Restriction map for
pFN21AB5713
(Circular) (Six-base) MAPSORT of: pFN21AB5713.seq Check: 1263 from: 1 to: 7060
pFN21AB5713 7060 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4870 278
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2799 3865 4403 1937
Size: 862 1066 538 4594
Fragments arranged by size:
4594 1066 862 538
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2499 3465 4276 5123 6507 6775 1632
Size: 822 45 966 811 847 1384 268 1917
Fragments arranged by size:
1917 1384 966 847 822 811 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6863
AflIII A'CryG_T
Cuts at: 1203 2152 5959 6936 1203
Size: 949 3807 977 1327
Fragments arranged by size:
3807 1327 977 949
AgeI A'CCGG_T
Cuts at: 5891 5891
Size: 7060
AhdI GACnn_n'nnGTC
Cuts at: 5789 5789
Size: 7060
AleI CACnn'nnGTG
Cuts at: 1166 4229 1166
Size: 3063 3997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2881 1184
Size: 1697 5363
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4032 4064 4032
Size: 32 7028
AlwNI CAG_nnn'CTG
Cuts at: 2305 4017 4803 6375 2305
Size: 1712 786 1572 2990
Fragments arranged by size:
2990 1712 1572 786
ApaLI G'TGCA_C
Cuts at: 4232 5117 6273 4232
Size: 885 1156 5019
Fragments arranged by size:
5019 1156 885
ApoI r'AATT_y
Cuts at: 1493 2194 2616 2690 2994 3059 4103 4331 4369
Size: 701 422 74 304 65 1044 228 38
Cuts at: 4369 4595 4747 6856 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 701 422 304 228 226
152 74 65 38
AseI AT'TA_AT
Cuts at: 160 1032 5614 160
Size: 872 4582 1606
Fragments arranged by size:
4582 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 4385 1870
Size: 73 2442 4545
Fragments arranged by size:
4545 2442 73
BamHI G'GATC_C
Cuts at: 4390 4757 4390
Size: 367 6693
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4381 5836 618
Size: 325 217 627 2594 1455 1842
Fragments arranged by size:
2594 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2128 3982 4379 1391
Size: 651 86 1854 397 4072
Fragments arranged by size:
4072 1854 651 397 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 2487 3207 3332 3453 3735 3934
Size: 971 543 45 720 125 121 282 199
Cuts at: 3934 3950 928
Size: 16 4038
Fragments arranged by size:
4038 971 720 543 282 199 125 121
45 16
BbvCI CC'TCA_GC
Cuts at: 2115 3354 2115
Size: 1239 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5251 5285 1903
Size: 34 50 34 3230 34 3678
Fragments arranged by size:
3678 3230 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4952 6162 4952
Size: 1210 5850
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7060
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5671 136
Size: 107 122 71 631 605 3999 1525
Fragments arranged by size:
3999 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2412 7055 2412
Size: 4643 2417
BlpI GC'TnA_GC
Cuts at: 4477 4477
Size: 7060
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5121 6277 1790
Size: 2446 885 1156 2573
Fragments arranged by size:
2573 2446 1156 885
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3646 3816 4791
Size: 416 53 83 399 24 2229 170 975
Cuts at: 4791 5749 442
Size: 958 1753
Fragments arranged by size:
2229 1753 975 958 416 399 170 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4118 5720 1239
Size: 2879 1602 2579
Fragments arranged by size:
2879 2579 1602
Bpu10I CC'TnA_GC
Cuts at: 2115 3354 2115
Size: 1239 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3885 5185 6050 6348 3170
Size: 715 1300 865 298 3882
Fragments arranged by size:
3882 1300 865 715 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 3398 5723 882
Size: 617 772 1127 2325 2219
Fragments arranged by size:
2325 2219 1127 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 4851 493
Size: 711 3647 2702
Fragments arranged by size:
3647 2702 711
BsaBI GATnn'nnATC
Cuts at: 7054 7054
Size: 7060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4867 5249 5884 275
Size: 53 83 186 936 3334 382 635 1451
Fragments arranged by size:
3334 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5493 5891 6165 6312 6723 5493
Size: 398 274 147 411 5830
Fragments arranged by size:
5830 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3519 3549 4032 4062 1178
Size: 30 165 30 2116 30 483 30 4176
Fragments arranged by size:
4176 2116 483 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3522 3734 1186
Size: 2336 212 4512
Fragments arranged by size:
4512 2336 212
BseYI C'CCAG_C
Cuts at: 2062 3301 6263 6759 2062
Size: 1239 2962 496 2363
Fragments arranged by size:
2962 2363 1239 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3072 3363 1163
Size: 573 1336 291 4860
Fragments arranged by size:
4860 1336 573 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4510 5271 6299 1540
Size: 275 186 2509 761 1028 2301
Fragments arranged by size:
2509 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3982 4236 5121 5206 6277 3982
Size: 254 885 85 1071 4765
Fragments arranged by size:
4765 1071 885 254 85
BsmI GAATG_Cn'
Cuts at: 2870 4571 4664 2870
Size: 1701 93 5266
Fragments arranged by size:
5266 1701 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2128 3982 4236 4379 5121 5206
Size: 399 252 86 1854 254 143 742 85
Cuts at: 5206 6277 1391
Size: 1071 2174
Fragments arranged by size:
2174 1854 1071 742 399 254 252 143
86 85
BspEI T'CCGG_A
Cuts at: 6723 6723
Size: 7060
BspHI T'CATG_A
Cuts at: 3313 3516 4949 6679 3313
Size: 203 1433 1730 3694
Fragments arranged by size:
3694 1730 1433 203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4415 844
Size: 3571 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4947 1394
Size: 429 3124 3507
Fragments arranged by size:
3507 3124 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5555 5729 65
Size: 1183 4307 174 1396
Fragments arranged by size:
4307 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5704 5891 1700
Size: 110 3894 187 2869
Fragments arranged by size:
3894 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4285 96
Size: 4189 2871
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 5120 6132 5120
Size: 1012 6048
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3158 794
Size: 2364 4696
BstBI TT'CG_AA
Cuts at: 3198 5864 3198
Size: 2666 4394
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7060
BstXI CCAn_nnnn'nTGG
Cuts at: 2733 4244 2733
Size: 1511 5549
BstYI r'GATC_y
Cuts at: 1972 2412 4390 4757 5142 5159 6611 6697 6709
Size: 440 1978 367 385 17 1452 86 12
Cuts at: 6709 7055 1972
Size: 346 1977
Fragments arranged by size:
1978 1977 1452 440 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3866 3866
Size: 7060
BtgI C'CryG_G
Cuts at: 513 1065 3952 513
Size: 552 2887 3621
Fragments arranged by size:
3621 2887 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6321
Fragments arranged by size:
6321 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 3439 4576 5370 5390 2604
Size: 835 1137 794 20 4274
Fragments arranged by size:
4274 1137 835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3934 3969 529
Size: 35 3370 35 3620
Fragments arranged by size:
3620 3370 35 35
DraI TTT'AAA
Cuts at: 4108 4364 4108
Size: 256 6804
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6067 809
Size: 507 4751 1802
Fragments arranged by size:
4751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2723 3780 4507
Size: 54 1425 50 275 18 893 1057 727
Cuts at: 4507 5396 7025 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1057 893 889 727 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 2988 3045 3249 3696 4990 1353
Size: 1001 634 57 204 447 1294 3423
Fragments arranged by size:
3423 1294 1001 634 447 204 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5647 6021 6167 387
Size: 1064 138 4058 374 146 1280
Fragments arranged by size:
4058 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3980 3980
Size: 7060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2499 3465 4118 4276 5123 5720
Size: 393 822 45 966 653 158 847 597
Cuts at: 5720 6507 6775 1239
Size: 787 268 1524
Fragments arranged by size:
1524 966 847 822 787 653 597 393
268 158 45
EcoNI CCTnn'n_nnAGG
Cuts at: 4052 4052
Size: 7060
EcoRI G'AATT_C
Cuts at: 4369 4747 4369
Size: 378 6682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 7060
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 2021 2069 6207 1678
Size: 343 48 4138 2531
Fragments arranged by size:
4138 2531 343 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 2397 2429 2461
Size: 32 135 32 781 32 12 32 32
Cuts at: 2461 2963 2995 3251 3283 3519 3551 3976 4008
Size: 502 32 256 32 236 32 425 32
Cuts at: 4008 5701 5733 5775 5807 1373
Size: 1693 32 42 32 2626
Fragments arranged by size:
2626 1693 781 502 425 256 236 135
42 32 32 32 32 32 32 32
32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 4404 4650 669
Size: 988 281 559 1907 246 3079
Fragments arranged by size:
3079 1907 988 559 281 246
HindIII A'AGCT_T
Cuts at: 748 2571 2760 4301 748
Size: 1823 189 1541 3507
Fragments arranged by size:
3507 1823 1541 189
HpaI GTT'AAC
Cuts at: 4650 4650
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 4659 4659
Size: 7060
MluI A'CGCG_T
Cuts at: 6936 6936
Size: 7060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2528 3059 3592 6174 6358 1305
Size: 1223 531 533 2582 184 2007
Fragments arranged by size:
2582 2007 1223 533 531 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3782 10
Size: 54 1425 343 1950 3288
Fragments arranged by size:
3288 1950 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2793 3648 4229 4242 5018 5377 5536
Size: 648 1627 855 581 13 776 359 159
Cuts at: 5536 518
Size: 2042
Fragments arranged by size:
2042 1627 855 776 648 581 359 159
13
MspA1I CmG'CkG
Cuts at: 1606 4476 5153 6301 6546 1606
Size: 2870 677 1148 245 2120
Fragments arranged by size:
2870 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6508
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 2156 4418 5963 1132
Size: 97 927 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 927 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7060
PciI A'CATG_T
Cuts at: 2152 5959 2152
Size: 3807 3253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7060
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4032 4064 5129 5161 6642 6674 4032
Size: 32 1065 32 1481 32 4418
Fragments arranged by size:
4418 1481 1065 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7060
PsiI TTA'TAA
Cuts at: 3827 4281 4630 3827
Size: 454 349 6257
Fragments arranged by size:
6257 454 349
PstI C_TGCA'G
Cuts at: 830 4056 4412 830
Size: 3226 356 3478
Fragments arranged by size:
3478 3226 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
SacI G_AGCT'C
Cuts at: 3982 3982
Size: 7060
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SbfI CC_TGCA'GG
Cuts at: 4056 4412 4056
Size: 356 6704
ScaI AGT'ACT
Cuts at: 2840 5308 7020 2840
Size: 2468 1712 2880
Fragments arranged by size:
2880 2468 1712
SfcI C'TryA_G
Cuts at: 826 1045 2679 3552 4052 4408 5543 6224 6415
Size: 219 1634 873 500 356 1135 681 191
Cuts at: 6415 826
Size: 1471
Fragments arranged by size:
1634 1471 1135 873 681 500 356 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 4387 1872
Size: 2515 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 3900 5164 6065 6327 820
Size: 197 926 1242 715 1264 901 262 1553
Fragments arranged by size:
1553 1264 1242 926 901 715 262 197
SnaBI TAC'GTA
Cuts at: 493 4851 493
Size: 4358 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7060
SphI G_CATG'C
Cuts at: 1132 4418 1132
Size: 3286 3774
SspI AAT'ATT
Cuts at: 5 52 2643 4984 5
Size: 47 2591 2341 2081
Fragments arranged by size:
2591 2341 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7060
StyI C'CwwG_G
Cuts at: 513 1065 4499 513
Size: 552 3434 3074
Fragments arranged by size:
3434 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3804 5087 5104 5257 5442 5887 325
Size: 1255 2224 1283 17 153 185 445 1498
Fragments arranged by size:
2224 1498 1283 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2838 4285
Size: 274 80 33 51 1443 153 708 1447
Cuts at: 4285 5306 7018 96
Size: 1021 1712 138
Fragments arranged by size:
1712 1447 1443 1021 708 274 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 3268 3300 7020 7052 2602
Size: 32 634 32 3720 32 2610
Fragments arranged by size:
3720 2610 634 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7060
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 4385 1870
Size: 2515 4545
XmnI GAAnn'nnTTC
Cuts at: 3194 4143 5189 3194
Size: 949 1046 5065
Fragments arranged by size:
5065 1046 949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4868 276
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI