Restriction map for
pFN21AB5712
(Circular) (Six-base) MAPSORT of: pFN21AB5712.seq Check: 8605 from: 1 to: 6865
pFN21AB5712 6865 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2596 2740 3670 2596
Size: 144 930 5791
Fragments arranged by size:
5791 930 144
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2970 4675 278
Size: 53 83 186 936 1434 1705 2468
Fragments arranged by size:
2468 1705 1434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 1937
Size: 2271 4594
Acc65I G'GTAC_C
Cuts at: 2397 3318 4186 2397
Size: 921 868 5076
Fragments arranged by size:
5076 921 868
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 2982 4056 4125 4928 6312 6580 1632
Size: 1235 115 1074 69 803 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1074 803 268 115 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6668
AflIII A'CryG_T
Cuts at: 1203 5764 6741 1203
Size: 4561 977 1327
Fragments arranged by size:
4561 1327 977
AgeI A'CCGG_T
Cuts at: 2011 5696 2011
Size: 3685 3180
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 3500 1184
Size: 948 1368 4549
Fragments arranged by size:
4549 1368 948
AlwNI CAG_nnn'CTG
Cuts at: 2212 2495 2896 3029 3796 3913 4608 6180 2212
Size: 283 401 133 767 117 695 1572 2897
Fragments arranged by size:
2897 1572 767 695 401 283 133 117
ApaI G_GGCC'C
Cuts at: 2956 3269 3535 2956
Size: 313 266 6286
Fragments arranged by size:
6286 313 266
ApaLI G'TGCA_C
Cuts at: 3863 4922 6078 3863
Size: 1059 1156 4650
Fragments arranged by size:
4650 1156 1059
ApoI r'AATT_y
Cuts at: 1493 2637 4174 4400 4552 6661 1493
Size: 1144 1537 226 152 2109 1697
Fragments arranged by size:
2109 1697 1537 1144 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5419 160
Size: 872 4387 1606
Fragments arranged by size:
4387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2600 3543 4190 1870
Size: 73 198 459 943 647 4545
Fragments arranged by size:
4545 943 647 459 198 73
AvrII C'CTAG_G
Cuts at: 3778 3778
Size: 6865
BamHI G'GATC_C
Cuts at: 4195 4562 4195
Size: 367 6498
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 3247 3276 3318 4186
Size: 325 217 627 610 850 29 42 868
Cuts at: 4186 5641 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 868 850 627 610 325 217
42 29
BanII G_rGCy'C
Cuts at: 1391 2181 2219 2921 2956 3051 3114 3269 3535
Size: 790 38 702 35 95 63 155 266
Cuts at: 3535 3821 4184 1391
Size: 286 363 4072
Fragments arranged by size:
4072 790 702 363 286 266 155 95
63 38 35
BbeI G_GCGC'C
Cuts at: 3280 3280
Size: 6865
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 2979 3558 928
Size: 971 1071 9 579 4235
Fragments arranged by size:
4235 1071 971 579 9
BbvCI CC'TCA_GC
Cuts at: 3345 3755 3846 3345
Size: 410 91 6364
Fragments arranged by size:
6364 410 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5056 5090 1903
Size: 34 3119 34 3678
Fragments arranged by size:
3678 3119 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 3391 1548
Size: 1843 5022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5476 136
Size: 107 122 71 631 605 3804 1525
Fragments arranged by size:
3804 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3469 6860 3469
Size: 3391 3474
BlpI GC'TnA_GC
Cuts at: 2390 2462 3176 4282 2390
Size: 72 714 1106 4973
Fragments arranged by size:
4973 1106 714 72
Bme1580I G_kGCm'C
Cuts at: 1790 2510 2956 3269 3535 3867 4926 6082 1790
Size: 720 446 313 266 332 1059 1156 2573
Fragments arranged by size:
2573 1156 1059 720 446 332 313 266
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 4596 5554
Size: 416 53 83 399 24 1574 1605 958
Cuts at: 5554 442
Size: 1753
Fragments arranged by size:
1753 1605 1574 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4015 4015
Size: 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2189 2203 2742 3090 3507 5525 1239
Size: 866 84 14 539 348 417 2018 2579
Fragments arranged by size:
2579 2018 866 539 417 348 84 14
Bpu10I CC'TnA_GC
Cuts at: 2213 3045 3345 3755 3846 3945 2213
Size: 832 300 410 91 99 5133
Fragments arranged by size:
5133 832 410 300 99 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3281 3612 3878 4990 5855 6153 3281
Size: 331 266 1112 865 298 3993
Fragments arranged by size:
3993 1112 865 331 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 2166 2861 3560 3834 5528 882
Size: 617 513 154 695 699 274 1694 2219
Fragments arranged by size:
2219 1694 699 695 617 513 274 154
BsaAI yAC'GTr
Cuts at: 493 1204 2533 4656 493
Size: 711 1329 2123 2702
Fragments arranged by size:
2702 2123 1329 711
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 2882 2967 3277
Size: 53 83 186 936 840 509 85 310
Cuts at: 3277 4672 5054 5689 275
Size: 1395 382 635 1451
Fragments arranged by size:
1451 1395 936 840 635 509 382 310
186 85 83 53
BsaWI w'CCGG_w
Cuts at: 2011 2863 5298 5696 5970 6117 6528 2011
Size: 852 2435 398 274 147 411 2348
Fragments arranged by size:
2435 2348 852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2715 2745 3017
Size: 30 165 30 987 30 295 30 272
Cuts at: 3017 3047 1178
Size: 30 4996
Fragments arranged by size:
4996 987 295 272 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2471 2780 2929 1186
Size: 1183 102 309 149 5122
Fragments arranged by size:
5122 1183 309 149 102
BseYI C'CCAG_C
Cuts at: 2537 3008 3073 3085 3205 3596 3617 3632 3934
Size: 471 65 12 120 391 21 15 302
Cuts at: 3934 6068 6564 2537
Size: 2134 496 2838
Fragments arranged by size:
2838 2134 496 471 391 302 120 65
21 15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2364 2630 1163
Size: 573 628 266 5398
Fragments arranged by size:
5398 628 573 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3215 4315 5076 6104 1540
Size: 275 186 1214 1100 761 1028 2301
Fragments arranged by size:
2301 1214 1100 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2219 2601 3297 3821 3867 3890 4113 4926
Size: 38 382 696 524 46 23 223 813
Cuts at: 4926 5011 6082 2181
Size: 85 1071 2964
Fragments arranged by size:
2964 1071 813 696 524 382 223 85
46 38 23
BsmI GAATG_Cn'
Cuts at: 4376 4469 4376
Size: 93 6772
BsmBI CGTCTCn'nnnn_
Cuts at: 2718 2718
Size: 6865
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2219 2510 2601 2921 2956 3051
Size: 399 391 38 291 91 320 35 95
Cuts at: 3051 3114 3269 3297 3535 3821 3867 3890 4113
Size: 63 155 28 238 286 46 23 223
Cuts at: 4113 4184 4926 5011 6082 1391
Size: 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 320 291 286
238 223 155 95 91 85 71 63
46 38 35 28 23
BspEI T'CCGG_A
Cuts at: 6528 6528
Size: 6865
BspHI T'CATG_A
Cuts at: 4754 6484 4754
Size: 1730 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2247 2385 2596 2740 3509 3670 3907
Size: 1280 123 138 211 144 769 161 237
Cuts at: 3907 4220 844
Size: 313 3489
Fragments arranged by size:
3489 1280 769 313 237 211 161 144
138 123
BsrBI CCG'CTC
Cuts at: 1394 1823 4752 1394
Size: 429 2929 3507
Fragments arranged by size:
3507 2929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5360 5534 65
Size: 1183 4112 174 1396
Fragments arranged by size:
4112 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 3183 3210 5509 5696 1700
Size: 110 201 1172 27 2299 187 2869
Fragments arranged by size:
2869 2299 1172 201 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 3432 96
Size: 3336 3529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 4925 5937 4925
Size: 1012 5853
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3871 3913 794
Size: 3077 42 3746
Fragments arranged by size:
3746 3077 42
BstBI TT'CG_AA
Cuts at: 5669 5669
Size: 6865
BstEII G'GTnAC_C
Cuts at: 1184 3938 1184
Size: 2754 4111
BstXI CCAn_nnnn'nTGG
Cuts at: 2537 2537
Size: 6865
BstYI r'GATC_y
Cuts at: 1972 2439 2520 2541 2712 2853 3469 4195 4562
Size: 467 81 21 171 141 616 726 367
Cuts at: 4562 4947 4964 6416 6502 6514 6860 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 726 616 467 385 367 346
171 141 86 81 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2516 2889 2516
Size: 373 6492
BtgI C'CryG_G
Cuts at: 513 1065 2004 3646 3689 513
Size: 552 939 1642 43 3689
Fragments arranged by size:
3689 1642 939 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6126
Fragments arranged by size:
6126 683 56
BtsI GCAGTG_nn'
Cuts at: 3727 4381 5175 5195 3727
Size: 654 794 20 5397
Fragments arranged by size:
5397 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6830
DraI TTT'AAA
Cuts at: 4169 4169
Size: 6865
DraIII CAC_nnn'GTG
Cuts at: 2668 3871 2668
Size: 1203 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2732 5872 809
Size: 507 1416 3140 1802
Fragments arranged by size:
3140 1802 1416 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3212 4312 5201
Size: 54 1425 50 275 18 1382 1100 889
Cuts at: 5201 6830 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1382 1100 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3212 4312 1537
Size: 275 1400 1100 4090
Fragments arranged by size:
4090 1400 1100 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2408 3720 4101 4795 1353
Size: 1034 21 1312 381 694 3423
Fragments arranged by size:
3423 1312 1034 694 381 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3829 3956 5452 5826 5972 387
Size: 1064 138 2240 127 1496 374 146 1280
Fragments arranged by size:
2240 1496 1280 1064 374 146 138 127
EcoICRI GAG'CTC
Cuts at: 2179 2217 3819 2179
Size: 38 1602 5225
Fragments arranged by size:
5225 1602 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2189 2203 2742 2867 2982 3090
Size: 393 473 84 14 539 125 115 108
Cuts at: 3090 3507 4056 4125 4928 5525 6312 6580 1239
Size: 417 549 69 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 597 549 539 473 417
393 268 125 115 108 84 69 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 3800 2252
Size: 1548 5317
EcoO109I rG'GnC_Cy
Cuts at: 2209 2893 2952 2953 3349 3532 3580 3600 3919
Size: 684 59 1 396 183 48 20 319
Cuts at: 3919 4006 4014 2209
Size: 87 8 5060
Fragments arranged by size:
5060 684 396 319 183 87 59 48
20 8 1
EcoRI G'AATT_C
Cuts at: 4174 4552 4174
Size: 378 6487
FseI GG_CCGG'CC
Cuts at: 3214 3214
Size: 6865
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 3280 3881 6012 1678
Size: 1602 601 2131 2531
Fragments arranged by size:
2531 2131 1602 601
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2697 2729 2872 2904 3017
Size: 32 135 32 1125 32 143 32 113
Cuts at: 3017 3049 3294 3326 3584 3616 5506 5538 5580
Size: 32 245 32 258 32 1890 32 42
Cuts at: 5580 5612 1373
Size: 32 2626
Fragments arranged by size:
2626 1890 1125 258 245 143 135 113
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6865
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KasI G'GCGC_C
Cuts at: 3276 3276
Size: 6865
KpnI G_GTAC'C
Cuts at: 2401 3322 4190 2401
Size: 921 868 5076
Fragments arranged by size:
5076 921 868
MfeI C'AATT_G
Cuts at: 2653 4464 2653
Size: 1811 5054
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 3125 3390 4174 5979 6163 1305
Size: 1128 692 265 784 1805 184 2007
Fragments arranged by size:
2007 1805 1128 784 692 265 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5043
Fragments arranged by size:
5043 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2415 2481 3482 4823 5182 5341 518
Size: 648 1249 66 1001 1341 359 159 2042
Fragments arranged by size:
2042 1341 1249 1001 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2101 2348 2492 2614 3285 4281 4958 6106
Size: 495 247 144 122 671 996 677 1148
Cuts at: 6106 6351 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 996 677 671 495 247 245
144 122
NaeI GCC'GGC
Cuts at: 3185 3212 3185
Size: 27 6838
NarI GG'CG_CC
Cuts at: 3277 3277
Size: 6865
NcoI C'CATG_G
Cuts at: 513 1065 2004 3646 513
Size: 552 939 1642 3732
Fragments arranged by size:
3732 1642 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6865
NgoMIV G'CCGG_C
Cuts at: 3183 3210 3183
Size: 27 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NspI r_CATG'y
Cuts at: 1132 1229 2433 3481 4223 5768 1132
Size: 97 1204 1048 742 1545 2229
Fragments arranged by size:
2229 1545 1204 1048 742 97
PasI CC'CwG_GG
Cuts at: 1348 2949 1348
Size: 1601 5264
PciI A'CATG_T
Cuts at: 5764 5764
Size: 6865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6865
PfoI T'CCnGG_A
Cuts at: 2895 3001 2895
Size: 106 6759
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PmlI CAC'GTG
Cuts at: 2533 2533
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2893 3580 3600 3919 2893
Size: 687 20 319 5839
Fragments arranged by size:
5839 687 319 20
PshAI GACnn'nnGTC
Cuts at: 1613 3132 1613
Size: 1519 5346
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspOMI G'GGCC_C
Cuts at: 2952 3265 3531 2952
Size: 313 266 6286
Fragments arranged by size:
6286 313 266
PspXI vC'TCGA_Gb
Cuts at: 2141 2600 2141
Size: 459 6406
PstI C_TGCA'G
Cuts at: 830 2076 2528 2712 3667 3909 3918 4217 830
Size: 1246 452 184 955 242 9 299 3478
Fragments arranged by size:
3478 1246 955 452 299 242 184 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2101 2348 2614 2101
Size: 247 266 6352
Fragments arranged by size:
6352 266 247
SacI G_AGCT'C
Cuts at: 2181 2219 3821 2181
Size: 38 1602 5225
Fragments arranged by size:
5225 1602 38
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SanDI GG'GwC_CC
Cuts at: 3600 3600
Size: 6865
SapI GCTCTTCn'nnn_
Cuts at: 4101 4101
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 2076 4217 2076
Size: 2141 4724
ScaI AGT'ACT
Cuts at: 5113 6825 5113
Size: 1712 5153
SfcI C'TryA_G
Cuts at: 826 1045 2072 2225 2524 2708 3370 3559 3663
Size: 219 1027 153 299 184 662 189 104
Cuts at: 3663 3881 3905 3914 4213 5348 6029 6220 826
Size: 218 24 9 299 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 662 299 299 219
218 191 189 184 153 104 24 9
SfoI GGC'GCC
Cuts at: 3278 3278
Size: 6865
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 3183 1700
Size: 1483 5382
SmaI CCC'GGG
Cuts at: 1872 4192 1872
Size: 2320 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 2600 3296 3627 3893 4969
Size: 197 926 198 459 696 331 266 1076
Cuts at: 4969 5870 6132 820
Size: 901 262 1553
Fragments arranged by size:
1553 1076 926 901 696 459 331 266
262 198 197
SnaBI TAC'GTA
Cuts at: 493 4656 493
Size: 4163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 2433 4223 1132
Size: 1301 1790 3774
Fragments arranged by size:
3774 1790 1301
SspI AAT'ATT
Cuts at: 5 52 4789 5
Size: 47 4737 2081
Fragments arranged by size:
4737 2081 47
StuI AGG'CCT
Cuts at: 1517 3162 1517
Size: 1645 5220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 2571 3037 3646 3778 4304
Size: 552 939 292 275 466 609 132 526
Cuts at: 4304 513
Size: 3074
Fragments arranged by size:
3074 939 609 552 526 466 292 275
132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2699 3437 3472 3584 3926 4892 4909
Size: 1255 1119 738 35 112 342 966 17
Cuts at: 4909 5062 5247 5692 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 966 738 445 342 185
153 112 35 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3432 3996 5111
Size: 274 80 33 51 1443 1455 564 1115
Cuts at: 5111 6823 96
Size: 1712 138
Fragments arranged by size:
1712 1455 1443 1115 564 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2654 2674 2686 2706 6825 6857 2654
Size: 20 12 20 4119 32 2662
Fragments arranged by size:
4119 2662 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 4201 4201
Size: 6865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 3373 2578
Size: 795 6070
XhoI C'TCGA_G
Cuts at: 1943 2141 2600 1943
Size: 198 459 6208
Fragments arranged by size:
6208 459 198
XmaI C'CCGG_G
Cuts at: 1870 4190 1870
Size: 2320 4545
XmnI GAAnn'nnTTC
Cuts at: 4994 4994
Size: 6865
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2968 4673 276
Size: 53 83 186 936 1434 1705 2468
Fragments arranged by size:
2468 1705 1434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PsrI RsrII SacII
SexAI SfiI SrfI SwaI