Restriction map for
pFN21AB5710
(Circular) (Six-base) MAPSORT of: pFN21AB5710.seq Check: 6547 from: 1 to: 6856
pFN21AB5710 6856 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2983 3097 2983
Size: 114 6742
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4199 1937
Size: 2262 4594
Acc65I G'GTAC_C
Cuts at: 2690 4177 2690
Size: 1487 5369
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2240 2333 2700 2746 2903 3380 3618
Size: 593 15 93 367 46 157 477 238
Cuts at: 3618 4919 6303 6571 1632
Size: 1301 1384 268 1917
Fragments arranged by size:
1917 1384 1301 593 477 367 268 238
157 93 46 15
AfeI AGC'GCT
Cuts at: 2582 2582
Size: 6856
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 5755 6732 1203
Size: 4552 977 1327
Fragments arranged by size:
4552 1327 977
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 2488 2521 5585 2488
Size: 33 3064 3759
Fragments arranged by size:
3759 3064 33
AleI CACnn'nnGTG
Cuts at: 1166 3335 1166
Size: 2169 4687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2664 3845 4034 1184
Size: 1480 1181 189 4006
Fragments arranged by size:
4006 1480 1181 189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3900 3932 3900
Size: 32 6824
AlwNI CAG_nnn'CTG
Cuts at: 2095 2635 2851 2881 3118 3996 4084 4599 6171
Size: 540 216 30 237 878 88 515 1572
Cuts at: 6171 2095
Size: 2780
Fragments arranged by size:
2780 1572 878 540 515 237 216 88
30
ApaI G_GGCC'C
Cuts at: 3550 3550
Size: 6856
ApaLI G'TGCA_C
Cuts at: 4913 6069 4913
Size: 1156 5700
ApoI r'AATT_y
Cuts at: 1493 4165 4391 4543 6652 1493
Size: 2672 226 152 2109 1697
Fragments arranged by size:
2672 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 4181 1870
Size: 73 2238 4545
Fragments arranged by size:
4545 2238 73
BamHI G'GATC_C
Cuts at: 3319 4186 4553 3319
Size: 867 367 5622
Fragments arranged by size:
5622 867 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 2958 3868 4045 4177
Size: 325 217 627 903 268 910 177 132
Cuts at: 4177 5632 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 910 903 627 325 268 217
177 132
BanII G_rGCy'C
Cuts at: 1391 2337 2463 3074 3375 3550 4175 1391
Size: 946 126 611 301 175 625 4072
Fragments arranged by size:
4072 946 625 611 301 175 126
BbeI G_GCGC'C
Cuts at: 2962 4049 2962
Size: 1087 5769
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2904 928
Size: 971 1005 4880
Fragments arranged by size:
4880 1005 971
BbvCI CC'TCA_GC
Cuts at: 2556 2975 2556
Size: 419 6437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 1903
Size: 34 3110 34 3678
Fragments arranged by size:
3678 3110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4748 5958 4748
Size: 1210 5646
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2870 2967 3069
Size: 107 122 71 631 605 1198 97 102
Cuts at: 3069 3149 5467 136
Size: 80 2318 1525
Fragments arranged by size:
2318 1525 1198 631 605 122 107 102
97 80 71
BglII A'GATC_T
Cuts at: 3658 6851 3658
Size: 3193 3663
BlpI GC'TnA_GC
Cuts at: 2022 4273 2022
Size: 2251 4605
Bme1580I G_kGCm'C
Cuts at: 1790 3550 3871 4917 6073 1790
Size: 1760 321 1046 1156 2573
Fragments arranged by size:
2573 1760 1156 1046 321
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6856
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4054 4587 5545
Size: 416 53 83 399 24 2637 533 958
Cuts at: 5545 442
Size: 1753
Fragments arranged by size:
2637 1753 958 533 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6856
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 6856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2615 2759 2789 2831 2954 3026 3068
Size: 911 465 144 30 42 123 72 42
Cuts at: 3068 3110 3203 3422 3491 3647 3683 3719 3858
Size: 42 93 219 69 156 36 36 139
Cuts at: 3858 3953 5516 1239
Size: 95 1563 2579
Fragments arranged by size:
2579 1563 911 465 219 156 144 139
123 95 93 72 69 42 42 42
36 36 30
Bpu10I CC'TnA_GC
Cuts at: 2096 2556 2975 3374 2096
Size: 460 419 399 5578
Fragments arranged by size:
5578 460 419 399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4981 5846 6144 4981
Size: 865 298 5693
Fragments arranged by size:
5693 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 2705 5519 882
Size: 617 541 665 2814 2219
Fragments arranged by size:
2814 2219 665 617 541
BsaAI yAC'GTr
Cuts at: 493 1204 3808 4647 493
Size: 711 2604 839 2702
Fragments arranged by size:
2702 2604 839 711
BsaBI GATnn'nnATC
Cuts at: 6850 6850
Size: 6856
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2959 4046 4117 4663
Size: 53 83 186 936 1426 1087 71 546
Cuts at: 4663 5045 5680 275
Size: 382 635 1451
Fragments arranged by size:
1451 1426 1087 936 635 546 382 186
83 71 53
BsaWI w'CCGG_w
Cuts at: 2838 5289 5687 5961 6108 6519 2838
Size: 2451 398 274 147 411 3175
Fragments arranged by size:
3175 2451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3176 3206 3209 3239 1178
Size: 30 165 30 1773 30 3 30 4795
Fragments arranged by size:
4795 1773 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 2573 2636 2768 3152 3275 3467 3707
Size: 1378 9 63 132 384 123 192 240
Cuts at: 3707 3752 3803 4052 1186
Size: 45 51 249 3990
Fragments arranged by size:
3990 1378 384 249 240 192 132 123
63 51 45 9
BseYI C'CCAG_C
Cuts at: 2359 2733 2924 3084 3276 4031 6059 6555 2359
Size: 374 191 160 192 755 2028 496 2660
Fragments arranged by size:
2660 2028 755 496 374 192 191 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3131 3439 1163
Size: 573 1395 308 4580
Fragments arranged by size:
4580 1395 573 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3435 3439 4306 5067 6095 1540
Size: 275 186 1434 4 867 761 1028 2301
Fragments arranged by size:
2301 1434 1028 867 761 275 186 4
BsiHKAI G_wGCw'C
Cuts at: 2337 2463 3375 4917 5002 6073 2337
Size: 126 912 1542 85 1071 3120
Fragments arranged by size:
3120 1542 1071 912 126 85
BsmI GAATG_Cn'
Cuts at: 2653 3176 4367 4460 2653
Size: 523 1191 93 5049
Fragments arranged by size:
5049 1191 523 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2076 2492 3374 3497 2076
Size: 416 882 123 5435
Fragments arranged by size:
5435 882 416 123
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2463 3074 3375 3550 3871 4175
Size: 399 547 126 611 301 175 321 304
Cuts at: 4175 4917 5002 6073 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 547 399 321 304
301 175 126 85
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 2409 2463 3649 4745 6475 2409
Size: 54 1186 1096 1730 2790
Fragments arranged by size:
2790 1730 1186 1096 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2520 2983 3097 3570 4211 844
Size: 1676 463 114 473 641 3489
Fragments arranged by size:
3489 1676 641 473 463 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 2665 3068 3220 3235 3445 4042
Size: 429 776 66 403 152 15 210 597
Cuts at: 4042 4743 1394
Size: 701 3507
Fragments arranged by size:
3507 776 701 597 429 403 210 152
66 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 5351 5525 65
Size: 1183 4103 174 1396
Fragments arranged by size:
4103 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2673 2955 3020 3513 5500 5687 1700
Size: 110 863 282 65 493 1987 187 2869
Fragments arranged by size:
2869 1987 863 493 282 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6856
BssHII G'CGCG_C
Cuts at: 1925 3079 3486 3488 1925
Size: 1154 407 2 5293
Fragments arranged by size:
5293 1154 407 2
BssSI C'ACGA_G
Cuts at: 4916 5928 4916
Size: 1012 5844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2608 3118 3754 794
Size: 1814 510 636 3896
Fragments arranged by size:
3896 1814 636 510
BstBI TT'CG_AA
Cuts at: 5660 5660
Size: 6856
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3337 2011
Size: 1326 5530
BstYI r'GATC_y
Cuts at: 1972 2182 2590 3319 3357 3658 4186 4553 4938
Size: 210 408 729 38 301 528 367 385
Cuts at: 4938 4955 6407 6493 6505 6851 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 729 528 408 385 367 346
301 210 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2088 3890 2088
Size: 1802 5054
BtgI C'CryG_G
Cuts at: 513 1065 3287 3336 3493 3783 3837 4102 513
Size: 552 2222 49 157 290 54 265 3267
Fragments arranged by size:
3267 2222 552 290 265 157 54 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6117
Fragments arranged by size:
6117 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 4372 5166 5186 2703
Size: 1669 794 20 4373
Fragments arranged by size:
4373 1669 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6821
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 809
Size: 507 4547 1802
Fragments arranged by size:
4547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3432 3854
Size: 54 1425 50 275 18 1192 410 422
Cuts at: 3854 4303 5192 6821 8
Size: 449 889 1629 43
Fragments arranged by size:
1629 1425 1192 889 449 422 410 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3432 4303 1537
Size: 275 1620 871 4090
Fragments arranged by size:
4090 1620 871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 4786 1353
Size: 924 2509 3423
Fragments arranged by size:
3423 2509 924
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3245 3745 5443 5817 5963 387
Size: 1064 138 1656 500 1698 374 146 1280
Fragments arranged by size:
1698 1656 1280 1064 500 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2461 3373 2335
Size: 126 912 5818
Fragments arranged by size:
5818 912 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2225 2240 2333 2615 2700 2746
Size: 393 518 75 15 93 282 85 46
Cuts at: 2746 2759 2789 2831 2903 2954 3026 3068 3110
Size: 13 30 42 72 51 72 42 42
Cuts at: 3110 3203 3380 3422 3491 3618 3647 3683 3719
Size: 93 177 42 69 127 29 36 36
Cuts at: 3719 3858 3953 4919 5516 6303 6571 1239
Size: 139 95 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 597 518 393 282 268
177 139 127 95 93 93 85 75
72 72 69 51 46 42 42 42
42 36 36 30 29 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2546 2618 2864 2546
Size: 72 246 6538
Fragments arranged by size:
6538 246 72
EcoO109I rG'GnC_Cy
Cuts at: 2092 2189 2878 3559 2092
Size: 97 689 681 5389
Fragments arranged by size:
5389 689 681 97
EcoRI G'AATT_C
Cuts at: 4165 4543 4165
Size: 378 6478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 6856
FseI GG_CCGG'CC
Cuts at: 2677 2677
Size: 6856
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 2584 2794 2852 2962 3466 4049 6003 1678
Size: 906 210 58 110 504 583 1954 2531
Fragments arranged by size:
2531 1954 906 583 504 210 110 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2507 2539 3311
Size: 32 135 32 902 32 1 32 772
Cuts at: 3311 3343 5497 5529 5571 5603 1373
Size: 32 2154 32 42 32 2626
Fragments arranged by size:
2626 2154 902 772 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 4200 4446 669
Size: 988 281 1359 903 246 3079
Fragments arranged by size:
3079 1359 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 3644 748
Size: 2896 3960
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KasI G'GCGC_C
Cuts at: 2958 4045 2958
Size: 1087 5769
KpnI G_GTAC'C
Cuts at: 2694 4181 2694
Size: 1487 5369
MfeI C'AATT_G
Cuts at: 2744 4455 2744
Size: 1711 5145
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3480 3949 5970 6154 1305
Size: 2175 469 2021 184 2007
Fragments arranged by size:
2175 2021 2007 469 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5034
Fragments arranged by size:
5034 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3335 3827 4814 5173 5332 518
Size: 648 843 1326 492 987 359 159 2042
Fragments arranged by size:
2042 1326 987 843 648 492 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2353 2605 2737 2932 3004 3088 3100
Size: 497 250 252 132 195 72 84 12
Cuts at: 3100 3130 3280 3289 3400 3853 3860 3964 4081
Size: 30 150 9 111 453 7 104 117
Cuts at: 4081 4272 4949 6097 6342 1606
Size: 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 497 453 252 250 245
195 191 150 132 117 111 104 84
72 30 12 9 7
NaeI GCC'GGC
Cuts at: 2675 2957 3022 2675
Size: 282 65 6509
Fragments arranged by size:
6509 282 65
NarI GG'CG_CC
Cuts at: 2959 4046 2959
Size: 1087 5769
NcoI C'CATG_G
Cuts at: 513 1065 3783 3837 513
Size: 552 2718 54 3532
Fragments arranged by size:
3532 2718 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NgoMIV G'CCGG_C
Cuts at: 2673 2955 3020 2673
Size: 282 65 6509
Fragments arranged by size:
6509 282 65
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6856
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 2438 4214 5759 1132
Size: 97 1209 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 1209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6856
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3499 1108
Size: 2391 4465
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2189 3559 2092
Size: 97 1370 5389
Fragments arranged by size:
5389 1370 97
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PspOMI G'GGCC_C
Cuts at: 3546 3546
Size: 6856
PstI C_TGCA'G
Cuts at: 830 2835 3123 3285 3462 3567 3579 3747 4208
Size: 2005 288 162 177 105 12 168 461
Cuts at: 4208 830
Size: 3478
Fragments arranged by size:
3478 2005 461 288 177 168 162 105
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 2353 2605 2737 2932 3004 3088 3280 3400 3860
Size: 252 132 195 72 84 192 120 460
Cuts at: 3860 2353
Size: 5349
Fragments arranged by size:
5349 460 252 195 192 132 120 84
72
SacI G_AGCT'C
Cuts at: 2337 2463 3375 2337
Size: 126 912 5818
Fragments arranged by size:
5818 912 126
SacII CC_GC'GG
Cuts at: 3290 3290
Size: 6856
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SapI GCTCTTCn'nnn_
Cuts at: 2277 2277
Size: 6856
SbfI CC_TGCA'GG
Cuts at: 3579 4208 3579
Size: 629 6227
ScaI AGT'ACT
Cuts at: 2249 5104 6816 2249
Size: 2855 1712 2289
Fragments arranged by size:
2855 2289 1712
SexAI A'CCwGG_T
Cuts at: 2045 2045
Size: 6856
SfcI C'TryA_G
Cuts at: 826 1045 2831 3119 3281 3458 3563 3575 3743
Size: 219 1786 288 162 177 105 12 168
Cuts at: 3743 4204 5339 6020 6211 826
Size: 461 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 681 461 288 219 191
177 168 162 105 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 3149 3149
Size: 6856
SfoI GGC'GCC
Cuts at: 2960 4047 2960
Size: 1087 5769
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 4183 1872
Size: 2311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4960 5861 6123 820
Size: 197 926 3017 901 262 1553
Fragments arranged by size:
3017 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4647 493
Size: 4154 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 4780 5
Size: 47 4728 2081
Fragments arranged by size:
4728 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6856
StyI C'CwwG_G
Cuts at: 513 1065 3783 3837 4110 4295 513
Size: 552 2718 54 273 185 3074
Fragments arranged by size:
3074 2718 552 273 185 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3721 4883 4900 5053 5238 5683 325
Size: 1255 2141 1162 17 153 185 445 1498
Fragments arranged by size:
2141 1498 1255 1162 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 3684 5102
Size: 274 80 33 51 1443 270 1437 1418
Cuts at: 5102 6814 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1437 1418 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6816 6848 6816
Size: 32 6824
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6856
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2193 2193
Size: 6856
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6856
XmaI C'CCGG_G
Cuts at: 1870 4181 1870
Size: 2311 4545
XmnI GAAnn'nnTTC
Cuts at: 2427 4985 2427
Size: 2558 4298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SanDI SrfI SwaI