Restriction map for
pFN21AB5739
(Circular) (Six-base) MAPSORT of: pFN21AB5739.seq Check: 5425 from: 1 to: 7072
pFN21AB5739 7072 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4232 4232
Size: 7072
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4882 278
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4415 1937
Size: 2478 4594
Acc65I G'GTAC_C
Cuts at: 4393 4393
Size: 7072
AclI AA'CG_TT
Cuts at: 1563 3759 5199 5572 1563
Size: 2196 1440 373 3063
Fragments arranged by size:
3063 2196 1440 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2945 3350 3358 3668 3842 5135 6519
Size: 735 578 405 8 310 174 1293 1384
Cuts at: 6519 6787 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1293 735 578 405 310 268
174 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6875
AflIII A'CryG_T
Cuts at: 1203 3084 5971 6948 1203
Size: 1881 2887 977 1327
Fragments arranged by size:
2887 1881 1327 977
AgeI A'CCGG_T
Cuts at: 5903 5903
Size: 7072
AhdI GACnn_n'nnGTC
Cuts at: 4300 5801 4300
Size: 1501 5571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7072
AlwNI CAG_nnn'CTG
Cuts at: 2470 3284 3421 4021 4224 4242 4815 6387 2470
Size: 814 137 600 203 18 573 1572 3155
Fragments arranged by size:
3155 1572 814 600 573 203 137 18
ApaLI G'TGCA_C
Cuts at: 5129 6285 5129
Size: 1156 5916
ApoI r'AATT_y
Cuts at: 1493 2223 2304 2430 4381 4607 4759 6868 1493
Size: 730 81 126 1951 226 152 2109 1697
Fragments arranged by size:
2109 1951 1697 730 226 152 126 81
AseI AT'TA_AT
Cuts at: 160 1032 5626 160
Size: 872 4594 1606
Fragments arranged by size:
4594 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
AvaI C'yCGr_G
Cuts at: 1870 1943 3588 4397 1870
Size: 73 1645 809 4545
Fragments arranged by size:
4545 1645 809 73
BamHI G'GATC_C
Cuts at: 4402 4769 4402
Size: 367 6705
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 3964 4393 5848 618
Size: 325 217 627 227 1950 429 1455 1842
Fragments arranged by size:
1950 1842 1455 627 429 325 227 217
BanII G_rGCy'C
Cuts at: 1391 4338 4391 1391
Size: 2947 53 4072
Fragments arranged by size:
4072 2947 53
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 7072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2615 928
Size: 971 716 5385
Fragments arranged by size:
5385 971 716
BbvCI CC'TCA_GC
Cuts at: 2229 2229
Size: 7072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5263 5297 1903
Size: 34 3326 34 3678
Fragments arranged by size:
3678 3326 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2519 2716 2767 4964 6174 2519
Size: 197 51 2197 1210 3417
Fragments arranged by size:
3417 2197 1210 197 51
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7072
BfrBI ATG'CAT
Cuts at: 3127 3767 3127
Size: 640 6432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2069 5683
Size: 107 122 71 631 605 337 60 3614
Cuts at: 5683 136
Size: 1525
Fragments arranged by size:
3614 1525 631 605 337 122 107 71
60
BglII A'GATC_T
Cuts at: 7067 7067
Size: 7072
BlpI GC'TnA_GC
Cuts at: 4489 4489
Size: 7072
Bme1580I G_kGCm'C
Cuts at: 1790 2948 3969 5133 6289 1790
Size: 1158 1021 1164 1156 2573
Fragments arranged by size:
2573 1164 1158 1156 1021
BmgBI CAC'GTC
Cuts at: 1318 3087 1318
Size: 1769 5303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3103 4803 5761
Size: 416 53 83 399 24 1686 1700 958
Cuts at: 5761 442
Size: 1753
Fragments arranged by size:
1753 1700 1686 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7072
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4255 4255
Size: 7072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 3701 5732 1239
Size: 816 1646 2031 2579
Fragments arranged by size:
2579 2031 1646 816
Bpu10I CC'TnA_GC
Cuts at: 2229 2229
Size: 7072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2282 2524 2854 3431 5197 6062 6360 2225
Size: 57 242 330 577 1766 865 298 2937
Fragments arranged by size:
2937 1766 865 577 330 298 242 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5735 882
Size: 617 4236 2219
Fragments arranged by size:
4236 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4012 4863 493
Size: 711 2808 851 2702
Fragments arranged by size:
2808 2702 851 711
BsaBI GATnn'nnATC
Cuts at: 2453 7066 2453
Size: 4613 2459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3184 4879 5261
Size: 53 83 186 936 482 1169 1695 382
Cuts at: 5261 5896 275
Size: 635 1451
Fragments arranged by size:
1695 1451 1169 936 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5505 5903 6177 6324 6735 5505
Size: 398 274 147 411 5842
Fragments arranged by size:
5842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4259 4289 1178
Size: 30 165 30 2856 30 3961
Fragments arranged by size:
3961 2856 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2301 3050 3080 4346 4382 1186
Size: 1042 73 749 30 1266 36 3876
Fragments arranged by size:
3876 1266 1042 749 73 36 30
BseYI C'CCAG_C
Cuts at: 2027 3099 6275 6771 2027
Size: 1072 3176 496 2328
Fragments arranged by size:
3176 2328 1072 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2231 4034 4187 1163
Size: 573 453 42 1803 153 4048
Fragments arranged by size:
4048 1803 573 453 153 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2044 2057 2095 2099 2149 4522
Size: 275 186 43 13 38 4 50 2373
Cuts at: 4522 5283 6311 1540
Size: 761 1028 2301
Fragments arranged by size:
2373 2301 1028 761 275 186 50 43
38 13 4
BsiHKAI G_wGCw'C
Cuts at: 3048 3156 3669 3717 4131 5133 5218 6289 3048
Size: 108 513 48 414 1002 85 1071 3831
Fragments arranged by size:
3831 1071 1002 513 414 108 85 48
BsmI GAATG_Cn'
Cuts at: 2883 3274 3767 3941 4583 4676 2883
Size: 391 493 174 642 93 5279
Fragments arranged by size:
5279 642 493 391 174 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2044 2044
Size: 7072
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2948 3048 3156 3669 3717 3969 4131
Size: 399 1158 100 108 513 48 252 162
Cuts at: 4131 4338 4391 5133 5218 6289 1391
Size: 207 53 742 85 1071 2174
Fragments arranged by size:
2174 1158 1071 742 513 399 252 207
162 108 100 85 53 48
BspEI T'CCGG_A
Cuts at: 6735 6735
Size: 7072
BspHI T'CATG_A
Cuts at: 3543 4961 6691 3543
Size: 1418 1730 3924
Fragments arranged by size:
3924 1730 1418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3529 4113 4182 4209 4232 4427 844
Size: 2685 584 69 27 23 195 3489
Fragments arranged by size:
3489 2685 584 195 69 27 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2902 4090 4959 1394
Size: 429 1079 1188 869 3507
Fragments arranged by size:
3507 1188 1079 869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5567 5741 65
Size: 1183 4319 174 1396
Fragments arranged by size:
4319 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2039 2094 2144 4296 5716 5903
Size: 110 207 22 55 50 2152 1420 187
Cuts at: 5903 1700
Size: 2869
Fragments arranged by size:
2869 2152 1420 207 187 110 55 50
22
BsrGI T'GTAC_A
Cuts at: 96 2531 3138 96
Size: 2435 607 4030
Fragments arranged by size:
4030 2435 607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7072
BssSI C'ACGA_G
Cuts at: 3386 5132 6144 3386
Size: 1746 1012 4314
Fragments arranged by size:
4314 1746 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4201 794
Size: 3407 3665
BstBI TT'CG_AA
Cuts at: 5876 5876
Size: 7072
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7072
BstXI CCAn_nnnn'nTGG
Cuts at: 3859 3859
Size: 7072
BstYI r'GATC_y
Cuts at: 1972 4402 4769 5154 5171 6623 6709 6721 7067
Size: 2430 367 385 17 1452 86 12 346
Cuts at: 7067 1972
Size: 1977
Fragments arranged by size:
2430 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3462 3462
Size: 7072
BtgI C'CryG_G
Cuts at: 513 1065 2004 2099 2322 3298 513
Size: 552 939 95 223 976 4287
Fragments arranged by size:
4287 976 939 552 223 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6333
Fragments arranged by size:
6333 683 56
BtsI GCAGTG_nn'
Cuts at: 4588 5382 5402 4588
Size: 794 20 6258
Fragments arranged by size:
6258 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2195 2200 2230 2235 529
Size: 35 1631 5 30 5 5366
Fragments arranged by size:
5366 1631 35 30 5 5
DraI TTT'AAA
Cuts at: 4376 4376
Size: 7072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6079 809
Size: 507 4763 1802
Fragments arranged by size:
4763 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2092 2096
Size: 54 1425 50 275 18 211 51 4
Cuts at: 2096 2146 3027 4519 5408 7037 8
Size: 50 881 1492 889 1629 43
Fragments arranged by size:
1629 1492 1425 889 881 275 211 54
51 50 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2041 2092 2096 2146 4519 1537
Size: 275 229 51 4 50 2373 4090
Fragments arranged by size:
4090 2373 275 229 51 50 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2343 2822 2967 3506 3992 5002 1353
Size: 966 24 479 145 539 486 1010 3423
Fragments arranged by size:
3423 1010 966 539 486 479 145 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2097 3980 5659 6033 6179 387
Size: 1064 138 508 1883 1679 374 146 1280
Fragments arranged by size:
1883 1679 1280 1064 508 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2367 2945 3350 3358 3668 3701
Size: 393 423 312 578 405 8 310 33
Cuts at: 3701 3842 5135 5732 6519 6787 1239
Size: 141 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 597 578 423 405 393
312 310 268 141 33 8
EcoNI CCTnn'n_nnAGG
Cuts at: 4205 4205
Size: 7072
EcoO109I rG'GnC_Cy
Cuts at: 2152 2843 2152
Size: 691 6381
EcoRI G'AATT_C
Cuts at: 2223 4381 4759 2223
Size: 2158 378 4536
Fragments arranged by size:
4536 2158 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2587 2632 2848 2891 3535 2518
Size: 69 45 216 43 644 6055
Fragments arranged by size:
6055 644 216 69 45 43
FseI GG_CCGG'CC
Cuts at: 2098 2098
Size: 7072
FspI TGC'GCA
Cuts at: 5578 5578
Size: 7072
HaeII r_GCGC'y
Cuts at: 1678 2018 6219 1678
Size: 340 4201 2531
Fragments arranged by size:
4201 2531 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2474 2506 3169
Size: 32 135 32 620 32 250 32 663
Cuts at: 3169 3201 3686 3718 3914 3946 4259 4286 4291
Size: 32 485 32 196 32 313 27 5
Cuts at: 4291 4318 5713 5745 5787 5819 1373
Size: 27 1395 32 42 32 2626
Fragments arranged by size:
2626 1395 663 620 485 313 250 196
135 42 32 32 32 32 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 4416 4662 669
Size: 988 281 1002 1476 246 3079
Fragments arranged by size:
3079 1476 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7072
HpaI GTT'AAC
Cuts at: 4662 4662
Size: 7072
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 7072
KpnI G_GTAC'C
Cuts at: 4397 4397
Size: 7072
MfeI C'AATT_G
Cuts at: 4671 4671
Size: 7072
MluI A'CGCG_T
Cuts at: 6948 6948
Size: 7072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2695 2949 3876 6186 6370 1305
Size: 1390 254 927 2310 184 2007
Fragments arranged by size:
2310 2007 1390 927 254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3029 10
Size: 54 1425 343 1197 4053
Fragments arranged by size:
4053 1425 1197 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 3974 4010 5030 5389 5548 518
Size: 648 2694 114 36 1020 359 159 2042
Fragments arranged by size:
2694 2042 1020 648 359 159 114 36
MspA1I CmG'CkG
Cuts at: 1606 2101 2125 2140 2233 2855 3103 4041 4120
Size: 495 24 15 93 622 248 938 79
Cuts at: 4120 4488 5165 6313 6558 1606
Size: 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 622 495 368 248
245 93 79 24 15
NaeI GCC'GGC
Cuts at: 2041 2096 2041
Size: 55 7017
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 7072
NcoI C'CATG_G
Cuts at: 513 1065 2004 2322 3298 513
Size: 552 939 318 976 4287
Fragments arranged by size:
4287 976 939 552 318
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7072
NgoMIV G'CCGG_C
Cuts at: 2039 2094 2039
Size: 55 7017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7072
NotI GC'GGCC_GC
Cuts at: 4519 4519
Size: 7072
NsiI A_TGCA'T
Cuts at: 3129 3769 3129
Size: 640 6432
NspI r_CATG'y
Cuts at: 1132 1229 3859 4430 5975 1132
Size: 97 2630 571 1545 2229
Fragments arranged by size:
2630 2229 1545 571 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7072
PciI A'CATG_T
Cuts at: 5971 5971
Size: 7072
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7072
PmeI GTTT'AAAC
Cuts at: 4376 4376
Size: 7072
PmlI CAC'GTG
Cuts at: 4012 4012
Size: 7072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 6654 6686 5141
Size: 32 1481 32 5527
Fragments arranged by size:
5527 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7072
PsiI TTA'TAA
Cuts at: 4642 4642
Size: 7072
PstI C_TGCA'G
Cuts at: 830 2124 2854 3540 4119 4424 830
Size: 1294 730 686 579 305 3478
Fragments arranged by size:
3478 1294 730 686 579 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7072
PvuII CAG'CTG
Cuts at: 2125 2140 2233 2855 3103 4041 2125
Size: 15 93 622 248 938 5156
Fragments arranged by size:
5156 938 622 248 93 15
RsrII CG'GwC_CG
Cuts at: 4294 4294
Size: 7072
SacII CC_GC'GG
Cuts at: 2102 2102
Size: 7072
SalI G'TCGA_C
Cuts at: 1936 4414 1936
Size: 2478 4594
SapI GCTCTTCn'nnn_
Cuts at: 2822 2822
Size: 7072
SbfI CC_TGCA'GG
Cuts at: 4424 4424
Size: 7072
ScaI AGT'ACT
Cuts at: 3398 5320 7032 3398
Size: 1922 1712 3438
Fragments arranged by size:
3438 1922 1712
SexAI A'CCwGG_T
Cuts at: 3195 3900 3195
Size: 705 6367
SfcI C'TryA_G
Cuts at: 826 1045 2120 2850 3322 3536 4115 4420 5555
Size: 219 1075 730 472 214 579 305 1135
Cuts at: 5555 6236 6427 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1075 730 681 579 472 305
219 214 191
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 7072
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7072
SmaI CCC'GGG
Cuts at: 1872 4399 1872
Size: 2527 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2297 2503 2833 3446 5176
Size: 197 926 297 57 206 330 613 1730
Cuts at: 5176 6077 6339 820
Size: 901 262 1553
Fragments arranged by size:
1730 1553 926 901 613 330 297 262
206 197 57
SnaBI TAC'GTA
Cuts at: 493 4863 493
Size: 4370 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7072
SphI G_CATG'C
Cuts at: 1132 4430 1132
Size: 3298 3774
SspI AAT'ATT
Cuts at: 5 52 4996 5
Size: 47 4944 2081
Fragments arranged by size:
4944 2081 47
StuI AGG'CCT
Cuts at: 1517 2443 3657 1517
Size: 926 1214 4932
Fragments arranged by size:
4932 1214 926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2493 3298 4511 513
Size: 552 939 318 171 805 1213 3074
Fragments arranged by size:
3074 1213 939 805 552 318 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2639 3903 5099 5116 5269 5454 5899
Size: 1255 1059 1264 1196 17 153 185 445
Cuts at: 5899 325
Size: 1498
Fragments arranged by size:
1498 1264 1255 1196 1059 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2531 3138 3396
Size: 274 80 33 51 1443 554 607 258
Cuts at: 3396 5318 7030 96
Size: 1922 1712 138
Fragments arranged by size:
1922 1712 1443 607 554 274 258 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 7032 7064 3680
Size: 32 3320 32 3688
Fragments arranged by size:
3688 3320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4273 1316
Size: 2957 4115
XbaI T'CTAG_A
Cuts at: 4408 4408
Size: 7072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2767 2329
Size: 438 6634
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7072
XmaI C'CCGG_G
Cuts at: 1870 4397 1870
Size: 2527 4545
XmnI GAAnn'nnTTC
Cuts at: 2612 5201 2612
Size: 2589 4483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4880 276
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
DraIII EcoICRI EcoRV FspAI NruI PacI PfoI PpuMI
PspOMI PspXI PsrI SacI SanDI SfiI SrfI SwaI