Restriction map for
pFN21AB5690
(Circular) (Six-base) MAPSORT of: pFN21AB5690.seq Check: 8078 from: 1 to: 6259
pFN21AB5690 6259 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3305 3305
Size: 6259
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2439 4069 278
Size: 53 83 186 936 903 1630 2468
Fragments arranged by size:
2468 1630 936 903 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2247 2798 3602 1937
Size: 310 551 804 4594
Fragments arranged by size:
4594 804 551 310
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 2682 4386 4759 1563
Size: 1119 1704 373 3063
Fragments arranged by size:
3063 1704 1119 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4322 5706 5974 1632
Size: 2690 1384 268 1917
Fragments arranged by size:
2690 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 2385 5158 6135 1203
Size: 1182 2773 977 1327
Fragments arranged by size:
2773 1327 1182 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2942 1184
Size: 1758 4501
AlwNI CAG_nnn'CTG
Cuts at: 3320 3329 3372 4002 5574 3320
Size: 9 43 630 1572 4005
Fragments arranged by size:
4005 1572 630 43 9
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 2557 2835 2996 3043 3333 3568 3794 3946
Size: 1064 278 161 47 290 235 226 152
Cuts at: 3946 6055 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1064 290 278 235 226 161
152 47
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2143 2150 3584 1870
Size: 73 76 124 7 1434 4545
Fragments arranged by size:
4545 1434 124 76 73 7
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2918 3227 3580 5035
Size: 325 217 627 435 696 309 353 1455
Cuts at: 5035 618
Size: 1842
Fragments arranged by size:
1842 1455 696 627 435 353 325 309
217
BanII G_rGCy'C
Cuts at: 1391 3271 3299 3402 3578 1391
Size: 1880 28 103 176 4072
Fragments arranged by size:
4072 1880 176 103 28
BbeI G_GCGC'C
Cuts at: 2226 2226
Size: 6259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 928
Size: 971 591 4697
Fragments arranged by size:
4697 971 591
BbvCI CC'TCA_GC
Cuts at: 3135 3135
Size: 6259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4450 4484 1903
Size: 34 2513 34 3678
Fragments arranged by size:
3678 2513 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4870 136
Size: 107 122 71 631 605 3198 1525
Fragments arranged by size:
3198 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 4320 5476 1790
Size: 2530 1156 2573
Fragments arranged by size:
2573 2530 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 3027 3990
Size: 416 53 83 399 24 624 986 963
Cuts at: 3990 4948 442
Size: 958 1753
Fragments arranged by size:
1753 986 963 958 624 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3467
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2306 2657 3088 4919 1239
Size: 977 90 351 431 1831 2579
Fragments arranged by size:
2579 1831 977 431 351 90
Bpu10I CC'TnA_GC
Cuts at: 3135 3135
Size: 6259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4384 5249 5547 4384
Size: 865 298 5096
Fragments arranged by size:
5096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 4922 882
Size: 617 1134 2289 2219
Fragments arranged by size:
2289 2219 1134 617
BsaAI yAC'GTr
Cuts at: 493 1204 2388 4050 493
Size: 711 1184 1662 2702
Fragments arranged by size:
2702 1662 1184 711
BsaBI GATnn'nnATC
Cuts at: 2294 6253 2294
Size: 3959 2300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2223 2436 4066
Size: 53 83 186 936 677 13 213 1630
Cuts at: 4066 4448 5083 275
Size: 382 635 1451
Fragments arranged by size:
1630 1451 936 677 635 382 213 186
83 53 13
BsaWI w'CCGG_w
Cuts at: 3486 4692 5090 5364 5511 5922 3486
Size: 1206 398 274 147 411 3823
Fragments arranged by size:
3823 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 4735
Fragments arranged by size:
4735 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6259
BseYI C'CCAG_C
Cuts at: 3024 5462 5958 3024
Size: 2438 496 3325
Fragments arranged by size:
3325 2438 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3324 1163
Size: 573 1588 4098
Fragments arranged by size:
4098 1588 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4320 4405 5476 4320
Size: 85 1071 5103
Fragments arranged by size:
5103 1071 85
BsmI GAATG_Cn'
Cuts at: 3770 3863 3770
Size: 93 6166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3271 3299 3402 3578 4320 4405 5476
Size: 399 1481 28 103 176 742 85 1071
Cuts at: 5476 1391
Size: 2174
Fragments arranged by size:
2174 1481 1071 742 399 176 103 85
28
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 3429 4148 5878 3429
Size: 719 1730 3810
Fragments arranged by size:
3810 1730 719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2262 3099 3305 3614 844
Size: 1418 837 206 309 3489
Fragments arranged by size:
3489 1418 837 309 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2421 3383 4146 1394
Size: 429 598 962 763 3507
Fragments arranged by size:
3507 962 763 598 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 4754 4928 65
Size: 1183 1284 2222 174 1396
Fragments arranged by size:
2222 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2708 3164 4903 5090 1700
Size: 110 898 456 1739 187 2869
Fragments arranged by size:
2869 1739 898 456 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 2059 1925
Size: 134 6125
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3311 3320 794
Size: 2517 9 3733
Fragments arranged by size:
3733 2517 9
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 3238 2236
Size: 1002 5257
BstYI r'GATC_y
Cuts at: 1972 2726 3589 3956 4341 4358 5810 5896 5908
Size: 754 863 367 385 17 1452 86 12
Cuts at: 5908 6254 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 863 754 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2799 2799
Size: 6259
Bsu36I CC'TnA_GG
Cuts at: 3513 3513
Size: 6259
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 2217 2241 2272 2355 3231
Size: 552 939 108 105 24 31 83 876
Cuts at: 3231 513
Size: 3541
Fragments arranged by size:
3541 939 876 552 108 105 83 31
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3533 487
Size: 683 56 2307 3213
Fragments arranged by size:
3213 2307 683 56
BtsI GCAGTG_nn'
Cuts at: 3330 3775 4569 4589 3330
Size: 445 794 20 5000
Fragments arranged by size:
5000 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2527 2562 529
Size: 35 1963 35 4226
Fragments arranged by size:
4226 1963 35 35
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3515 5266 809
Size: 507 2199 1751 1802
Fragments arranged by size:
2199 1802 1751 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2115 2238
Size: 54 1425 50 275 18 279 6 123
Cuts at: 2238 2269 3706 4595 6224 8
Size: 31 1437 889 1629 43
Fragments arranged by size:
1629 1437 1425 889 279 275 123 54
50 43 31 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2808 3269 4189 1353
Size: 1455 461 920 3423
Fragments arranged by size:
3423 1455 920 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4846 5220 5366 387
Size: 1064 138 3257 374 146 1280
Fragments arranged by size:
3257 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2306 2657 3088 4322 4919 5706
Size: 393 584 90 351 431 1234 597 787
Cuts at: 5706 5974 1239
Size: 268 1524
Fragments arranged by size:
1524 1234 787 597 584 431 393 351
268 90
EcoO109I rG'GnC_Cy
Cuts at: 2147 2154 2301 3021 2147
Size: 7 147 720 5385
Fragments arranged by size:
5385 720 147 7
EcoRI G'AATT_C
Cuts at: 2996 3043 3568 3946 2996
Size: 47 525 378 5309
Fragments arranged by size:
5309 525 378 47
FseI GG_CCGG'CC
Cuts at: 3168 3168
Size: 6259
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 2027 2170 2189 2226 5406 1678
Size: 349 143 19 37 3180 2531
Fragments arranged by size:
3180 2531 349 143 37 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3086 3118 3518 3550 4900
Size: 32 135 32 1514 32 400 32 1350
Cuts at: 4900 4932 4974 5006 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1514 1350 400 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 2530 3603 3849 669
Size: 988 281 394 198 1073 246 3079
Fragments arranged by size:
3079 1073 988 394 281 246 198
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6259
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KasI G'GCGC_C
Cuts at: 2222 2222
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5373 5557 1305
Size: 4068 184 2007
Fragments arranged by size:
4068 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 10
Size: 54 1425 343 408 4029
Fragments arranged by size:
4029 1425 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 3236 4217 4576 4735 518
Size: 648 1530 540 981 359 159 2042
Fragments arranged by size:
2042 1530 981 648 540 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2219 2274 3028 3675 4352 5500 5745
Size: 508 105 55 754 647 677 1148 245
Cuts at: 5745 1606
Size: 2120
Fragments arranged by size:
2120 1148 754 677 647 508 245 105
55
NaeI GCC'GGC
Cuts at: 2710 3166 2710
Size: 456 5803
NarI GG'CG_CC
Cuts at: 2223 2223
Size: 6259
NcoI C'CATG_G
Cuts at: 513 1065 2004 2241 513
Size: 552 939 237 4531
Fragments arranged by size:
4531 939 552 237
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NgoMIV G'CCGG_C
Cuts at: 2708 3164 2708
Size: 456 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3617 5162 1132
Size: 97 2388 1545 2229
Fragments arranged by size:
2388 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6259
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3408 1108
Size: 2300 3959
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2147 2154 2301 3021 2147
Size: 7 147 720 5385
Fragments arranged by size:
5385 720 147 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PstI C_TGCA'G
Cuts at: 830 2259 2882 3325 3611 830
Size: 1429 623 443 286 3478
Fragments arranged by size:
3478 1429 623 443 286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 3028 3028
Size: 6259
SacII CC_GC'GG
Cuts at: 2115 2220 2275 2115
Size: 105 55 6099
Fragments arranged by size:
6099 105 55
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SanDI GG'GwC_CC
Cuts at: 2147 2154 2301 3021 2147
Size: 7 147 720 5385
Fragments arranged by size:
5385 720 147 7
SbfI CC_TGCA'GG
Cuts at: 2259 3611 2259
Size: 1352 4907
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 2255 2318 2878 3321 3607 4742 5423
Size: 219 1210 63 560 443 286 1135 681
Cuts at: 5423 5614 826
Size: 191 1471
Fragments arranged by size:
1471 1210 1135 681 560 443 286 219
191 63
SfoI GGC'GCC
Cuts at: 2224 2224
Size: 6259
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 2021 2145 2152 3586 1872
Size: 149 124 7 1434 4545
Fragments arranged by size:
4545 1434 149 124 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4363 5264 5526 820
Size: 197 926 2420 901 262 1553
Fragments arranged by size:
2420 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SrfI GCCC'GGGC
Cuts at: 2021 2021
Size: 6259
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2424 3056 3698 513
Size: 552 939 237 183 632 642 3074
Fragments arranged by size:
3074 939 642 632 552 237 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2747 4286 4303 4456 4641 5086 325
Size: 1255 1167 1539 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 1167 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 4505 6217
Size: 274 80 33 51 1443 471 2057 1712
Cuts at: 6217 96
Size: 138
Fragments arranged by size:
2057 1712 1443 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 3019 1316
Size: 1173 530 4556
Fragments arranged by size:
4556 1173 530
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3214 2011
Size: 1203 5056
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 2019 2143 2150 3584 1870
Size: 149 124 7 1434 4545
Fragments arranged by size:
4545 1434 149 124 7
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2437 4067 276
Size: 53 83 186 936 903 1630 2468
Fragments arranged by size:
2468 1630 936 903 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SapI SexAI SfiI SwaI