Restriction map for
pFN21AB5707
(Circular) (Six-base) MAPSORT of: pFN21AB5707.seq Check: 8004 from: 1 to: 7051
pFN21AB5707 7051 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3879 3031
Size: 848 6203
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2431 2516 4861 278
Size: 53 83 186 936 895 85 2345 2468
Fragments arranged by size:
2468 2345 936 895 186 85 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 3383 4394 1937
Size: 579 867 1011 4594
Fragments arranged by size:
4594 1011 867 579
Acc65I G'GTAC_C
Cuts at: 4372 4372
Size: 7051
AclI AA'CG_TT
Cuts at: 1563 5178 5551 1563
Size: 3615 373 3063
Fragments arranged by size:
3615 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 2667 3637 4009 4284 5114 6498 6766
Size: 752 283 970 372 275 830 1384 268
Cuts at: 6766 1632
Size: 1917
Fragments arranged by size:
1917 1384 970 830 752 372 283 275
268
AflII C'TTAA_G
Cuts at: 820 1017 2291 820
Size: 197 1274 5580
Fragments arranged by size:
5580 1274 197
AflIII A'CryG_T
Cuts at: 1203 2199 5950 6927 1203
Size: 996 3751 977 1327
Fragments arranged by size:
3751 1327 996 977
AgeI A'CCGG_T
Cuts at: 5882 5882
Size: 7051
AhdI GACnn_n'nnGTC
Cuts at: 5780 5780
Size: 7051
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7051
AlwNI CAG_nnn'CTG
Cuts at: 3850 4794 6366 3850
Size: 944 1572 4535
Fragments arranged by size:
4535 1572 944
ApaLI G'TGCA_C
Cuts at: 5108 6264 5108
Size: 1156 5895
ApoI r'AATT_y
Cuts at: 1493 2588 2879 3600 4360 4586 4738 6847 1493
Size: 1095 291 721 760 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 760 721 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5605 160
Size: 872 4573 1606
Fragments arranged by size:
4573 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
AvaI C'yCGr_G
Cuts at: 1870 1943 4376 1870
Size: 73 2433 4545
Fragments arranged by size:
4545 2433 73
BamHI G'GATC_C
Cuts at: 4381 4748 4381
Size: 367 6684
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4372 5827 618
Size: 325 217 627 2585 1455 1842
Fragments arranged by size:
2585 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2827 2954 3378 3906 4370 1391
Size: 1436 127 424 528 464 4072
Fragments arranged by size:
4072 1436 528 464 424 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3297 928
Size: 971 1398 4682
Fragments arranged by size:
4682 1398 971
BbvCI CC'TCA_GC
Cuts at: 2270 2546 3345 2270
Size: 276 799 5976
Fragments arranged by size:
5976 799 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5242 5276 1903
Size: 34 3305 34 3678
Fragments arranged by size:
3678 3305 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 4943 6153 2247
Size: 2696 1210 3145
Fragments arranged by size:
3145 2696 1210
BclI T'GATC_A
Cuts at: 1548 2034 1548
Size: 486 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5662 136
Size: 107 122 71 631 605 3990 1525
Fragments arranged by size:
3990 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3064 7046 3064
Size: 3982 3069
BlpI GC'TnA_GC
Cuts at: 2332 4468 2332
Size: 2136 4915
Bme1580I G_kGCm'C
Cuts at: 1790 5112 6268 1790
Size: 3322 1156 2573
Fragments arranged by size:
3322 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2200 4210 1318
Size: 882 2010 4159
Fragments arranged by size:
4159 2010 882
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4782 5740 442
Size: 416 53 83 399 24 3365 958 1753
Fragments arranged by size:
3365 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 3360 3903 2129
Size: 1231 543 5277
Fragments arranged by size:
5277 1231 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 3371 5711 1239
Size: 866 1266 2340 2579
Fragments arranged by size:
2579 2340 1266 866
Bpu10I CC'TnA_GC
Cuts at: 2270 2546 2583 3345 2270
Size: 276 37 762 5976
Fragments arranged by size:
5976 762 276 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2968 3404 5176 6041 6339 2968
Size: 436 1772 865 298 3680
Fragments arranged by size:
3680 1772 865 436 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2796 5714 882
Size: 617 1297 2918 2219
Fragments arranged by size:
2918 2219 1297 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 4842 493
Size: 711 2426 1212 2702
Fragments arranged by size:
2702 2426 1212 711
BsaBI GATnn'nnATC
Cuts at: 7045 7045
Size: 7051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2428 2513 3896 4858
Size: 53 83 186 936 895 85 1383 962
Cuts at: 4858 5240 5875 275
Size: 382 635 1451
Fragments arranged by size:
1451 1383 962 936 895 635 382 186
85 83 53
BsaWI w'CCGG_w
Cuts at: 3460 5484 5882 6156 6303 6714 3460
Size: 2024 398 274 147 411 3797
Fragments arranged by size:
3797 2024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2207 2237 2927 2957 3831
Size: 30 165 30 804 30 690 30 874
Cuts at: 3831 3861 1178
Size: 30 4368
Fragments arranged by size:
4368 874 804 690 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2944 3269 1186
Size: 1042 716 325 4968
Fragments arranged by size:
4968 1042 716 325
BseYI C'CCAG_C
Cuts at: 3170 6254 6750 3170
Size: 3084 496 3471
Fragments arranged by size:
3471 3084 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3857 1163
Size: 573 2121 4357
Fragments arranged by size:
4357 2121 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4501 5262 6290 1540
Size: 275 186 2500 761 1028 2301
Fragments arranged by size:
2500 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2531 2584 2861 2954 5112 5197 6268 2531
Size: 53 277 93 2158 85 1071 3314
Fragments arranged by size:
3314 2158 1071 277 93 85 53
BsmI GAATG_Cn'
Cuts at: 4328 4562 4655 4328
Size: 234 93 6724
Fragments arranged by size:
6724 234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2102 2190 4216 2102
Size: 88 2026 4937
Fragments arranged by size:
4937 2026 88
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2531 2584 2827 2861 2954 3378 3906
Size: 399 741 53 243 34 93 424 528
Cuts at: 3906 4370 5112 5197 6268 1391
Size: 464 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 528 464 424 399
243 93 85 53 34
BspEI T'CCGG_A
Cuts at: 3460 6714 3460
Size: 3254 3797
BspHI T'CATG_A
Cuts at: 3103 4158 4940 6670 3103
Size: 1055 782 1730 3484
Fragments arranged by size:
3484 1730 1055 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2528 3031 3879 4190 4406 844
Size: 1684 503 848 311 216 3489
Fragments arranged by size:
3489 1684 848 503 311 216
BsrBI CCG'CTC
Cuts at: 1394 1823 4938 1394
Size: 429 3115 3507
Fragments arranged by size:
3507 3115 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2403 4056 5546 5720 65
Size: 1183 1155 1653 1490 174 1396
Fragments arranged by size:
1653 1490 1396 1183 1155 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5695 5882 1700
Size: 110 3885 187 2869
Fragments arranged by size:
3885 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2400 96
Size: 2304 4747
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7051
BssSI C'ACGA_G
Cuts at: 2059 5111 6123 2059
Size: 3052 1012 2987
Fragments arranged by size:
3052 2987 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4148 794
Size: 3354 3697
BstBI TT'CG_AA
Cuts at: 5855 5855
Size: 7051
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7051
BstXI CCAn_nnnn'nTGG
Cuts at: 2607 4250 2607
Size: 1643 5408
BstYI r'GATC_y
Cuts at: 1972 2707 3064 3792 4381 4748 5133 5150 6602
Size: 735 357 728 589 367 385 17 1452
Cuts at: 6602 6688 6700 7046 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 728 589 385 367 357
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3384 3384
Size: 7051
BtgI C'CryG_G
Cuts at: 513 1065 2004 2532 2769 513
Size: 552 939 528 237 4795
Fragments arranged by size:
4795 939 552 528 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3141 487
Size: 683 56 1915 4397
Fragments arranged by size:
4397 1915 683 56
BtsI GCAGTG_nn'
Cuts at: 4567 5361 5381 4567
Size: 794 20 6237
Fragments arranged by size:
6237 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3078 3113 529
Size: 35 2514 35 4467
Fragments arranged by size:
4467 2514 35 35
DraI TTT'AAA
Cuts at: 4355 4355
Size: 7051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6058 809
Size: 507 4742 1802
Fragments arranged by size:
4742 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3935 4498 5387
Size: 54 1425 50 275 18 2105 563 889
Cuts at: 5387 7016 8
Size: 1629 43
Fragments arranged by size:
2105 1629 1425 889 563 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4498 1537
Size: 275 2686 4090
Fragments arranged by size:
4090 2686 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2780 2815 4132 4981 1353
Size: 1427 35 1317 849 3423
Fragments arranged by size:
3423 1427 1317 849 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5638 6012 6158 387
Size: 1064 138 4049 374 146 1280
Fragments arranged by size:
4049 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2952 2952
Size: 7051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2384 2667 3371 3637 4009 4284
Size: 393 473 279 283 704 266 372 275
Cuts at: 4284 5114 5711 6498 6766 1239
Size: 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 704 597 473 393 372
283 279 275 268 266
EcoO109I rG'GnC_Cy
Cuts at: 3221 3221
Size: 7051
EcoRI G'AATT_C
Cuts at: 4360 4738 4360
Size: 378 6673
EcoRV GAT'ATC
Cuts at: 2458 2458
Size: 7051
FspI TGC'GCA
Cuts at: 5557 5557
Size: 7051
HaeII r_GCGC'y
Cuts at: 1678 6198 1678
Size: 4520 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2207 2239 2645
Size: 32 135 32 554 32 49 32 406
Cuts at: 2645 2677 2774 2806 2927 2959 4153 4185 5692
Size: 32 97 32 121 32 1194 32 1507
Cuts at: 5692 5724 5766 5798 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1507 1194 554 406 135 121 97
49 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3571 4395 4641 669
Size: 988 281 1633 824 246 3079
Fragments arranged by size:
3079 1633 988 824 281 246
HindIII A'AGCT_T
Cuts at: 748 3432 4098 748
Size: 2684 666 3701
Fragments arranged by size:
3701 2684 666
HpaI GTT'AAC
Cuts at: 4641 4641
Size: 7051
KpnI G_GTAC'C
Cuts at: 4376 4376
Size: 7051
MfeI C'AATT_G
Cuts at: 4650 4650
Size: 7051
MluI A'CGCG_T
Cuts at: 6927 6927
Size: 7051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2729 6165 6349 1305
Size: 1424 3436 184 2007
Fragments arranged by size:
3436 2007 1424 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5229
Fragments arranged by size:
5229 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5009 5368 5527 518
Size: 648 3843 359 159 2042
Fragments arranged by size:
3843 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 2269 2840 2971 3349 3617 4467 5144
Size: 615 48 571 131 378 268 850 677
Cuts at: 5144 6292 6537 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 850 677 615 571 378 268
245 131 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 2769 513
Size: 552 939 765 4795
Fragments arranged by size:
4795 939 765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7051
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7051
NotI GC'GGCC_GC
Cuts at: 4498 4498
Size: 7051
NspI r_CATG'y
Cuts at: 1132 1229 4409 5954 1132
Size: 97 3180 1545 2229
Fragments arranged by size:
3180 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3143 1348
Size: 1795 5256
PciI A'CATG_T
Cuts at: 5950 5950
Size: 7051
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3197 1108
Size: 2089 4962
PfoI T'CCnGG_A
Cuts at: 3689 3689
Size: 7051
PmeI GTTT'AAAC
Cuts at: 4355 4355
Size: 7051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 6633 6665 5120
Size: 32 1481 32 5506
Fragments arranged by size:
5506 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7051
PsiI TTA'TAA
Cuts at: 4044 4621 4044
Size: 577 6474
PstI C_TGCA'G
Cuts at: 830 3846 4014 4403 830
Size: 3016 168 389 3478
Fragments arranged by size:
3478 3016 389 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7051
PvuII CAG'CTG
Cuts at: 2221 2269 3349 3617 2221
Size: 48 1080 268 5655
Fragments arranged by size:
5655 1080 268 48
SacI G_AGCT'C
Cuts at: 2954 2954
Size: 7051
SalI G'TCGA_C
Cuts at: 1936 4393 1936
Size: 2457 4594
SapI GCTCTTCn'nnn_
Cuts at: 2815 4132 2815
Size: 1317 5734
SbfI CC_TGCA'GG
Cuts at: 4403 4403
Size: 7051
ScaI AGT'ACT
Cuts at: 5299 7011 5299
Size: 1712 5339
SexAI A'CCwGG_T
Cuts at: 2519 2519
Size: 7051
SfcI C'TryA_G
Cuts at: 826 1045 3842 4010 4399 5534 6215 6406 826
Size: 219 2797 168 389 1135 681 191 1471
Fragments arranged by size:
2797 1471 1135 681 389 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7051
SmaI CCC'GGG
Cuts at: 1872 4378 1872
Size: 2506 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 2947 3419 5155 6056 6318
Size: 197 926 348 656 472 1736 901 262
Cuts at: 6318 820
Size: 1553
Fragments arranged by size:
1736 1553 926 901 656 472 348 262
197
SnaBI TAC'GTA
Cuts at: 493 4842 493
Size: 4349 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7051
SphI G_CATG'C
Cuts at: 1132 4409 1132
Size: 3277 3774
SspI AAT'ATT
Cuts at: 5 52 4295 4975 5
Size: 47 4243 680 2081
Fragments arranged by size:
4243 2081 680 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7051
StyI C'CwwG_G
Cuts at: 513 1065 2004 2769 4490 513
Size: 552 939 765 1721 3074
Fragments arranged by size:
3074 1721 939 765 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3681 3850 5078 5095 5248 5433 5878
Size: 1255 2101 169 1228 17 153 185 445
Cuts at: 5878 325
Size: 1498
Fragments arranged by size:
2101 1498 1255 1228 445 185 169 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2565 4310
Size: 274 80 33 51 1443 423 165 1745
Cuts at: 4310 5297 7009 96
Size: 987 1712 138
Fragments arranged by size:
1745 1712 1443 987 423 274 165 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7011 7043 7011
Size: 32 7019
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7051
XbaI T'CTAG_A
Cuts at: 4387 4387
Size: 7051
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7051
XmaI C'CCGG_G
Cuts at: 1870 4376 1870
Size: 2506 4545
XmnI GAAnn'nnTTC
Cuts at: 5180 5180
Size: 7051
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2429 2514 4859 276
Size: 53 83 186 936 895 85 2345 2468
Fragments arranged by size:
2468 2345 936 895 186 85 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI Bsu36I DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI