Restriction map for
pFN21AB5734
(Circular) (Six-base) MAPSORT of: pFN21AB5734.seq Check: 3405 from: 1 to: 6910
pFN21AB5734 6910 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 4720 278
Size: 53 83 186 936 2500 684 2468
Fragments arranged by size:
2500 2468 936 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 4253 1937
Size: 718 1598 4594
Fragments arranged by size:
4594 1598 718
Acc65I G'GTAC_C
Cuts at: 4231 4231
Size: 6910
AclI AA'CG_TT
Cuts at: 1563 5037 5410 1563
Size: 3474 373 3063
Fragments arranged by size:
3474 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2468 2624 3552 4973 6357 6625 1632
Size: 836 156 928 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 928 836 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6713
AflIII A'CryG_T
Cuts at: 1203 2622 2812 5809 6786 1203
Size: 1419 190 2997 977 1327
Fragments arranged by size:
2997 1419 1327 977 190
AgeI A'CCGG_T
Cuts at: 5741 5741
Size: 6910
AhdI GACnn_n'nnGTC
Cuts at: 5639 5639
Size: 6910
AleI CACnn'nnGTG
Cuts at: 1166 2564 2815 1166
Size: 1398 251 5261
Fragments arranged by size:
5261 1398 251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3815 1184
Size: 2631 4279
AlwNI CAG_nnn'CTG
Cuts at: 2701 3448 4653 6225 2701
Size: 747 1205 1572 3386
Fragments arranged by size:
3386 1572 1205 747
ApaI G_GGCC'C
Cuts at: 4172 4172
Size: 6910
ApaLI G'TGCA_C
Cuts at: 4967 6123 4967
Size: 1156 5754
ApoI r'AATT_y
Cuts at: 1493 2351 3079 3683 4219 4445 4597 6706 1493
Size: 858 728 604 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 858 728 604 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5464 160
Size: 872 4432 1606
Fragments arranged by size:
4432 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
AvaI C'yCGr_G
Cuts at: 1870 1943 4235 1870
Size: 73 2292 4545
Fragments arranged by size:
4545 2292 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3924 3957 3924
Size: 33 6877
BamHI G'GATC_C
Cuts at: 2388 4240 4607 2388
Size: 1852 367 4691
Fragments arranged by size:
4691 1852 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2910 4231 5686 618
Size: 325 217 627 1123 1321 1455 1842
Fragments arranged by size:
1842 1455 1321 1123 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3258 4172 4229 1391
Size: 1867 914 57 4072
Fragments arranged by size:
4072 1867 914 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2346 3068 928
Size: 971 447 722 4770
Fragments arranged by size:
4770 971 722 447
BbvCI CC'TCA_GC
Cuts at: 2471 2471
Size: 6910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2723 2757 5101 5135 1903
Size: 34 786 34 2344 34 3678
Fragments arranged by size:
3678 2344 786 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4802 6012 4802
Size: 1210 5700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6910
BfrBI ATG'CAT
Cuts at: 3820 3820
Size: 6910
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5521 136
Size: 107 122 71 631 605 3849 1525
Fragments arranged by size:
3849 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3747 6905 3747
Size: 3158 3752
BlpI GC'TnA_GC
Cuts at: 4327 4327
Size: 6910
Bme1580I G_kGCm'C
Cuts at: 1790 4172 4971 6127 1790
Size: 2382 799 1156 2573
Fragments arranged by size:
2573 2382 1156 799
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6910
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 3006 3542
Size: 416 53 83 399 24 611 978 536
Cuts at: 3542 4641 5599 442
Size: 1099 958 1753
Fragments arranged by size:
1753 1099 978 958 611 536 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 2612 3746 4001 5570 1239
Size: 1103 270 1134 255 1569 2579
Fragments arranged by size:
2579 1569 1134 1103 270 255
Bpu10I CC'TnA_GC
Cuts at: 2213 2471 2213
Size: 258 6652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2517 3446 4004 4225 5035 5900 6198 2517
Size: 929 558 221 810 865 298 3229
Fragments arranged by size:
3229 929 865 810 558 298 221
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3326 3847 5573 882
Size: 617 1827 521 1726 2219
Fragments arranged by size:
2219 1827 1726 617 521
BsaAI yAC'GTr
Cuts at: 493 1204 4701 493
Size: 711 3497 2702
Fragments arranged by size:
3497 2702 711
BsaBI GATnn'nnATC
Cuts at: 2873 6904 2873
Size: 4031 2879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4033 4118 4717 5099
Size: 53 83 186 936 2500 85 599 382
Cuts at: 5099 5734 275
Size: 635 1451
Fragments arranged by size:
2500 1451 936 635 599 382 186 85
83 53
BsaWI w'CCGG_w
Cuts at: 2871 3031 3637 5343 5741 6015 6162 6573 2871
Size: 160 606 1706 398 274 147 411 3208
Fragments arranged by size:
3208 1706 606 411 398 274 160 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6685
Fragments arranged by size:
6685 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2841 3441 3743 1186
Size: 1655 600 302 4353
Fragments arranged by size:
4353 1655 600 302
BseYI C'CCAG_C
Cuts at: 2046 2552 2584 6113 6609 2046
Size: 506 32 3529 496 2347
Fragments arranged by size:
3529 2347 506 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 4360 5121 6149 1540
Size: 275 186 853 1506 761 1028 2301
Fragments arranged by size:
2301 1506 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3089 3186 4052 4971 5056 6127 3089
Size: 97 866 919 85 1071 3872
Fragments arranged by size:
3872 1071 919 866 97 85
BsmI GAATG_Cn'
Cuts at: 4030 4421 4514 4030
Size: 391 93 6426
Fragments arranged by size:
6426 391 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3300 3300
Size: 6910
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3089 3186 3258 4052 4172 4229 4971
Size: 399 1299 97 72 794 120 57 742
Cuts at: 4971 5056 6127 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1299 1071 794 742 399 120 97
85 72 57
BspEI T'CCGG_A
Cuts at: 2871 3637 6573 2871
Size: 766 2936 3208
Fragments arranged by size:
3208 2936 766
BspHI T'CATG_A
Cuts at: 4799 6529 4799
Size: 1730 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4265 844
Size: 3421 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 4797 1394
Size: 429 2359 615 3507
Fragments arranged by size:
3507 2359 615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5405 5579 65
Size: 1183 4157 174 1396
Fragments arranged by size:
4157 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5554 5741 1700
Size: 110 3744 187 2869
Fragments arranged by size:
3744 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4124 96
Size: 4028 2882
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6910
BssSI C'ACGA_G
Cuts at: 4970 5982 4970
Size: 1012 5898
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2667 3181 794
Size: 1873 514 4523
Fragments arranged by size:
4523 1873 514
BstBI TT'CG_AA
Cuts at: 3681 5714 3681
Size: 2033 4877
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6910
BstXI CCAn_nnnn'nTGG
Cuts at: 3019 4165 3019
Size: 1146 5764
BstYI r'GATC_y
Cuts at: 1972 2388 2868 2874 2922 3456 3747 3766 4240
Size: 416 480 6 48 534 291 19 474
Cuts at: 4240 4607 4992 5009 6461 6547 6559 6905 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 534 480 474 416 385 367
346 291 86 48 19 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 3889 3889
Size: 6910
BtgI C'CryG_G
Cuts at: 513 1065 2004 3066 3859 513
Size: 552 939 1062 793 3564
Fragments arranged by size:
3564 1062 939 793 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3778 487
Size: 683 56 2552 3619
Fragments arranged by size:
3619 2552 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 2524 2570 3804 4426 5220 5240 2248
Size: 276 46 1234 622 794 20 3918
Fragments arranged by size:
3918 1234 794 622 276 46 20
ClaI AT'CG_AT
Cuts at: 1554 3499 3610 1554
Size: 1945 111 4854
Fragments arranged by size:
4854 1945 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2262 2297 2522 2557 529
Size: 35 1698 35 225 35 4882
Fragments arranged by size:
4882 1698 225 35 35 35
DraI TTT'AAA
Cuts at: 3078 4214 3078
Size: 1136 5774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 5917 809
Size: 507 1485 3116 1802
Fragments arranged by size:
3116 1802 1485 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2333 4041
Size: 54 1425 50 275 18 177 326 1708
Cuts at: 4041 4357 5246 6875 8
Size: 316 889 1629 43
Fragments arranged by size:
1708 1629 1425 889 326 316 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4357 1537
Size: 275 2545 4090
Fragments arranged by size:
4090 2545 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 4840 1353
Size: 755 2732 3423
Fragments arranged by size:
3423 2732 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5497 5871 6017 387
Size: 1064 138 3908 374 146 1280
Fragments arranged by size:
3908 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2468 2612 2624 3552 3746 4001
Size: 393 710 126 144 12 928 194 255
Cuts at: 4001 4973 5570 6357 6625 1239
Size: 972 597 787 268 1524
Fragments arranged by size:
1524 972 928 787 710 597 393 268
255 194 144 126 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 3233 3249 2321
Size: 912 16 5982
Fragments arranged by size:
5982 912 16
EcoO109I rG'GnC_Cy
Cuts at: 2020 2020
Size: 6910
EcoRI G'AATT_C
Cuts at: 2351 4219 4597 2351
Size: 1868 378 4664
Fragments arranged by size:
4664 1868 378
EcoRV GAT'ATC
Cuts at: 3868 3925 3868
Size: 57 6853
FspI TGC'GCA
Cuts at: 5416 5416
Size: 6910
HaeII r_GCGC'y
Cuts at: 1678 2848 6057 1678
Size: 1170 3209 2531
Fragments arranged by size:
3209 2531 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3488 3520 3603 3635 5551
Size: 32 135 32 1916 32 83 32 1916
Cuts at: 5551 5583 5625 5657 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1916 1916 135 83 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4254 4500 669
Size: 988 281 2316 246 3079
Fragments arranged by size:
3079 2316 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6910
HpaI GTT'AAC
Cuts at: 4500 4500
Size: 6910
KpnI G_GTAC'C
Cuts at: 4235 4235
Size: 6910
MfeI C'AATT_G
Cuts at: 3990 4509 3990
Size: 519 6391
MluI A'CGCG_T
Cuts at: 6786 6786
Size: 6910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3855 6024 6208 1305
Size: 2550 2169 184 2007
Fragments arranged by size:
2550 2169 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 4043 10
Size: 54 1425 343 177 2034 2877
Fragments arranged by size:
2877 2034 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 2815 2958 3797 4163 4868 5227
Size: 648 1398 251 143 839 366 705 359
Cuts at: 5227 5386 518
Size: 159 2042
Fragments arranged by size:
2042 1398 839 705 648 366 359 251
159 143
MspA1I CmG'CkG
Cuts at: 1606 2050 2856 3763 4326 5003 6151 6396 1606
Size: 444 806 907 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 806 677 563 444 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3066 3859 513
Size: 552 939 1062 793 3564
Fragments arranged by size:
3564 1062 939 793 552
NdeI CA'TA_TG
Cuts at: 387 3492 387
Size: 3105 3805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6910
NotI GC'GGCC_GC
Cuts at: 4357 4357
Size: 6910
NsiI A_TGCA'T
Cuts at: 3822 3822
Size: 6910
NspI r_CATG'y
Cuts at: 1132 1229 2626 2816 3717 3820 4268 5813 1132
Size: 97 1397 190 901 103 448 1545 2229
Fragments arranged by size:
2229 1545 1397 901 448 190 103 97
PasI CC'CwG_GG
Cuts at: 1348 3385 1348
Size: 2037 4873
PciI A'CATG_T
Cuts at: 2622 2812 5809 2622
Size: 190 2997 3723
Fragments arranged by size:
3723 2997 190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3837 1108
Size: 2729 4181
PfoI T'CCnGG_A
Cuts at: 2391 2976 2391
Size: 585 6325
PmeI GTTT'AAAC
Cuts at: 4214 4214
Size: 6910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 6492 6524 4979
Size: 32 1481 32 5365
Fragments arranged by size:
5365 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2856 1613
Size: 1243 5667
PsiI TTA'TAA
Cuts at: 4480 4480
Size: 6910
PspOMI G'GGCC_C
Cuts at: 4168 4168
Size: 6910
PstI C_TGCA'G
Cuts at: 830 4262 830
Size: 3432 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6910
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 6910
SalI G'TCGA_C
Cuts at: 1936 4252 1936
Size: 2316 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2108
Size: 6910
SbfI CC_TGCA'GG
Cuts at: 4262 4262
Size: 6910
ScaI AGT'ACT
Cuts at: 5158 6870 5158
Size: 1712 5198
SfcI C'TryA_G
Cuts at: 826 1045 2423 3897 4258 5393 6074 6265 826
Size: 219 1378 1474 361 1135 681 191 1471
Fragments arranged by size:
1474 1471 1378 1135 681 361 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6910
SmaI CCC'GGG
Cuts at: 1872 4237 1872
Size: 2365 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2532 3461 4019 4204 5014 5915
Size: 197 926 589 929 558 185 810 901
Cuts at: 5915 6177 820
Size: 262 1553
Fragments arranged by size:
1553 929 926 901 810 589 558 262
197 185
SnaBI TAC'GTA
Cuts at: 493 4701 493
Size: 4208 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6910
SphI G_CATG'C
Cuts at: 1132 3820 4268 1132
Size: 2688 448 3774
Fragments arranged by size:
3774 2688 448
SspI AAT'ATT
Cuts at: 5 52 4834 5
Size: 47 4782 2081
Fragments arranged by size:
4782 2081 47
StuI AGG'CCT
Cuts at: 1517 3887 1517
Size: 2370 4540
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2134 3066 3859 4349 513
Size: 552 939 6 124 932 793 490 3074
Fragments arranged by size:
3074 939 932 793 552 490 124 6
SwaI ATTT'AAAT
Cuts at: 3078 3078
Size: 6910
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2809 3140 3144 4154 4937 4954 5107
Size: 1255 1229 331 4 1010 783 17 153
Cuts at: 5107 5292 5737 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1229 1010 783 445 331 185
153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 4124 5156
Size: 274 80 33 51 1443 744 1403 1032
Cuts at: 5156 6868 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1403 1032 744 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6870 6902 6870
Size: 32 6878
Tth111I GACn'n_nGTC
Cuts at: 1316 3548 1316
Size: 2232 4678
XbaI T'CTAG_A
Cuts at: 3328 4246 3328
Size: 918 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6910
XmaI C'CCGG_G
Cuts at: 1870 4235 1870
Size: 2365 4545
XmnI GAAnn'nnTTC
Cuts at: 5039 5039
Size: 6910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 4718 276
Size: 53 83 186 936 2500 684 2468
Fragments arranged by size:
2500 2468 936 684 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
XcmI