Restriction map for
pFN21AB5682
(Circular) (Six-base) MAPSORT of: pFN21AB5682.seq Check: 3488 from: 1 to: 6361
pFN21AB5682 6361 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2686 3704 1937
Size: 749 1018 4594
Fragments arranged by size:
4594 1018 749
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 2234 2547 2852 4424 5808 6076 1632
Size: 533 69 313 305 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 533 313 305 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 2335 5260 6237 1203
Size: 1132 2925 977 1327
Fragments arranged by size:
2925 1327 1132 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 2014 5090 2014
Size: 3076 3285
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2472 3179 1184
Size: 1288 707 4366
Fragments arranged by size:
4366 1288 707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3264 3296 3264
Size: 32 6329
AlwNI CAG_nnn'CTG
Cuts at: 3380 3464 4104 5676 3380
Size: 84 640 1572 4065
Fragments arranged by size:
4065 1572 640 84
ApaI G_GGCC'C
Cuts at: 3601 3601
Size: 6361
ApaLI G'TGCA_C
Cuts at: 3264 4418 5574 3264
Size: 1154 1156 4051
Fragments arranged by size:
4051 1156 1154
ApoI r'AATT_y
Cuts at: 1493 3582 3670 3896 4048 6157 1493
Size: 2089 88 226 152 2109 1697
Fragments arranged by size:
2109 2089 1697 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 3686 1870
Size: 73 1743 4545
Fragments arranged by size:
4545 1743 73
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3204 3682 5137 618
Size: 325 217 627 1417 478 1455 1842
Fragments arranged by size:
1842 1455 1417 627 478 325 217
BanII G_rGCy'C
Cuts at: 1391 2203 3601 3680 1391
Size: 812 1398 79 4072
Fragments arranged by size:
4072 1398 812 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5390
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 6361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4253 5463 3053
Size: 1200 1210 3951
Fragments arranged by size:
3951 1210 1200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BfrBI ATG'CAT
Cuts at: 3501 3501
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4972 136
Size: 107 122 71 631 605 3300 1525
Fragments arranged by size:
3300 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3288 6356 3288
Size: 3068 3293
BlpI GC'TnA_GC
Cuts at: 3778 3778
Size: 6361
Bme1580I G_kGCm'C
Cuts at: 1790 3268 3601 4422 5578 1790
Size: 1478 333 821 1156 2573
Fragments arranged by size:
2573 1478 1156 821 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2699 4092 5050
Size: 416 53 83 399 24 1282 1393 958
Cuts at: 5050 442
Size: 1753
Fragments arranged by size:
1753 1393 1282 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2475 2475
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2320 3096 3291 3426 3587 5021 1239
Size: 1081 776 195 135 161 1434 2579
Fragments arranged by size:
2579 1434 1081 776 195 161 135
Bpu10I CC'TnA_GC
Cuts at: 2736 2736
Size: 6361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5024 882
Size: 617 3525 2219
Fragments arranged by size:
3525 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3064 4152 493
Size: 711 1860 1088 2702
Fragments arranged by size:
2702 1860 1088 711
BsaBI GATnn'nnATC
Cuts at: 6355 6355
Size: 6361
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4168 4550 5185 275
Size: 53 83 186 936 2635 382 635 1451
Fragments arranged by size:
2635 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3535 4794 5192 5466 5613 6024 3535
Size: 1259 398 274 147 411 3872
Fragments arranged by size:
3872 1259 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3264 3294 1178
Size: 30 165 30 1861 30 4245
Fragments arranged by size:
4245 1861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3093 1186
Size: 1907 4454
BseYI C'CCAG_C
Cuts at: 2596 3257 3528 5564 6060 2596
Size: 661 271 2036 496 2897
Fragments arranged by size:
2897 2036 661 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2808 3185 3348 1163
Size: 573 1072 377 163 4176
Fragments arranged by size:
4176 1072 573 377 163
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 3268 4422 4507 5578 2203
Size: 1065 1154 85 1071 2986
Fragments arranged by size:
2986 1154 1071 1065 85
BsmI GAATG_Cn'
Cuts at: 2930 3872 3965 2930
Size: 942 93 5326
Fragments arranged by size:
5326 942 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2300 2300
Size: 6361
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 3268 3601 3680 4422 4507 5578
Size: 399 413 1065 333 79 742 85 1071
Cuts at: 5578 1391
Size: 2174
Fragments arranged by size:
2174 1071 1065 742 413 399 333 85
79
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3716 844
Size: 2872 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2979 4856 5030 65
Size: 1183 1731 1877 174 1396
Fragments arranged by size:
1877 1731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3229 96
Size: 3133 3228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3371 3380 794
Size: 2577 9 3775
Fragments arranged by size:
3775 2577 9
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6361
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2689
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 3288 3691 4058 4443 4460 5912 5998 6010
Size: 1316 403 367 385 17 1452 86 12
Cuts at: 6010 6356 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1316 403 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2687 2687
Size: 6361
BtgI C'CryG_G
Cuts at: 513 1065 2004 3396 513
Size: 552 939 1392 3478
Fragments arranged by size:
3478 1392 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 2634 3877 4671 4691 2444
Size: 190 1243 794 20 4114
Fragments arranged by size:
4114 1243 794 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3221 5368 809
Size: 507 1905 2147 1802
Fragments arranged by size:
2147 1905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3808 4697
Size: 54 1425 50 275 18 1030 948 889
Cuts at: 4697 6326 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1030 948 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4291 1353
Size: 2938 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4948 5322 5468 387
Size: 1064 138 3359 374 146 1280
Fragments arranged by size:
3359 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2201 2201
Size: 6361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2234 2320 2547 2852 3096 3291
Size: 393 533 69 86 227 305 244 195
Cuts at: 3291 3426 3587 4424 5021 5808 6076 1239
Size: 135 161 837 597 787 268 1524
Fragments arranged by size:
1524 837 787 597 533 393 305 268
244 227 195 161 135 86 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2739 3075 3417 2739
Size: 336 342 5683
Fragments arranged by size:
5683 342 336
EcoO109I rG'GnC_Cy
Cuts at: 2732 3598 2732
Size: 866 5495
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FspI TGC'GCA
Cuts at: 4867 4867
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 5508 1678
Size: 3830 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 3540 3572 5002
Size: 32 135 32 428 32 1508 32 1430
Cuts at: 5002 5034 5076 5108 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 1430 428 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3101 3705 3951 669
Size: 988 281 1163 604 246 3079
Fragments arranged by size:
3079 1163 988 604 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 2121 3960 2121
Size: 1839 4522
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2243 3150 5475 5659 1305
Size: 938 907 2325 184 2007
Fragments arranged by size:
2325 2007 938 907 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4539
Fragments arranged by size:
4539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2322 2657 4319 4678 4837 518
Size: 648 1156 335 1662 359 159 2042
Fragments arranged by size:
2042 1662 1156 648 359 335 159
MspA1I CmG'CkG
Cuts at: 1606 3380 3461 3777 4454 5602 5847 1606
Size: 1774 81 316 677 1148 245 2120
Fragments arranged by size:
2120 1774 1148 677 316 245 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4870
Fragments arranged by size:
4870 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NsiI A_TGCA'T
Cuts at: 3503 3503
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 2339 3719 5264 1132
Size: 97 1110 1380 1545 2229
Fragments arranged by size:
2229 1545 1380 1110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 2335 5260 2335
Size: 2925 3436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6361
PfoI T'CCnGG_A
Cuts at: 2896 2896
Size: 6361
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3264 3296 4430 4462 5943 5975 3264
Size: 32 1134 32 1481 32 3650
Fragments arranged by size:
3650 1481 1134 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3489 1613
Size: 1876 4485
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PspOMI G'GGCC_C
Cuts at: 3597 3597
Size: 6361
PstI C_TGCA'G
Cuts at: 830 2451 3376 3713 830
Size: 1621 925 337 3478
Fragments arranged by size:
3478 1621 925 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 3380 3461 3380
Size: 81 6280
SacI G_AGCT'C
Cuts at: 2203 2203
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 3621 4609 6321 3621
Size: 988 1712 3661
Fragments arranged by size:
3661 1712 988
SfcI C'TryA_G
Cuts at: 826 1045 2447 3372 3709 4844 5525 5716 826
Size: 219 1402 925 337 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 925 681 337 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3688 1872
Size: 1816 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4465 5366 5628 820
Size: 197 926 2522 901 262 1553
Fragments arranged by size:
2522 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SspI AAT'ATT
Cuts at: 5 52 2345 4285 5
Size: 47 2293 1940 2081
Fragments arranged by size:
2293 2081 1940 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6361
StyI C'CwwG_G
Cuts at: 513 1065 2004 3800 513
Size: 552 939 1796 3074
Fragments arranged by size:
3074 1796 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4388 4405 4558 4743 5188 325
Size: 1255 2808 17 153 185 445 1498
Fragments arranged by size:
2808 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3229 3619 4607
Size: 274 80 33 51 1443 1252 390 988
Cuts at: 4607 6319 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1252 988 390 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6321 6353 6321
Size: 32 6329
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2087 2656 2087
Size: 569 5792
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6361
XmaI C'CCGG_G
Cuts at: 1870 3686 1870
Size: 1816 4545
XmnI GAAnn'nnTTC
Cuts at: 3621 4490 3621
Size: 869 5492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BsiWI Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI