Restriction map for
pFN21AB5738
(Circular) (Six-base) MAPSORT of: pFN21AB5738.seq Check: 5377 from: 1 to: 6925
pFN21AB5738 6925 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4735 278
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2453 4268 1937
Size: 516 1815 4594
Fragments arranged by size:
4594 1815 516
Acc65I G'GTAC_C
Cuts at: 3885 4039 4246 3885
Size: 154 207 6564
Fragments arranged by size:
6564 207 154
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 3191 3483 4988 6372 6640 1632
Size: 612 947 292 1505 1384 268 1917
Fragments arranged by size:
1917 1505 1384 947 612 292 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 2608 5824 6801 1203
Size: 1405 3216 977 1327
Fragments arranged by size:
3216 1405 1327 977
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 3064 5654 3064
Size: 2590 4335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6925
AlwNI CAG_nnn'CTG
Cuts at: 3323 3369 4668 6240 3323
Size: 46 1299 1572 4008
Fragments arranged by size:
4008 1572 1299 46
ApaLI G'TGCA_C
Cuts at: 4982 6138 4982
Size: 1156 5769
ApoI r'AATT_y
Cuts at: 1493 2558 2720 2741 4052 4234 4460 4612 6721
Size: 1065 162 21 1311 182 226 152 2109
Cuts at: 6721 1493
Size: 1697
Fragments arranged by size:
2109 1697 1311 1065 226 182 162 152
21
AseI AT'TA_AT
Cuts at: 160 1032 2387 5479 160
Size: 872 1355 3092 1606
Fragments arranged by size:
3092 1606 1355 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2889 2991 4250 1870
Size: 73 946 102 1259 4545
Fragments arranged by size:
4545 1259 946 102 73
AvrII C'CTAG_G
Cuts at: 2476 2476
Size: 6925
BamHI G'GATC_C
Cuts at: 4255 4622 4255
Size: 367 6558
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 3885 4039 4129 4246
Size: 325 217 627 998 1100 154 90 117
Cuts at: 4246 5701 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1100 998 627 325 217 154
117 90
BanII G_rGCy'C
Cuts at: 1391 4244 1391
Size: 2853 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3055 3312 3629 928
Size: 971 1156 257 317 4224
Fragments arranged by size:
4224 1156 971 317 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5116 5150 1903
Size: 34 3179 34 3678
Fragments arranged by size:
3678 3179 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2425 4817 6027 2425
Size: 2392 1210 3323
Fragments arranged by size:
3323 2392 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6925
BfrBI ATG'CAT
Cuts at: 2569 3274 2569
Size: 705 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5536 136
Size: 107 122 71 631 605 337 3527 1525
Fragments arranged by size:
3527 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2635 6920 2635
Size: 4285 2640
BlpI GC'TnA_GC
Cuts at: 4342 4342
Size: 6925
Bme1580I G_kGCm'C
Cuts at: 1790 4986 6142 1790
Size: 3196 1156 2573
Fragments arranged by size:
3196 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3338 4656 5614
Size: 416 53 83 399 24 1921 1318 958
Cuts at: 5614 442
Size: 1753
Fragments arranged by size:
1921 1753 1318 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5585 1239
Size: 4346 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3075 3120 3917 5050 5915 6213 3075
Size: 45 797 1133 865 298 3787
Fragments arranged by size:
3787 1133 865 797 298 45
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5588 882
Size: 617 4089 2219
Fragments arranged by size:
4089 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3802 4716 493
Size: 711 2598 914 2702
Fragments arranged by size:
2702 2598 914 711
BsaBI GATnn'nnATC
Cuts at: 6919 6919
Size: 6925
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4732 5114 5749 275
Size: 53 83 186 936 3199 382 635 1451
Fragments arranged by size:
3199 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2788 5358 5756 6030 6177 6588 2788
Size: 2570 398 274 147 411 3125
Fragments arranged by size:
3125 2570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 1178
Size: 30 165 30 709 30 5961
Fragments arranged by size:
5961 709 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2133 3609 1186
Size: 877 70 1476 4502
Fragments arranged by size:
4502 1476 877 70
BseYI C'CCAG_C
Cuts at: 6128 6624 6128
Size: 496 6429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4375 5136 6164 1540
Size: 275 186 2374 761 1028 2301
Fragments arranged by size:
2374 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4986 5071 6142 4986
Size: 85 1071 5769
Fragments arranged by size:
5769 1071 85
BsmI GAATG_Cn'
Cuts at: 2713 3235 4436 4529 2713
Size: 522 1201 93 5109
Fragments arranged by size:
5109 1201 522 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4244 4986 5071 6142 1391
Size: 399 2454 742 85 1071 2174
Fragments arranged by size:
2454 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 2422 3658 4814 6544 2422
Size: 1236 1156 1730 2803
Fragments arranged by size:
2803 1730 1236 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4280 844
Size: 3436 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4812 1394
Size: 429 2989 3507
Fragments arranged by size:
3507 2989 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5420 5594 65
Size: 1183 4172 174 1396
Fragments arranged by size:
4172 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5569 5756 1700
Size: 110 3759 187 2869
Fragments arranged by size:
3759 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3325 3409 96
Size: 3229 84 3612
Fragments arranged by size:
3612 3229 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 4985 5997 4985
Size: 1012 5913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6925
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 3184 1184
Size: 2000 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 3456 3456
Size: 6925
BstYI r'GATC_y
Cuts at: 1972 2300 2635 4255 4622 5007 5024 6476 6562
Size: 328 335 1620 367 385 17 1452 86
Cuts at: 6562 6574 6920 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1620 1452 385 367 346 335 328
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3180 3180
Size: 6925
BtgI C'CryG_G
Cuts at: 513 1065 2004 3748 3904 513
Size: 552 939 1744 156 3534
Fragments arranged by size:
3534 1744 939 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6186
Fragments arranged by size:
6186 683 56
BtsI GCAGTG_nn'
Cuts at: 4441 5235 5255 4441
Size: 794 20 6111
Fragments arranged by size:
6111 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6925
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6890
DraI TTT'AAA
Cuts at: 4229 4229
Size: 6925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4372 5261 6890
Size: 54 1425 50 275 18 2542 889 1629
Cuts at: 6890 8
Size: 43
Fragments arranged by size:
2542 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4372 1537
Size: 275 2560 4090
Fragments arranged by size:
4090 2560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2730 3820 4855 1353
Size: 699 678 1090 1035 3423
Fragments arranged by size:
3423 1090 1035 699 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5512 5886 6032 387
Size: 1064 138 3923 374 146 1280
Fragments arranged by size:
3923 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 3191 3483 4988 5585 6372 6640
Size: 393 612 947 292 1505 597 787 268
Cuts at: 6640 1239
Size: 1524
Fragments arranged by size:
1524 1505 947 787 612 597 393 292
268
EcoNI CCTnn'n_nnAGG
Cuts at: 4160 4160
Size: 6925
EcoO109I rG'GnC_Cy
Cuts at: 3599 3599
Size: 6925
EcoRI G'AATT_C
Cuts at: 2558 4234 4612 2558
Size: 1676 378 4871
Fragments arranged by size:
4871 1676 378
EcoRV GAT'ATC
Cuts at: 2086 3745 2086
Size: 1659 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3100 3691 3100
Size: 591 6334
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 6072 1678
Size: 4394 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2987 3019 3050
Size: 32 135 32 713 32 670 32 31
Cuts at: 3050 3082 3090 3122 3154 3186 3443 3475 5566
Size: 32 8 32 32 32 257 32 2091
Cuts at: 5566 5598 5640 5672 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2091 713 670 257 135 42 32
32 32 32 32 32 32 32 32
32 32 31 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3292 4269 4515 669
Size: 988 281 1131 223 977 246 3079
Fragments arranged by size:
3079 1131 988 977 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6925
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KpnI G_GTAC'C
Cuts at: 3889 4043 4250 3889
Size: 154 207 6564
Fragments arranged by size:
6564 207 154
MfeI C'AATT_G
Cuts at: 2313 4524 2313
Size: 2211 4714
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6039 6223 1305
Size: 4734 184 2007
Fragments arranged by size:
4734 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5103
Fragments arranged by size:
5103 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 4883 5242 5401 518
Size: 648 1240 2477 359 159 2042
Fragments arranged by size:
2477 2042 1240 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4210 4341 5018 6166 6411 1606
Size: 2604 131 677 1148 245 2120
Fragments arranged by size:
2604 2120 1148 677 245 131
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5434
Fragments arranged by size:
5434 939 552
NdeI CA'TA_TG
Cuts at: 387 2290 2667 3956 387
Size: 1903 377 1289 3356
Fragments arranged by size:
3356 1903 1289 377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NsiI A_TGCA'T
Cuts at: 2571 3276 2571
Size: 705 6220
NspI r_CATG'y
Cuts at: 1132 1229 2612 3416 4283 5828 1132
Size: 97 1383 804 867 1545 2229
Fragments arranged by size:
2229 1545 1383 867 804 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6925
PciI A'CATG_T
Cuts at: 2608 5824 2608
Size: 3216 3709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2762 1108
Size: 1654 5271
PfoI T'CCnGG_A
Cuts at: 3043 3108 3043
Size: 65 6860
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3599 3599
Size: 6925
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 3155 3767 3921 4495 3155
Size: 612 154 574 5585
Fragments arranged by size:
5585 612 574 154
PstI C_TGCA'G
Cuts at: 830 2143 4277 830
Size: 1313 2134 3478
Fragments arranged by size:
3478 2134 1313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 4210 4210
Size: 6925
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SapI GCTCTTCn'nnn_
Cuts at: 3820 3820
Size: 6925
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 3578 5173 6885 3578
Size: 1595 1712 3618
Fragments arranged by size:
3618 1712 1595
SexAI A'CCwGG_T
Cuts at: 3433 3433
Size: 6925
SfcI C'TryA_G
Cuts at: 826 1045 2139 3541 4273 5408 6089 6280 826
Size: 219 1094 1402 732 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 1094 732 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 2891 2993 4252 1872
Size: 1019 102 1259 4545
Fragments arranged by size:
4545 1259 1019 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3090 3135 3932 5029 5930 6192
Size: 197 926 1147 45 797 1097 901 262
Cuts at: 6192 820
Size: 1553
Fragments arranged by size:
1553 1147 1097 926 901 797 262 197
45
SnaBI TAC'GTA
Cuts at: 493 3802 4716 493
Size: 3309 914 2702
Fragments arranged by size:
3309 2702 914
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 2465 1517
Size: 948 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2428 2476 2781 4364 513
Size: 552 939 424 48 305 1583 3074
Fragments arranged by size:
3074 1583 939 552 424 305 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 2355 4952 4969 5122 5307 5752
Size: 1255 435 340 2597 17 153 185 445
Cuts at: 5752 325
Size: 1498
Fragments arranged by size:
2597 1498 1255 445 435 340 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2128 3325 3409
Size: 274 80 33 51 1443 151 1197 84
Cuts at: 3409 3576 5171 6883 96
Size: 167 1595 1712 138
Fragments arranged by size:
1712 1595 1443 1197 274 167 151 138
84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6885 6917 6885
Size: 32 6893
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6925
XmaI C'CCGG_G
Cuts at: 1870 2889 2991 4250 1870
Size: 1019 102 1259 4545
Fragments arranged by size:
4545 1259 1019 102
XmnI GAAnn'nnTTC
Cuts at: 5054 5054
Size: 6925
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4733 276
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI