Restriction map for
pFN21AB5714
(Circular) (Six-base) MAPSORT of: pFN21AB5714.seq Check: 2368 from: 1 to: 6868
pFN21AB5714 6868 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3642 3642
Size: 6868
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4678 278
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 4211 1937
Size: 382 1892 4594
Fragments arranged by size:
4594 1892 382
Acc65I G'GTAC_C
Cuts at: 4189 4189
Size: 6868
AclI AA'CG_TT
Cuts at: 1563 4995 5368 1563
Size: 3432 373 3063
Fragments arranged by size:
3432 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4931 6315 6583 1632
Size: 3299 1384 268 1917
Fragments arranged by size:
3299 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6671
AflIII A'CryG_T
Cuts at: 1203 5767 6744 1203
Size: 4564 977 1327
Fragments arranged by size:
4564 1327 977
AgeI A'CCGG_T
Cuts at: 5699 5699
Size: 6868
AhdI GACnn_n'nnGTC
Cuts at: 5597 5597
Size: 6868
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6868
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2178 1184
Size: 994 5874
AlwNI CAG_nnn'CTG
Cuts at: 3013 3046 4611 6183 3013
Size: 33 1565 1572 3698
Fragments arranged by size:
3698 1572 1565 33
ApaLI G'TGCA_C
Cuts at: 4925 6081 4925
Size: 1156 5712
ApoI r'AATT_y
Cuts at: 1493 3267 3981 4177 4403 4555 6664 1493
Size: 1774 714 196 226 152 2109 1697
Fragments arranged by size:
2109 1774 1697 714 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 5422 160
Size: 872 4390 1606
Fragments arranged by size:
4390 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
AvaI C'yCGr_G
Cuts at: 1870 1943 4193 1870
Size: 73 2250 4545
Fragments arranged by size:
4545 2250 73
BamHI G'GATC_C
Cuts at: 4198 4565 4198
Size: 367 6501
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4189 5644 618
Size: 325 217 627 2402 1455 1842
Fragments arranged by size:
2402 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2186 4187 1391
Size: 795 2001 4072
Fragments arranged by size:
4072 2001 795
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2167 3576 3744 928
Size: 971 268 1409 168 4052
Fragments arranged by size:
4052 1409 971 268 168
BbvCI CC'TCA_GC
Cuts at: 3888 3888
Size: 6868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5059 5093 1903
Size: 34 50 34 3038 34 3678
Fragments arranged by size:
3678 3038 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4760 5970 4760
Size: 1210 5658
BclI T'GATC_A
Cuts at: 1548 3850 4086 1548
Size: 2302 236 4330
Fragments arranged by size:
4330 2302 236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5479 136
Size: 107 122 71 631 605 3807 1525
Fragments arranged by size:
3807 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6863 6863
Size: 6868
BlpI GC'TnA_GC
Cuts at: 4285 4285
Size: 6868
Bme1580I G_kGCm'C
Cuts at: 1790 3526 4929 6085 1790
Size: 1736 1403 1156 2573
Fragments arranged by size:
2573 1736 1403 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6868
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2841 3400 4599
Size: 416 53 83 399 24 1424 559 1199
Cuts at: 4599 5557 442
Size: 958 1753
Fragments arranged by size:
1753 1424 1199 958 559 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2502 2731 3301 5528 1239
Size: 1263 229 570 2227 2579
Fragments arranged by size:
2579 2227 1263 570 229
Bpu10I CC'TnA_GC
Cuts at: 2259 3888 2259
Size: 1629 5239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4993 5858 6156 4993
Size: 865 298 5705
Fragments arranged by size:
5705 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3179 5531 882
Size: 617 1680 2352 2219
Fragments arranged by size:
2352 2219 1680 617
BsaAI yAC'GTr
Cuts at: 493 1204 3289 4659 493
Size: 711 2085 1370 2702
Fragments arranged by size:
2702 2085 1370 711
BsaBI GATnn'nnATC
Cuts at: 3120 3810 6862 3120
Size: 690 3052 3126
Fragments arranged by size:
3126 3052 690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4675 5057 5692 275
Size: 53 83 186 936 3142 382 635 1451
Fragments arranged by size:
3142 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2895 5301 5699 5973 6120 6531 2895
Size: 2406 398 274 147 411 3232
Fragments arranged by size:
3232 2406 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 3299 3329 1178
Size: 30 165 30 1072 30 794 30 4717
Fragments arranged by size:
4717 1072 794 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6868
BseYI C'CCAG_C
Cuts at: 2377 3136 3299 6071 6567 2377
Size: 759 163 2772 496 2678
Fragments arranged by size:
2772 2678 759 496 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3021 3353 1163
Size: 573 1285 332 4678
Fragments arranged by size:
4678 1285 573 332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 4318 5079 6107 1540
Size: 275 186 102 2215 761 1028 2301
Fragments arranged by size:
2301 2215 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 2434 4929 5014 6085 2434
Size: 2495 85 1071 3217
Fragments arranged by size:
3217 2495 1071 85
BsmI GAATG_Cn'
Cuts at: 2742 3303 3380 3592 4379 4472 2742
Size: 561 77 212 787 93 5138
Fragments arranged by size:
5138 787 561 212 93 77
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2434 3526 4187 4929 5014 6085
Size: 399 396 248 1092 661 742 85 1071
Cuts at: 6085 1391
Size: 2174
Fragments arranged by size:
2174 1092 1071 742 661 399 396 248
85
BspEI T'CCGG_A
Cuts at: 6531 6531
Size: 6868
BspHI T'CATG_A
Cuts at: 2449 4757 6487 2449
Size: 2308 1730 2830
Fragments arranged by size:
2830 2308 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2964 3642 4040 4223 844
Size: 2120 678 398 183 3489
Fragments arranged by size:
3489 2120 678 398 183
BsrBI CCG'CTC
Cuts at: 1394 1823 4755 1394
Size: 429 2932 3507
Fragments arranged by size:
3507 2932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3510 5363 5537 65
Size: 1183 2262 1853 174 1396
Fragments arranged by size:
2262 1853 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2873 5512 5699 1700
Size: 110 1063 2639 187 2869
Fragments arranged by size:
2869 2639 1063 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6868
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6868
BssSI C'ACGA_G
Cuts at: 4928 5940 4928
Size: 1012 5856
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6868
BstBI TT'CG_AA
Cuts at: 4111 5672 4111
Size: 1561 5307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6868
BstYI r'GATC_y
Cuts at: 1972 2549 3364 4198 4565 4950 4967 6419 6505
Size: 577 815 834 367 385 17 1452 86
Cuts at: 6505 6517 6863 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 834 815 577 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6129
Fragments arranged by size:
6129 683 56
BtsI GCAGTG_nn'
Cuts at: 4089 4384 5178 5198 4089
Size: 295 794 20 5759
Fragments arranged by size:
5759 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6833
DraI TTT'AAA
Cuts at: 4172 4172
Size: 6868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5875 809
Size: 507 4559 1802
Fragments arranged by size:
4559 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2100 2905
Size: 54 1425 50 275 18 231 39 805
Cuts at: 2905 3093 3628 4315 5204 6833 8
Size: 188 535 687 889 1629 43
Fragments arranged by size:
1629 1425 889 805 687 535 275 231
188 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 4315 1537
Size: 275 288 2215 4090
Fragments arranged by size:
4090 2215 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 3920 4798 1353
Size: 858 1709 878 3423
Fragments arranged by size:
3423 1709 878 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5455 5829 5975 387
Size: 1064 138 3866 374 146 1280
Fragments arranged by size:
3866 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2731 3301 4931 5528 6315 6583
Size: 393 870 229 570 1630 597 787 268
Cuts at: 6583 1239
Size: 1524
Fragments arranged by size:
1630 1524 870 787 597 570 393 268
229
EcoO109I rG'GnC_Cy
Cuts at: 3010 3010
Size: 6868
EcoRI G'AATT_C
Cuts at: 4177 4555 4177
Size: 378 6490
EcoRV GAT'ATC
Cuts at: 2566 3703 2566
Size: 1137 5731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 6868
FspI TGC'GCA
Cuts at: 5374 5374
Size: 6868
HaeII r_GCGC'y
Cuts at: 1678 2074 6015 1678
Size: 396 3941 2531
Fragments arranged by size:
3941 2531 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 5509 5541 5583
Size: 32 135 32 1112 32 2793 32 42
Cuts at: 5583 5615 1373
Size: 32 2626
Fragments arranged by size:
2793 2626 1112 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3502 4212 4458 669
Size: 988 281 1564 710 246 3079
Fragments arranged by size:
3079 1564 988 710 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6868
HpaI GTT'AAC
Cuts at: 3502 4458 3502
Size: 956 5912
KpnI G_GTAC'C
Cuts at: 4193 4193
Size: 6868
MfeI C'AATT_G
Cuts at: 2370 2425 2868 4467 2370
Size: 55 443 1599 4771
Fragments arranged by size:
4771 1599 443 55
MluI A'CGCG_T
Cuts at: 6744 6744
Size: 6868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2527 2662 3862 4045 5982 6166 1305
Size: 1222 135 1200 183 1937 184 2007
Fragments arranged by size:
2007 1937 1222 1200 184 183 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 2907 3095 10
Size: 54 1425 343 231 844 188 3783
Fragments arranged by size:
3783 1425 844 343 231 188 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 4148 4826 5185 5344 518
Size: 648 994 1988 678 359 159 2042
Fragments arranged by size:
2042 1988 994 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 4284 4961 6109 6354 1606
Size: 626 2052 677 1148 245 2120
Fragments arranged by size:
2120 2052 1148 677 626 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6316
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6868
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6868
NotI GC'GGCC_GC
Cuts at: 2100 4315 2100
Size: 2215 4653
NspI r_CATG'y
Cuts at: 1132 1229 2159 4226 5771 1132
Size: 97 930 2067 1545 2229
Fragments arranged by size:
2229 2067 1545 930 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6868
PciI A'CATG_T
Cuts at: 5767 5767
Size: 6868
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3715 1108
Size: 2607 4261
PmeI GTTT'AAAC
Cuts at: 4172 4172
Size: 6868
PmlI CAC'GTG
Cuts at: 3289 3289
Size: 6868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4937 4969 6450 6482 4937
Size: 32 1481 32 5323
Fragments arranged by size:
5323 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3010 3010
Size: 6868
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6868
PsiI TTA'TAA
Cuts at: 4438 4438
Size: 6868
PstI C_TGCA'G
Cuts at: 830 2245 4220 830
Size: 1415 1975 3478
Fragments arranged by size:
3478 1975 1415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6868
PvuII CAG'CTG
Cuts at: 2232 2232
Size: 6868
SalI G'TCGA_C
Cuts at: 1936 4210 1936
Size: 2274 4594
SbfI CC_TGCA'GG
Cuts at: 4220 4220
Size: 6868
ScaI AGT'ACT
Cuts at: 5116 6828 5116
Size: 1712 5156
SexAI A'CCwGG_T
Cuts at: 4080 4080
Size: 6868
SfcI C'TryA_G
Cuts at: 826 1045 2241 2341 4216 5351 6032 6223 826
Size: 219 1196 100 1875 1135 681 191 1471
Fragments arranged by size:
1875 1471 1196 1135 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6868
SmaI CCC'GGG
Cuts at: 1872 4195 1872
Size: 2323 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4972 5873 6135 820
Size: 197 926 3029 901 262 1553
Fragments arranged by size:
3029 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4659 493
Size: 4166 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6868
SphI G_CATG'C
Cuts at: 1132 4226 1132
Size: 3094 3774
SspI AAT'ATT
Cuts at: 5 52 4792 5
Size: 47 4740 2081
Fragments arranged by size:
4740 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6868
StyI C'CwwG_G
Cuts at: 513 1065 2499 3081 4307 513
Size: 552 1434 582 1226 3074
Fragments arranged by size:
3074 1434 1226 582 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2841 3419 3536 4895 4912 5065
Size: 1255 543 718 578 117 1359 17 153
Cuts at: 5065 5250 5695 325
Size: 185 445 1498
Fragments arranged by size:
1498 1359 1255 718 578 543 445 185
153 117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5114 6826 96
Size: 274 80 33 51 1443 3137 1712 138
Fragments arranged by size:
3137 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 6828 6860 3299
Size: 32 3497 32 3307
Fragments arranged by size:
3497 3307 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6868
XbaI T'CTAG_A
Cuts at: 4204 4204
Size: 6868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3088 3088
Size: 6868
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6868
XmaI C'CCGG_G
Cuts at: 1870 4193 1870
Size: 2323 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 3741 4997 3380
Size: 361 1256 5251
Fragments arranged by size:
5251 1256 361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4676 276
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI