Restriction map for
pFN21AB5700
(Circular) (Six-base) MAPSORT of: pFN21AB5700.seq Check: 7034 from: 1 to: 5983
pFN21AB5700 5983 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3066 4046 5430 5698 1632
Size: 1434 980 1384 268 1917
Fragments arranged by size:
1917 1434 1384 980 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AlwNI CAG_nnn'CTG
Cuts at: 3726 5298 3726
Size: 1572 4411
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2156 2691 2891 3292 3518 3670 5779 1493
Size: 663 535 200 401 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 535 401 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
AvrII C'CTAG_G
Cuts at: 2731 2731
Size: 5983
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3304 4759 618
Size: 325 217 627 1517 1455 1842
Fragments arranged by size:
1842 1517 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3302 1391
Size: 1911 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 928
Size: 971 186 4826
Fragments arranged by size:
4826 971 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4174 4208 1903
Size: 34 50 34 2153 34 3678
Fragments arranged by size:
3678 2153 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 2868 1548
Size: 1320 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2704 5978 2704
Size: 3274 2709
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 4044 5200 1790
Size: 2254 1156 2573
Fragments arranged by size:
2573 2254 1156
BmgBI CAC'GTC
Cuts at: 1318 2177 1318
Size: 859 5124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2622 3138 3249 4643 1239
Size: 1166 217 516 111 1394 2579
Fragments arranged by size:
2579 1394 1166 516 217 111
Bpu10I CC'TnA_GC
Cuts at: 2025 2025
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 3216 5977 3216
Size: 2761 3222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5758
Fragments arranged by size:
5758 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5983
BseYI C'CCAG_C
Cuts at: 5186 5682 5186
Size: 496 5487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 1163
Size: 573 580 4830
Fragments arranged by size:
4830 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4044 4129 5200 4044
Size: 85 1071 4827
Fragments arranged by size:
4827 1071 85
BsmI GAATG_Cn'
Cuts at: 2321 3494 3587 2321
Size: 1173 93 4717
Fragments arranged by size:
4717 1173 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3302 4044 4129 5200 1391
Size: 399 1512 742 85 1071 2174
Fragments arranged by size:
2174 1512 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 3012 96
Size: 2916 3067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2299 2704 3313 3680 4065 4082 5534 5620
Size: 327 405 609 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 609 405 385 367 346 327
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 2192 2192
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948
Size: 54 1425 50 275 18 1600 889 1629
Cuts at: 5948 8
Size: 43
Fragments arranged by size:
1629 1600 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3150 3913 1353
Size: 1317 480 763 3423
Fragments arranged by size:
3423 1317 763 480
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2525 4570 4944 5090 387
Size: 1064 138 474 462 2045 374 146 1280
Fragments arranged by size:
2045 1280 1064 474 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2622 3066 3138 3249 4046 4643
Size: 393 773 217 444 72 111 797 597
Cuts at: 4643 5430 5698 1239
Size: 787 268 1524
Fragments arranged by size:
1524 797 787 773 597 444 393 268
217 111 72
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3129 3129
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 5130 1678
Size: 3452 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 2860 2892 4624
Size: 32 135 32 719 32 537 32 1732
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1732 719 537 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2165 3327 3573 669
Size: 988 281 227 1162 246 3079
Fragments arranged by size:
3079 1162 988 281 246 227
HindIII A'AGCT_T
Cuts at: 748 3111 748
Size: 2363 3620
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3941 4300 4459 518
Size: 648 2775 359 159 2042
Fragments arranged by size:
2775 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3399 4076 5224 5469 1606
Size: 1793 677 1148 245 2120
Fragments arranged by size:
2120 1793 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2623 3341 4886 1132
Size: 97 1394 718 1545 2229
Fragments arranged by size:
2229 1545 1394 718 97
PasI CC'CwG_GG
Cuts at: 1348 2950 1348
Size: 1602 4381
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PfoI T'CCnGG_A
Cuts at: 2553 2641 2553
Size: 88 5895
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2739 4052 4084 5565 5597 2707
Size: 32 1313 32 1481 32 3093
Fragments arranged by size:
3093 1481 1313 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2265 2310 3048 3331 4466 5147 5338
Size: 219 1220 45 738 283 1135 681 191
Cuts at: 5338 826
Size: 1471
Fragments arranged by size:
1471 1220 1135 738 681 283 219 191
45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4087 4988 5250 820
Size: 197 926 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 2863 152
Size: 2711 3272
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2731 3422 513
Size: 552 1666 691 3074
Fragments arranged by size:
3074 1666 691 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 4010 4027 4180 4365 4810 325
Size: 1255 1383 1047 17 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 1047 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2342 3012 4229
Size: 274 80 33 51 1443 365 670 1217
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1217 670 365 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5943 5975 5943
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 2758 3319 2758
Size: 561 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2957 2957
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2766 4112 2766
Size: 1346 4637
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI