Restriction map for
pFN21AB5718
(Circular) (Six-base) MAPSORT of: pFN21AB5718.seq Check: 3038 from: 1 to: 6877
pFN21AB5718 6877 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4687 278
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4220 1937
Size: 2283 4594
Acc65I G'GTAC_C
Cuts at: 4198 4198
Size: 6877
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2745 2859 3478 4940 6324 6592 1632
Size: 1113 114 619 1462 1384 268 1917
Fragments arranged by size:
1917 1462 1384 1113 619 268 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 3080 5776 6753 1203
Size: 1877 2696 977 1327
Fragments arranged by size:
2696 1877 1327 977
AgeI A'CCGG_T
Cuts at: 5708 5708
Size: 6877
AhdI GACnn_n'nnGTC
Cuts at: 5606 5606
Size: 6877
AleI CACnn'nnGTG
Cuts at: 1166 3846 1166
Size: 2680 4197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6877
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3453 3485 3453
Size: 32 6845
AlwNI CAG_nnn'CTG
Cuts at: 3110 3533 4620 6192 3110
Size: 423 1087 1572 3795
Fragments arranged by size:
3795 1572 1087 423
ApaLI G'TGCA_C
Cuts at: 4934 6090 4934
Size: 1156 5721
ApoI r'AATT_y
Cuts at: 1493 2191 3603 4186 4412 4564 6673 1493
Size: 698 1412 583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 698 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5431 160
Size: 872 4399 1606
Fragments arranged by size:
4399 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 3768 4113 4202 1870
Size: 73 1825 345 89 4545
Fragments arranged by size:
4545 1825 345 89 73
BamHI G'GATC_C
Cuts at: 2539 4207 4574 2539
Size: 1668 367 4842
Fragments arranged by size:
4842 1668 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4198 5653 618
Size: 325 217 627 2411 1455 1842
Fragments arranged by size:
2411 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4196 1391
Size: 2805 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3897 4097 928
Size: 971 1998 200 3708
Fragments arranged by size:
3708 1998 971 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3126 3160 5068 5102 1903
Size: 34 1189 34 1908 34 3678
Fragments arranged by size:
3678 1908 1189 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3098 3137 4769 5979 3098
Size: 39 1632 1210 3996
Fragments arranged by size:
3996 1632 1210 39
BclI T'GATC_A
Cuts at: 1548 2155 3942 1548
Size: 607 1787 4483
Fragments arranged by size:
4483 1787 607
BfrBI ATG'CAT
Cuts at: 2078 2078
Size: 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5488 136
Size: 107 122 71 631 605 3816 1525
Fragments arranged by size:
3816 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3251 3743 3979 6872 3251
Size: 492 236 2893 3256
Fragments arranged by size:
3256 2893 492 236
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2752 4938 6094 1790
Size: 962 2186 1156 2573
Fragments arranged by size:
2573 2186 1156 962
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3731 4608 5566
Size: 416 53 83 399 24 2314 877 958
Cuts at: 5566 442
Size: 1753
Fragments arranged by size:
2314 1753 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3783 3783
Size: 6877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2755 3417 5537 1239
Size: 903 613 662 2120 2579
Fragments arranged by size:
2579 2120 903 662 613
Bpu10I CC'TnA_GC
Cuts at: 4036 4036
Size: 6877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2529 3195 3557 5002 5867 6165 2529
Size: 666 362 1445 865 298 3241
Fragments arranged by size:
3241 1445 865 666 362 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5540 882
Size: 617 4041 2219
Fragments arranged by size:
4041 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2747 3155 4016 4668 493
Size: 711 1543 408 861 652 2702
Fragments arranged by size:
2702 1543 861 711 652 408
BsaBI GATnn'nnATC
Cuts at: 6871 6871
Size: 6877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4684 5066 5701 275
Size: 53 83 186 936 3151 382 635 1451
Fragments arranged by size:
3151 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2030 2277 5310 5708 5982 6129 6540 2030
Size: 247 3033 398 274 147 411 2367
Fragments arranged by size:
3033 2367 411 398 274 247 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 2464 2494 1178
Size: 30 165 30 645 30 386 30 5561
Fragments arranged by size:
5561 645 386 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2321 2324 2472 3465 1186
Size: 915 220 3 148 993 4598
Fragments arranged by size:
4598 993 915 220 148 3
BseYI C'CCAG_C
Cuts at: 6080 6576 6080
Size: 496 6381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4327 5088 6116 1540
Size: 275 186 2326 761 1028 2301
Fragments arranged by size:
2326 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4938 5023 6094 4938
Size: 85 1071 5721
Fragments arranged by size:
5721 1071 85
BsmI GAATG_Cn'
Cuts at: 2529 3468 4388 4481 2529
Size: 939 920 93 4925
Fragments arranged by size:
4925 939 920 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 4196 4938 5023 6094 1391
Size: 399 962 1444 742 85 1071 2174
Fragments arranged by size:
2174 1444 1071 962 742 399 85
BspEI T'CCGG_A
Cuts at: 2030 6540 2030
Size: 4510 2367
BspHI T'CATG_A
Cuts at: 3058 4766 6496 3058
Size: 1708 1730 3439
Fragments arranged by size:
3439 1730 1708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2793 4232 844
Size: 1949 1439 3489
Fragments arranged by size:
3489 1949 1439
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 4764 1394
Size: 429 187 2754 3507
Fragments arranged by size:
3507 2754 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2650 5372 5546 65
Size: 1183 1402 2722 174 1396
Fragments arranged by size:
2722 1402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5521 5708 1700
Size: 110 3711 187 2869
Fragments arranged by size:
3711 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6877
BssSI C'ACGA_G
Cuts at: 3231 4937 5949 3231
Size: 1706 1012 4159
Fragments arranged by size:
4159 1706 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6877
BstBI TT'CG_AA
Cuts at: 5681 5681
Size: 6877
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6877
BstYI r'GATC_y
Cuts at: 1972 2125 2371 2539 3251 3743 3979 4207 4574
Size: 153 246 168 712 492 236 228 367
Cuts at: 4574 4959 4976 6428 6514 6526 6872 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 712 492 385 367 346 246
236 228 168 153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3558 3622 513
Size: 552 2493 64 3768
Fragments arranged by size:
3768 2493 552 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6138
Fragments arranged by size:
6138 683 56
BtsI GCAGTG_nn'
Cuts at: 2113 3838 4036 4051 4393 5187 5207 2113
Size: 1725 198 15 342 794 20 3783
Fragments arranged by size:
3783 1725 794 342 198 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 2190 3183 3286 4181 2190
Size: 993 103 895 4886
Fragments arranged by size:
4886 993 895 103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5884 809
Size: 507 4568 1802
Fragments arranged by size:
4568 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2110 3035 4324
Size: 54 1425 50 275 18 280 925 1289
Cuts at: 4324 5213 6842 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1289 925 889 280 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4324 1537
Size: 275 2512 4090
Fragments arranged by size:
4090 2512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2632 3781 3846 4160 4807 1353
Size: 725 554 1149 65 314 647 3423
Fragments arranged by size:
3423 1149 725 647 554 314 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5464 5838 5984 387
Size: 1064 138 3875 374 146 1280
Fragments arranged by size:
3875 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2745 2755 2859 3417 3478 4940
Size: 393 510 603 10 104 558 61 1462
Cuts at: 4940 5537 6324 6592 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1462 787 603 597 558 510 393
268 104 61 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3135 3135
Size: 6877
EcoRI G'AATT_C
Cuts at: 4186 4564 4186
Size: 378 6499
EcoRV GAT'ATC
Cuts at: 2263 2263
Size: 6877
FspI TGC'GCA
Cuts at: 5383 5383
Size: 6877
HaeII r_GCGC'y
Cuts at: 1678 3692 6024 1678
Size: 2014 2332 2531
Fragments arranged by size:
2531 2332 2014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 3437 3469 3735
Size: 32 135 32 878 32 955 32 266
Cuts at: 3735 3767 4084 4116 5518 5550 5592 5624 1373
Size: 32 317 32 1402 32 42 32 2626
Fragments arranged by size:
2626 1402 955 878 317 266 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2201 2767 3303 4081 4221 4467
Size: 988 281 263 566 536 778 140 246
Cuts at: 4467 669
Size: 3079
Fragments arranged by size:
3079 988 778 566 536 281 263 246
140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6877
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KpnI G_GTAC'C
Cuts at: 4202 4202
Size: 6877
MfeI C'AATT_G
Cuts at: 4476 4476
Size: 6877
MluI A'CGCG_T
Cuts at: 6753 6753
Size: 6877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2384 2960 5991 6175 1305
Size: 1079 576 3031 184 2007
Fragments arranged by size:
3031 2007 1079 576 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5055
Fragments arranged by size:
5055 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3846 4835 5194 5353 518
Size: 648 2680 989 359 159 2042
Fragments arranged by size:
2680 2042 989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2411 4293 4970 6118 6363 1606
Size: 805 1882 677 1148 245 2120
Fragments arranged by size:
2120 1882 1148 805 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3622 513
Size: 552 2557 3768
Fragments arranged by size:
3768 2557 552
NdeI CA'TA_TG
Cuts at: 387 3486 387
Size: 3099 3778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6877
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NsiI A_TGCA'T
Cuts at: 2080 2080
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 2606 2912 4235 5780 1132
Size: 97 1377 306 1323 1545 2229
Fragments arranged by size:
2229 1545 1377 1323 306 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 5776 5776
Size: 6877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2942 1108
Size: 1834 5043
PfoI T'CCnGG_A
Cuts at: 3433 3433
Size: 6877
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PmlI CAC'GTG
Cuts at: 2747 2747
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4946 4978 6459 6491 4946
Size: 32 1481 32 5332
Fragments arranged by size:
5332 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6877
PsiI TTA'TAA
Cuts at: 2781 4447 2781
Size: 1666 5211
PstI C_TGCA'G
Cuts at: 830 4229 830
Size: 3399 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
PvuII CAG'CTG
Cuts at: 2411 2411
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 4219 1936
Size: 2283 4594
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 2836 5125 6837 2836
Size: 2289 1712 2876
Fragments arranged by size:
2876 2289 1712
SexAI A'CCwGG_T
Cuts at: 2941 2941
Size: 6877
SfcI C'TryA_G
Cuts at: 826 1045 3132 4225 5360 6041 6232 826
Size: 219 2087 1093 1135 681 191 1471
Fragments arranged by size:
2087 1471 1135 1093 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 4115 4204 1872
Size: 2243 89 4545
Fragments arranged by size:
4545 2243 89
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2544 3210 3572 3768 4981 5882
Size: 197 926 601 666 362 196 1213 901
Cuts at: 5882 6144 820
Size: 262 1553
Fragments arranged by size:
1553 1213 926 901 666 601 362 262
197 196
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 2606 4235 1132
Size: 1474 1629 3774
Fragments arranged by size:
3774 1629 1474
SspI AAT'ATT
Cuts at: 5 52 2144 4801 5
Size: 47 2092 2657 2081
Fragments arranged by size:
2657 2092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6877
StyI C'CwwG_G
Cuts at: 513 1065 2985 3622 4095 4316 513
Size: 552 1920 637 473 221 3074
Fragments arranged by size:
3074 1920 637 552 473 221
SwaI ATTT'AAAT
Cuts at: 2190 2190
Size: 6877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3078 4904 4921 5074 5259 5704
Size: 1255 885 613 1826 17 153 185 445
Cuts at: 5704 325
Size: 1498
Fragments arranged by size:
1826 1498 1255 885 613 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2834 5123 6835
Size: 274 80 33 51 1443 857 2289 1712
Cuts at: 6835 96
Size: 138
Fragments arranged by size:
2289 1712 1443 857 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 6837 6869 2872
Size: 32 3933 32 2880
Fragments arranged by size:
3933 2880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6877
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2690 3172 2690
Size: 482 6395
XhoI C'TCGA_G
Cuts at: 1943 3768 1943
Size: 1825 5052
XmaI C'CCGG_G
Cuts at: 1870 4113 4202 1870
Size: 2243 89 4545
Fragments arranged by size:
4545 2243 89
XmnI GAAnn'nnTTC
Cuts at: 3316 3402 3640 5006 3316
Size: 86 238 1366 5187
Fragments arranged by size:
5187 1366 238 86
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4685 276
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI