Restriction map for
pFN21AB5709
(Circular) (Six-base) MAPSORT of: pFN21AB5709.seq Check: 5868 from: 1 to: 7057
pFN21AB5709 7057 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4867 278
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4400 1937
Size: 2463 4594
Acc65I G'GTAC_C
Cuts at: 4378 4378
Size: 7057
AclI AA'CG_TT
Cuts at: 1563 5184 5557 1563
Size: 3621 373 3063
Fragments arranged by size:
3621 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 3640 3948 4057 4340 5120 6504 6772
Size: 452 1556 308 109 283 780 1384 268
Cuts at: 6772 1632
Size: 1917
Fragments arranged by size:
1917 1556 1384 780 452 308 283 268
109
AfeI AGC'GCT
Cuts at: 3833 3833
Size: 7057
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6860
AflIII A'CryG_T
Cuts at: 1203 2271 5956 6933 1203
Size: 1068 3685 977 1327
Fragments arranged by size:
3685 1327 1068 977
AgeI A'CCGG_T
Cuts at: 5888 5888
Size: 7057
AhdI GACnn_n'nnGTC
Cuts at: 5786 5786
Size: 7057
AleI CACnn'nnGTG
Cuts at: 1166 3797 1166
Size: 2631 4426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7057
AlwNI CAG_nnn'CTG
Cuts at: 4193 4800 6372 4193
Size: 607 1572 4878
Fragments arranged by size:
4878 1572 607
ApaI G_GGCC'C
Cuts at: 3305 3813 3873 3305
Size: 508 60 6489
Fragments arranged by size:
6489 508 60
ApaLI G'TGCA_C
Cuts at: 5114 6270 5114
Size: 1156 5901
ApoI r'AATT_y
Cuts at: 1493 4366 4592 4744 6853 1493
Size: 2873 226 152 2109 1697
Fragments arranged by size:
2873 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5611 160
Size: 872 4579 1606
Fragments arranged by size:
4579 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
AvaI C'yCGr_G
Cuts at: 1870 1943 3264 4382 1870
Size: 73 1321 1118 4545
Fragments arranged by size:
4545 1321 1118 73
BamHI G'GATC_C
Cuts at: 2748 4387 4754 2748
Size: 1639 367 5051
Fragments arranged by size:
5051 1639 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2458 4378 5833 618
Size: 325 217 627 671 1920 1455 1842
Fragments arranged by size:
1920 1842 1455 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2583 3305 3687 3813 3873 4203 4218 4376
Size: 1192 722 382 126 60 330 15 158
Cuts at: 4376 1391
Size: 4072
Fragments arranged by size:
4072 1192 722 382 330 158 126 60
15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 3903 4089 928
Size: 971 231 1773 186 3896
Fragments arranged by size:
3896 1773 971 231 186
BbvCI CC'TCA_GC
Cuts at: 3324 3540 4194 3324
Size: 216 654 6187
Fragments arranged by size:
6187 654 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5248 5282 1903
Size: 34 3311 34 3678
Fragments arranged by size:
3678 3311 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4949 6159 4949
Size: 1210 5847
BclI T'GATC_A
Cuts at: 1548 3075 1548
Size: 1527 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5668 136
Size: 107 122 71 631 605 3996 1525
Fragments arranged by size:
3996 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3009 7052 3009
Size: 4043 3014
BlpI GC'TnA_GC
Cuts at: 4474 4474
Size: 7057
Bme1580I G_kGCm'C
Cuts at: 1790 3305 3813 3873 4352 5118 6274 1790
Size: 1515 508 60 479 766 1156 2573
Fragments arranged by size:
2573 1515 1156 766 508 479 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3734 4788 5746
Size: 416 53 83 399 24 2317 1054 958
Cuts at: 5746 442
Size: 1753
Fragments arranged by size:
2317 1753 1054 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3889 3889
Size: 7057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2868 3239 3368 3701 3889 5717 1239
Size: 1629 371 129 333 188 1828 2579
Fragments arranged by size:
2579 1828 1629 371 333 188 129
Bpu10I CC'TnA_GC
Cuts at: 2633 3324 3540 4194 2633
Size: 691 216 654 5496
Fragments arranged by size:
5496 691 654 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 3257 3334 4093 5182 6047 6345 2768
Size: 489 77 759 1089 865 298 3480
Fragments arranged by size:
3480 1089 865 759 489 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5720 882
Size: 617 4221 2219
Fragments arranged by size:
4221 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4848 493
Size: 711 3644 2702
Fragments arranged by size:
3644 2702 711
BsaBI GATnn'nnATC
Cuts at: 7051 7051
Size: 7057
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4864 5246 5881 275
Size: 53 83 186 936 3331 382 635 1451
Fragments arranged by size:
3331 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2520 2745 5490 5888 6162 6309 6720 2520
Size: 225 2745 398 274 147 411 2857
Fragments arranged by size:
2857 2745 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3919 3949 1178
Size: 30 165 30 2516 30 4286
Fragments arranged by size:
4286 2516 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3371 3557 3560 4025 4028 4208 4211 1186
Size: 2185 186 3 465 3 180 3 4032
Fragments arranged by size:
4032 2185 465 186 180 3 3 3
BseYI C'CCAG_C
Cuts at: 2651 2918 3225 3686 3814 4057 4095 6260 6756
Size: 267 307 461 128 243 38 2165 496
Cuts at: 6756 2651
Size: 2952
Fragments arranged by size:
2952 2165 496 461 307 267 243 128
38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 3122 3614 1163
Size: 573 1254 132 492 4606
Fragments arranged by size:
4606 1254 573 492 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4276 4507 5268 6296 1540
Size: 275 186 2275 231 761 1028 2301
Fragments arranged by size:
2301 2275 1028 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 2583 2639 2817 4218 5118 5203 6274 2583
Size: 56 178 1401 900 85 1071 3366
Fragments arranged by size:
3366 1401 1071 900 178 85 56
BsmI GAATG_Cn'
Cuts at: 4568 4661 4568
Size: 93 6964
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 2639 2817 3305 3687 3813 3873
Size: 399 793 56 178 488 382 126 60
Cuts at: 3873 4203 4218 4352 4376 5118 5203 6274 1391
Size: 330 15 134 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 793 742 488 399 382 330
178 134 126 85 60 56 24 15
BspEI T'CCGG_A
Cuts at: 2520 6720 2520
Size: 4200 2857
BspHI T'CATG_A
Cuts at: 4946 6676 4946
Size: 1730 5327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3477 3561 4412 844
Size: 2633 84 851 3489
Fragments arranged by size:
3489 2633 851 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3269 3379 4944 1394
Size: 429 1446 110 1565 3507
Fragments arranged by size:
3507 1565 1446 429 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 5552 5726 65
Size: 1183 4304 174 1396
Fragments arranged by size:
4304 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5701 5888 1700
Size: 110 3891 187 2869
Fragments arranged by size:
3891 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2759 96
Size: 2663 4394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7057
BssSI C'ACGA_G
Cuts at: 5117 6129 5117
Size: 1012 6045
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3577 794
Size: 2783 4274
BstBI TT'CG_AA
Cuts at: 5861 5861
Size: 7057
BstEII G'GTnAC_C
Cuts at: 1184 4127 1184
Size: 2943 4114
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 3913 2291
Size: 1622 5435
BstYI r'GATC_y
Cuts at: 1972 2068 2748 3009 3390 3724 4387 4754 5139
Size: 96 680 261 381 334 663 367 385
Cuts at: 5139 5156 6608 6694 6706 7052 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 680 663 385 381 367 346
334 261 96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2674 3198 3429 3912 513
Size: 552 939 670 524 231 483 3658
Fragments arranged by size:
3658 939 670 552 524 483 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6318
Fragments arranged by size:
6318 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 2536 2716 2764 3128 4029 4274 4573 5367
Size: 139 180 48 364 901 245 299 794
Cuts at: 5367 5387 2397
Size: 20 4067
Fragments arranged by size:
4067 901 794 364 299 245 180 139
48 20
ClaI AT'CG_AT
Cuts at: 1554 4233 1554
Size: 2679 4378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7022
DraI TTT'AAA
Cuts at: 4361 4361
Size: 7057
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 2480 6064 809
Size: 507 816 348 3584 1802
Fragments arranged by size:
3584 1802 816 507 348
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3270 3823 4273
Size: 54 1425 50 275 18 1440 553 450
Cuts at: 4273 4332 4504 5393 7022 8
Size: 59 172 889 1629 43
Fragments arranged by size:
1629 1440 1425 889 553 450 275 172
59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4273 4504 1537
Size: 275 2461 231 4090
Fragments arranged by size:
4090 2461 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 3185 3739 3912 3996 4206 4987 1353
Size: 1832 554 173 84 210 781 3423
Fragments arranged by size:
3423 1832 781 554 210 173 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5644 6018 6164 387
Size: 1064 138 4055 374 146 1280
Fragments arranged by size:
4055 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2581 4216 2581
Size: 1635 5422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2868 3239 3368 3640 3701 3889
Size: 393 452 784 371 129 272 61 188
Cuts at: 3889 3948 4057 4340 5120 5717 6504 6772 1239
Size: 59 109 283 780 597 787 268 1524
Fragments arranged by size:
1524 787 784 780 597 452 393 371
283 272 268 188 129 109 61 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3942 4283 3942
Size: 341 6716
EcoO109I rG'GnC_Cy
Cuts at: 2226 3034 3301 3302 3550 3810 3869 3870 2226
Size: 808 267 1 248 260 59 1 5413
Fragments arranged by size:
5413 808 267 260 248 59 1 1
EcoRI G'AATT_C
Cuts at: 4366 4744 4366
Size: 378 6679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 7057
FspI TGC'GCA
Cuts at: 3452 5563 3452
Size: 2111 4946
FspAI rTGC'GCAy
Cuts at: 3452 3452
Size: 7057
HaeII r_GCGC'y
Cuts at: 1678 3835 6204 1678
Size: 2157 2369 2531
Fragments arranged by size:
2531 2369 2157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2293 2325 2679
Size: 32 135 32 488 32 201 32 354
Cuts at: 2679 2711 3272 3304 3716 3748 4291 4323 5698
Size: 32 561 32 412 32 543 32 1375
Cuts at: 5698 5730 5772 5804 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1375 561 543 488 412 354 201
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2470 2872 4401 4647 669
Size: 988 281 532 402 1529 246 3079
Fragments arranged by size:
3079 1529 988 532 402 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7057
HpaI GTT'AAC
Cuts at: 4647 4647
Size: 7057
KpnI G_GTAC'C
Cuts at: 4382 4382
Size: 7057
MfeI C'AATT_G
Cuts at: 4656 4656
Size: 7057
MluI A'CGCG_T
Cuts at: 6933 6933
Size: 7057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 3573 6171 6355 1305
Size: 1035 1233 2598 184 2007
Fragments arranged by size:
2598 2007 1233 1035 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3825 4334 10
Size: 54 1425 343 1993 509 2733
Fragments arranged by size:
2733 1993 1425 509 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 2949 3797 5015 5374 5533 518
Size: 648 1731 52 848 1218 359 159 2042
Fragments arranged by size:
2042 1731 1218 848 648 359 159 52
MspA1I CmG'CkG
Cuts at: 1606 2754 3431 3478 3559 3568 3679 4473 5150
Size: 1148 677 47 81 9 111 794 677
Cuts at: 5150 6298 6543 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1148 794 677 677 245 111
81 47 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2674 3912 513
Size: 552 939 670 1238 3658
Fragments arranged by size:
3658 1238 939 670 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7057
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7057
NotI GC'GGCC_GC
Cuts at: 4273 4504 4273
Size: 231 6826
NspI r_CATG'y
Cuts at: 1132 1229 2275 2948 3853 4415 5960 1132
Size: 97 1046 673 905 562 1545 2229
Fragments arranged by size:
2229 1545 1046 905 673 562 97
PasI CC'CwG_GG
Cuts at: 1348 2024 1348
Size: 676 6381
PciI A'CATG_T
Cuts at: 2271 5956 2271
Size: 3685 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2501 3801 3821 1108
Size: 1393 1300 20 4344
Fragments arranged by size:
4344 1393 1300 20
PfoI T'CCnGG_A
Cuts at: 2724 3527 2724
Size: 803 6254
PmeI GTTT'AAAC
Cuts at: 4361 4361
Size: 7057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5126 5158 6639 6671 5126
Size: 32 1481 32 5512
Fragments arranged by size:
5512 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2226 3034 3550 2226
Size: 808 516 5733
Fragments arranged by size:
5733 808 516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7057
PsiI TTA'TAA
Cuts at: 4627 4627
Size: 7057
PspOMI G'GGCC_C
Cuts at: 3301 3809 3869 3301
Size: 508 60 6489
Fragments arranged by size:
6489 508 60
PstI C_TGCA'G
Cuts at: 830 2076 3069 3462 3483 3558 3573 3946 4227
Size: 1246 993 393 21 75 15 373 281
Cuts at: 4227 4409 830
Size: 182 3478
Fragments arranged by size:
3478 1246 993 393 373 281 182 75
21 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7057
PvuII CAG'CTG
Cuts at: 3478 3568 3679 3478
Size: 90 111 6856
Fragments arranged by size:
6856 111 90
SacI G_AGCT'C
Cuts at: 2583 4218 2583
Size: 1635 5422
SacII CC_GC'GG
Cuts at: 3432 3432
Size: 7057
SalI G'TCGA_C
Cuts at: 1936 4399 1936
Size: 2463 4594
SanDI GG'GwC_CC
Cuts at: 2226 2226
Size: 7057
SapI GCTCTTCn'nnn_
Cuts at: 3739 4206 3739
Size: 467 6590
SbfI CC_TGCA'GG
Cuts at: 4409 4409
Size: 7057
ScaI AGT'ACT
Cuts at: 5305 7017 5305
Size: 1712 5345
SfcI C'TryA_G
Cuts at: 826 1045 2072 2932 3065 3458 3479 3554 3569
Size: 219 1027 860 133 393 21 75 15
Cuts at: 3569 3942 4223 4405 5540 6221 6412 826
Size: 373 281 182 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 860 681 393 373 281
219 191 182 133 75 21 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7057
SmaI CCC'GGG
Cuts at: 1872 4384 1872
Size: 2512 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 3236 3264 3313 4108 5161
Size: 197 926 840 453 28 49 795 1053
Cuts at: 5161 6062 6324 820
Size: 901 262 1553
Fragments arranged by size:
1553 1053 926 901 840 795 453 262
197 49 28
SnaBI TAC'GTA
Cuts at: 493 4848 493
Size: 4355 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7057
SphI G_CATG'C
Cuts at: 1132 3853 4415 1132
Size: 2721 562 3774
Fragments arranged by size:
3774 2721 562
SspI AAT'ATT
Cuts at: 5 52 4981 5
Size: 47 4929 2081
Fragments arranged by size:
4929 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7057
StyI C'CwwG_G
Cuts at: 513 1065 2004 2230 2462 2595 2674 3018 3423
Size: 552 939 226 232 133 79 344 405
Cuts at: 3423 3912 4496 513
Size: 489 584 3074
Fragments arranged by size:
3074 939 584 552 489 405 344 232
226 133 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2654 2677 2710 3633 3719 5084 5101
Size: 1255 1074 23 33 923 86 1365 17
Cuts at: 5101 5254 5439 5884 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1365 1255 1074 923 445 185 153
86 33 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2759 3984 5303
Size: 274 80 33 51 1443 782 1225 1319
Cuts at: 5303 7015 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1319 1225 782 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3916 3948 7017 7049 3916
Size: 32 3069 32 3924
Fragments arranged by size:
3924 3069 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3643 1316
Size: 2327 4730
XbaI T'CTAG_A
Cuts at: 4393 4393
Size: 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3280 2011
Size: 1269 5788
XhoI C'TCGA_G
Cuts at: 1943 3264 1943
Size: 1321 5736
XmaI C'CCGG_G
Cuts at: 1870 4382 1870
Size: 2512 4545
XmnI GAAnn'nnTTC
Cuts at: 2510 4037 5186 2510
Size: 1527 1149 4381
Fragments arranged by size:
4381 1527 1149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4865 276
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SexAI SfiI SfoI SrfI SwaI