Restriction map for
pFN21AB5685
(Circular) (Six-base) MAPSORT of: pFN21AB5685.seq Check: 8955 from: 1 to: 5911
pFN21AB5685 5911 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2036 2128 2036
Size: 92 5819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2925 3254 1937
Size: 988 329 4594
Fragments arranged by size:
4594 988 329
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2398 2402 2940 3974 5358 5626 1632
Size: 766 4 538 1034 1384 268 1917
Fragments arranged by size:
1917 1384 1034 766 538 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 2964 4810 5787 1203
Size: 1761 1846 977 1327
Fragments arranged by size:
1846 1761 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 2856 3034 3654 5226 2856
Size: 178 620 1572 3541
Fragments arranged by size:
3541 1572 620 178
ApaLI G'TGCA_C
Cuts at: 2075 3968 5124 2075
Size: 1893 1156 2862
Fragments arranged by size:
2862 1893 1156
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 3236 1870
Size: 73 1176 117 4545
Fragments arranged by size:
4545 1176 117 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2806 3232 4687 618
Size: 325 217 627 1019 426 1455 1842
Fragments arranged by size:
1842 1455 1019 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 2319 3230 1391
Size: 928 911 4072
Fragments arranged by size:
4072 928 911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 2606 928
Size: 971 602 105 4233
Fragments arranged by size:
4233 971 602 105
BbvCI CC'TCA_GC
Cuts at: 2771 2771
Size: 5911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4102 4136 1903
Size: 34 50 34 2081 34 3678
Fragments arranged by size:
3678 2081 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 2514 1548
Size: 966 4945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 3113 4522
Size: 107 122 71 631 605 433 1008 1409
Cuts at: 4522 136
Size: 1525
Fragments arranged by size:
1525 1409 1008 631 605 433 122 107
71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2673 2842 3972 5128 1790
Size: 289 594 169 1130 1156 2573
Fragments arranged by size:
2573 1156 1130 594 289 169
BmgBI CAC'GTC
Cuts at: 1318 3022 1318
Size: 1704 4207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 3642 4600
Size: 416 53 83 399 24 1080 1145 958
Cuts at: 4600 442
Size: 1753
Fragments arranged by size:
1753 1145 1080 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2001 2195 2602 2933 4571 1239
Size: 762 194 407 331 1638 2579
Fragments arranged by size:
2579 1638 762 407 331 194
Bpu10I CC'TnA_GC
Cuts at: 2771 2771
Size: 5911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2095 3702 493
Size: 711 891 1607 2702
Fragments arranged by size:
2702 1607 891 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3718 4100 4735 275
Size: 53 83 186 936 2185 382 635 1451
Fragments arranged by size:
2185 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2424 4344 4742 5016 5163 5574 2424
Size: 1920 398 274 147 411 2761
Fragments arranged by size:
2761 1920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2241 3070 3137 1186
Size: 1055 829 67 3960
Fragments arranged by size:
3960 1055 829 67
BseYI C'CCAG_C
Cuts at: 2235 2841 5114 5610 2235
Size: 606 2273 496 2536
Fragments arranged by size:
2536 2273 606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2319 2432 3972 4057 5128 2079
Size: 240 113 1540 85 1071 2862
Fragments arranged by size:
2862 1540 1071 240 113 85
BsmI GAATG_Cn'
Cuts at: 2578 3422 3515 2578
Size: 844 93 4974
Fragments arranged by size:
4974 844 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2319 2432 2673 2842 3230 3972
Size: 399 289 240 113 241 169 388 742
Cuts at: 3972 4057 5128 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 289 241 240
169 113 85
BspEI T'CCGG_A
Cuts at: 2424 5574 2424
Size: 3150 2761
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2036 2128 3266 844
Size: 1192 92 1138 3489
Fragments arranged by size:
3489 1192 1138 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2781 4406 4580 65
Size: 1183 1533 1625 174 1396
Fragments arranged by size:
1625 1533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 2307 96
Size: 2211 3700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 2992 3971 4983 2992
Size: 979 1012 3920
Fragments arranged by size:
3920 1012 979
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 794
Size: 2240 3671
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2377 3241 3608 3993 4010 5462 5548 5560
Size: 405 864 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 864 405 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2930 2930
Size: 5911
BtgI C'CryG_G
Cuts at: 513 1065 2327 2541 2758 513
Size: 552 1262 214 217 3666
Fragments arranged by size:
3666 1262 552 217 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 3427 4221 4241 3427
Size: 794 20 5097
Fragments arranged by size:
5097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2590 2725
Size: 54 1425 50 275 18 372 388 135
Cuts at: 2725 3358 4247 5876 8
Size: 633 889 1629 43
Fragments arranged by size:
1629 1425 889 633 388 372 275 135
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 3841 1353
Size: 1289 1199 3423
Fragments arranged by size:
3423 1289 1199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2317
Size: 5911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2195 2398 2402 2602 2933 2940
Size: 393 369 194 203 4 200 331 7
Cuts at: 2940 3974 4571 5358 5626 1239
Size: 1034 597 787 268 1524
Fragments arranged by size:
1524 1034 787 597 393 369 331 268
203 200 194 7 4
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 5911
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 3035 5058 1678
Size: 1357 2023 2531
Fragments arranged by size:
2531 2023 1357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2463 2495 3132 3164 4552
Size: 32 135 32 891 32 637 32 1388
Cuts at: 4552 4584 4626 4658 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1388 891 637 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 3255 3501 669
Size: 988 281 988 329 246 3079
Fragments arranged by size:
3079 988 988 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5025 5209 1305
Size: 3720 184 2007
Fragments arranged by size:
3720 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 2727 10
Size: 54 1425 343 372 523 3194
Fragments arranged by size:
3194 1425 523 372 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3869 4228 4387 518
Size: 648 843 1860 359 159 2042
Fragments arranged by size:
2042 1860 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2374 2551 3092 3327 4004 5152 5397
Size: 609 159 177 541 235 677 1148 245
Cuts at: 5397 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 609 541 245 235 177
159
NcoI C'CATG_G
Cuts at: 513 1065 2327 513
Size: 552 1262 4097
Fragments arranged by size:
4097 1262 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 2779 2968 3269 4814 1132
Size: 97 1550 189 301 1545 2229
Fragments arranged by size:
2229 1550 1545 301 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 2964 4810 2964
Size: 1846 4065
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3202 1108
Size: 2094 3817
PfoI T'CCnGG_A
Cuts at: 2742 2742
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2921 1613
Size: 1308 4603
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PstI C_TGCA'G
Cuts at: 830 3039 3263 830
Size: 2209 224 3478
Fragments arranged by size:
3478 2209 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2215 2551 3092 2215
Size: 336 541 5034
Fragments arranged by size:
5034 541 336
SacI G_AGCT'C
Cuts at: 2319 2319
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 2924 3253 1936
Size: 988 329 4594
Fragments arranged by size:
4594 988 329
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 3035 3259 4394 5075 5266 826
Size: 219 1990 224 1135 681 191 1471
Fragments arranged by size:
1990 1471 1135 681 224 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2105 2105
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 2150 1517
Size: 633 5278
StyI C'CwwG_G
Cuts at: 513 1065 2154 2327 3350 513
Size: 552 1089 173 1023 3074
Fragments arranged by size:
3074 1089 1023 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2479 2639 2975 3938 3955 4108 4293
Size: 1255 899 160 336 963 17 153 185
Cuts at: 4293 4738 325
Size: 445 1498
Fragments arranged by size:
1498 1255 963 899 445 336 185 160
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 4157 5869
Size: 274 80 33 51 1443 330 1850 1712
Cuts at: 5869 96
Size: 138
Fragments arranged by size:
1850 1712 1443 330 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2908 2908
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfoI SrfI SwaI