Restriction map for
pFN21AB5706
(Circular) (Six-base) MAPSORT of: pFN21AB5706.seq Check: 2111 from: 1 to: 6856
pFN21AB5706 6856 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3505 4199 1937
Size: 1568 694 4594
Fragments arranged by size:
4594 1568 694
Acc65I G'GTAC_C
Cuts at: 4177 4177
Size: 6856
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4919 6303 6571 1632
Size: 3287 1384 268 1917
Fragments arranged by size:
3287 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 2656 5755 6732 1203
Size: 1453 3099 977 1327
Fragments arranged by size:
3099 1453 1327 977
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 5585 5585
Size: 6856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6856
AlwNI CAG_nnn'CTG
Cuts at: 2205 2854 4599 6171 2205
Size: 649 1745 1572 2890
Fragments arranged by size:
2890 1745 1572 649
ApaI G_GGCC'C
Cuts at: 3463 3994 3463
Size: 531 6325
ApaLI G'TGCA_C
Cuts at: 4913 6069 4913
Size: 1156 5700
ApoI r'AATT_y
Cuts at: 1493 3152 3927 4165 4391 4543 6652 1493
Size: 1659 775 238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1659 775 238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 3393 4181 1870
Size: 73 1450 788 4545
Fragments arranged by size:
4545 1450 788 73
BamHI G'GATC_C
Cuts at: 4186 4553 4186
Size: 367 6489
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 4177 5632 618
Size: 325 217 627 926 1464 1455 1842
Fragments arranged by size:
1842 1464 1455 926 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3463 3994 4175 1391
Size: 2072 531 181 4072
Fragments arranged by size:
4072 2072 531 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 928
Size: 971 266 5619
Fragments arranged by size:
5619 971 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 1903
Size: 34 3110 34 3678
Fragments arranged by size:
3678 3110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4748 5958 4748
Size: 1210 5646
BclI T'GATC_A
Cuts at: 1548 2544 1548
Size: 996 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5467 136
Size: 107 122 71 631 605 3795 1525
Fragments arranged by size:
3795 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6851 6851
Size: 6856
BlpI GC'TnA_GC
Cuts at: 3660 4273 3660
Size: 613 6243
Bme1580I G_kGCm'C
Cuts at: 1790 3463 3994 4917 6073 1790
Size: 1673 531 923 1156 2573
Fragments arranged by size:
2573 1673 1156 923 531
BmgBI CAC'GTC
Cuts at: 1318 2657 1318
Size: 1339 5517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 2184 2893
Size: 416 53 83 399 24 759 8 709
Cuts at: 2893 3680 3830 4587 5545 442
Size: 787 150 757 958 1753
Fragments arranged by size:
1753 958 787 759 757 709 416 399
150 83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 3668 4004 1056
Size: 2612 336 3908
Fragments arranged by size:
3908 2612 336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2808 2808
Size: 6856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 3468 3786 5516 1239
Size: 1345 884 318 1730 2579
Fragments arranged by size:
2579 1730 1345 884 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2358 2718 2927 4981 5846 6144 2358
Size: 360 209 2054 865 298 3070
Fragments arranged by size:
3070 2054 865 360 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5519 882
Size: 617 4020 2219
Fragments arranged by size:
4020 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4647 493
Size: 711 3443 2702
Fragments arranged by size:
3443 2702 711
BsaBI GATnn'nnATC
Cuts at: 3603 6850 3603
Size: 3247 3609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4663 5045 5680 275
Size: 53 83 186 936 3130 382 635 1451
Fragments arranged by size:
3130 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3679 5289 5687 5961 6108 6519 3679
Size: 1610 398 274 147 411 4016
Fragments arranged by size:
4016 1610 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4089 4119 1178
Size: 30 165 30 2686 30 3915
Fragments arranged by size:
3915 2686 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2709 2721 3867 1186
Size: 1523 12 1146 4175
Fragments arranged by size:
4175 1523 1146 12
BseYI C'CCAG_C
Cuts at: 2863 3307 6059 6555 2863
Size: 444 2752 496 3164
Fragments arranged by size:
3164 2752 496 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6283
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4306 5067 6095 1540
Size: 275 186 2305 761 1028 2301
Fragments arranged by size:
2305 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4135 4917 5002 6073 4135
Size: 782 85 1071 4918
Fragments arranged by size:
4918 1071 782 85
BsmI GAATG_Cn'
Cuts at: 2112 2653 3536 4367 4460 2112
Size: 541 883 831 93 4508
Fragments arranged by size:
4508 883 831 541 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2007 2007
Size: 6856
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3463 3994 4135 4175 4917 5002 6073
Size: 399 1673 531 141 40 742 85 1071
Cuts at: 6073 1391
Size: 2174
Fragments arranged by size:
2174 1673 1071 742 531 399 141 85
40
BspEI T'CCGG_A
Cuts at: 3679 6519 3679
Size: 2840 4016
BspHI T'CATG_A
Cuts at: 4117 4745 6475 4117
Size: 628 1730 4498
Fragments arranged by size:
4498 1730 628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4211 844
Size: 3367 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4743 1394
Size: 429 2920 3507
Fragments arranged by size:
3507 2920 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5351 5525 65
Size: 1183 4103 174 1396
Fragments arranged by size:
4103 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5500 5687 1700
Size: 110 3690 187 2869
Fragments arranged by size:
3690 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2214 3904 96
Size: 2118 1690 3048
Fragments arranged by size:
3048 2118 1690
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 4916 5928 4916
Size: 1012 5844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6856
BstBI TT'CG_AA
Cuts at: 5660 5660
Size: 6856
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstYI r'GATC_y
Cuts at: 1972 2474 4186 4553 4938 4955 6407 6493 6505
Size: 502 1712 367 385 17 1452 86 12
Cuts at: 6505 6851 1972
Size: 346 1977
Fragments arranged by size:
1977 1712 1452 502 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3506 3506
Size: 6856
BtgI C'CryG_G
Cuts at: 513 1065 2022 4023 513
Size: 552 957 2001 3346
Fragments arranged by size:
3346 2001 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6117
Fragments arranged by size:
6117 683 56
BtsI GCAGTG_nn'
Cuts at: 4372 5166 5186 4372
Size: 794 20 6042
Fragments arranged by size:
6042 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6821
DraI TTT'AAA
Cuts at: 3199 4160 3199
Size: 961 5895
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 809
Size: 507 4547 1802
Fragments arranged by size:
4547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4303 5192 6821
Size: 54 1425 50 275 18 2473 889 1629
Cuts at: 6821 8
Size: 43
Fragments arranged by size:
2473 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4303 1537
Size: 275 2491 4090
Fragments arranged by size:
4090 2491 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2506 2580 2799 3048 3540 4786 1353
Size: 723 430 74 219 249 492 1246 3423
Fragments arranged by size:
3423 1246 723 492 430 249 219 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5443 5817 5963 387
Size: 1064 138 3854 374 146 1280
Fragments arranged by size:
3854 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2584 3468 3786 4919 5516 6303 6571
Size: 393 952 884 318 1133 597 787 268
Cuts at: 6571 1239
Size: 1524
Fragments arranged by size:
1524 1133 952 884 787 597 393 318
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 3377 2282
Size: 1095 5761
EcoO109I rG'GnC_Cy
Cuts at: 3896 3990 3991 3896
Size: 94 1 6761
Fragments arranged by size:
6761 94 1
EcoRI G'AATT_C
Cuts at: 4165 4543 4165
Size: 378 6478
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 6003 1678
Size: 4325 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2611 2643 4089 4121 5497
Size: 32 135 32 1039 32 1446 32 1376
Cuts at: 5497 5529 5571 5603 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 1376 1039 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4200 4446 669
Size: 988 281 2262 246 3079
Fragments arranged by size:
3079 2262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6856
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KpnI G_GTAC'C
Cuts at: 4181 4181
Size: 6856
MfeI C'AATT_G
Cuts at: 3108 4455 3108
Size: 1347 5509
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5970 6154 1305
Size: 4665 184 2007
Fragments arranged by size:
4665 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5034
Fragments arranged by size:
5034 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3237 3375 4814 5173 5332 518
Size: 648 2071 138 1439 359 159 2042
Fragments arranged by size:
2071 2042 1439 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2036 2504 3851 4272 4949 6097 6342 1606
Size: 430 468 1347 421 677 1148 245 2120
Fragments arranged by size:
2120 1347 1148 677 468 430 421 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6304
NdeI CA'TA_TG
Cuts at: 387 2092 2323 2380 3040 387
Size: 1705 231 57 660 4203
Fragments arranged by size:
4203 1705 660 231 57
NheI G'CTAG_C
Cuts at: 1052 3664 4000 1052
Size: 2612 336 3908
Fragments arranged by size:
3908 2612 336
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 4214 5759 1132
Size: 97 2985 1545 2229
Fragments arranged by size:
2985 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6856
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6856
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3896 3896
Size: 6856
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 2819 3642 4426 2819
Size: 823 784 5249
Fragments arranged by size:
5249 823 784
PspOMI G'GGCC_C
Cuts at: 3459 3990 3459
Size: 531 6325
PstI C_TGCA'G
Cuts at: 830 3250 3850 4208 830
Size: 2420 600 358 3478
Fragments arranged by size:
3478 2420 600 358
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 2036 2504 3851 2036
Size: 468 1347 5041
Fragments arranged by size:
5041 1347 468
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SbfI CC_TGCA'GG
Cuts at: 4208 4208
Size: 6856
ScaI AGT'ACT
Cuts at: 4041 5104 6816 4041
Size: 1063 1712 4081
Fragments arranged by size:
4081 1712 1063
SexAI A'CCwGG_T
Cuts at: 3820 3820
Size: 6856
SfcI C'TryA_G
Cuts at: 826 1045 2142 2469 3246 3268 3702 3846 4005
Size: 219 1097 327 777 22 434 144 159
Cuts at: 4005 4204 5339 6020 6211 826
Size: 199 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 777 681 434 327 219
199 191 159 144 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 4183 1872
Size: 2311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2373 2733 2906 4960 5861 6123
Size: 197 926 430 360 173 2054 901 262
Cuts at: 6123 820
Size: 1553
Fragments arranged by size:
2054 1553 926 901 430 360 262 197
173
SnaBI TAC'GTA
Cuts at: 493 4647 493
Size: 4154 2702
SpeI A'CTAG_T
Cuts at: 152 3574 152
Size: 3422 3434
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 2599 3263 3487 4780 5
Size: 47 2547 664 224 1293 2081
Fragments arranged by size:
2547 2081 1293 664 224 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6856
StyI C'CwwG_G
Cuts at: 513 1065 2337 3129 4047 4295 513
Size: 552 1272 792 918 248 3074
Fragments arranged by size:
3074 1272 918 792 552 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 4883 4900 5053 5238 5683 325
Size: 1255 842 2461 17 153 185 445 1498
Fragments arranged by size:
2461 1498 1255 842 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 3904 4039
Size: 274 80 33 51 1443 237 1690 135
Cuts at: 4039 5102 6814 96
Size: 1063 1712 138
Fragments arranged by size:
1712 1690 1443 1063 274 237 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6816 6848 6816
Size: 32 6824
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6856
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6856
XmaI C'CCGG_G
Cuts at: 1870 4181 1870
Size: 2311 4545
XmnI GAAnn'nnTTC
Cuts at: 3078 4985 3078
Size: 1907 4949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI