
Fragments arranged by size:
3478 1481 1090 642 222 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7102
PvuII CAG'CTG
Cuts at: 3591 3591
Size: 7102
SacI G_AGCT'C
Cuts at: 4101 4101
Size: 7102
SacII CC_GC'GG
Cuts at: 2012 2012
Size: 7102
SalI G'TCGA_C
Cuts at: 1936 4444 1936
Size: 2508 4594
SbfI CC_TGCA'GG
Cuts at: 4454 4454
Size: 7102
ScaI AGT'ACT
Cuts at: 2763 5350 7062 2763
Size: 2587 1712 2803
Fragments arranged by size:
2803 2587 1712
SexAI A'CCwGG_T
Cuts at: 3491 3491
Size: 7102
SfcI C'TryA_G
Cuts at: 826 1045 2307 2387 3397 3586 3808 4450 5585
Size: 219 1262 80 1010 189 222 642 1135
Cuts at: 5585 6266 6457 826
Size: 681 191 1471
Fragments arranged by size:
1471 1262 1135 1010 681 642 222 219
191 189 80
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 7102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7102
SmaI CCC'GGG
Cuts at: 1872 4429 1872
Size: 2557 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5206 6107 6369 820
Size: 197 926 3263 901 262 1553
Fragments arranged by size:
3263 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4893 493
Size: 4400 2702
SpeI A'CTAG_T
Cuts at: 152 2090 152
Size: 1938 5164
SphI G_CATG'C
Cuts at: 1132 3909 4460 1132
Size: 2777 551 3774
Fragments arranged by size:
3774 2777 551
SspI AAT'ATT
Cuts at: 5 52 3103 5026 5
Size: 47 3051 1923 2081
Fragments arranged by size:
3051 2081 1923 47
StuI AGG'CCT
Cuts at: 1517 2877 1517
Size: 1360 5742
StyI C'CwwG_G
Cuts at: 513 1065 3700 4065 4541 513
Size: 552 2635 365 476 3074
Fragments arranged by size:
3074 2635 552 476 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2148 3997 5129 5146 5299 5484 5929
Size: 1255 568 1849 1132 17 153 185 445
Cuts at: 5929 325
Size: 1498
Fragments arranged by size:
1849 1498 1255 1132 568 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2761 2925
Size: 274 80 33 51 1443 447 337 164
Cuts at: 2925 3036 5348 7060 96
Size: 111 2312 1712 138
Fragments arranged by size:
2312 1712 1443 447 337 274 164 138
111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2865 2897 3057 3089 3713 3745 4127 4159 7062
Size: 32 160 32 624 32 382 32 2903
Cuts at: 7062 7094 2865
Size: 32 2873
Fragments arranged by size:
2903 2873 624 382 160 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4073 1316
Size: 2757 4345
XbaI T'CTAG_A
Cuts at: 4438 4438
Size: 7102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2142 4348 2142
Size: 2206 4896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7102
XmaI C'CCGG_G
Cuts at: 1870 4427 1870
Size: 2557 4545
XmnI GAAnn'nnTTC
Cuts at: 5231 5231
Size: 7102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2320 4910 276
Size: 53 83 186 936 786 2590 2468
Fragments arranged by size:
2590 2468 936 786 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BplI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI
PsrI RsrII SanDI SapI SfiI SrfI SwaI