Restriction map for
pFN21AB5705
(Circular) (Six-base) MAPSORT of: pFN21AB5705.seq Check: 3362 from: 1 to: 6937
pFN21AB5705 6937 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2201 4747 278
Size: 53 83 186 936 665 2546 2468
Fragments arranged by size:
2546 2468 936 665 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3753 3780 4280 1937
Size: 1816 27 500 4594
Fragments arranged by size:
4594 1816 500 27
Acc65I G'GTAC_C
Cuts at: 2088 4258 2088
Size: 2170 4767
AclI AA'CG_TT
Cuts at: 1563 5064 5437 1563
Size: 3501 373 3063
Fragments arranged by size:
3501 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5000 6384 6652 1632
Size: 3368 1384 268 1917
Fragments arranged by size:
3368 1917 1384 268
AfeI AGC'GCT
Cuts at: 3082 3082
Size: 6937
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6740
AflIII A'CryG_T
Cuts at: 1203 5836 6813 1203
Size: 4633 977 1327
Fragments arranged by size:
4633 1327 977
AgeI A'CCGG_T
Cuts at: 5768 5768
Size: 6937
AhdI GACnn_n'nnGTC
Cuts at: 3787 5666 3787
Size: 1879 5058
AleI CACnn'nnGTG
Cuts at: 1166 3617 1166
Size: 2451 4486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2468 2840 3353 4029 1184
Size: 1284 372 513 676 4092
Fragments arranged by size:
4092 1284 676 513 372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2042
Size: 32 6905
AlwNI CAG_nnn'CTG
Cuts at: 2062 2076 2167 2572 2659 2757 2779 4159 4680
Size: 14 91 405 87 98 22 1380 521
Cuts at: 4680 6252 2062
Size: 1572 2747
Fragments arranged by size:
2747 1572 1380 521 405 98 91 87
22 14
ApaI G_GGCC'C
Cuts at: 2293 3737 3879 2293
Size: 1444 142 5351
Fragments arranged by size:
5351 1444 142
ApaLI G'TGCA_C
Cuts at: 4994 6150 4994
Size: 1156 5781
ApoI r'AATT_y
Cuts at: 1493 3458 4246 4472 4624 6733 1493
Size: 1965 788 226 152 2109 1697
Fragments arranged by size:
2109 1965 1697 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5491 160
Size: 872 4459 1606
Fragments arranged by size:
4459 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 4262 1870
Size: 73 558 1761 4545
Fragments arranged by size:
4545 1761 558 73
BamHI G'GATC_C
Cuts at: 4267 4634 4267
Size: 367 6570
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2278 3130 4258 5713
Size: 325 217 627 301 190 852 1128 1455
Cuts at: 5713 618
Size: 1842
Fragments arranged by size:
1842 1455 1128 852 627 325 301 217
190
BanII G_rGCy'C
Cuts at: 1391 2293 2792 3420 3737 3879 4256 1391
Size: 902 499 628 317 142 377 4072
Fragments arranged by size:
4072 902 628 499 377 317 142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3415 928
Size: 971 1516 4450
Fragments arranged by size:
4450 1516 971
BbvCI CC'TCA_GC
Cuts at: 2456 2456
Size: 6937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4152 4186 5128 5162 1903
Size: 34 2215 34 942 34 3678
Fragments arranged by size:
3678 2215 942 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4829 6039 4829
Size: 1210 5727
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6937
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3114 3732 5548
Size: 107 122 71 631 605 1442 618 1816
Cuts at: 5548 136
Size: 1525
Fragments arranged by size:
1816 1525 1442 631 618 605 122 107
71
BglII A'GATC_T
Cuts at: 6932 6932
Size: 6937
BlpI GC'TnA_GC
Cuts at: 3084 4354 3084
Size: 1270 5667
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2293 3259 3737 3879 4998 6154 1790
Size: 309 194 966 478 142 1119 1156 2573
Fragments arranged by size:
2573 1156 1119 966 478 309 194 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6937
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4075 4668 5626
Size: 416 53 83 399 24 2658 593 958
Cuts at: 5626 442
Size: 1753
Fragments arranged by size:
2658 1753 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6937
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 3986
Size: 6937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 2693 4217 5597 1239
Size: 1409 45 1524 1380 2579
Fragments arranged by size:
2579 1524 1409 1380 45
Bpu10I CC'TnA_GC
Cuts at: 2456 2456
Size: 6937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 5062 5927 6225 2924
Size: 2138 865 298 3636
Fragments arranged by size:
3636 2138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 5600 882
Size: 617 1170 2931 2219
Fragments arranged by size:
2931 2219 1170 617
BsaAI yAC'GTr
Cuts at: 493 1204 4728 493
Size: 711 3524 2702
Fragments arranged by size:
3524 2702 711
BsaBI GATnn'nnATC
Cuts at: 6931 6931
Size: 6937
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2198 2220 4744 5126
Size: 53 83 186 936 665 22 2524 382
Cuts at: 5126 5761 275
Size: 635 1451
Fragments arranged by size:
2524 1451 936 665 635 382 186 83
53 22
BsaWI w'CCGG_w
Cuts at: 3127 3756 5370 5768 6042 6189 6600 3127
Size: 629 1614 398 274 147 411 3464
Fragments arranged by size:
3464 1614 629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3735 3765 4017 4047 1178
Size: 30 165 30 2332 30 252 30 4068
Fragments arranged by size:
4068 2332 252 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3191 1186
Size: 2005 4932
BseYI C'CCAG_C
Cuts at: 3362 6140 6636 3362
Size: 2778 496 3663
Fragments arranged by size:
3663 2778 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2631 2952 3114 1163
Size: 573 366 529 321 162 4986
Fragments arranged by size:
4986 573 529 366 321 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2927 3499 4387 5148 6176 1540
Size: 275 186 926 572 888 761 1028 2301
Fragments arranged by size:
2301 1028 926 888 761 572 275 186
BsiHKAI G_wGCw'C
Cuts at: 2934 3420 4998 5083 6154 2934
Size: 486 1578 85 1071 3717
Fragments arranged by size:
3717 1578 1071 486 85
BsmI GAATG_Cn'
Cuts at: 2887 4448 4541 2887
Size: 1561 93 5283
Fragments arranged by size:
5283 1561 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2227 2390 2227
Size: 163 6774
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2293 2792 2934 3259 3420 3737
Size: 399 309 194 499 142 325 161 317
Cuts at: 3737 3879 4256 4998 5083 6154 1391
Size: 142 377 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 377 325 317
309 194 161 142 142 85
BspEI T'CCGG_A
Cuts at: 6600 6600
Size: 6937
BspHI T'CATG_A
Cuts at: 4826 6556 4826
Size: 1730 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3282 4292 844
Size: 2438 1010 3489
Fragments arranged by size:
3489 2438 1010
BsrBI CCG'CTC
Cuts at: 1394 1823 2833 2869 4824 1394
Size: 429 1010 36 1955 3507
Fragments arranged by size:
3507 1955 1010 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 4006 5432 5606 65
Size: 1183 1026 1732 1426 174 1396
Fragments arranged by size:
1732 1426 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 3104 5581 5768 1700
Size: 110 380 914 2477 187 2869
Fragments arranged by size:
2869 2477 914 380 187 110
BsrGI T'GTAC_A
Cuts at: 96 3171 3372 3948 96
Size: 3075 201 576 3085
Fragments arranged by size:
3085 3075 576 201
BssHII G'CGCG_C
Cuts at: 1925 3179 1925
Size: 1254 5683
BssSI C'ACGA_G
Cuts at: 2956 4997 6009 2956
Size: 2041 1012 3884
Fragments arranged by size:
3884 2041 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2076 2543 2638 2757 2779 4209 794
Size: 1282 467 95 119 22 1430 3522
Fragments arranged by size:
3522 1430 1282 467 119 95 22
BstBI TT'CG_AA
Cuts at: 5741 5741
Size: 6937
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6937
BstXI CCAn_nnnn'nTGG
Cuts at: 3152 3619 3745 3818 3152
Size: 467 126 73 6271
Fragments arranged by size:
6271 467 126 73
BstYI r'GATC_y
Cuts at: 1972 4267 4634 5019 5036 6488 6574 6586 6932
Size: 2295 367 385 17 1452 86 12 346
Cuts at: 6932 1972
Size: 1977
Fragments arranged by size:
2295 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3690 3690
Size: 6937
BtgI C'CryG_G
Cuts at: 513 1065 2004 2985 3847 3988 513
Size: 552 939 981 862 141 3462
Fragments arranged by size:
3462 981 939 862 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6198
Fragments arranged by size:
6198 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 2636 2723 2777 2945 2964 3357 3630 4207
Size: 408 87 54 168 19 393 273 577
Cuts at: 4207 4453 5247 5267 2228
Size: 246 794 20 3898
Fragments arranged by size:
3898 794 577 408 393 273 246 168
87 54 20 19
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6902
DraI TTT'AAA
Cuts at: 4216 4241 4216
Size: 25 6912
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5944 809
Size: 507 4628 1802
Fragments arranged by size:
4628 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 2400 2515
Size: 54 1425 50 275 18 502 68 115
Cuts at: 2515 2870 2982 3496 3721 3747 3832 4177 4384
Size: 355 112 514 225 26 85 345 207
Cuts at: 4384 5273 6902 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 514 502 355 345 275
225 207 115 112 85 68 54 50
43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 4384 1537
Size: 275 1684 888 4090
Fragments arranged by size:
4090 1684 888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3977 4867 1353
Size: 2624 890 3423
Fragments arranged by size:
3423 2624 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5524 5898 6044 387
Size: 1064 138 3935 374 146 1280
Fragments arranged by size:
3935 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3418 3418
Size: 6937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 2693 4217 5000 5597 6384 6652
Size: 393 1016 45 1524 783 597 787 268
Cuts at: 6652 1239
Size: 1524
Fragments arranged by size:
1524 1524 1016 787 783 597 393 268
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 3002 2261
Size: 741 6196
EcoO109I rG'GnC_Cy
Cuts at: 2289 2305 2593 2623 2995 3271 3734 3875 3876
Size: 16 288 30 372 276 463 141 1
Cuts at: 3876 4156 2289
Size: 280 5070
Fragments arranged by size:
5070 463 372 288 280 276 141 30
16 1
EcoRI G'AATT_C
Cuts at: 3458 4246 4624 3458
Size: 788 378 5771
Fragments arranged by size:
5771 788 378
FspI TGC'GCA
Cuts at: 2816 3071 5443 2816
Size: 255 2372 4310
Fragments arranged by size:
4310 2372 255
FspAI rTGC'GCAy
Cuts at: 3071 3071
Size: 6937
HaeII r_GCGC'y
Cuts at: 1678 2497 2964 3011 3084 3304 3610 6084 1678
Size: 819 467 47 73 220 306 2474 2531
Fragments arranged by size:
2531 2474 819 467 306 220 73 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2916 2948 3773 3805 5578
Size: 32 135 32 1344 32 825 32 1773
Cuts at: 5578 5610 5652 5684 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1773 1344 825 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3781 4281 4527 669
Size: 988 281 1843 500 246 3079
Fragments arranged by size:
3079 1843 988 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6937
HpaI GTT'AAC
Cuts at: 4527 4527
Size: 6937
KpnI G_GTAC'C
Cuts at: 2092 4262 2092
Size: 2170 4767
MfeI C'AATT_G
Cuts at: 2541 3405 4536 2541
Size: 864 1131 4942
Fragments arranged by size:
4942 1131 864
MluI A'CGCG_T
Cuts at: 6813 6813
Size: 6937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6051 6235 1305
Size: 4746 184 2007
Fragments arranged by size:
4746 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2984 4179 10
Size: 54 1425 343 1152 1195 2768
Fragments arranged by size:
2768 1425 1195 1152 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2880 3150 3617 3647 4055 4895 5254
Size: 648 1714 270 467 30 408 840 359
Cuts at: 5254 5413 518
Size: 159 2042
Fragments arranged by size:
2042 1714 840 648 467 408 359 270
159 30
MspA1I CmG'CkG
Cuts at: 1606 2530 2572 2716 2890 3495 3601 4353 5030
Size: 924 42 144 174 605 106 752 677
Cuts at: 5030 6178 6423 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 924 752 677 605 245 174
144 106 42
NaeI GCC'GGC
Cuts at: 2192 2192
Size: 6937
NcoI C'CATG_G
Cuts at: 513 1065 2004 3988 513
Size: 552 939 1984 3462
Fragments arranged by size:
3462 1984 939 552
NdeI CA'TA_TG
Cuts at: 387 3160 387
Size: 2773 4164
NgoMIV G'CCGG_C
Cuts at: 2190 2190
Size: 6937
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6937
NotI GC'GGCC_GC
Cuts at: 3496 4384 3496
Size: 888 6049
NspI r_CATG'y
Cuts at: 1132 1229 2827 4295 5840 1132
Size: 97 1598 1468 1545 2229
Fragments arranged by size:
2229 1598 1545 1468 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6937
PciI A'CATG_T
Cuts at: 5836 5836
Size: 6937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 3886 4145 4186 1108
Size: 939 1839 259 41 3859
Fragments arranged by size:
3859 1839 939 259 41
PfoI T'CCnGG_A
Cuts at: 2862 2862
Size: 6937
PmeI GTTT'AAAC
Cuts at: 4241 4241
Size: 6937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3683 3715 5006 5038 6519 6551 3542
Size: 32 109 32 1291 32 1481 32 3928
Fragments arranged by size:
3928 1481 1291 109 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2623 3271 2305
Size: 318 648 5971
Fragments arranged by size:
5971 648 318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6937
PsiI TTA'TAA
Cuts at: 4507 4507
Size: 6937
PspOMI G'GGCC_C
Cuts at: 2289 3733 3875 2289
Size: 1444 142 5351
Fragments arranged by size:
5351 1444 142
PspXI vC'TCGA_Gb
Cuts at: 2501 2501
Size: 6937
PstI C_TGCA'G
Cuts at: 830 2235 2493 2577 2643 2762 3606 4135 4289
Size: 1405 258 84 66 119 844 529 154
Cuts at: 4289 830
Size: 3478
Fragments arranged by size:
3478 1405 844 529 258 154 119 84
66
PvuI CG_AT'CG
Cuts at: 2001 2927 2001
Size: 926 6011
PvuII CAG'CTG
Cuts at: 2530 2572 2716 2890 3601 2530
Size: 42 144 174 711 5866
Fragments arranged by size:
5866 711 174 144 42
SacI G_AGCT'C
Cuts at: 3420 3420
Size: 6937
SalI G'TCGA_C
Cuts at: 1936 3779 4279 1936
Size: 1843 500 4594
Fragments arranged by size:
4594 1843 500
SbfI CC_TGCA'GG
Cuts at: 4289 4289
Size: 6937
ScaI AGT'ACT
Cuts at: 5185 6897 5185
Size: 1712 5225
SfcI C'TryA_G
Cuts at: 826 1045 2231 2489 2573 2639 2758 3602 4131
Size: 219 1186 258 84 66 119 844 529
Cuts at: 4131 4285 5420 6101 6292 826
Size: 154 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 844 681 529 258 219
191 154 119 84 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6937
SmaI CCC'GGG
Cuts at: 1872 4264 1872
Size: 2392 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2903 5041 5942 6204 820
Size: 197 926 558 402 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 558 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4728 493
Size: 4235 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6937
SphI G_CATG'C
Cuts at: 1132 4295 1132
Size: 3163 3774
SspI AAT'ATT
Cuts at: 5 52 4861 5
Size: 47 4809 2081
Fragments arranged by size:
4809 2081 47
StuI AGG'CCT
Cuts at: 1517 2390 2858 1517
Size: 873 468 5596
Fragments arranged by size:
5596 873 468
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 3738 3988 4376 513
Size: 552 939 96 1638 250 388 3074
Fragments arranged by size:
3074 1638 939 552 388 250 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4964 4981 5134 5319 5764 325
Size: 1255 3384 17 153 185 445 1498
Fragments arranged by size:
3384 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3171 3372 3948
Size: 274 80 33 51 1443 1194 201 576
Cuts at: 3948 5183 6895 96
Size: 1235 1712 138
Fragments arranged by size:
1712 1443 1235 1194 576 274 201 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 3109 3141 3213 3245 4017 4049 6897
Size: 32 233 32 72 32 772 32 2848
Cuts at: 6897 6929 2844
Size: 32 2852
Fragments arranged by size:
2852 2848 772 233 72 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6937
XbaI T'CTAG_A
Cuts at: 4273 4273
Size: 6937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3823 3823
Size: 6937
XhoI C'TCGA_G
Cuts at: 1943 2501 1943
Size: 558 6379
XmaI C'CCGG_G
Cuts at: 1870 4262 1870
Size: 2392 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 5066 2240
Size: 2826 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2199 4745 276
Size: 53 83 186 936 665 2546 2468
Fragments arranged by size:
2546 2468 936 665 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI KasI NarI NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI