Restriction map for
pFN21AB5679
(Circular) (Six-base) MAPSORT of: pFN21AB5679.seq Check: 5798 from: 1 to: 7585
pFN21AB5679 7585 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3047 4600 5395 278
Size: 53 83 186 936 1511 1553 795 2468
Fragments arranged by size:
2468 1553 1511 936 795 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4928 1937
Size: 2991 4594
Acc65I G'GTAC_C
Cuts at: 3753 4906 3753
Size: 1153 6432
AclI AA'CG_TT
Cuts at: 1563 5712 6085 1563
Size: 4149 373 3063
Fragments arranged by size:
4149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 2936 4169 4538 5648 7032 7300 1632
Size: 722 582 1233 369 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1233 1110 722 582 369 268
AfeI AGC'GCT
Cuts at: 3698 3698
Size: 7585
AflII C'TTAA_G
Cuts at: 820 1017 3176 820
Size: 197 2159 5229
Fragments arranged by size:
5229 2159 197
AflIII A'CryG_T
Cuts at: 1203 6484 7461 1203
Size: 5281 977 1327
Fragments arranged by size:
5281 1327 977
AgeI A'CCGG_T
Cuts at: 6416 6416
Size: 7585
AhdI GACnn_n'nnGTC
Cuts at: 6314 6314
Size: 7585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7585
AlwNI CAG_nnn'CTG
Cuts at: 5328 6900 5328
Size: 1572 6013
ApaI G_GGCC'C
Cuts at: 3742 3742
Size: 7585
ApaLI G'TGCA_C
Cuts at: 5642 6798 5642
Size: 1156 6429
ApoI r'AATT_y
Cuts at: 1493 2580 3295 3422 3869 4473 4661 4894 5120
Size: 1087 715 127 447 604 188 233 226
Cuts at: 5120 5272 7381 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1087 715 604 447 233 226
188 152 127
AseI AT'TA_AT
Cuts at: 160 1032 6139 160
Size: 872 5107 1606
Fragments arranged by size:
5107 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
AvaI C'yCGr_G
Cuts at: 1870 1943 4910 1870
Size: 73 2967 4545
Fragments arranged by size:
4545 2967 73
BamHI G'GATC_C
Cuts at: 2149 3553 4915 5282 2149
Size: 1404 1362 367 4452
Fragments arranged by size:
4452 1404 1362 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3753 4906 6361 618
Size: 325 217 627 1966 1153 1455 1842
Fragments arranged by size:
1966 1842 1455 1153 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3742 4235 4676 4904 1391
Size: 2351 493 441 228 4072
Fragments arranged by size:
4072 2351 493 441 228
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3729 928
Size: 971 1830 4784
Fragments arranged by size:
4784 1830 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5776 5810 1903
Size: 34 3839 34 3678
Fragments arranged by size:
3839 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5477 6687 5477
Size: 1210 6375
BclI T'GATC_A
Cuts at: 1548 4692 1548
Size: 3144 4441
BfrBI ATG'CAT
Cuts at: 2103 2867 2103
Size: 764 6821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6196 136
Size: 107 122 71 631 605 4524 1525
Fragments arranged by size:
4524 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2344 7580 2344
Size: 5236 2349
BlpI GC'TnA_GC
Cuts at: 5002 5002
Size: 7585
Bme1580I G_kGCm'C
Cuts at: 1790 3742 5646 6802 1790
Size: 1952 1904 1156 2573
Fragments arranged by size:
2573 1952 1904 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2159 2208 2314
Size: 416 53 83 399 24 742 49 106
Cuts at: 2314 4324 5316 6274 442
Size: 2010 992 958 1753
Fragments arranged by size:
2010 1753 992 958 742 416 399 106
83 53 49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3024 3617 3689 4240 4859 6245 1239
Size: 1785 593 72 551 619 1386 2579
Fragments arranged by size:
2579 1785 1386 619 593 551 72
Bpu10I CC'TnA_GC
Cuts at: 2204 2204
Size: 7585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 4154 4758 4787 5710 6575 6873 3383
Size: 771 604 29 923 865 298 4095
Fragments arranged by size:
4095 923 865 771 604 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6248 882
Size: 617 4749 2219
Fragments arranged by size:
4749 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5376 493
Size: 711 4172 2702
Fragments arranged by size:
4172 2702 711
BsaBI GATnn'nnATC
Cuts at: 4694 7579 4694
Size: 2885 4700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3044 4597 5392 5774
Size: 53 83 186 936 1511 1553 795 382
Cuts at: 5774 6409 275
Size: 635 1451
Fragments arranged by size:
1553 1511 1451 936 795 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6018 6416 6690 6837 7248 6018
Size: 398 274 147 411 6355
Fragments arranged by size:
6355 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7360
Fragments arranged by size:
7360 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3431 1186
Size: 2245 5340
BseYI C'CCAG_C
Cuts at: 6788 7284 6788
Size: 496 7089
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4038 1163
Size: 573 2302 4710
Fragments arranged by size:
4710 2302 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5035 5796 6824 1540
Size: 275 186 3034 761 1028 2301
Fragments arranged by size:
3034 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4402 5646 5731 6802 4402
Size: 1244 85 1071 5185
Fragments arranged by size:
5185 1244 1071 85
BsmI GAATG_Cn'
Cuts at: 2365 5096 5189 2365
Size: 2731 93 4761
Fragments arranged by size:
4761 2731 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3035 3035
Size: 7585
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3742 4235 4402 4676 4904 5646 5731
Size: 399 1952 493 167 274 228 742 85
Cuts at: 5731 6802 1391
Size: 1071 2174
Fragments arranged by size:
2174 1952 1071 742 493 399 274 228
167 85
BspEI T'CCGG_A
Cuts at: 7248 7248
Size: 7585
BspHI T'CATG_A
Cuts at: 2437 3994 5474 7204 2437
Size: 1557 1480 1730 2818
Fragments arranged by size:
2818 1730 1557 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4940 844
Size: 4096 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4257 5472 1394
Size: 429 2434 1215 3507
Fragments arranged by size:
3507 2434 1215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 2317 6080 6254 65
Size: 1183 780 289 3763 174 1396
Fragments arranged by size:
3763 1396 1183 780 289 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6229 6416 1700
Size: 110 4419 187 2869
Fragments arranged by size:
4419 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7585
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7585
BssSI C'ACGA_G
Cuts at: 3784 5645 6657 3784
Size: 1861 1012 4712
Fragments arranged by size:
4712 1861 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7585
BstBI TT'CG_AA
Cuts at: 6389 6389
Size: 7585
BstEII G'GTnAC_C
Cuts at: 1184 3094 1184
Size: 1910 5675
BstXI CCAn_nnnn'nTGG
Cuts at: 3134 3134
Size: 7585
BstYI r'GATC_y
Cuts at: 1972 2149 2344 3553 3841 4915 5282 5667 5684
Size: 177 195 1209 288 1074 367 385 17
Cuts at: 5684 7136 7222 7234 7580 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1209 1074 385 367 346 288
195 177 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3972 3972
Size: 7585
BtgI C'CryG_G
Cuts at: 513 1065 3009 513
Size: 552 1944 5089
Fragments arranged by size:
5089 1944 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6846
Fragments arranged by size:
6846 683 56
BtsI GCAGTG_nn'
Cuts at: 2450 3047 5101 5895 5915 2450
Size: 597 2054 794 20 4120
Fragments arranged by size:
4120 2054 794 597 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7550
DraI TTT'AAA
Cuts at: 4377 4889 4377
Size: 512 7073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2246 6592 809
Size: 507 930 4346 1802
Fragments arranged by size:
4346 1802 930 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5032 5921 7550
Size: 54 1425 50 275 18 3202 889 1629
Cuts at: 7550 8
Size: 43
Fragments arranged by size:
3202 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5032 1537
Size: 275 3220 4090
Fragments arranged by size:
4090 3220 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2674 3076 4328 5515 1353
Size: 1321 402 1252 1187 3423
Fragments arranged by size:
3423 1321 1252 1187 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6172 6546 6692 387
Size: 1064 138 4583 374 146 1280
Fragments arranged by size:
4583 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2936 3024 3617 3689 4169 4240
Size: 393 722 582 88 593 72 480 71
Cuts at: 4240 4538 4859 5648 6245 7032 7300 1239
Size: 298 321 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 722 597 593 582 480
393 321 298 268 88 72 71
EcoO109I rG'GnC_Cy
Cuts at: 3022 3738 3739 3022
Size: 716 1 6868
Fragments arranged by size:
6868 716 1
EcoRI G'AATT_C
Cuts at: 2580 3295 4894 5272 2580
Size: 715 1599 378 4893
Fragments arranged by size:
4893 1599 715 378
EcoRV GAT'ATC
Cuts at: 2950 4156 2950
Size: 1206 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2762 2762
Size: 7585
FspI TGC'GCA
Cuts at: 6091 6091
Size: 7585
HaeII r_GCGC'y
Cuts at: 1678 3700 6732 1678
Size: 2022 3032 2531
Fragments arranged by size:
3032 2531 2022
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2804 2836 3515 3547 3820
Size: 32 135 32 1232 32 679 32 273
Cuts at: 3820 3852 4217 4249 4486 4518 4658 4690 6226
Size: 32 365 32 237 32 140 32 1536
Cuts at: 6226 6258 6300 6332 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1536 1232 679 365 273 237 140
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4491 4929 5175 669
Size: 988 281 2553 438 246 3079
Fragments arranged by size:
3079 2553 988 438 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7585
HpaI GTT'AAC
Cuts at: 5175 5175
Size: 7585
KpnI G_GTAC'C
Cuts at: 3757 4910 3757
Size: 1153 6432
MfeI C'AATT_G
Cuts at: 2727 5184 2727
Size: 2457 5128
MluI A'CGCG_T
Cuts at: 7461 7461
Size: 7585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 3706 4090 4877 6699 6883 1305
Size: 1425 976 384 787 1822 184 2007
Fragments arranged by size:
2007 1822 1425 976 787 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5763
Fragments arranged by size:
5763 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4688 5543 5902 6061 518
Size: 648 3522 855 359 159 2042
Fragments arranged by size:
3522 2042 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3323 3943 5001 5678 6826 7071 1606
Size: 1717 620 1058 677 1148 245 2120
Fragments arranged by size:
2120 1717 1148 1058 677 620 245
NcoI C'CATG_G
Cuts at: 513 1065 3009 513
Size: 552 1944 5089
Fragments arranged by size:
5089 1944 552
NdeI CA'TA_TG
Cuts at: 387 4678 387
Size: 4291 3294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7585
NotI GC'GGCC_GC
Cuts at: 5032 5032
Size: 7585
NsiI A_TGCA'T
Cuts at: 2105 2869 2105
Size: 764 6821
NspI r_CATG'y
Cuts at: 1132 1229 2713 3577 4943 6488 1132
Size: 97 1484 864 1366 1545 2229
Fragments arranged by size:
2229 1545 1484 1366 864 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7585
PciI A'CATG_T
Cuts at: 6484 6484
Size: 7585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 4132 4336 1108
Size: 2010 1014 204 4357
Fragments arranged by size:
4357 2010 1014 204
PfoI T'CCnGG_A
Cuts at: 3717 3717
Size: 7585
PmeI GTTT'AAAC
Cuts at: 4889 4889
Size: 7585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 7167 7199 5654
Size: 32 1481 32 6040
Fragments arranged by size:
6040 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7585
PsiI TTA'TAA
Cuts at: 2544 5155 2544
Size: 2611 4974
PspOMI G'GGCC_C
Cuts at: 3738 3738
Size: 7585
PstI C_TGCA'G
Cuts at: 830 3747 4937 830
Size: 2917 1190 3478
Fragments arranged by size:
3478 2917 1190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7585
PvuII CAG'CTG
Cuts at: 3943 3943
Size: 7585
SalI G'TCGA_C
Cuts at: 1936 4927 1936
Size: 2991 4594
SbfI CC_TGCA'GG
Cuts at: 3747 4937 3747
Size: 1190 6395
ScaI AGT'ACT
Cuts at: 2942 3446 5833 7545 2942
Size: 504 2387 1712 2982
Fragments arranged by size:
2982 2387 1712 504
SexAI A'CCwGG_T
Cuts at: 3141 3141
Size: 7585
SfcI C'TryA_G
Cuts at: 826 1045 3463 3743 4933 6068 6749 6940 826
Size: 219 2418 280 1190 1135 681 191 1471
Fragments arranged by size:
2418 1471 1190 1135 681 280 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7585
SmaI CCC'GGG
Cuts at: 1872 4912 1872
Size: 3040 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3176 3398 4169 4766 4773 5689
Size: 197 926 1233 222 771 597 7 916
Cuts at: 5689 6590 6852 820
Size: 901 262 1553
Fragments arranged by size:
1553 1233 926 916 901 771 597 262
222 197 7
SnaBI TAC'GTA
Cuts at: 493 5376 493
Size: 4883 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7585
SphI G_CATG'C
Cuts at: 1132 4943 1132
Size: 3811 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5509 5
Size: 47 4095 1362 2081
Fragments arranged by size:
4095 2081 1362 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7585
StyI C'CwwG_G
Cuts at: 513 1065 2192 3009 4725 5024 513
Size: 552 1127 817 1716 299 3074
Fragments arranged by size:
3074 1716 1127 817 552 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3853 4385 5612 5629 5782 5967
Size: 1255 1821 452 532 1227 17 153 185
Cuts at: 5967 6412 325
Size: 445 1498
Fragments arranged by size:
1821 1498 1255 1227 532 452 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2783 2940 3444
Size: 274 80 33 51 1443 806 157 504
Cuts at: 3444 5831 7543 96
Size: 2387 1712 138
Fragments arranged by size:
2387 1712 1443 806 504 274 157 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 4706 4738 7545 7577 2134
Size: 32 2540 32 2807 32 2142
Fragments arranged by size:
2807 2540 2142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7585
XbaI T'CTAG_A
Cuts at: 4921 4921
Size: 7585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 7585
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7585
XmaI C'CCGG_G
Cuts at: 1870 4910 1870
Size: 3040 4545
XmnI GAAnn'nnTTC
Cuts at: 2187 5714 2187
Size: 3527 4058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3045 4598 5393 276
Size: 53 83 186 936 1511 1553 795 2468
Fragments arranged by size:
2468 1553 1511 936 795 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI