Restriction map for
pFN21AB5688
(Circular) (Six-base) MAPSORT of: pFN21AB5688.seq Check: 7826 from: 1 to: 6790
pFN21AB5688 6790 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3594 4600 278
Size: 53 83 186 936 2058 1006 2468
Fragments arranged by size:
2468 2058 1006 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 4133 1937
Size: 643 1553 4594
Fragments arranged by size:
4594 1553 643
Acc65I G'GTAC_C
Cuts at: 4111 4111
Size: 6790
AclI AA'CG_TT
Cuts at: 1563 4917 5290 1563
Size: 3354 373 3063
Fragments arranged by size:
3354 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2751 3515 3847 4853 6237 6505 1632
Size: 620 499 764 332 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1006 764 620 499 332 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6593
AflIII A'CryG_T
Cuts at: 1203 5689 6666 1203
Size: 4486 977 1327
Fragments arranged by size:
4486 1327 977
AgeI A'CCGG_T
Cuts at: 5621 5621
Size: 6790
AhdI GACnn_n'nnGTC
Cuts at: 5519 5519
Size: 6790
AleI CACnn'nnGTG
Cuts at: 1166 2309 1166
Size: 1143 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 1184
Size: 882 5908
AlwNI CAG_nnn'CTG
Cuts at: 3124 3190 4533 6105 3124
Size: 66 1343 1572 3809
Fragments arranged by size:
3809 1572 1343 66
ApaI G_GGCC'C
Cuts at: 3848 3848
Size: 6790
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 2414 2720 3504 4099 4325 4477 6586 1493
Size: 921 306 784 595 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 784 595 306 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5344 160
Size: 872 4312 1606
Fragments arranged by size:
4312 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 3210 3821 4115 1870
Size: 73 1267 611 294 4545
Fragments arranged by size:
4545 1267 611 294 73
BamHI G'GATC_C
Cuts at: 4120 4487 4120
Size: 367 6423
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2231 2349 2888 3036
Size: 325 217 627 290 154 118 539 148
Cuts at: 3036 3710 3965 4111 5566 618
Size: 674 255 146 1455 1842
Fragments arranged by size:
1842 1455 674 627 539 325 290 255
217 154 148 146 118
BanII G_rGCy'C
Cuts at: 1391 2073 3528 3605 3848 4109 1391
Size: 682 1455 77 243 261 4072
Fragments arranged by size:
4072 1455 682 261 243 77
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 6790
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 2751 928
Size: 971 804 48 4967
Fragments arranged by size:
4967 971 804 48
BbvCI CC'TCA_GC
Cuts at: 3947 3947
Size: 6790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2096 2130 2960 2994 4981 5015 1903
Size: 34 159 34 830 34 1987 34 3678
Fragments arranged by size:
3678 1987 830 159 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2298 4682 5892 2298
Size: 2384 1210 3196
Fragments arranged by size:
3196 2384 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6790
BfrBI ATG'CAT
Cuts at: 2188 2188
Size: 6790
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5401 136
Size: 107 122 71 631 605 337 3392 1525
Fragments arranged by size:
3392 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2737 6785 2737
Size: 4048 2742
BlpI GC'TnA_GC
Cuts at: 2148 4207 2148
Size: 2059 4731
Bme1580I G_kGCm'C
Cuts at: 1790 3848 4851 6007 1790
Size: 2058 1003 1156 2573
Fragments arranged by size:
2573 2058 1156 1003
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2214 4521 5479
Size: 416 53 83 399 24 797 2307 958
Cuts at: 5479 442
Size: 1753
Fragments arranged by size:
2307 1753 958 797 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3138 3138
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3047 3272 3290 3699 4094 5450 1239
Size: 1808 225 18 409 395 1356 2579
Fragments arranged by size:
2579 1808 1356 409 395 225 18
Bpu10I CC'TnA_GC
Cuts at: 3015 3947 3015
Size: 932 5858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4915 5780 6078 4915
Size: 865 298 5627
Fragments arranged by size:
5627 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2966 3191 3572 5453 882
Size: 617 1467 225 381 1881 2219
Fragments arranged by size:
2219 1881 1467 617 381 225
BsaAI yAC'GTr
Cuts at: 493 1204 3439 4581 493
Size: 711 2235 1142 2702
Fragments arranged by size:
2702 2235 1142 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 3591 4597 4979
Size: 53 83 186 936 545 1513 1006 382
Cuts at: 4979 5614 275
Size: 635 1451
Fragments arranged by size:
1513 1451 1006 936 635 545 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5223 5621 5895 6042 6453 5223
Size: 398 274 147 411 5560
Fragments arranged by size:
5560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6565
Fragments arranged by size:
6565 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2873 2928 3623 3632 1186
Size: 1687 55 695 9 4344
Fragments arranged by size:
4344 1687 695 55 9
BseYI C'CCAG_C
Cuts at: 3652 5993 6489 3652
Size: 2341 496 3953
Fragments arranged by size:
3953 2341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3564 1163
Size: 573 1828 4389
Fragments arranged by size:
4389 1828 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 4240 5001 6029 1540
Size: 275 186 27 2212 761 1028 2301
Fragments arranged by size:
2301 2212 1028 761 275 186 27
BsiHKAI G_wGCw'C
Cuts at: 3528 3605 3883 4851 4936 6007 3528
Size: 77 278 968 85 1071 4311
Fragments arranged by size:
4311 1071 968 278 85 77
BsmI GAATG_Cn'
Cuts at: 3300 4301 4394 3300
Size: 1001 93 5696
Fragments arranged by size:
5696 1001 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 3528 3605 3848 3883 4109 4851
Size: 399 283 1455 77 243 35 226 742
Cuts at: 4851 4936 6007 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1455 1071 742 399 283 243 226
85 77 35
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 2914 4679 6409 2914
Size: 1765 1730 3295
Fragments arranged by size:
3295 1765 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4145 844
Size: 3301 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3832 4677 1394
Size: 429 647 1362 845 3507
Fragments arranged by size:
3507 1362 845 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 2375 3912 5285 5459 65
Size: 1183 921 206 1537 1373 174 1396
Fragments arranged by size:
1537 1396 1373 1183 921 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 5434 5621 1700
Size: 110 286 3338 187 2869
Fragments arranged by size:
3338 2869 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6790
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6790
BssSI C'ACGA_G
Cuts at: 2405 4850 5862 2405
Size: 2445 1012 3333
Fragments arranged by size:
3333 2445 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2123 794
Size: 1329 5461
BstBI TT'CG_AA
Cuts at: 5594 5594
Size: 6790
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6790
BstXI CCAn_nnnn'nTGG
Cuts at: 2591 2591
Size: 6790
BstYI r'GATC_y
Cuts at: 1972 2633 2737 3621 3674 4120 4487 4872 4889
Size: 661 104 884 53 446 367 385 17
Cuts at: 4889 6341 6427 6439 6785 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 884 661 446 385 367 346
104 86 53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 2037 2590 3144 3287 3840
Size: 552 939 18 15 553 554 143 553
Cuts at: 3840 3918 3976 4042 513
Size: 78 58 66 3261
Fragments arranged by size:
3261 939 554 553 553 552 143 78
66 58 18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3281 3886 487
Size: 683 56 2055 605 3391
Fragments arranged by size:
3391 2055 683 605 56
BtsI GCAGTG_nn'
Cuts at: 3561 3848 3883 3912 4306 5100 5120 3561
Size: 287 35 29 394 794 20 5231
Fragments arranged by size:
5231 794 394 287 35 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6755
DraI TTT'AAA
Cuts at: 4094 4094
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2025 2094
Size: 54 1425 50 275 18 180 15 69
Cuts at: 2094 3909 4237 5126 6755 8
Size: 1815 328 889 1629 43
Fragments arranged by size:
1815 1629 1425 889 328 275 180 69
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2025 4237 1537
Size: 275 213 2212 4090
Fragments arranged by size:
4090 2212 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 2649 2661 2667 2673 2685 3069 3711
Size: 1290 6 12 6 6 12 384 642
Cuts at: 3711 3822 3947 4008 4720 1353
Size: 111 125 61 712 3423
Fragments arranged by size:
3423 1290 712 642 384 125 111 61
12 12 6 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5377 5751 5897 387
Size: 1064 138 3788 374 146 1280
Fragments arranged by size:
3788 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3526 3603 3526
Size: 77 6713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2751 3047 3272 3290 3515 3699
Size: 393 620 499 296 225 18 225 184
Cuts at: 3699 3847 4094 4853 5450 6237 6505 1239
Size: 148 247 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 620 597 499 393 296
268 247 225 225 184 148 18
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2282 2398 2260
Size: 22 116 6652
Fragments arranged by size:
6652 116 22
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 2081 2093 5937 1678
Size: 403 12 3844 2531
Fragments arranged by size:
3844 2531 403 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3415 3447 3821 3853 5431
Size: 32 135 32 1843 32 374 32 1578
Cuts at: 5431 5463 5505 5537 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1843 1578 374 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3388 4134 4380 669
Size: 988 281 1450 746 246 3079
Fragments arranged by size:
3079 1450 988 746 281 246
HindIII A'AGCT_T
Cuts at: 748 3153 748
Size: 2405 4385
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 6790
KpnI G_GTAC'C
Cuts at: 4115 4115
Size: 6790
MfeI C'AATT_G
Cuts at: 4389 4389
Size: 6790
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2249 2957 3221 3793 5904 6088 1305
Size: 944 708 264 572 2111 184 2007
Fragments arranged by size:
2111 2007 944 708 572 264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3911 10
Size: 54 1425 343 2079 2889
Fragments arranged by size:
2889 2079 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3905 4047 4748 5107 5266 518
Size: 648 1143 1596 142 701 359 159 2042
Fragments arranged by size:
2042 1596 1143 701 648 359 159 142
MspA1I CmG'CkG
Cuts at: 1606 2024 2329 2762 3025 3190 4206 4883 6031
Size: 418 305 433 263 165 1016 677 1148
Cuts at: 6031 6276 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1016 677 433 418 305 263
245 165
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 6790
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 6790
NcoI C'CATG_G
Cuts at: 513 1065 2004 2590 3144 4042 513
Size: 552 939 586 554 898 3261
Fragments arranged by size:
3261 939 898 586 554 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6790
NotI GC'GGCC_GC
Cuts at: 2025 4237 2025
Size: 2212 4578
NsiI A_TGCA'T
Cuts at: 2190 2190
Size: 6790
NspI r_CATG'y
Cuts at: 1132 1229 2192 2526 2563 2999 3377 4148 5693
Size: 97 963 334 37 436 378 771 1545
Cuts at: 5693 1132
Size: 2229
Fragments arranged by size:
2229 1545 963 771 436 378 334 97
37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6790
PciI A'CATG_T
Cuts at: 5689 5689
Size: 6790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3145 1108
Size: 2037 4753
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PmlI CAC'GTG
Cuts at: 3439 3439
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 6372 6404 4859
Size: 32 1481 32 5245
Fragments arranged by size:
5245 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3237 3864 1613
Size: 1624 627 4539
Fragments arranged by size:
4539 1624 627
PsiI TTA'TAA
Cuts at: 2712 4360 2712
Size: 1648 5142
PspOMI G'GGCC_C
Cuts at: 3844 3844
Size: 6790
PspXI vC'TCGA_Gb
Cuts at: 3821 3821
Size: 6790
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4046 4078 4046
Size: 32 6758
PstI C_TGCA'G
Cuts at: 830 2983 4142 830
Size: 2153 1159 3478
Fragments arranged by size:
3478 2153 1159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 2762 3025 3190 2762
Size: 263 165 6362
Fragments arranged by size:
6362 263 165
SacI G_AGCT'C
Cuts at: 3528 3605 3528
Size: 77 6713
SacII CC_GC'GG
Cuts at: 2025 2025
Size: 6790
SalI G'TCGA_C
Cuts at: 1936 4132 1936
Size: 2196 4594
SapI GCTCTTCn'nnn_
Cuts at: 3822 4008 3822
Size: 186 6604
SbfI CC_TGCA'GG
Cuts at: 4142 4142
Size: 6790
ScaI AGT'ACT
Cuts at: 2273 5038 6750 2273
Size: 2765 1712 2313
Fragments arranged by size:
2765 2313 1712
SfcI C'TryA_G
Cuts at: 826 1045 2297 2979 4138 5273 5954 6145 826
Size: 219 1252 682 1159 1135 681 191 1471
Fragments arranged by size:
1471 1252 1159 1135 682 681 219 191
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 6790
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 4117 1872
Size: 2245 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3821 4894 5795 6057 820
Size: 197 926 1878 1073 901 262 1553
Fragments arranged by size:
1878 1553 1073 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6790
SphI G_CATG'C
Cuts at: 1132 2563 2999 4148 1132
Size: 1431 436 1149 3774
Fragments arranged by size:
3774 1431 1149 436
SspI AAT'ATT
Cuts at: 5 52 4714 5
Size: 47 4662 2081
Fragments arranged by size:
4662 2081 47
StuI AGG'CCT
Cuts at: 1517 3311 3819 1517
Size: 1794 508 4488
Fragments arranged by size:
4488 1794 508
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2590 3144 4042 4229 513
Size: 552 939 81 505 554 898 187 3074
Fragments arranged by size:
3074 939 898 554 552 505 187 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2399 2829 4817 4834 4987 5172 5617
Size: 1255 819 430 1988 17 153 185 445
Cuts at: 5617 325
Size: 1498
Fragments arranged by size:
1988 1498 1255 819 445 430 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 5036 6748
Size: 274 80 33 51 1443 294 2765 1712
Cuts at: 6748 96
Size: 138
Fragments arranged by size:
2765 1712 1443 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3666 3698 6750 6782 3666
Size: 32 3052 32 3674
Fragments arranged by size:
3674 3052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 4126 4126
Size: 6790
XhoI C'TCGA_G
Cuts at: 1943 3821 1943
Size: 1878 4912
XmaI C'CCGG_G
Cuts at: 1870 4115 1870
Size: 2245 4545
XmnI GAAnn'nnTTC
Cuts at: 2262 2265 4919 2262
Size: 3 2654 4133
Fragments arranged by size:
4133 2654 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3592 4598 276
Size: 53 83 186 936 2058 1006 2468
Fragments arranged by size:
2468 2058 1006 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI
NruI PacI PfoI PpuMI RsrII SanDI SexAI SfiI
SrfI SwaI XcmI